MD10G1242800.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0024108
Physical Location & Seq
Forward (+)
33758392 .. 33760857
2466 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1242800.v1.1.491

Sequence Viewer

Length: 1119 bp
ATGGATTCTGCGAGGAGTTGGCTTCAGAAGTTCCAGCCGAGGGTGAAGAAGAAGGCGGCGGCTGAGAATTCGAAAGACGAGCAGCAGAATTCTGCTTCCGATGAGGCGCCGTCGAGCGTGACGAAACAGAAGGTGGAGGCGGCCAAACAGTACATTGAGAACCATTACAAGGCACAAATGAAGTCCTTGGAGGATAGGAAGGAGAGGCGGTGGATGCTCGAAAGGCGGCTGGCGGATGCGGATGTTTCGCAGGAGGATCAGATGAATGTGTTGAAGTATTTGGAGCAGAAGGAGACGGAGTATATGCACCTTCAGAGGCATAAAATGGGGGTTGATGACTTTCAGCTTTTGACTATTATCGGAAGGGGTGCATTCGGAGAGGTCAGAATTTGTAGAGAAAAAACTACTGGGCATGTATATGCCATGAAAAAGCTTAAGAAATCGGAGATGCTCCGGAGAGGCCAGGTGGAACATGTTAAAGCTGAAAGAAATCTTCTTGCTGAGGTTGATAGTGCTTACATCGTTAAGCTCTACTGCTCCTTTCAAGACGAAGAATTTTTGTATCTTATAATGGAGTATCTTCCGGGGGGTGATATGATGACATTACTAATGCGCAAAGATATCTTGACTGAAGACGAAGCCAGGTTTTACGTTGGAGAGACGGTCCTTGCCATTGAATCTATTCACAAGCACAACTACATCCACAGGGATATTAAGCCTGATAATTTATTGCTCGACTCTCACGGCCACATGAAGCTTTCAGATTTTGGGTTGTGTAAGCCTTTGGGCTCTAATAGCTTTCCAGATCTTAGTGAGAATGACAACGGGGTTGGAAGGAATTCAAATTCCCTTTCAGCGAGTCATAAGCATTCTAACCTTCCCACAACACCAAAACGGACCCAGCAGGAAAAGTTATTGCACTGGCAAAAGAACAGGCGAATGCTGGCTTATTCAACGGTTGGAACTCCAGATTACATTGCTCCGGAAGTATTGCTGAAGAAAGGATATGGAATGGAATGTGACTGGTGGTCTCTTGGTGCAATCATGTATGAGATGCTTGTAGGATTTCCACCTTTCTATTCCGAAGAACCAATGTCAACATGCAGAAAGGCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

373

Amino Acids

43.1

Weight (kDa)

7.67

Isoelectric Point (pI)

