pycom10g15470

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0028208
Physical Location & Seq
Forward (+)
18718135 .. 18718728
594 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g15470.1

Sequence Viewer

Length: 594 bp
ATGGGGAGGACTGGATTGTTTGATCTTGAAAGACACTTCGCCTTCTATAGAGCATATCACAGCAACCCGGTTAACGTTTTGCTACACACTTTCTTAGTGTGGCCATTAATCTTCACTGTTGATGTTCTTCTCCACTTCACACCACCTCTCTATAATTTCCCATCTGGGTTAAACTATCACGGTTTCGAATTGAATGTTGGGTTGGTTTTGACTTTGATTTATGCTATCTTTTGTGTCAAATTGGACAAGAAAGCTGGGTCCTTGGCAGGTTTGCTATTGTTTCTCACCAGGGTTGGAGCCAGTGTTCTTGCTAGTGGCCTAGGGTTTGCTAAGTCATGGAAGCTTGTTCTTGCTGTTCAGGCTTTCTGCTGGCCTGCACTGCTCATAGGCCATGGTGTTTTTGAGAAACGAATACCAGGCCTTGCAGACGGCTTTTTTGAAGGATTTATGGAGCCGTACTATGTGTTGCTTGAGTTTCTTCAATCAGCCTTTGGCTATGAACCCTATCCAGGGTTTAATAAGAGCGTGAAAGAAAAGATTGAAGCTGATATCAAATCGTGGAAGGTGATCAAGAATCAAAAGAAACTCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

198

Amino Acids

22.23

Weight (kDa)

9.15

Isoelectric Point (pI)

21.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 8 - 160 2.7e-15 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028208)

