MD11G1001600.v1.1

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0028922
Physical Location & Seq
Reverse (-)
172263 .. 173842
1580 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1001600.v1.1.491

Sequence Viewer

Length: 561 bp
ATGGATCAGAGGCCCAAGACCAAGATCGTGTGCACCCTTGGACCCGCATCGCGGTCTGTTCCGATGGTGGAGAAGCTGCTGAGGGCCGGCATGAACGTCGCCCGCTTCAACTTCTCCCATGGGTCCCACGAGTACCATCAGGAGACCCTCAACAATCTCAGGGCCGCCATGGAATCCACCGGCATCCTTTGCGCCGTCATGCTCGACACCAAGGGTCCAGAGATTCGAACAGGGTTTCTCAAAGATGAGAAACCTGTCCAGCTCAAACAGGGCCAAGAGATCACCATCTCCACTGACTATACCCTCAAGGGTGATGCAAATATGATTTGTATGAGTTACAAAAAGCTGGCAGAGGATGTCAAGCCAGGGAGCATGATTCTATGTGCTGATGGCACCATCTCCTTTATGGTTTTGTCTTGTGACAAGCAAAAGGGTTTGGTTCGATGCCGCTGTGAGAACTCTGCAGTTCTTGGTGAGAGGAAGAACGTTAATCTTCCTGGGGTCGTTGTGGATCTCCCAACCTTAACAGAGAAAGACAAAGAAGATATTCTCAAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

187

Amino Acids

20.6

Weight (kDa)

8.44

Isoelectric Point (pI)

32.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 6 - 183 8.3e-69 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028922)

