MD11G1001400.v1.1

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0028894
Physical Location & Seq
Reverse (-)
171020 .. 171348
329 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1001400.v1.1.491

Sequence Viewer

Length: 282 bp
ATGCCTATACTGTCTGTTGTTGTCCCCGAGATCAAGACAGATTCATTTGACTGGTCATGCAGTGATGAAGCTCCAGCAAGGCATAGTCTGATTTTCCGTGGGTTGGTTCCAGTTCTAAGCGCGGGATCTTCTAGGGCCTCTCACGCAGAGACGACCGAAGAAGCACTGGACTTTGCCCTTCAACATGCCAAGACAAAGGGGCTCTGCAAGAACGGAGATGCTGTTGTGGCTCTGCACCGTGATGGAACTGCATCAGTGATCAAGATCTTGACCGTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

94

Amino Acids

9.91

Weight (kDa)

6.39

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_C PF02887 13 - 88 8.1e-08 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028894)

Species Orthologous Gene IDs
malus_domestica MD11G1001400.v1.1
pyrus_communis pycom09g08600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 122
AciI CCGC 1 cut(s) 122
AclWI GGATC 1 cut(s) 133
AfiI CCNNNNNNNGG 1 cut(s) 103
AgsI TTSAA 1 cut(s) 182
AluBI AGCT 1 cut(s) 71
AluI AGCT 1 cut(s) 71
Alw26I GTCTC 1 cut(s) 143
AlwI GGATC 1 cut(s) 133
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 1 cut(s) 135
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 135
AvaI CYCGRG 1 cut(s) 26
BanII GRGCYC 1 cut(s) 204
BccI CCATC 1 cut(s) 236
BclI TGATCA 1 cut(s) 258
BcoDI GTCTC 1 cut(s) 143
BfaI CTAG 1 cut(s) 132
BglII AGATCT 1 cut(s) 264
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 135
BmiI GGNNCC 1 cut(s) 108
BmsI GCATC 2 cut(s) 208, 260
BpmI CTGGAG 1 cut(s) 57
BsaBI GATNNNNATC 1 cut(s) 263
BsaJI CCNNGG 1 cut(s) 97
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse1I ACTGG 3 cut(s) 56, 110, 171
Bse8I GATNNNNATC 1 cut(s) 263
BseDI CCNNGG 1 cut(s) 97
BseJI GATNNNNATC 1 cut(s) 263
BseLI CCNNNNNNNGG 1 cut(s) 103
BseNI ACTGG 3 cut(s) 56, 110, 171
BsgI GTGCAG 1 cut(s) 218
Bsh1236I CGCG 1 cut(s) 122
Bsh1285I CGRYCG 1 cut(s) 156
BshFI GGCC 1 cut(s) 137
BsiEI CGRYCG 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 26
BslFI GGGAC 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 103
BsmAI GTCTC 1 cut(s) 143
BsmBI CGTCTC 1 cut(s) 143
BsmFI GGGAC 1 cut(s) 8
BsnI GGCC 1 cut(s) 137
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 204
Bsp143I GATC 4 cut(s) 30, 125, 258, 264
BspACI CCGC 1 cut(s) 122
BspANI GGCC 1 cut(s) 137
BspFNI CGCG 1 cut(s) 122
BspLI GGNNCC 1 cut(s) 108
BspPI GGATC 1 cut(s) 133
BsrI ACTGG 3 cut(s) 56, 110, 171
BssECI CCNNGG 1 cut(s) 97
BssMI GATC 4 cut(s) 30, 125, 258, 264
Bst4CI ACNGT 3 cut(s) 12, 239, 274
BstDEI CTNAG 1 cut(s) 116
BstDSI CCRYGG 1 cut(s) 97
BstFNI CGCG 1 cut(s) 122
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 4 cut(s) 33, 128, 261, 267
