pycom05g12310

GPI mannosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0028200
Physical Location & Seq
Forward (+)
15767634 .. 15767879
246 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g12310.1

Sequence Viewer

Length: 246 bp
ATGAAGCTTAAATGGAAAGGTGTATCATGCATCGCATTGTGGGTAGGCGGTCAACTTCATTGGTTGTTGTGGGGTTATATGCTTGAGTTTAAGGGACAAAACGTCTTTCTTCAACTCTGGATGGCAAGCATCATATTCTTAGCTGCAAACACGTACGTTTTAGTTTCTCTCATCCGCGATCACAGATTTTCTCCAGTTTTCGCACCCTTGGAGAAAACCTCCAAGGACTCGAAGAAACTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Mannosyl_trans

Protein Analysis

82

Amino Acids

9.43

Weight (kDa)

9.93

Isoelectric Point (pI)

38.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mannosyl_trans PF05007 5 - 58 2.8e-13 Mannosyltransferase (PIG-M)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028200)

Species Orthologous Gene IDs
malus_domestica MD10G1130700.v1.1
pyrus_communis pycom05g12310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 177
AciI CCGC 2 cut(s) 48, 175
AfaI GTAC 1 cut(s) 155
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 1 cut(s) 113
AhdI GACNNNNNGTC 1 cut(s) 101
AluBI AGCT 2 cut(s) 7, 143
AluI AGCT 2 cut(s) 7, 143
ApeKI GCWGC 1 cut(s) 143
BbvI GCAGC 1 cut(s) 130
BccI CCATC 1 cut(s) 115
BisI GCNGC 1 cut(s) 144
BlsI GCNGC 1 cut(s) 145
BmeRI GACNNNNNGTC 1 cut(s) 101
BmsI GCATC 2 cut(s) 39, 138
BplI GAGNNNNNCTC 2 cut(s) 203, 235
BpmI CTGGAG 1 cut(s) 177
BpuEI CTTGAG 2 cut(s) 104, 224
BsaAI YACGTR 1 cut(s) 153
BsaJI CCNNGG 2 cut(s) 207, 222
Bse1I ACTGG 1 cut(s) 194
BseDI CCNNGG 2 cut(s) 207, 222
BseGI GGATG 2 cut(s) 126, 171
BseNI ACTGG 1 cut(s) 194
BseXI GCAGC 1 cut(s) 130
Bsh1236I CGCG 1 cut(s) 177
BsiWI CGTACG 1 cut(s) 153
BslFI GGGAC 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 108
Bsp143I GATC 1 cut(s) 178
BspACI CCGC 2 cut(s) 48, 175
BspFNI CGCG 1 cut(s) 177
BsrI ACTGG 1 cut(s) 194
BssECI CCNNGG 2 cut(s) 207, 222
BssMI GATC 1 cut(s) 178
BssT1I CCWWGG 2 cut(s) 207, 222
BstBAI YACGTR 1 cut(s) 153
BstC8I GCNNGC 1 cut(s) 127
BstDEI CTNAG 1 cut(s) 139
BstF5I GGATG 2 cut(s) 126, 171
BstFNI CGCG 1 cut(s) 177
BstKTI GATC 1 cut(s) 181
BstMBI GATC 1 cut(s) 178
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 1 cut(s) 130
BtgZI GCGATG 1 cut(s) 16
BtsCI GGATG 2 cut(s) 126, 171
Cac8I GCNNGC 1 cut(s) 127
Csp6I GTAC 1 cut(s) 154
CviAII CATG 1 cut(s) 27
CviJI RGCY 2 cut(s) 7, 143
CviKI_1 RGCY 2 cut(s) 7, 143
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 1 cut(s) 139
DpnI GATC 1 cut(s) 180
DpnII GATC 1 cut(s) 178
DriI GACNNNNNGTC 1 cut(s) 101
Eam1105I GACNNNNNGTC 1 cut(s) 101
Eco130I CCWWGG 2 cut(s) 207, 222
EcoT14I CCWWGG 2 cut(s) 207, 222
EcoT22I ATGCAT 1 cut(s) 32
ErhI CCWWGG 2 cut(s) 207, 222
FaeI CATG 1 cut(s) 30
FaiI YATR 4 cut(s) 28, 78, 80, 134
FaqI GGGAC 1 cut(s) 108
FatI CATG 1 cut(s) 26
Fnu4HI GCNGC 1 cut(s) 144
FokI GGATG 2 cut(s) 133, 158
Fsp4HI GCNGC 1 cut(s) 144
GluI GCNGC 1 cut(s) 144
GsuI CTGGAG 1 cut(s) 177
Hin1II CATG 1 cut(s) 30
HincII GTYRAC 1 cut(s) 53
HindII GTYRAC 1 cut(s) 53
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 1 cut(s) 227
Hpy166II GTNNAC 1 cut(s) 53
Hpy188III TCNNGA 1 cut(s) 118
Hpy8I GTNNAC 1 cut(s) 53
HpyCH4IV ACGT 3 cut(s) 102, 152, 156
HpyCH4V TGCA 2 cut(s) 30, 146
HpyF3I CTNAG 1 cut(s) 139
HpySE526I ACGT 3 cut(s) 102, 152, 156
Hsp92II CATG 1 cut(s) 30
Kzo9I GATC 1 cut(s) 178
LpnPI CCDG 2 cut(s) 103, 207
Lsp1109I GCAGC 1 cut(s) 130
LweI GCATC 2 cut(s) 39, 138
MaeII ACGT 3 cut(s) 102, 152, 156
MalI GATC 1 cut(s) 180
MboI GATC 1 cut(s) 178
MboII GAAGA 2 cut(s) 101, 244
MlyI GAGTC 1 cut(s) 221
MnlI CCTC 1 cut(s) 229
Mph1103I ATGCAT 1 cut(s) 32
MseI TTAA 2 cut(s) 9, 90
MvnI CGCG 1 cut(s) 177
NdeII GATC 1 cut(s) 178
NlaIII CATG 1 cut(s) 30
NsiI ATGCAT 1 cut(s) 32
Pfl23II CGTACG 1 cut(s) 153
PkrI GCNGC 1 cut(s) 145
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
Ppu21I YACGTR 1 cut(s) 153
PspLI CGTACG 1 cut(s) 153
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
SaqAI TTAA 2 cut(s) 9, 90
SatI GCNGC 1 cut(s) 144
Sau3AI GATC 1 cut(s) 178
SchI GAGTC 1 cut(s) 221
SetI ASST 7 cut(s) 9, 22, 105, 145, 155, 159, 221
SfaNI GCATC 2 cut(s) 39, 138
SmlI CTYRAG 2 cut(s) 83, 239
SmoI CTYRAG 2 cut(s) 83, 239
SsiI CCGC 2 cut(s) 48, 175
StyI CCWWGG 2 cut(s) 207, 222
TaiI ACGT 3 cut(s) 105, 155, 159
TaqI TCGA 1 cut(s) 230
Tru1I TTAA 2 cut(s) 9, 90
Tru9I TTAA 2 cut(s) 9, 90
TseI GCWGC 1 cut(s) 143
TspDTI ATGAA 2 cut(s) 17, 47
Zsp2I ATGCAT 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.