MD10G1177500.v1.1

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0028208
Physical Location & Seq
Forward (+)
27164214 .. 27164810
597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1177500.v1.1.491

Sequence Viewer

Length: 597 bp
ATGGGGAGGACTGGATTGTTTGATCTTGAAAGGTACTTCACCATCTATGGAGCATATCACAGCAACCCAGTTAACGTTTTGCTACATACTTTCTTAGTGTGGCCCTTAGTCTTCGCTGTTGCGGTTCTTCTCCACTTCACACCTTCCCTATATAATTTCCCATCTGGGTTAAACAATCACGGTTTCGAATTGAATGTGGGGTTGGTTTTGACTTTGATGTATGCCATCTTTTATGTCAAACTGGACAAGAAAGCTGGGTCCTTGGCAGCTTTGTTATTGTTTCTCACCTGGGTTGGAGCCAATGTTCTTGCTAGTGGGCTAGGGTTTGGTAAGTCATGGAAGCTTGTTCTCGCTGTTCAGGTTTTCTGCTGGCCTGCACTGCTCATAGGCCATGGCGTTTTTGAGAAAAGATTACCAGGTATTGCAGACAGCTTTGTTGAAGGACTTCTGATGGGGCCGTACTTTGTGTTGCTTGAGTTTCTTCAATCAGCCTTTAGCTATGAACCGTATCCAGGGTTTAATAAAAGCGTGAAGGAAAAGATTGAAGCTGATATCAAAGCTTGGAAGGCCATCAAGAATCAAAAGAAACTCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

199

Amino Acids

22.13

Weight (kDa)

9.23

Isoelectric Point (pI)

21.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 8 - 162 4.6e-17 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028208)