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 4 cut(s) 30, 125, 258, 264
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 2 cut(s) 143, 227
BstNSI RCATGY 1 cut(s) 188
BstUI CGCG 1 cut(s) 122
BstX2I RGATCY 2 cut(s) 125, 264
BstYI RGATCY 2 cut(s) 125, 264
BsuRI GGCC 1 cut(s) 137
BtgI CCRYGG 1 cut(s) 97
BtsI GCAGTG 1 cut(s) 67
BtsIMutI CAGTG 3 cut(s) 67, 164, 261
CfoI GCGC 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 135
CviAII CATG 2 cut(s) 57, 185
CviJI RGCY 4 cut(s) 71, 137, 202, 230
CviKI_1 RGCY 4 cut(s) 71, 137, 202, 230
DdeI CTNAG 1 cut(s) 116
DpnI GATC 4 cut(s) 32, 127, 260, 266
DpnII GATC 4 cut(s) 30, 125, 258, 264
Eco24I GRGCYC 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 26
EcoO109I RGGNCCY 1 cut(s) 135
EcoT38I GRGCYC 1 cut(s) 204
Esp3I CGTCTC 1 cut(s) 143
FaeI CATG 2 cut(s) 60, 188
FaiI YATR 4 cut(s) 8, 58, 84, 186
FaqI GGGAC 1 cut(s) 8
FatI CATG 2 cut(s) 56, 184
FauI CCCGC 1 cut(s) 115
FbaI TGATCA 1 cut(s) 258
FriOI GRGCYC 1 cut(s) 204
FspBI CTAG 1 cut(s) 132
GlaI GCGC 1 cut(s) 121
GsuI CTGGAG 1 cut(s) 57
HaeIII GGCC 1 cut(s) 137
HhaI GCGC 1 cut(s) 122
Hin1II CATG 2 cut(s) 60, 188
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HinfI GANTC 1 cut(s) 41
Hpy188I TCNGA 1 cut(s) 90
Hpy188III TCNNGA 3 cut(s) 34, 262, 268
HpyAV CCTTC 1 cut(s) 188
HpyCH4III ACNGT 3 cut(s) 12, 239, 274
HpyCH4V TGCA 4 cut(s) 60, 207, 235, 251
HpyF10VI GCNNNNNNNGC 2 cut(s) 143, 227
HpyF3I CTNAG 1 cut(s) 116
Hsp92II CATG 2 cut(s) 60, 188
HspAI GCGC 1 cut(s) 120
Ksp22I TGATCA 1 cut(s) 258
Kzo9I GATC 4 cut(s) 30, 125, 258, 264
LmnI GCTCC 1 cut(s) 76
LpnPI CCDG 4 cut(s) 37, 87, 123, 152
LweI GCATC 2 cut(s) 208, 260
MaeI CTAG 1 cut(s) 132
MalI GATC 4 cut(s) 32, 127, 260, 266
MboI GATC 4 cut(s) 30, 125, 258, 264
MboII GAAGA 2 cut(s) 120, 170
MflI RGATCY 2 cut(s) 125, 264
MhlI GDGCHC 1 cut(s) 204
MnlI CCTC 1 cut(s) 148
MslI CAYNNNNRTG 1 cut(s) 240
MvnI CGCG 1 cut(s) 122
MwoI GCNNNNNNNGC 2 cut(s) 143, 227
NdeII GATC 4 cut(s) 30, 125, 258, 264
NlaIII CATG 2 cut(s) 60, 188
NlaIV GGNNCC 1 cut(s) 108
NspI RCATGY 1 cut(s) 188
PfeI GAWTC 1 cut(s) 41
PspN4I GGNNCC 1 cut(s) 108
PspPI GGNCC 1 cut(s) 135
PsuI RGATCY 2 cut(s) 125, 264
RseI CAYNNNNRTG 1 cut(s) 240
Sau3AI GATC 4 cut(s) 30, 125, 258, 264
Sau96I GGNCC 1 cut(s) 135
SduI GDGCHC 1 cut(s) 204
SetI ASST 1 cut(s) 73
SfaNI GCATC 2 cut(s) 208, 260
SmiMI CAYNNNNRTG 1 cut(s) 240
SsiI CCGC 1 cut(s) 122
SspMI CTAG 1 cut(s) 132
TaaI ACNGT 3 cut(s) 12, 239, 274
TaqII GACCGA 1 cut(s) 170
TfiI GAWTC 1 cut(s) 41
TscAI CASTG 3 cut(s) 67, 171, 261
TspDTI ATGAA 2 cut(s) 33, 81
TspGWI ACGGA 2 cut(s) 86, 228
TspRI CASTG 3 cut(s) 67, 171, 261
XceI RCATGY 1 cut(s) 188
XspI CTAG 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.