49.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 115 - 266 1.4e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 116 - 262 4.6e-22 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 166 - 264 3.1e-06 ABC1 atypical kinase-like domain
Pkinase PF00069 316 - 365 7.2e-14 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024108)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g03790
malus_domestica MD10G1242800.v1.1
pyrus_communis pycom05g24900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 569
Acc16I TGCGCA 1 cut(s) 614
AccB1I GGYRCC 1 cut(s) 106
AccIII TCCGGA 2 cut(s) 453, 982
AciI CCGC 7 cut(s) 56, 59, 140, 208, 226, 233, 239
AclWI GGATC 1 cut(s) 264
AcoI YGGCCR 2 cut(s) 141, 745
AcsI RAATTY 6 cut(s) 67, 88, 387, 554, 838, 844
AcuI CTGAAG 4 cut(s) 8, 296, 651, 1016
AcyI GRCGYC 1 cut(s) 107
AfaI GTAC 1 cut(s) 152
AfiI CCNNNNNNNGG 2 cut(s) 40, 169
AflII CTTAAG 1 cut(s) 434
AflIII ACRYGT 1 cut(s) 472
AgsI TTSAA 5 cut(s) 274, 545, 677, 843, 954
AhdI GACNNNNNGTC 1 cut(s) 1027
AjnI CCWGG 2 cut(s) 462, 641
AluBI AGCT 6 cut(s) 346, 433, 482, 529, 757, 798
AluI AGCT 6 cut(s) 346, 433, 482, 529, 757, 798
Alw26I GTCTC 3 cut(s) 287, 653, 1035
AlwI GGATC 1 cut(s) 264
Aor13HI TCCGGA 2 cut(s) 453, 982
AoxI GGCC 3 cut(s) 141, 460, 745
ApeKI GCWGC 1 cut(s) 82
ApoI RAATTY 6 cut(s) 67, 88, 387, 554, 838, 844
Asp700I GAANNNNTTC 2 cut(s) 681, 838
AspLEI GCGC 2 cut(s) 109, 615
AspS9I GGNCC 2 cut(s) 664, 897
AsuC2I CCSGG 1 cut(s) 585
AsuHPI GGTGA 2 cut(s) 55, 602
AsuII TTCGAA 1 cut(s) 71
AvaII GGWCC 2 cut(s) 664, 897
BanI GGYRCC 1 cut(s) 106
BanII GRGCYC 1 cut(s) 791
BbsI GAAGAC 1 cut(s) 639
BbvCI CCTCAGC 1 cut(s) 501
BbvI GCAGC 1 cut(s) 94
BceAI ACGGC 2 cut(s) 94, 760
BciT130I CCWGG 2 cut(s) 464, 643
BcnI CCSGG 1 cut(s) 585
BcoDI GTCTC 3 cut(s) 287, 653, 1035
BfoI RGCGCY 1 cut(s) 110
BfrI CTTAAG 1 cut(s) 434
BglII AGATCT 1 cut(s) 805
BisI GCNGC 5 cut(s) 57, 60, 83, 141, 227
BlsI GCNGC 5 cut(s) 58, 61, 84, 142, 228
Bme1390I CCNGG 3 cut(s) 464, 585, 643
Bme18I GGWCC 2 cut(s) 664, 897
BmeRI GACNNNNNGTC 1 cut(s) 1027
BmgT120I GGNCC 2 cut(s) 664, 897
BmiI GGNNCC 2 cut(s) 108, 899
BmrFI CCNGG 3 cut(s) 464, 585, 643
BmrI ACTGGG 1 cut(s) 417
BmsI GCATC 4 cut(s) 204, 226, 438, 1044
BmuI ACTGGG 1 cut(s) 417
BpiI GAAGAC 1 cut(s) 639
BpmI CTGGAG 1 cut(s) 951
Bpu10I CCTNAGC 1 cut(s) 501
Bpu14I TTCGAA 1 cut(s) 71
BpuMI CCSGG 1 cut(s) 585
BsaHI GRCGYC 1 cut(s) 107
BsaI GGTCTC 1 cut(s) 1035
BsaJI CCNNGG 3 cut(s) 38, 186, 584
BsaWI WCCGGW 2 cut(s) 453, 982
BsaXI ACNNNNNCTCC 6 cut(s) 194, 224, 284, 314, 648, 678
