MD10G1130700.v1.1

GPI mannosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0028200
Physical Location & Seq
Forward (+)
21113694 .. 21114475
782 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1130700.v1.1.491

Sequence Viewer

Length: 543 bp
ATGGGTGGGATTTCCTGTATGAAGCACTATTGTATCATCTTACCCGTACTGATCCAAGGCACAATTTTTCCGTTTACTTTTATCACATCTACCTTCAGTACCACCCTAAACTTAAAACTTTCGATATTGGAGAAACTCGTCTCATTTTTGCCTCAGTTGATAGTCCAGGTTGTTCTTGTTTTTAGTTTCGCCAAAGACCTTCCATTCTGCTGCTTAGTACAGACAGTGGCATTCGTGGCATTCAACAAGGTGATGACTGCACAATACTTTGTGTGGTTCTTTTGTCTCTTGCCGCTGGTTCTCGCTTGGAGCAACATGAAGCTTAAATGGAAAGGCCTATCGTGCATCGCATTGTGGGTAGGCTGTCAACTTCATTGGTTGTTTTGGGGTTATATGCTTGAGTTCAAAGGACAAAATGTCTTTCTTCAACTCTGGATTGCAAGCATCATATTCTTAGCTGCAAACACGTACGTCTTAGTTTCGCTCATCCGCGACCACATATTTTCTCCAGTTTTCGCACAGTTGGAGAAAACCTCCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Mannosyl_trans

Protein Analysis

181

Amino Acids

20.63

Weight (kDa)

9.04

Isoelectric Point (pI)

38.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mannosyl_trans PF05007 42 - 163 1.6e-36 Mannosyltransferase (PIG-M)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028200)