Species Orthologous Gene IDs
malus_domestica MD10G1177500.v1.1
pyrus_communis pycom10g15470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 257
AclI AACGTT 1 cut(s) 75
AcoI YGGCCR 1 cut(s) 101
AfaI GTAC 1 cut(s) 458
AfiI CCNNNNNNNGG 2 cut(s) 509, 510
AgsI TTSAA 5 cut(s) 29, 193, 440, 482, 542
AjnI CCWGG 3 cut(s) 287, 415, 508
AjuI GAANNNNNNNTTGG 4 cut(s) 180, 185, 212, 217
AloI GAACNNNNNNTCC 2 cut(s) 288, 320
AluBI AGCT 3 cut(s) 254, 343, 545
AluI AGCT 3 cut(s) 254, 343, 545
AoxI GGCC 5 cut(s) 101, 316, 371, 388, 418
ArsI GACNNNNNNTTYG 2 cut(s) 419, 451
AseI ATTAAT 1 cut(s) 107
AspA2I CCTAGG 1 cut(s) 319
AspS9I GGNCC 1 cut(s) 258
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 2 cut(s) 277, 577
AsuII TTCGAA 1 cut(s) 186
AvaII GGWCC 1 cut(s) 258
AvrII CCTAGG 1 cut(s) 319
BalI TGGCCA 1 cut(s) 103
BccI CCATC 1 cut(s) 169
BceAI ACGGC 2 cut(s) 439, 445
BciT130I CCWGG 3 cut(s) 289, 417, 510
BclI TGATCA 1 cut(s) 567
BcnI CCSGG 1 cut(s) 68
BfaI CTAG 2 cut(s) 312, 320
BfmI CTRYAG 1 cut(s) 46
BfuAI ACCTGC 1 cut(s) 257
BlnI CCTAGG 1 cut(s) 319
Bme1390I CCNGG 4 cut(s) 68, 289, 417, 510
Bme18I GGWCC 1 cut(s) 258
BmgT120I GGNCC 1 cut(s) 258
BmiI GGNNCC 3 cut(s) 259, 298, 453
BmrFI CCNGG 4 cut(s) 68, 289, 417, 510
Bpu14I TTCGAA 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 491
BpuMI CCSGG 1 cut(s) 68
BsaJI CCNNGG 5 cut(s) 261, 288, 319, 391, 509
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 2 cut(s) 509, 510
Bse1I ACTGG 2 cut(s) 16, 300
BseBI CCWGG 3 cut(s) 289, 417, 510
BseDI CCNNGG 5 cut(s) 261, 288, 319, 391, 509
BseLI CCNNNNNNNGG 2 cut(s) 509, 510
BseNI ACTGG 2 cut(s) 16, 300
BseYI CCCAGC 1 cut(s) 254
BsgI GTGCAG 1 cut(s) 360
BshFI GGCC 5 cut(s) 103, 318, 373, 390, 420
BsiSI CCGG 1 cut(s) 68
BslI CCNNNNNNNGG 2 cut(s) 509, 510
BsnI GGCC 5 cut(s) 103, 318, 373, 390, 420
Bsp119I TTCGAA 1 cut(s) 186
Bsp143I GATC 2 cut(s) 22, 567
Bsp19I CCATGG 1 cut(s) 391
BspANI GGCC 5 cut(s) 103, 318, 373, 390, 420
BspLI GGNNCC 3 cut(s) 259, 298, 453
BspMI ACCTGC 1 cut(s) 257
BspT104I TTCGAA 1 cut(s) 186
BsrI ACTGG 2 cut(s) 16, 300
BssECI CCNNGG 5 cut(s) 261, 288, 319, 391, 509
BssMI GATC 2 cut(s) 22, 567
BssT1I CCWWGG 3 cut(s) 261, 319, 391
Bst2UI CCWGG 3 cut(s) 289, 417, 510
Bst4CI ACNGT 2 cut(s) 118, 182
BstBI TTCGAA 1 cut(s) 186
BstC8I GCNNGC 2 cut(s) 371, 375
BstDEI CTNAG 2 cut(s) 94, 330
BstDSI CCRYGG 1 cut(s) 391
BstKTI GATC 2 cut(s) 25, 570
BstMBI GATC 2 cut(s) 22, 567
BstMWI GCNNNNNNNGC 2 cut(s) 359, 379
BstNI CCWGG 3 cut(s) 289, 417, 510
BstSCI CCNGG 4 cut(s) 66, 287, 415, 508
BstSFI CTRYAG 1 cut(s) 46
BsuRI GGCC 5 cut(s) 103, 318, 373, 390, 420
BtgI CCRYGG 1 cut(s) 391
BtsI GCAGTG 1 cut(s) 377
BtsIMutI CAGTG 3 cut(s) 114, 307, 377
BveI ACCTGC 1 cut(s) 257
Cac8I GCNNGC 2 cut(s) 371, 375
Cfr13I GGNCC 1 cut(s) 258
Csp6I GTAC 1 cut(s) 457
CviAII CATG 2 cut(s) 336, 392
CviQI GTAC 1 cut(s) 457
DdeI CTNAG 2 cut(s) 94, 330
DpnI GATC 2 cut(s) 24, 569
DpnII GATC 2 cut(s) 22, 567
EaeI YGGCCR 1 cut(s) 101
Eco130I CCWWGG 3 cut(s) 261, 319, 391
Eco147I AGGCCT 1 cut(s) 420
Eco32I GATATC 1 cut(s) 550
Eco47I GGWCC 1 cut(s) 258
