Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0020965

Physical Location & Seq

Genomic Coordinates Forward (+)

24595490 .. 24598201

Gene Structure 2712 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1157300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2712 bp

ATGTTGGTTCGCACAAACAATGTCCGAAACGTCCTTCTGAGAGCTCCCTATTTGTTCGAACTTCCTCGGACGCTTTCCTCCGTCGCATACCAATCTTCCGGCAGCCGAGCAGCCCCAGAAAGCTCAGATGACACGACTTCTCGCGTATCGTCTCTGATTCAGCAACCCAATTGGGAAAGGAGCAGTCTTCTCAAGTCTATGGTCTCCCACATGGCACCACAAGCAGCCGCCAAGGTTTTACAGCTCCACGGCGGCGACACCGAACTCGGGGTTCGCTTTTTCAAGTGGGTTTGTAAGCATTCTACTTATTGTTATGATTTGGATAGCAGAATCGCTCTGTTGAATTTGTTGGTTTCTTGTGAAAGTTTATATGGGCTTGCTCAGAAAGCCATTGCTTTGTTAATCAAGGAGTTAAACAATAGTGAGCCTGAGATTATGAGGCTGATGGGGGCGTTAGAAAATATGCGAAAGGTTGGATTTTGGTTGAGTTATCCTTGTTATAGTAGCCTTTTGATGTCTTTAGCTAGGTTGGATTTGGGGTTTTTGACTTTTTTGGTGTATAAAAAGATGCTTAGTGATGGGTTTCTTCTTGGTGTTATTGACTACAGAAATGTAATTAATGCTCTATGCAAAAATGGGTTTGTGCAATCTGCTGAAATGTTTGTGTGTAGGGTTTTGAAACTCGGGTTTCGGTTGGACACTCATATTTGTACTTCTTTGGTGTTGGGTAATTGTCGAGAGAGTCATCTCCAGGAAGCCTCCCGGGTGTTCGATATAATGTCTAAGAGTCATGGTTGTGAGCCTAATTCGGTGACGTACTCGATTTTGGTTCATGGTTACTGTGAAATTGATAAGCTTGATGAAGCTTTTCGTCTGAAAGAAGAGATGAGTGAGAAGGGCTGCCAGCCGAGTACTCGTACATTTACTGTACTCATCAAGGCCTTGTGTGACATTGGGTCGACTGATAAGGCTTTAGGGTTGCTTGATGAGATGGTTACAAAGGGTTGTAAACCGAATGTTCATACTTATACCGTATTGATTGATAGATTATGCAGGGAAGGAAAGATTGAGGAGGCAAATGGAATGTTCAGAAAGATGCTTGCAGATGAGCTTTTTCCTGGCACAGTAACTTACAATGCACTGATAAATGGATACTGCAAAGAAGGACGTGTAATTTCTGCTTTTGAGCTCCTTGGTGTGATGGAGAAAAGACTATGTAAGCCTAACATTCGAACCTACAATGAGCTCATCGAAGGGCTATGCAAGGTTTATAAAACTTACAAAGCCATGTTTCTTTTGAAAAGAATAGTTGATAATGGTTTGTTACCAAATAGGGTTACCTACAATATTTTGATCGATGGGTTTTGCAAAGAAGGTCAACTTGGATTGGCTTTTGAAACGTTTAAGTCAATGAGCTCATTTGGTATTGAACCAGATTGTTTTTCTTTCACTGCCTTGATTGATGGCCTCTGTAAGCAAGGGAGACCGGGGAATGCAAGCAGCATTTTAGGTTTAATGGTAAAGAAGGGGATTTCCCCTGATGAAGTAACTATGACGGCTCTTATTGACGGGTATTGCAAGAATGACGAAATTGGAAACGCATCGATGCTTTTTGAGAAGATGGTGGAGAAGAAGAAATTGACAACACCACACACTTTCAATTGTTTTCTTGATATTCTCAGCAAAGATGATAAGGTGCATGCGGCACAGGCAATGTTGGGGAAGATGCTGAAGTATGGATCAGTTCCATCTGTTGTGACTTATACAATACTAGTCGATGGGCTTTGCCAAACAGGTGATATTATGTGTGCACTAAAAATGTTGGATTTGATGAGGCGAACTAGCCGCCCTCCAAATGTCTACACCTACACAGTCGTGATCAATGGTCTCTGCTTGAACGGAAGAGTGGAGGAAGCAGAAAAGCTTCTCTTTAGCATGTCTGATTTCGGTATACCTCCAAACCACGTTACATATACTGTATTAGTTAAAGGTCTTGTTAATGCTGGTAGGTTGGATCATGCCTTTGAGATTTTGAGTGTTATGGTTCAAAAAGGTTTTCAGCCAAACGCTCGAATCTATTCTGCGCTTCTTGCAGGTCTTGTTTTGTCAAACGAGGCCAAGGAAGATGCCTGTTCATATATGTCTTCCACTTCTACAGATGCTACACCATTACTTCCGAAGGACATTGACGACAATTGCATTTCTAGCTATGCTTTTAAAAATATGGATATTGAACATGCTTTCAGTCTTGAAGAAAAGGTCAGAAAATGTGGCTGCTCTACAATGGATTTGTACAACTTTGTTGTCATGGGATTATGCAGGGAAGCAAGGGTTGCAGAAGCAGATCGTATCATCAACAAAGTACTAAAATGCGGCGTGTTTCCTCAGAAGGCTGTTTGTGCACTCATCAACAGTTATTGCAAGGAGCGCCGATATGATCATTGCCTCGATTTCATGACAACAATTCTGAATCAAGGGTTTGTTCCATCCGTGTTATCATATTGTTCGGTGATTCAGGGACTTTACAGTGAAGGGAGGGCTGAACAAGGTGAAGAACTTTTCTCAGAACTATTAAGACACAATGATATCAAGGAAAAGTCCTCAGTTTTGCCATATTTGGAAATCCTAGTAAAGAAAGAGGAACCTGAGGATTGCCTTCACATTCTCAAACTAATTGAGCAAATGGGTTGCGGAGAGAGGCCAATTATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

904

Amino Acids

101.0

Weight (kDa)

7.14

Isoelectric Point (pI)

36.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	233 - 281	5.6e-11	PPR repeat family
PPR_long	PF17177	236 - 352	3.7e-09	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	253 - 365	4e-09	Fungal tetratrico peptide repeats
PPR_1	PF12854	264 - 297	2.3e-12	PPR repeat
PPR_3	PF13812	265 - 315	5.2e-07	Pentatricopeptide repeat domain
PPR	PF01535	271 - 301	3.8e-06	PPR repeat
PPR_2	PF13041	276 - 317	2e-12	PPR repeat family
PPR_1	PF12854	299 - 332	9.6e-09	PPR repeat
PPR_2	PF13041	311 - 352	3.2e-13	PPR repeat family
PPR_3	PF13812	327 - 386	8.8e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	334 - 366	8.6e-12	PPR repeat
PPR	PF01535	342 - 368	6e-06	PPR repeat
PPR_3	PF13812	363 - 418	6e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	371 - 402	2.6e-11	PPR repeat
PPR_2	PF13041	373 - 422	1.7e-17	PPR repeat family
TPR_24	PF23276	376 - 471	3.7e-06	Fungal tetratrico peptide repeats
PPR	PF01535	376 - 404	6.5e-08	PPR repeat
PPR_3	PF13812	432 - 488	3e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	439 - 471	5.7e-12	PPR repeat
PPR_2	PF13041	443 - 492	2.5e-17	PPR repeat family
PPR	PF01535	446 - 476	1.3e-07	PPR repeat
PPR_1	PF12854	475 - 506	2.5e-07	PPR repeat
PPR_2	PF13041	482 - 527	1.1e-12	PPR repeat family