Species Orthologous Gene IDs
malus_domestica MD10G1130700.v1.1
pyrus_communis pycom05g12310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 492
AciI CCGC 2 cut(s) 293, 490
AclWI GGATC 1 cut(s) 46
AcuI CTGAAG 1 cut(s) 79
AfaI GTAC 4 cut(s) 48, 100, 219, 470
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 3 cut(s) 244, 406, 428
AhdI GACNNNNNGTC 1 cut(s) 416
AjnI CCWGG 1 cut(s) 165
AluBI AGCT 2 cut(s) 322, 458
AluI AGCT 2 cut(s) 322, 458
Alw26I GTCTC 2 cut(s) 145, 290
AlwI GGATC 1 cut(s) 46
AoxI GGCC 1 cut(s) 334
ApeKI GCWGC 2 cut(s) 210, 458
AsuHPI GGTGA 1 cut(s) 262
BaeI ACNNNNGTAYC 2 cut(s) 16, 49
BbvI GCAGC 2 cut(s) 197, 445
BciT130I CCWGG 1 cut(s) 167
BcoDI GTCTC 2 cut(s) 145, 290
BisI GCNGC 3 cut(s) 211, 293, 459
BlsI GCNGC 3 cut(s) 212, 294, 460
Bme1390I CCNGG 1 cut(s) 167
BmeRI GACNNNNNGTC 1 cut(s) 416
BmrFI CCNGG 1 cut(s) 167
BmsI GCATC 2 cut(s) 354, 453
BplI GAGNNNNNCTC 1 cut(s) 518
BpmI CTGGAG 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 419
BsaAI YACGTR 1 cut(s) 468
BsaJI CCNNGG 1 cut(s) 55
Bse1I ACTGG 1 cut(s) 509
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 1 cut(s) 55
BseGI GGATG 1 cut(s) 486
BseMII CTCAG 1 cut(s) 167
BseNI ACTGG 1 cut(s) 509
BseXI GCAGC 2 cut(s) 197, 445
BsgI GTGCAG 1 cut(s) 243
Bsh1236I CGCG 1 cut(s) 492
BshFI GGCC 1 cut(s) 336
BsiWI CGTACG 1 cut(s) 468
BsmAI GTCTC 2 cut(s) 145, 290
BsmBI CGTCTC 1 cut(s) 145
BsmI GAATGC 2 cut(s) 230, 239
BsnI GGCC 1 cut(s) 336
Bsp143I GATC 1 cut(s) 51
BspACI CCGC 2 cut(s) 293, 490
BspANI GGCC 1 cut(s) 336
BspCNI CTCAG 1 cut(s) 166
BspFNI CGCG 1 cut(s) 492
BspPI GGATC 1 cut(s) 46
BsrI ACTGG 1 cut(s) 509
BssECI CCNNGG 1 cut(s) 55
BssMI GATC 1 cut(s) 51
BssT1I CCWWGG 1 cut(s) 55
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 2 cut(s) 226, 522
BstBAI YACGTR 1 cut(s) 468
BstC8I GCNNGC 1 cut(s) 442
BstDEI CTNAG 4 cut(s) 153, 214, 454, 475
BstF5I GGATG 1 cut(s) 486
BstFNI CGCG 1 cut(s) 492
BstKTI GATC 1 cut(s) 54
BstMAI GTCTC 2 cut(s) 145, 290
BstMBI GATC 1 cut(s) 51
BstMWI GCNNNNNNNGC 2 cut(s) 236, 342
BstNI CCWGG 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 165
BstUI CGCG 1 cut(s) 492
BstV1I GCAGC 2 cut(s) 197, 445
BsuRI GGCC 1 cut(s) 336
BtgZI GCGATG 1 cut(s) 331
BtsCI GGATG 1 cut(s) 486
BtsIMutI CAGTG 1 cut(s) 231
Cac8I GCNNGC 1 cut(s) 442
Csp6I GTAC 4 cut(s) 47, 99, 218, 469
CviAII CATG 2 cut(s) 316, 540
CviJI RGCY 4 cut(s) 322, 336, 363, 458
CviKI_1 RGCY 4 cut(s) 322, 336, 363, 458
CviQI GTAC 4 cut(s) 47, 99, 218, 469
DdeI CTNAG 4 cut(s) 153, 214, 454, 475
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
DriI GACNNNNNGTC 1 cut(s) 416
Eam1105I GACNNNNNGTC 1 cut(s) 416
Eco130I CCWWGG 1 cut(s) 55
Eco147I AGGCCT 1 cut(s) 336
Eco57I CTGAAG 1 cut(s) 79
EcoRII CCWGG 1 cut(s) 165
EcoT14I CCWWGG 1 cut(s) 55
ErhI CCWWGG 1 cut(s) 55
Esp3I CGTCTC 1 cut(s) 145
FaeI CATG 2 cut(s) 319, 543
FaiI YATR 7 cut(s) 20, 317, 393, 395, 449, 500, 541
FatI CATG 2 cut(s) 315, 539
Fnu4HI GCNGC 3 cut(s) 211, 293, 459
FokI GGATG 1 cut(s) 473
Fsp4HI GCNGC 3 cut(s) 211, 293, 459
GluI GCNGC 3 cut(s) 211, 293, 459
GsuI CTGGAG 1 cut(s) 492
HaeIII GGCC 1 cut(s) 336
Hin1II CATG 2 cut(s) 319, 543
HincII GTYRAC 1 cut(s) 368
HindII GTYRAC 1 cut(s) 368
HindIII AAGCTT 1 cut(s) 320
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 2 cut(s) 75, 368
Hpy188III TCNNGA 1 cut(s) 433
Hpy8I GTNNAC 2 cut(s) 75, 368
HpyAV CCTTC 2 cut(s) 103, 209
HpyCH4III ACNGT 2 cut(s) 226, 522
HpyCH4IV ACGT 2 cut(s) 467, 471
HpyCH4V TGCA 4 cut(s) 260, 345, 440, 461
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 342
HpyF3I CTNAG 4 cut(s) 153, 214, 454, 475
HpySE526I ACGT 2 cut(s) 467, 471
Hsp92II CATG 2 cut(s) 319, 543
Kzo9I GATC 1 cut(s) 51
LmnI GCTCC 1 cut(s) 309
LpnPI CCDG 6 cut(s) 28, 152, 179, 281, 418, 522
Lsp1109I GCAGC 2 cut(s) 197, 445
LweI GCATC 2 cut(s) 354, 453
MaeII ACGT 2 cut(s) 467, 471
MalI GATC 1 cut(s) 53
MboI GATC 1 cut(s) 51
MboII GAAGA 1 cut(s) 416
MluCI AATT 1 cut(s) 63
MmeI TCCRAC 1 cut(s) 504
MnlI CCTC 1 cut(s) 162
MseI TTAA 2 cut(s) 113, 324
MspA1I CMGCKG 1 cut(s) 295
MspR9I CCNGG 1 cut(s) 167
Mva1269I GAATGC 2 cut(s) 230, 239
MvaI CCWGG 1 cut(s) 167
MvnI CGCG 1 cut(s) 492
MwoI GCNNNNNNNGC 2 cut(s) 236, 342
NdeII GATC 1 cut(s) 51
NlaIII CATG 2 cut(s) 319, 543
PceI AGGCCT 1 cut(s) 336
PctI GAATGC 2 cut(s) 230, 239
Pfl23II CGTACG 1 cut(s) 468
PkrI GCNGC 3 cut(s) 212, 294, 460
Ppu21I YACGTR 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 165
PspGI CCWGG 1 cut(s) 165
PspLI CGTACG 1 cut(s) 468
RsaI GTAC 4 cut(s) 48, 100, 219, 470
RsaNI GTAC 4 cut(s) 47, 99, 218, 469
SaqAI TTAA 2 cut(s) 113, 324
SatI GCNGC 3 cut(s) 211, 293, 459
Sau3AI GATC 1 cut(s) 51
ScrFI CCNGG 1 cut(s) 167
SetI ASST 9 cut(s) 95, 171, 201, 252, 324, 460, 470, 474, 536
SfaNI GCATC 2 cut(s) 354, 453
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 1 cut(s) 63
SseBI AGGCCT 1 cut(s) 336
SsiI CCGC 2 cut(s) 293, 490
StuI AGGCCT 1 cut(s) 336
StyD4I CCNGG 1 cut(s) 165
StyI CCWWGG 1 cut(s) 55
TaaI ACNGT 2 cut(s) 226, 522
TaiI ACGT 2 cut(s) 470, 474
TaqI TCGA 1 cut(s) 122
TasI AATT 1 cut(s) 63
TatI WGTACW 1 cut(s) 217
TauI GCSGC 1 cut(s) 295
Tru1I TTAA 2 cut(s) 113, 324
Tru9I TTAA 2 cut(s) 113, 324
TscAI CASTG 1 cut(s) 231
TseI GCWGC 2 cut(s) 210, 458
TspDTI ATGAA 3 cut(s) 35, 332, 362
TspGWI ACGGA 1 cut(s) 60
TspRI CASTG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.