Species Orthologous Gene IDs
malus_domestica MD10G1177500.v1.1
pyrus_communis pycom10g15470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 122
AclI AACGTT 1 cut(s) 75
AfaI GTAC 2 cut(s) 35, 461
AfiI CCNNNNNNNGG 1 cut(s) 512
AgsI TTSAA 5 cut(s) 29, 193, 440, 485, 545
AjnI CCWGG 3 cut(s) 287, 415, 511
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AloI GAACNNNNNNTCC 2 cut(s) 288, 320
AluBI AGCT 7 cut(s) 254, 269, 343, 432, 498, 548, 560
AluI AGCT 7 cut(s) 254, 269, 343, 432, 498, 548, 560
AoxI GGCC 5 cut(s) 101, 371, 388, 455, 567
ApeKI GCWGC 1 cut(s) 266
Asp700I GAANNNNTTC 1 cut(s) 444
AspS9I GGNCC 3 cut(s) 102, 258, 455
AsuHPI GGTGA 2 cut(s) 31, 277
AsuII TTCGAA 1 cut(s) 186
AvaII GGWCC 1 cut(s) 258
BbsI GAAGAC 1 cut(s) 103
BbvI GCAGC 1 cut(s) 278
BccI CCATC 5 cut(s) 50, 169, 233, 445, 578
BceAI ACGGC 1 cut(s) 442
BciT130I CCWGG 3 cut(s) 289, 417, 513
BciVI GTATCC 1 cut(s) 519
BfaI CTAG 2 cut(s) 312, 320
BfuI GTATCC 1 cut(s) 519
BisI GCNGC 1 cut(s) 267
BlsI GCNGC 1 cut(s) 268
Bme1390I CCNGG 3 cut(s) 289, 417, 513
Bme18I GGWCC 1 cut(s) 258
BmgT120I GGNCC 3 cut(s) 102, 258, 455
BmiI GGNNCC 3 cut(s) 259, 298, 456
BmrFI CCNGG 3 cut(s) 289, 417, 513
BmrI ACTGGG 1 cut(s) 62
BmuI ACTGGG 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 103
Bpu14I TTCGAA 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 494
BsaJI CCNNGG 4 cut(s) 261, 288, 391, 512
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 1 cut(s) 512
Bse1I ACTGG 3 cut(s) 16, 68, 246
BseBI CCWGG 3 cut(s) 289, 417, 513
BseDI CCNNGG 4 cut(s) 261, 288, 391, 512
BseLI CCNNNNNNNGG 1 cut(s) 512
BseNI ACTGG 3 cut(s) 16, 68, 246
BseXI GCAGC 1 cut(s) 278
BseYI CCCAGC 1 cut(s) 254
BsgI GTGCAG 1 cut(s) 360
BshFI GGCC 5 cut(s) 103, 373, 390, 457, 569
BslI CCNNNNNNNGG 1 cut(s) 512
BsnI GGCC 5 cut(s) 103, 373, 390, 457, 569
Bsp119I TTCGAA 1 cut(s) 186
Bsp143I GATC 1 cut(s) 22
Bsp19I CCATGG 1 cut(s) 391
BspACI CCGC 1 cut(s) 122
BspANI GGCC 5 cut(s) 103, 373, 390, 457, 569
BspLI GGNNCC 3 cut(s) 259, 298, 456
BspT104I TTCGAA 1 cut(s) 186
BsrI ACTGG 3 cut(s) 16, 68, 246
BssECI CCNNGG 4 cut(s) 261, 288, 391, 512
BssMI GATC 1 cut(s) 22
BssT1I CCWWGG 2 cut(s) 261, 391
Bst2UI CCWGG 3 cut(s) 289, 417, 513
Bst4CI ACNGT 2 cut(s) 182, 507
BstBI TTCGAA 1 cut(s) 186
BstC8I GCNNGC 2 cut(s) 371, 375
BstDEI CTNAG 2 cut(s) 94, 106
BstDSI CCRYGG 1 cut(s) 391
BstKTI GATC 1 cut(s) 25
BstMBI GATC 1 cut(s) 22
BstMWI GCNNNNNNNGC 2 cut(s) 379, 566
BstNI CCWGG 3 cut(s) 289, 417, 513
BstSCI CCNGG 3 cut(s) 287, 415, 511
BstV1I GCAGC 1 cut(s) 278
BstV2I GAAGAC 1 cut(s) 103
BsuI GTATCC 1 cut(s) 519
BsuRI GGCC 5 cut(s) 103, 373, 390, 457, 569
BtgI CCRYGG 1 cut(s) 391
BtsI GCAGTG 1 cut(s) 377
BtsIMutI CAGTG 1 cut(s) 377
Cac8I GCNNGC 2 cut(s) 371, 375
Cfr13I GGNCC 3 cut(s) 102, 258, 455
CsiI ACCWGGT 1 cut(s) 415
Csp6I GTAC 2 cut(s) 34, 460
CviAII CATG 2 cut(s) 336, 392
CviQI GTAC 2 cut(s) 34, 460
DdeI CTNAG 2 cut(s) 94, 106
DpnI GATC 1 cut(s) 24
DpnII GATC 1 cut(s) 22
Eco130I CCWWGG 2 cut(s) 261, 391