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 169
Bse1I ACTGG 3 cut(s) 412, 926, 1028
Bse3DI GCAATG 1 cut(s) 975
BseAI TCCGGA 2 cut(s) 453, 982
BseBI CCWGG 2 cut(s) 464, 643
BseDI CCNNGG 3 cut(s) 38, 186, 584
BseGI GGATG 4 cut(s) 219, 241, 247, 699
BseLI CCNNNNNNNGG 2 cut(s) 40, 169
BseMI GCAATG 1 cut(s) 975
BseMII CTCAG 2 cut(s) 54, 492
BseNI ACTGG 3 cut(s) 412, 926, 1028
BseRI GAGGAG 1 cut(s) 28
BseXI GCAGC 1 cut(s) 94
BseYI CCCAGC 1 cut(s) 900
BshFI GGCC 3 cut(s) 143, 462, 747
BshNI GGYRCC 1 cut(s) 106
BsiSI CCGG 3 cut(s) 454, 584, 983
BslI CCNNNNNNNGG 2 cut(s) 40, 169
BsmAI GTCTC 3 cut(s) 287, 653, 1035
BsmBI CGTCTC 2 cut(s) 287, 653
BsmI GAATGC 3 cut(s) 371, 868, 945
BsnI GGCC 3 cut(s) 143, 462, 747
Bso31I GGTCTC 1 cut(s) 1035
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 791
Bsp13I TCCGGA 2 cut(s) 453, 982
Bsp143I GATC 2 cut(s) 256, 805
BspACI CCGC 7 cut(s) 56, 59, 140, 208, 226, 233, 239
BspANI GGCC 3 cut(s) 143, 462, 747
BspCNI CTCAG 2 cut(s) 55, 493
BspEI TCCGGA 2 cut(s) 453, 982
BspLI GGNNCC 2 cut(s) 108, 899
BspPI GGATC 1 cut(s) 264
BspT104I TTCGAA 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 106
BspTI CTTAAG 1 cut(s) 434
BspTNI GGTCTC 1 cut(s) 1035
BsrDI GCAATG 1 cut(s) 975
BsrI ACTGG 3 cut(s) 412, 926, 1028
BssECI CCNNGG 3 cut(s) 38, 186, 584
BssMI GATC 2 cut(s) 256, 805
BssNI GRCGYC 1 cut(s) 107
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 464, 643
Bst4CI ACNGT 3 cut(s) 150, 664, 958
BstACI GRCGYC 1 cut(s) 107
BstAFI CTTAAG 1 cut(s) 434
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 2 cut(s) 231, 945
BstDEI CTNAG 3 cut(s) 63, 501, 809
BstF5I GGATG 4 cut(s) 219, 241, 247, 699
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 2 cut(s) 109, 615
BstKTI GATC 2 cut(s) 259, 808
BstMAI GTCTC 3 cut(s) 287, 653, 1035
BstMBI GATC 2 cut(s) 256, 805
BstMWI GCNNNNNNNGC 3 cut(s) 214, 223, 795
BstNI CCWGG 2 cut(s) 464, 643
BstNSI RCATGY 3 cut(s) 416, 476, 1104
BstSCI CCNGG 3 cut(s) 462, 583, 641
BstV1I GCAGC 1 cut(s) 94
BstV2I GAAGAC 1 cut(s) 639
BstX2I RGATCY 1 cut(s) 805
BstYI RGATCY 1 cut(s) 805
BsuRI GGCC 3 cut(s) 143, 462, 747
BtsCI GGATG 4 cut(s) 219, 241, 247, 699
BtsIMutI CAGTG 1 cut(s) 919
Cac8I GCNNGC 2 cut(s) 231, 945
CfoI GCGC 2 cut(s) 109, 615
Cfr13I GGNCC 2 cut(s) 664, 897
Csp6I GTAC 1 cut(s) 151
CviAII CATG 6 cut(s) 413, 424, 473, 751, 1045, 1101
CviQI GTAC 1 cut(s) 151
DdeI CTNAG 3 cut(s) 63, 501, 809
DinI GGCGCC 1 cut(s) 108
DpnI GATC 2 cut(s) 258, 807
DpnII GATC 2 cut(s) 256, 805
DriI GACNNNNNGTC 1 cut(s) 1027
EaeI YGGCCR 2 cut(s) 141, 745