PPR_1	PF12854	509 - 542	3e-08	PPR repeat
PPR_2	PF13041	513 - 558	2.2e-08	PPR repeat family
PPR_2	PF13041	551 - 597	3e-10	PPR repeat family
PPR_1	PF12854	580 - 612	8.1e-10	PPR repeat
PPR_2	PF13041	583 - 631	6.1e-15	PPR repeat family
PPR_long	PF17177	594 - 706	3.2e-07	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	618 - 646	3.6e-10	PPR repeat
PPR	PF01535	621 - 651	6e-07	PPR repeat
PPR_2	PF13041	626 - 667	4e-10	PPR repeat family
PPR_3	PF13812	642 - 697	1.5e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	650 - 682	6.8e-08	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020965)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07290
malus_domestica	MD10G1157300.v1.1
prunus_persica	Prupe.8G196100_v2.0.a1 Prupe.8G196100_v2.0.a1
pyrus_communis	pycom10g13580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1272
Acc36I	ACCTGC	1 cut(s)	2084
AccB1I	GGYRCC	1 cut(s)	214
AccI	GTMKAC	3 cut(s)	959, 1860, 1951
AccII	CGCG	1 cut(s)	144
AciI	CCGC	6 cut(s)	228, 252, 1703, 1846, 2373, 2691
AclI	AACGTT	1 cut(s)	1400
AclWI	GGATC	2 cut(s)	1747, 2022
AcsI	RAATTY	1 cut(s)	343
AcuI	CTGAAG	1 cut(s)	1751
AfaI	GTAC	7 cut(s)	712, 818, 913, 919, 930, 2294, 2364
AfiI	CCNNNNNNNGG	1 cut(s)	267
AflIII	ACRYGT	1 cut(s)	1168
AhdI	GACNNNNNGTC	1 cut(s)	955
AhlI	ACTAGT	1 cut(s)	1771
AjiI	CACGTC	1 cut(s)	1169
AjnI	CCWGG	2 cut(s)	750, 1117
AjuI	GAANNNNNNNTTGG	2 cut(s)	1365, 1397
AleI	CACNNNNGTG	1 cut(s)	1874
Alw21I	GWGCWC	6 cut(s)	46, 1191, 1248, 1418, 1813, 2404
Alw26I	GTCTC	4 cut(s)	156, 208, 1477, 1892
Alw44I	GTGCAC	2 cut(s)	1809, 2400
AlwI	GGATC	2 cut(s)	1747, 2022
Ama87I	CYCGRG	3 cut(s)	266, 683, 762
AoxI	GGCC	4 cut(s)	939, 1465, 2115, 2699
ApaLI	GTGCAC	2 cut(s)	1809, 2400
ApeKI	GCWGC	6 cut(s)	102, 110, 224, 900, 1500, 2274
ApoI	RAATTY	1 cut(s)	343
AseI	ATTAAT	1 cut(s)	618
Asp700I	GAANNNNTTC	3 cut(s)	867, 1923, 2077
AspLEI	GCGC	2 cut(s)	2086, 2430
AsuC2I	CCSGG	3 cut(s)	763, 764, 1488
AsuHPI	GGTGA	4 cut(s)	823, 1808, 2521, 2561
AsuII	TTCGAA	2 cut(s)	57, 1231
AvaI	CYCGRG	3 cut(s)	266, 683, 762
AxyI	CCTNAGG	1 cut(s)	2646
BaeGI	GKGCMC	2 cut(s)	1813, 2404
BanI	GGYRCC	1 cut(s)	214
BanII	GRGCYC	4 cut(s)	46, 1191, 1248, 1418
BbsI	GAAGAC	2 cut(s)	179, 2136
