pycom09g08600

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0028894
Physical Location & Seq
Forward (+)
6644841 .. 6645113
273 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g08600.1

Sequence Viewer

Length: 273 bp
ATGCCAATTTTGTCCGTGGTGGTTCCTGAGATAACAACAGCTTATTTTGAGACCTCATGCAGCAATGCAGCTCCGGCAAGGCATGACCTTGTTTACTGTGGCGTTCTGCCGCTTCTCCCCTCAGCTCCCGTTAGAGCTTCTCATGCCGAGTCCACAGAAGAGAGCATACAGTTTGCAGTTGAATTTGCAAAGGCAAAGGGACTCTGCAAGCTTGGAGACGAAGTCGTGGCTTTGCACGGCGCTAACGCTGATATGATCAAGATTTGTTTGTGA

Protein Analysis

91

Amino Acids

9.53

Weight (kDa)

5.15

Isoelectric Point (pI)

47.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_C PF02887 2 - 88 2.3e-07 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0028894)

Species Orthologous Gene IDs
malus_domestica MD11G1001400.v1.1
pyrus_communis pycom09g08600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 110
AcsI RAATTY 1 cut(s) 182
AgsI TTSAA 1 cut(s) 182
AluBI AGCT 5 cut(s) 41, 71, 125, 137, 211
AluI AGCT 5 cut(s) 41, 71, 125, 137, 211
Alw26I GTCTC 2 cut(s) 44, 210
ApeKI GCWGC 2 cut(s) 60, 68
ApoI RAATTY 1 cut(s) 182
AspLEI GCGC 1 cut(s) 242
BarI GAAGNNNNNNTAC 2 cut(s) 150, 182
BbvCI CCTCAGC 1 cut(s) 121
BbvI GCAGC 2 cut(s) 72, 80
BceAI ACGGC 1 cut(s) 253
BclI TGATCA 1 cut(s) 255
BcoDI GTCTC 2 cut(s) 44, 210
BfoI RGCGCY 1 cut(s) 243
BisI GCNGC 3 cut(s) 61, 69, 110
BlsI GCNGC 3 cut(s) 62, 70, 111
BmiI GGNNCC 1 cut(s) 24
Bpu10I CCTNAGC 1 cut(s) 121
BsaI GGTCTC 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 15
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bse3DI GCAATG 1 cut(s) 70
BseDI CCNNGG 1 cut(s) 15
BseMI GCAATG 1 cut(s) 70
BseMII CTCAG 2 cut(s) 18, 135
BseXI GCAGC 2 cut(s) 72, 80
BsiSI CCGG 1 cut(s) 74
BslFI GGGAC 1 cut(s) 213
BsmAI GTCTC 2 cut(s) 44, 210
BsmBI CGTCTC 1 cut(s) 210
BsmFI GGGAC 1 cut(s) 213
Bso31I GGTCTC 1 cut(s) 44
Bsp143I GATC 1 cut(s) 255
BspACI CCGC 1 cut(s) 110
BspCNI CTCAG 2 cut(s) 19, 134
BspLI GGNNCC 1 cut(s) 24
BspTNI GGTCTC 1 cut(s) 44
BsrDI GCAATG 1 cut(s) 70
BssECI CCNNGG 1 cut(s) 15
BssMI GATC 1 cut(s) 255
Bst4CI ACNGT 2 cut(s) 98, 171
Bst6I CTCTTC 1 cut(s) 153
BstC8I GCNNGC 1 cut(s) 209
BstDEI CTNAG 2 cut(s) 27, 121
BstDSI CCRYGG 1 cut(s) 15
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 1 cut(s) 242
BstKTI GATC 1 cut(s) 258
BstMAI GTCTC 2 cut(s) 44, 210