Eam1105I GACNNNNNGTC 1 cut(s) 1027
EciI GGCGGA 1 cut(s) 248
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 791
Eco31I GGTCTC 1 cut(s) 1035
Eco32I GATATC 1 cut(s) 622
Eco47I GGWCC 2 cut(s) 664, 897
Eco57I CTGAAG 4 cut(s) 8, 296, 651, 1016
EcoRI GAATTC 3 cut(s) 67, 88, 838
EcoRII CCWGG 2 cut(s) 462, 641
EcoRV GATATC 1 cut(s) 622
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 791
EgeI GGCGCC 1 cut(s) 108
EheI GGCGCC 1 cut(s) 108
ErhI CCWWGG 1 cut(s) 186
Esp3I CGTCTC 2 cut(s) 287, 653
FaeI CATG 6 cut(s) 416, 427, 476, 754, 1048, 1104
FatI CATG 6 cut(s) 412, 423, 472, 750, 1044, 1100
Fnu4HI GCNGC 5 cut(s) 57, 60, 83, 141, 227
FokI GGATG 4 cut(s) 226, 248, 254, 686
FriOI GRGCYC 1 cut(s) 791
Fsp4HI GCNGC 5 cut(s) 57, 60, 83, 141, 227
FspI TGCGCA 1 cut(s) 614
GlaI GCGC 2 cut(s) 108, 614
GluI GCNGC 5 cut(s) 57, 60, 83, 141, 227
GsaI CCCAGC 1 cut(s) 904
GsuI CTGGAG 1 cut(s) 951
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 3 cut(s) 143, 462, 747
HapII CCGG 3 cut(s) 454, 584, 983
HhaI GCGC 2 cut(s) 109, 615
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 6 cut(s) 416, 427, 476, 754, 1048, 1104
Hin6I GCGC 2 cut(s) 107, 613
HinP1I GCGC 2 cut(s) 107, 613
HincII GTYRAC 1 cut(s) 1098
HindII GTYRAC 1 cut(s) 1098
HindIII AAGCTT 2 cut(s) 431, 755
HinfI GANTC 4 cut(s) 5, 677, 737, 859
HpaII CCGG 3 cut(s) 454, 584, 983
HphI GGTGA 2 cut(s) 55, 602
Hpy166II GTNNAC 1 cut(s) 1098
Hpy188III TCNNGA 6 cut(s) 454, 545, 625, 803, 968, 983
Hpy8I GTNNAC 1 cut(s) 1098
Hpy99I CGWCG 1 cut(s) 115
HpyAV CCTTC 8 cut(s) 46, 124, 193, 283, 320, 357, 828, 887
HpyCH4III ACNGT 3 cut(s) 150, 664, 958
HpyCH4IV ACGT 1 cut(s) 651
HpyCH4V TGCA 5 cut(s) 307, 371, 919, 1040, 1104
HpyF10VI GCNNNNNNNGC 3 cut(s) 214, 223, 795
HpyF3I CTNAG 3 cut(s) 63, 501, 809
HpySE526I ACGT 1 cut(s) 651
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 6 cut(s) 416, 427, 476, 754, 1048, 1104
HspAI GCGC 2 cut(s) 107, 613
KasI GGCGCC 1 cut(s) 106
Kpn2I TCCGGA 2 cut(s) 453, 982
Kzo9I GATC 2 cut(s) 256, 805
LmnI GCTCC 4 cut(s) 283, 456, 542, 985
Lsp1109I GCAGC 1 cut(s) 94
LweI GCATC 4 cut(s) 204, 226, 438, 1044
MaeII ACGT 1 cut(s) 651
MaeIII GTNAC 2 cut(s) 118, 1019
MalI GATC 2 cut(s) 258, 807
MboI GATC 2 cut(s) 256, 805
MboII GAAGA 8 cut(s) 58, 61, 485, 563, 572, 644, 1009, 1097
MflI RGATCY 1 cut(s) 805
MhlI GDGCHC 1 cut(s) 791
MluCI AATT 7 cut(s) 67, 88, 387, 554, 724, 838, 844
Mly113I GGCGCC 1 cut(s) 107
MlyI GAGTC 2 cut(s) 731, 868
MmeI TCCRAC 3 cut(s) 634, 811, 940
MroI TCCGGA 2 cut(s) 453, 982
MroXI GAANNNNTTC 2 cut(s) 681, 838
MseI TTAA 4 cut(s) 435, 477, 525, 714