Bbv12I	GWGCWC	6 cut(s)	46, 1191, 1248, 1418, 1813, 2404
BbvI	GCAGC	6 cut(s)	114, 122, 236, 887, 1512, 2261
BceAI	ACGGC	2 cut(s)	265, 1572
BciT130I	CCWGG	2 cut(s)	752, 1119
BciVI	GTATCC	1 cut(s)	1145
BclI	TGATCA	2 cut(s)	1878, 2437
BcnI	CCSGG	3 cut(s)	763, 764, 1488
BcoDI	GTCTC	4 cut(s)	156, 208, 1477, 1892
BcuI	ACTAGT	1 cut(s)	1771
BfaI	CTAG	5 cut(s)	525, 1772, 1842, 2205, 2627
BfmI	CTRYAG	2 cut(s)	604, 2154
BfoI	RGCGCY	1 cut(s)	2431
BfuAI	ACCTGC	1 cut(s)	2084
BfuI	GTATCC	1 cut(s)	1145
BmcAI	AGTACT	2 cut(s)	913, 2364
Bme1390I	CCNGG	5 cut(s)	752, 763, 764, 1119, 1488
BmeRI	GACNNNNNGTC	1 cut(s)	955
BmeT110I	CYCGRG	3 cut(s)	266, 683, 762
BmgBI	CACGTC	1 cut(s)	1169
BmiI	GGNNCC	2 cut(s)	216, 2643
BmrFI	CCNGG	5 cut(s)	752, 763, 764, 1119, 1488
BmsI	GCATC	7 cut(s)	558, 1086, 1596, 1610, 1716, 2116, 2149
BpiI	GAAGAC	2 cut(s)	179, 2136
BpmI	CTGGAG	1 cut(s)	734
Bpu14I	TTCGAA	2 cut(s)	57, 1231
BpuEI	CTTGAG	1 cut(s)	176
BpuMI	CCSGG	3 cut(s)	763, 764, 1488
Bsa29I	ATCGAT	2 cut(s)	1356, 1604
BsaI	GGTCTC	3 cut(s)	208, 1477, 1892
BsaJI	CCNNGG	7 cut(s)	65, 231, 247, 762, 1192, 1487, 2118
Bsc4I	CCNNNNNNNGG	1 cut(s)	267
Bse21I	CCTNAGG	1 cut(s)	2646
Bse3DI	GCAATG	3 cut(s)	390, 1719, 2440
BseBI	CCWGG	2 cut(s)	752, 1119
BseCI	ATCGAT	2 cut(s)	1356, 1604
BseDI	CCNNGG	7 cut(s)	65, 231, 247, 762, 1192, 1487, 2118
BseGI	GGATG	1 cut(s)	2486
BseLI	CCNNNNNNNGG	1 cut(s)	267
BseMI	GCAATG	3 cut(s)	390, 1719, 2440
BseMII	CTCAG	9 cut(s)	29, 138, 395, 420, 1693, 2399, 2577, 2616, 2637
BseRI	GAGGAG	1 cut(s)	1085
BseSI	GKGCMC	2 cut(s)	1813, 2404
BseXI	GCAGC	6 cut(s)	114, 122, 236, 887, 1512, 2261
Bsh1236I	CGCG	1 cut(s)	144
BshFI	GGCC	4 cut(s)	941, 1467, 2117, 2701
BshNI	GGYRCC	1 cut(s)	214
BshVI	ATCGAT	2 cut(s)	1356, 1604
BsiHKAI	GWGCWC	6 cut(s)	46, 1191, 1248, 1418, 1813, 2404
BsiHKCI	CYCGRG	3 cut(s)	266, 683, 762
BsiSI	CCGG	3 cut(s)	99, 763, 1487
BslFI	GGGAC	1 cut(s)	2532
BslI	CCNNNNNNNGG	1 cut(s)	267
BsmAI	GTCTC	4 cut(s)	156, 208, 1477, 1892
BsmBI	CGTCTC	1 cut(s)	156
BsmFI	GGGAC	1 cut(s)	2532
BsmI	GAATGC	2 cut(s)	298, 1498
BsnI	GGCC	4 cut(s)	941, 1467, 2117, 2701
Bso31I	GGTCTC	3 cut(s)	208, 1477, 1892
BsoBI	CYCGRG	3 cut(s)	266, 683, 762
Bsp119I	TTCGAA	2 cut(s)	57, 1231