BstMBI GATC 1 cut(s) 255
BstMWI GCNNNNNNNGC 2 cut(s) 74, 143
BstV1I GCAGC 2 cut(s) 72, 80
BtgI CCRYGG 1 cut(s) 15
Cac8I GCNNGC 1 cut(s) 209
CfoI GCGC 1 cut(s) 242
CviAII CATG 3 cut(s) 57, 83, 143
CviJI RGCY 6 cut(s) 41, 71, 125, 137, 211, 230
CviKI_1 RGCY 6 cut(s) 41, 71, 125, 137, 211, 230
DdeI CTNAG 2 cut(s) 27, 121
DpnI GATC 1 cut(s) 257
DpnII GATC 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
Eco31I GGTCTC 1 cut(s) 44
Esp3I CGTCTC 1 cut(s) 210
FaeI CATG 3 cut(s) 60, 86, 146
FaiI YATR 5 cut(s) 58, 84, 144, 167, 254
FaqI GGGAC 1 cut(s) 213
FatI CATG 3 cut(s) 56, 82, 142
FbaI TGATCA 1 cut(s) 255
Fnu4HI GCNGC 3 cut(s) 61, 69, 110
Fsp4HI GCNGC 3 cut(s) 61, 69, 110
GlaI GCGC 1 cut(s) 241
GluI GCNGC 3 cut(s) 61, 69, 110
HaeII RGCGCY 1 cut(s) 243
HapII CCGG 1 cut(s) 74
HhaI GCGC 1 cut(s) 242
Hin1II CATG 3 cut(s) 60, 86, 146
Hin6I GCGC 1 cut(s) 240
HinP1I GCGC 1 cut(s) 240
HindIII AAGCTT 1 cut(s) 209
HinfI GANTC 2 cut(s) 149, 201
HpaII CCGG 1 cut(s) 74
Hpy166II GTNNAC 2 cut(s) 94, 153
Hpy188III TCNNGA 2 cut(s) 26, 259
Hpy8I GTNNAC 2 cut(s) 94, 153
HpyCH4III ACNGT 2 cut(s) 98, 171
HpyCH4V TGCA 6 cut(s) 60, 68, 176, 188, 207, 235
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 143
HpyF3I CTNAG 2 cut(s) 27, 121
Hsp92II CATG 3 cut(s) 60, 86, 146
HspAI GCGC 1 cut(s) 240
Ksp22I TGATCA 1 cut(s) 255
Kzo9I GATC 1 cut(s) 255
LmnI GCTCC 2 cut(s) 76, 130
LpnPI CCDG 2 cut(s) 39, 87
Lsp1109I GCAGC 2 cut(s) 72, 80
MalI GATC 1 cut(s) 257
MboI GATC 1 cut(s) 255
MboII GAAGA 1 cut(s) 170
MluCI AATT 2 cut(s) 6, 182
MlyI GAGTC 2 cut(s) 158, 195
MnlI CCTC 2 cut(s) 64, 130
MspI CCGG 1 cut(s) 74
MwoI GCNNNNNNNGC 2 cut(s) 74, 143
NdeII GATC 1 cut(s) 255
NlaIII CATG 3 cut(s) 60, 86, 146
NlaIV GGNNCC 1 cut(s) 24
NmeAIII GCCGAG 1 cut(s) 172
PflFI GACNNNGTC 1 cut(s) 221
PkrI GCNGC 3 cut(s) 62, 70, 111
PleI GAGTC 2 cut(s) 157, 195
PpsI GAGTC 2 cut(s) 157, 195
PspN4I GGNNCC 1 cut(s) 24
PsyI GACNNNGTC 1 cut(s) 221
SatI GCNGC 3 cut(s) 61, 69, 110
Sau3AI GATC 1 cut(s) 255
SchI GAGTC 2 cut(s) 158, 195
SetI ASST 7 cut(s) 43, 56, 73, 90, 127, 139, 213
Sse9I AATT 2 cut(s) 6, 182
SsiI CCGC 1 cut(s) 110
TaaI ACNGT 2 cut(s) 98, 171
TasI AATT 2 cut(s) 6, 182
TauI GCSGC 1 cut(s) 112
TseI GCWGC 2 cut(s) 60, 68
TspGWI ACGGA 1 cut(s) 4
Tth111I GACNNNGTC 1 cut(s) 221
XapI RAATTY 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.