Eco32I GATATC 1 cut(s) 553
Eco47I GGWCC 1 cut(s) 258
EcoO109I RGGNCCY 1 cut(s) 258
EcoRII CCWGG 3 cut(s) 287, 415, 511
EcoRV GATATC 1 cut(s) 553
EcoT14I CCWWGG 2 cut(s) 261, 391
ErhI CCWWGG 2 cut(s) 261, 391
FaeI CATG 2 cut(s) 339, 395
FatI CATG 2 cut(s) 335, 391
Fnu4HI GCNGC 1 cut(s) 267
Fsp4HI GCNGC 1 cut(s) 267
FspBI CTAG 2 cut(s) 312, 320
GluI GCNGC 1 cut(s) 267
GsaI CCCAGC 1 cut(s) 258
HaeIII GGCC 5 cut(s) 103, 373, 390, 457, 569
Hin1II CATG 2 cut(s) 339, 395
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HindIII AAGCTT 2 cut(s) 341, 558
HinfI GANTC 1 cut(s) 577
HpaI GTTAAC 1 cut(s) 73
HphI GGTGA 2 cut(s) 31, 277
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 1 cut(s) 450
Hpy188III TCNNGA 3 cut(s) 26, 574, 594
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 4 cut(s) 153, 434, 526, 559
HpyCH4III ACNGT 2 cut(s) 182, 507
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 2 cut(s) 377, 425
HpyF10VI GCNNNNNNNGC 2 cut(s) 379, 566
HpyF3I CTNAG 2 cut(s) 94, 106
HpySE526I ACGT 1 cut(s) 75
Hsp92II CATG 2 cut(s) 339, 395
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 1 cut(s) 22
LmnI GCTCC 2 cut(s) 50, 296
Lsp1109I GCAGC 1 cut(s) 278
MabI ACCWGGT 1 cut(s) 415
MaeI CTAG 2 cut(s) 312, 320
MaeII ACGT 1 cut(s) 75
MalI GATC 1 cut(s) 24
MboI GATC 1 cut(s) 22
MboII GAAGA 3 cut(s) 103, 119, 473
MluCI AATT 2 cut(s) 154, 188
MmeI TCCRAC 1 cut(s) 274
MroXI GAANNNNTTC 1 cut(s) 444
MseI TTAA 3 cut(s) 72, 170, 519
MspR9I CCNGG 3 cut(s) 289, 417, 513
MvaI CCWGG 3 cut(s) 289, 417, 513
MwoI GCNNNNNNNGC 2 cut(s) 379, 566
NcoI CCATGG 1 cut(s) 391
NdeII GATC 1 cut(s) 22
NlaIII CATG 2 cut(s) 339, 395
NlaIV GGNNCC 3 cut(s) 259, 298, 456
NspV TTCGAA 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 1 cut(s) 577
PkrI GCNGC 1 cut(s) 268
PpuMI RGGWCCY 1 cut(s) 258
Psp1406I AACGTT 1 cut(s) 75
Psp5II RGGWCCY 1 cut(s) 258
Psp6I CCWGG 3 cut(s) 287, 415, 511
PspFI CCCAGC 1 cut(s) 254
PspGI CCWGG 3 cut(s) 287, 415, 511
PspN4I GGNNCC 3 cut(s) 259, 298, 456
PspPI GGNCC 3 cut(s) 102, 258, 455
PspPPI RGGWCCY 1 cut(s) 258
RsaI GTAC 2 cut(s) 35, 461
RsaNI GTAC 2 cut(s) 34, 460
SaqAI TTAA 3 cut(s) 72, 170, 519
SatI GCNGC 1 cut(s) 267
Sau3AI GATC 1 cut(s) 22
Sau96I GGNCC 3 cut(s) 102, 258, 455
ScrFI CCNGG 3 cut(s) 289, 417, 513
SexAI ACCWGGT 1 cut(s) 415
SfuI TTCGAA 1 cut(s) 186
SinI GGWCC 1 cut(s) 258
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
Sse9I AATT 2 cut(s) 154, 188
SsiI CCGC 1 cut(s) 122
SspMI CTAG 2 cut(s) 312, 320
StyD4I CCNGG 3 cut(s) 287, 415, 511
StyI CCWWGG 2 cut(s) 261, 391
TaaI ACNGT 2 cut(s) 182, 507
TaiI ACGT 1 cut(s) 78
TaqI TCGA 1 cut(s) 186
TasI AATT 2 cut(s) 154, 188
TfiI GAWTC 1 cut(s) 577
Tru1I TTAA 3 cut(s) 72, 170, 519
Tru9I TTAA 3 cut(s) 72, 170, 519
TscAI CASTG 1 cut(s) 384
TseI GCWGC 1 cut(s) 266
TspDTI ATGAA 1 cut(s) 516
TspRI CASTG 1 cut(s) 384
VpaK11BI GGWCC 1 cut(s) 258
XmnI GAANNNNTTC 1 cut(s) 444
XspI CTAG 2 cut(s) 312, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.