Bsp1286I	GDGCHC	6 cut(s)	46, 1191, 1248, 1418, 1813, 2404
Bsp1407I	TGTACA	1 cut(s)	2292
Bsp143I	GATC	6 cut(s)	1353, 1739, 1878, 2014, 2344, 2437
BspACI	CCGC	6 cut(s)	228, 252, 1703, 1846, 2373, 2691
BspANI	GGCC	4 cut(s)	941, 1467, 2117, 2701
BspCNI	CTCAG	9 cut(s)	30, 137, 394, 421, 1692, 2398, 2576, 2615, 2638
BspDI	ATCGAT	2 cut(s)	1356, 1604
BspFNI	CGCG	1 cut(s)	144
BspHI	TCATGA	1 cut(s)	2454
BspLI	GGNNCC	2 cut(s)	216, 2643
BspMI	ACCTGC	1 cut(s)	2084
BspPI	GGATC	2 cut(s)	1747, 2022
BspT104I	TTCGAA	2 cut(s)	57, 1231
BspT107I	GGYRCC	1 cut(s)	214
BspTNI	GGTCTC	3 cut(s)	208, 1477, 1892
BsrDI	GCAATG	3 cut(s)	390, 1719, 2440
BsrGI	TGTACA	1 cut(s)	2292
BssECI	CCNNGG	7 cut(s)	65, 231, 247, 762, 1192, 1487, 2118
BssMI	GATC	6 cut(s)	1353, 1739, 1878, 2014, 2344, 2437
BssNAI	GTATAC	1 cut(s)	1952
BssT1I	CCWWGG	3 cut(s)	231, 1192, 2118
Bst1107I	GTATAC	1 cut(s)	1952
Bst2UI	CCWGG	2 cut(s)	752, 1119
Bst4CI	ACNGT	8 cut(s)	842, 928, 1033, 1126, 1873, 1978, 2414, 2528
Bst6I	CTCTTC	2 cut(s)	876, 1897
BstAPI	GCANNNNNTGC	1 cut(s)	2333
BstAUI	TGTACA	1 cut(s)	2292
BstBI	TTCGAA	2 cut(s)	57, 1231
BstC8I	GCNNGC	5 cut(s)	378, 905, 1101, 1498, 1701
BstDSI	CCRYGG	1 cut(s)	247
BstEII	GGTNACC	1 cut(s)	1336
BstF5I	GGATG	1 cut(s)	2486
BstFNI	CGCG	1 cut(s)	144
BstH2I	RGCGCY	1 cut(s)	2431
BstHHI	GCGC	2 cut(s)	2086, 2430
BstKTI	GATC	6 cut(s)	1356, 1742, 1881, 2017, 2347, 2440
BstMAI	GTCTC	4 cut(s)	156, 208, 1477, 1892
BstMBI	GATC	6 cut(s)	1353, 1739, 1878, 2014, 2344, 2437
BstMWI	GCNNNNNNNGC	8 cut(s)	221, 386, 1709, 2090, 2205, 2333, 2399, 2427
BstNI	CCWGG	2 cut(s)	752, 1119
BstNSI	RCATGY	3 cut(s)	1703, 1939, 2240
BstPI	GGTNACC	1 cut(s)	1336
BstSCI	CCNGG	5 cut(s)	750, 761, 762, 1117, 1486
BstSFI	CTRYAG	2 cut(s)	604, 2154
BstSLI	GKGCMC	2 cut(s)	1813, 2404
BstUI	CGCG	1 cut(s)	144
BstV1I	GCAGC	6 cut(s)	114, 122, 236, 887, 1512, 2261
BstV2I	GAAGAC	2 cut(s)	179, 2136
BstZ17I	GTATAC	1 cut(s)	1952
Bsu15I	ATCGAT	2 cut(s)	1356, 1604
Bsu36I	CCTNAGG	1 cut(s)	2646
BsuI	GTATCC	1 cut(s)	1145
BsuRI	GGCC	4 cut(s)	941, 1467, 2117, 2701
BsuTUI	ATCGAT	2 cut(s)	1356, 1604
BtgI	CCRYGG	1 cut(s)	247
BtrI	CACGTC	1 cut(s)	1169
BtsCI	GGATG	1 cut(s)	2486
BtsI	GCAGTG	1 cut(s)	1449
BtsIMutI	CAGTG	3 cut(s)	1139, 1449, 2533