MslI CAYNNNNRTG 1 cut(s) 417
MspCI CTTAAG 1 cut(s) 434
MspI CCGG 3 cut(s) 454, 584, 983
MspR9I CCNGG 3 cut(s) 464, 585, 643
Mva1269I GAATGC 3 cut(s) 371, 868, 945
MvaI CCWGG 2 cut(s) 464, 643
MwoI GCNNNNNNNGC 3 cut(s) 214, 223, 795
NarI GGCGCC 1 cut(s) 107
NciI CCSGG 1 cut(s) 585
NdeII GATC 2 cut(s) 256, 805
NlaIII CATG 6 cut(s) 416, 427, 476, 754, 1048, 1104
NlaIV GGNNCC 2 cut(s) 108, 899
NmeAIII GCCGAG 1 cut(s) 63
NmuCI GTSAC 2 cut(s) 118, 1019
NsbI TGCGCA 1 cut(s) 614
NspI RCATGY 3 cut(s) 416, 476, 1104
NspV TTCGAA 1 cut(s) 71
PciI ACATGT 1 cut(s) 472
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PctI GAATGC 3 cut(s) 371, 868, 945
PdmI GAANNNNTTC 2 cut(s) 681, 838
PfeI GAWTC 2 cut(s) 5, 677
PkrI GCNGC 5 cut(s) 58, 61, 84, 142, 228
PleI GAGTC 2 cut(s) 731, 867
PluTI GGCGCC 1 cut(s) 110
PpsI GAGTC 2 cut(s) 731, 867
PscI ACATGT 1 cut(s) 472
PsiI TTATAA 1 cut(s) 569
Psp6I CCWGG 2 cut(s) 462, 641
PspFI CCCAGC 1 cut(s) 900
PspGI CCWGG 2 cut(s) 462, 641
PspN4I GGNNCC 2 cut(s) 108, 899
PspPI GGNCC 2 cut(s) 664, 897
PsuI RGATCY 1 cut(s) 805
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
RseI CAYNNNNRTG 1 cut(s) 417
SaqAI TTAA 4 cut(s) 435, 477, 525, 714
SatI GCNGC 5 cut(s) 57, 60, 83, 141, 227
Sau3AI GATC 2 cut(s) 256, 805
Sau96I GGNCC 2 cut(s) 664, 897
SchI GAGTC 2 cut(s) 731, 868
ScrFI CCNGG 3 cut(s) 464, 585, 643
SduI GDGCHC 1 cut(s) 791
SfaNI GCATC 4 cut(s) 204, 226, 438, 1044
SfoI GGCGCC 1 cut(s) 108
SfuI TTCGAA 1 cut(s) 71
SinI GGWCC 2 cut(s) 664, 897
SmiMI CAYNNNNRTG 1 cut(s) 417
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
Sse9I AATT 7 cut(s) 67, 88, 387, 554, 724, 838, 844
SsiI CCGC 7 cut(s) 56, 59, 140, 208, 226, 233, 239
SspDI GGCGCC 1 cut(s) 106
StyD4I CCNGG 3 cut(s) 462, 583, 641
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 3 cut(s) 150, 664, 958
TaiI ACGT 1 cut(s) 654
TaqI TCGA 4 cut(s) 71, 113, 219, 735
TasI AATT 7 cut(s) 67, 88, 387, 554, 724, 838, 844
TatI WGTACW 1 cut(s) 150
TauI GCSGC 4 cut(s) 59, 62, 143, 229
TfiI GAWTC 2 cut(s) 5, 677
Tru1I TTAA 4 cut(s) 435, 477, 525, 714
Tru9I TTAA 4 cut(s) 435, 477, 525, 714
TscAI CASTG 1 cut(s) 926
TseFI GTSAC 2 cut(s) 118, 1019
TseI GCWGC 1 cut(s) 82
Tsp45I GTSAC 2 cut(s) 118, 1019
TspDTI ATGAA 4 cut(s) 194, 278, 440, 767
TspGWI ACGGA 2 cut(s) 311, 910
TspRI CASTG 1 cut(s) 926
Vha464I CTTAAG 1 cut(s) 434
VpaK11BI GGWCC 2 cut(s) 664, 897
XapI RAATTY 6 cut(s) 67, 88, 387, 554, 838, 844
XceI RCATGY 3 cut(s) 416, 476, 1104
XmnI GAANNNNTTC 2 cut(s) 681, 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.