EcoO109I RGGNCCY 1 cut(s) 258
EcoRII CCWGG 3 cut(s) 287, 415, 508
EcoRV GATATC 1 cut(s) 550
EcoT14I CCWWGG 3 cut(s) 261, 319, 391
ErhI CCWWGG 3 cut(s) 261, 319, 391
FaeI CATG 2 cut(s) 339, 395
FatI CATG 2 cut(s) 335, 391
FbaI TGATCA 1 cut(s) 567
FspBI CTAG 2 cut(s) 312, 320
GsaI CCCAGC 1 cut(s) 258
HaeIII GGCC 5 cut(s) 103, 318, 373, 390, 420
HapII CCGG 1 cut(s) 68
Hin1II CATG 2 cut(s) 339, 395
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 1 cut(s) 574
HpaI GTTAAC 1 cut(s) 73
HpaII CCGG 1 cut(s) 68
HphI GGTGA 2 cut(s) 277, 577
Hpy166II GTNNAC 1 cut(s) 73
Hpy188III TCNNGA 3 cut(s) 26, 571, 591
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 3 cut(s) 52, 434, 556
HpyCH4III ACNGT 2 cut(s) 118, 182
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 2 cut(s) 377, 425
HpyF10VI GCNNNNNNNGC 2 cut(s) 359, 379
HpyF3I CTNAG 2 cut(s) 94, 330
HpySE526I ACGT 1 cut(s) 75
Hsp92II CATG 2 cut(s) 339, 395
Ksp22I TGATCA 1 cut(s) 567
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 2 cut(s) 22, 567
LmnI GCTCC 2 cut(s) 296, 451
MaeI CTAG 2 cut(s) 312, 320
MaeII ACGT 1 cut(s) 75
MalI GATC 2 cut(s) 24, 569
MboI GATC 2 cut(s) 22, 567
MboII GAAGA 3 cut(s) 103, 119, 470
MlsI TGGCCA 1 cut(s) 103
MluCI AATT 3 cut(s) 154, 188, 239
MluNI TGGCCA 1 cut(s) 103
MmeI TCCRAC 1 cut(s) 274
MnlI CCTC 1 cut(s) 156
Mox20I TGGCCA 1 cut(s) 103
MscI TGGCCA 1 cut(s) 103
MseI TTAA 4 cut(s) 72, 107, 170, 516
Msp20I TGGCCA 1 cut(s) 103
MspI CCGG 1 cut(s) 68
MspR9I CCNGG 4 cut(s) 68, 289, 417, 510
MvaI CCWGG 3 cut(s) 289, 417, 510
MwoI GCNNNNNNNGC 2 cut(s) 359, 379
NciI CCSGG 1 cut(s) 68
NcoI CCATGG 1 cut(s) 391
NdeII GATC 2 cut(s) 22, 567
NlaIII CATG 2 cut(s) 339, 395
NlaIV GGNNCC 3 cut(s) 259, 298, 453
NspV TTCGAA 1 cut(s) 186
PceI AGGCCT 1 cut(s) 420
PfeI GAWTC 1 cut(s) 574
PpuMI RGGWCCY 1 cut(s) 258
PshBI ATTAAT 1 cut(s) 107
Psp1406I AACGTT 1 cut(s) 75
Psp5II RGGWCCY 1 cut(s) 258
Psp6I CCWGG 3 cut(s) 287, 415, 508
PspFI CCCAGC 1 cut(s) 254
PspGI CCWGG 3 cut(s) 287, 415, 508
PspN4I GGNNCC 3 cut(s) 259, 298, 453
PspPI GGNCC 1 cut(s) 258
PspPPI RGGWCCY 1 cut(s) 258
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
SaqAI TTAA 4 cut(s) 72, 107, 170, 516
Sau3AI GATC 2 cut(s) 22, 567
Sau96I GGNCC 1 cut(s) 258
ScrFI CCNGG 4 cut(s) 68, 289, 417, 510
SetI ASST 7 cut(s) 78, 148, 256, 271, 345, 547, 567
SfcI CTRYAG 1 cut(s) 46
SfuI TTCGAA 1 cut(s) 186
SinI GGWCC 1 cut(s) 258
SmlI CTYRAG 1 cut(s) 470
SmoI CTYRAG 1 cut(s) 470
Sse9I AATT 3 cut(s) 154, 188, 239
SseBI AGGCCT 1 cut(s) 420
SspMI CTAG 2 cut(s) 312, 320
StuI AGGCCT 1 cut(s) 420
StyD4I CCNGG 4 cut(s) 66, 287, 415, 508
StyI CCWWGG 3 cut(s) 261, 319, 391
TaaI ACNGT 2 cut(s) 118, 182
TaiI ACGT 1 cut(s) 78
TaqI TCGA 1 cut(s) 186
TasI AATT 3 cut(s) 154, 188, 239
TfiI GAWTC 1 cut(s) 574
Tru1I TTAA 4 cut(s) 72, 107, 170, 516
Tru9I TTAA 4 cut(s) 72, 107, 170, 516
TscAI CASTG 3 cut(s) 121, 307, 384
TspDTI ATGAA 1 cut(s) 513
TspRI CASTG 3 cut(s) 121, 307, 384
VpaK11BI GGWCC 1 cut(s) 258
VspI ATTAAT 1 cut(s) 107
XmaJI CCTAGG 1 cut(s) 319
XspI CTAG 2 cut(s) 312, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.