BveI	ACCTGC	1 cut(s)	2084
Cac8I	GCNNGC	5 cut(s)	378, 905, 1101, 1498, 1701
CciI	TCATGA	1 cut(s)	2454
CfoI	GCGC	2 cut(s)	2086, 2430
Cfr9I	CCCGGG	1 cut(s)	762
ClaI	ATCGAT	2 cut(s)	1356, 1604
CseI	GACGC	1 cut(s)	79
Csp6I	GTAC	7 cut(s)	711, 817, 912, 918, 929, 2293, 2363
CviQI	GTAC	7 cut(s)	711, 817, 912, 918, 929, 2293, 2363
DpnI	GATC	6 cut(s)	1355, 1741, 1880, 2016, 2346, 2439
DpnII	GATC	6 cut(s)	1353, 1739, 1878, 2014, 2344, 2437
DraI	TTTAAA	1 cut(s)	2218
DriI	GACNNNNNGTC	1 cut(s)	955
Eam1104I	CTCTTC	2 cut(s)	876, 1897
Eam1105I	GACNNNNNGTC	1 cut(s)	955
EarI	CTCTTC	2 cut(s)	876, 1897
Ecl136II	GAGCTC	4 cut(s)	44, 1189, 1246, 1416
Eco130I	CCWWGG	3 cut(s)	231, 1192, 2118
Eco147I	AGGCCT	1 cut(s)	941
Eco24I	GRGCYC	4 cut(s)	46, 1191, 1248, 1418
Eco31I	GGTCTC	3 cut(s)	208, 1477, 1892
Eco32I	GATATC	1 cut(s)	2587
Eco53kI	GAGCTC	4 cut(s)	44, 1189, 1246, 1416
Eco57I	CTGAAG	1 cut(s)	1751
Eco81I	CCTNAGG	1 cut(s)	2646
Eco88I	CYCGRG	3 cut(s)	266, 683, 762
Eco91I	GGTNACC	1 cut(s)	1336
EcoICRI	GAGCTC	4 cut(s)	44, 1189, 1246, 1416
EcoO65I	GGTNACC	1 cut(s)	1336
EcoRII	CCWGG	2 cut(s)	750, 1117
EcoRV	GATATC	1 cut(s)	2587
EcoT14I	CCWWGG	3 cut(s)	231, 1192, 2118
EcoT38I	GRGCYC	4 cut(s)	46, 1191, 1248, 1418
ErhI	CCWWGG	3 cut(s)	231, 1192, 2118
Esp3I	CGTCTC	1 cut(s)	156
FalI	AAGNNNNNCTT	4 cut(s)	1365, 1397, 1913, 1945
FaqI	GGGAC	1 cut(s)	2532
FbaI	TGATCA	2 cut(s)	1878, 2437
FblI	GTMKAC	3 cut(s)	959, 1860, 1951
FokI	GGATG	1 cut(s)	2473
FriOI	GRGCYC	4 cut(s)	46, 1191, 1248, 1418
FspBI	CTAG	5 cut(s)	525, 1772, 1842, 2205, 2627
GlaI	GCGC	2 cut(s)	2085, 2429
GsuI	CTGGAG	1 cut(s)	734
HaeII	RGCGCY	1 cut(s)	2431
HaeIII	GGCC	4 cut(s)	941, 1467, 2117, 2701
HapII	CCGG	3 cut(s)	99, 763, 1487
HgaI	GACGC	1 cut(s)	79
HhaI	GCGC	2 cut(s)	2086, 2430
Hin6I	GCGC	2 cut(s)	2084, 2428
HinP1I	GCGC	2 cut(s)	2084, 2428
HincII	GTYRAC	2 cut(s)	960, 1379
HindII	GTYRAC	2 cut(s)	960, 1379
HindIII	AAGCTT	3 cut(s)	854, 864, 1922
HinfI	GANTC	7 cut(s)	157, 330, 742, 787, 2073, 2470, 2512
HpaII	CCGG	3 cut(s)	99, 763, 1487
HphI	GGTGA	4 cut(s)	823, 1808, 2521, 2561
Hpy166II	GTNNAC	7 cut(s)	960, 1010, 1379, 1811, 1861, 1952, 2402
Hpy188III	TCNNGA	5 cut(s)	737, 1670, 1876, 2249, 2455
Hpy8I	GTNNAC	7 cut(s)	960, 1010, 1379, 1811, 1861, 1952, 2402
Hpy99I	CGWCG	1 cut(s)	86
HpyCH4III	ACNGT	8 cut(s)	842, 928, 1033, 1126, 1873, 1978, 2414, 2528
HpyCH4IV	ACGT	5 cut(s)	30, 815, 1168, 1400, 1965
HpyF10VI	GCNNNNNNNGC	8 cut(s)	221, 386, 1709, 2090, 2205, 2333, 2399, 2427
HpySE526I	ACGT	5 cut(s)	30, 815, 1168, 1400, 1965
HspAI	GCGC	2 cut(s)	2084, 2428
Ksp22I	TGATCA	2 cut(s)	1878, 2437
Kzo9I	GATC	6 cut(s)	1353, 1739, 1878, 2014, 2344, 2437
LmnI	GCTCC	5 cut(s)	49, 180, 249, 1194, 2425
Lsp1109I	GCAGC	6 cut(s)	114, 122, 236, 887, 1512, 2261
LweI	GCATC	7 cut(s)	558, 1086, 1596, 1610, 1716, 2116, 2149
MaeI	CTAG	5 cut(s)	525, 1772, 1842, 2205, 2627
MaeII	ACGT	5 cut(s)	30, 815, 1168, 1400, 1965
MalI	GATC	6 cut(s)	1355, 1741, 1880, 2016, 2346, 2439
MboI	GATC	6 cut(s)	1353, 1739, 1878, 2014, 2344, 2437
MfeI	CAATTG	3 cut(s)	169, 1660, 2194
MhlI	GDGCHC	6 cut(s)	46, 1191, 1248, 1418, 1813, 2404
MlyI	GAGTC	2 cut(s)	751, 796
MmeI	TCCRAC	5 cut(s)	454, 510, 675, 1803, 1992
MroXI	GAANNNNTTC	3 cut(s)	867, 1923, 2077
MseI	TTAA	9 cut(s)	401, 413, 618, 1404, 1514, 1986, 1998, 2217, 2573
MslI	CAYNNNNRTG	2 cut(s)	795, 1874
MspI	CCGG	3 cut(s)	99, 763, 1487
MspR9I	CCNGG	5 cut(s)	752, 763, 764, 1119, 1488
MunI	CAATTG	3 cut(s)	169, 1660, 2194
Mva1269I	GAATGC	2 cut(s)	298, 1498
MvaI	CCWGG	2 cut(s)	752, 1119
MvnI	CGCG	1 cut(s)	144
MwoI	GCNNNNNNNGC	8 cut(s)	221, 386, 1709, 2090, 2205, 2333, 2399, 2427
NciI	CCSGG	3 cut(s)	763, 764, 1488
NdeII	GATC	6 cut(s)	1353, 1739, 1878, 2014, 2344, 2437
NlaIV	GGNNCC	2 cut(s)	216, 2643
NmeAIII	GCCGAG	2 cut(s)	131, 933
NmuCI	GTSAC	3 cut(s)	811, 947, 1756
NspI	RCATGY	3 cut(s)	1703, 1939, 2240
NspV	TTCGAA	2 cut(s)	57, 1231
OliI	CACNNNNGTG	1 cut(s)	1874
PaeI	GCATGC	1 cut(s)	1703
PagI	TCATGA	1 cut(s)	2454
PceI	AGGCCT	1 cut(s)	941
PctI	GAATGC	2 cut(s)	298, 1498
PdmI	GAANNNNTTC	3 cut(s)	867, 1923, 2077
PfeI	GAWTC	5 cut(s)	157, 330, 2073, 2470, 2512
PfoI	TCCNGGA	1 cut(s)	750
PleI	GAGTC	2 cut(s)	750, 795
PpsI	GAGTC	2 cut(s)	750, 795
PshBI	ATTAAT	1 cut(s)	618
PsiI	TTATAA	1 cut(s)	1272
Psp124BI	GAGCTC	4 cut(s)	46, 1191, 1248, 1418
Psp1406I	AACGTT	1 cut(s)	1400
Psp6I	CCWGG	2 cut(s)	750, 1117
PspEI	GGTNACC	1 cut(s)	1336
PspGI	CCWGG	2 cut(s)	750, 1117
PspN4I	GGNNCC	2 cut(s)	216, 2643
RsaI	GTAC	7 cut(s)	712, 818, 913, 919, 930, 2294, 2364
RsaNI	GTAC	7 cut(s)	711, 817, 912, 918, 929, 2293, 2363
RseI	CAYNNNNRTG	2 cut(s)	795, 1874
SacI	GAGCTC	4 cut(s)	46, 1191, 1248, 1418
SalI	GTCGAC	1 cut(s)	958
SaqAI	TTAA	9 cut(s)	401, 413, 618, 1404, 1514, 1986, 1998, 2217, 2573
Sau3AI	GATC	6 cut(s)	1353, 1739, 1878, 2014, 2344, 2437
ScaI	AGTACT	2 cut(s)	913, 2364
SchI	GAGTC	2 cut(s)	751, 796
ScrFI	CCNGG	5 cut(s)	752, 763, 764, 1119, 1488
SduI	GDGCHC	6 cut(s)	46, 1191, 1248, 1418, 1813, 2404
SfaNI	GCATC	7 cut(s)	558, 1086, 1596, 1610, 1716, 2116, 2149
SfcI	CTRYAG	2 cut(s)	604, 2154
SfuI	TTCGAA	2 cut(s)	57, 1231
SmaI	CCCGGG	1 cut(s)	764
SmiMI	CAYNNNNRTG	2 cut(s)	795, 1874
SmlI	CTYRAG	1 cut(s)	191
SmoI	CTYRAG	1 cut(s)	191
SpeI	ACTAGT	1 cut(s)	1771
SphI	GCATGC	1 cut(s)	1703
SseBI	AGGCCT	1 cut(s)	941
SsiI	CCGC	6 cut(s)	228, 252, 1703, 1846, 2373, 2691
SspI	AATATT	1 cut(s)	1348
SspMI	CTAG	5 cut(s)	525, 1772, 1842, 2205, 2627
SstI	GAGCTC	4 cut(s)	46, 1191, 1248, 1418
StuI	AGGCCT	1 cut(s)	941
StyD4I	CCNGG	5 cut(s)	750, 761, 762, 1117, 1486
StyI	CCWWGG	3 cut(s)	231, 1192, 2118
TaaI	ACNGT	8 cut(s)	842, 928, 1033, 1126, 1873, 1978, 2414, 2528
TaiI	ACGT	5 cut(s)	33, 818, 1171, 1403, 1968
TatI	WGTACW	5 cut(s)	710, 911, 928, 2292, 2362
TauI	GCSGC	5 cut(s)	230, 255, 1706, 1848, 2376
TfiI	GAWTC	5 cut(s)	157, 330, 2073, 2470, 2512
Tru1I	TTAA	9 cut(s)	401, 413, 618, 1404, 1514, 1986, 1998, 2217, 2573
Tru9I	TTAA	9 cut(s)	401, 413, 618, 1404, 1514, 1986, 1998, 2217, 2573
TscAI	CASTG	3 cut(s)	1146, 1456, 2533
TseFI	GTSAC	3 cut(s)	811, 947, 1756
TseI	GCWGC	6 cut(s)	102, 110, 224, 900, 1500, 2274
Tsp45I	GTSAC	3 cut(s)	811, 947, 1756
TspDTI	ATGAA	6 cut(s)	821, 876, 1010, 1557, 2124, 2443
TspGWI	ACGGA	3 cut(s)	70, 1914, 2479
TspMI	CCCGGG	1 cut(s)	762
TspRI	CASTG	3 cut(s)	1146, 1456, 2533
VneI	GTGCAC	2 cut(s)	1809, 2400
VspI	ATTAAT	1 cut(s)	618
XapI	RAATTY	1 cut(s)	343
XceI	RCATGY	3 cut(s)	1703, 1939, 2240
XmaI	CCCGGG	1 cut(s)	762
XmiI	GTMKAC	3 cut(s)	959, 1860, 1951
XmnI	GAANNNNTTC	3 cut(s)	867, 1923, 2077
XspI	CTAG	5 cut(s)	525, 1772, 1842, 2205, 2627
ZrmI	AGTACT	2 cut(s)	913, 2364

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.