Species Orthologous Gene IDs
malus_domestica MD11G1001600.v1.1
pyrus_communis pycom03g00170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 392
AccII CGCG 1 cut(s) 52
AciI CCGC 5 cut(s) 45, 52, 103, 165, 448
AclI AACGTT 1 cut(s) 486
AclWI GGATC 2 cut(s) 12, 519
AfaI GTAC 1 cut(s) 134
AfiI CCNNNNNNNGG 1 cut(s) 51
AgsI TTSAA 1 cut(s) 109
AjnI CCWGG 2 cut(s) 364, 496
AluBI AGCT 3 cut(s) 76, 262, 346
AluI AGCT 3 cut(s) 76, 262, 346
Alw21I GWGCWC 1 cut(s) 35
Alw26I GTCTC 1 cut(s) 137
Alw44I GTGCAC 1 cut(s) 31
AlwI GGATC 2 cut(s) 12, 519
AoxI GGCC 4 cut(s) 11, 84, 162, 271
ApaLI GTGCAC 1 cut(s) 31
ApeKI GCWGC 1 cut(s) 76
Asp700I GAANNNNTTC 1 cut(s) 546
AspLEI GCGC 1 cut(s) 194
AspS9I GGNCC 7 cut(s) 12, 41, 84, 123, 162, 215, 271
AsuHPI GGTGA 3 cut(s) 274, 323, 485
AsuII TTCGAA 1 cut(s) 226
AvaII GGWCC 3 cut(s) 41, 123, 215
BaeGI GKGCMC 1 cut(s) 35
BanI GGYRCC 1 cut(s) 392
BauI CACGAG 1 cut(s) 128
Bbv12I GWGCWC 1 cut(s) 35
BbvCI CCTCAGC 1 cut(s) 80
BbvI GCAGC 1 cut(s) 63
BccI CCATC 5 cut(s) 58, 144, 293, 383, 404
BceAI ACGGC 1 cut(s) 179
BcgI CGANNNNNNTGC 2 cut(s) 79, 113
BciT130I CCWGG 2 cut(s) 366, 498
BcoDI GTCTC 1 cut(s) 137
BfmI CTRYAG 1 cut(s) 462
BisI GCNGC 3 cut(s) 77, 165, 448
BlsI GCNGC 3 cut(s) 78, 166, 449
Bme1390I CCNGG 2 cut(s) 366, 498
Bme18I GGWCC 3 cut(s) 41, 123, 215
BmgT120I GGNCC 7 cut(s) 12, 41, 84, 123, 162, 215, 271
BmiI GGNNCC 5 cut(s) 43, 124, 125, 216, 394
BmrFI CCNGG 2 cut(s) 366, 498
BmsI GCATC 4 cut(s) 56, 192, 304, 434
Bpu10I CCTNAGC 1 cut(s) 80
Bpu14I TTCGAA 1 cut(s) 226
BpuEI CTTGAG 2 cut(s) 290, 536
BsaBI GATNNNNATC 1 cut(s) 284
BsaI GGTCTC 1 cut(s) 137
BsaJI CCNNGG 6 cut(s) 37, 118, 168, 210, 365, 497
Bsc4I CCNNNNNNNGG 1 cut(s) 51
Bse118I RCCGGY 2 cut(s) 86, 179
Bse8I GATNNNNATC 1 cut(s) 284
BseBI CCWGG 2 cut(s) 366, 498
BseDI CCNNGG 6 cut(s) 37, 118, 168, 210, 365, 497
BseGI GGATG 2 cut(s) 183, 361
BseJI GATNNNNATC 1 cut(s) 284
BseLI CCNNNNNNNGG 1 cut(s) 51
BseMII CTCAG 2 cut(s) 71, 172
BseSI GKGCMC 1 cut(s) 35
BseXI GCAGC 1 cut(s) 63
Bsh1236I CGCG 1 cut(s) 52
BshFI GGCC 4 cut(s) 13, 86, 164, 273
BshNI GGYRCC 1 cut(s) 392
BsiHKAI GWGCWC 1 cut(s) 35
BsiSI CCGG 2 cut(s) 87, 180
BslFI GGGAC 1 cut(s) 109
BslI CCNNNNNNNGG 1 cut(s) 51
BsmAI GTCTC 1 cut(s) 137
BsmFI GGGAC 1 cut(s) 109
BsnI GGCC 4 cut(s) 13, 86, 164, 273
Bso31I GGTCTC 1 cut(s) 137
Bsp119I TTCGAA 1 cut(s) 226
Bsp1286I GDGCHC 1 cut(s) 35
Bsp143I GATC 4 cut(s) 4, 24, 279, 511
Bsp19I CCATGG 2 cut(s) 118, 168
BspACI CCGC 5 cut(s) 45, 52, 103, 165, 448
BspANI GGCC 4 cut(s) 13, 86, 164, 273
BspCNI CTCAG 2 cut(s) 72, 171
BspFNI CGCG 1 cut(s) 52
BspLI GGNNCC 5 cut(s) 43, 124, 125, 216, 394
BspMAI CTGCAG 1 cut(s) 466
BspPI GGATC 2 cut(s) 12, 519
BspT104I TTCGAA 1 cut(s) 226
BspT107I GGYRCC 1 cut(s) 392
BspTNI GGTCTC 1 cut(s) 137
BsrFI RCCGGY 2 cut(s) 86, 179
BssAI RCCGGY 2 cut(s) 86, 179
BssECI CCNNGG 6 cut(s) 37, 118, 168, 210, 365, 497
BssMI GATC 4 cut(s) 4, 24, 279, 511
BssSI CACGAG 1 cut(s) 128
BssT1I CCWWGG 4 cut(s) 37, 118, 168, 210
Bst2BI CACGAG 1 cut(s) 128
Bst2UI CCWGG 2 cut(s) 366, 498
BstAPI GCANNNNNTGC 1 cut(s) 189
BstBI TTCGAA 1 cut(s) 226
BstC8I GCNNGC 3 cut(s) 88, 103, 348
BstDEI CTNAG 2 cut(s) 80, 158
BstDSI CCRYGG 2 cut(s) 118, 168
BstF5I GGATG 2 cut(s) 183, 361
BstFNI CGCG 1 cut(s) 52
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 4 cut(s) 7, 27, 282, 514
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 4 cut(s) 4, 24, 279, 511
BstMWI GCNNNNNNNGC 1 cut(s) 189
BstNI CCWGG 2 cut(s) 366, 498
BstSCI CCNGG 2 cut(s) 364, 496
BstSFI CTRYAG 1 cut(s) 462
BstSLI GKGCMC 1 cut(s) 35
BstUI CGCG 1 cut(s) 52
BstV1I GCAGC 1 cut(s) 63
BstX2I RGATCY 1 cut(s) 511
BstYI RGATCY 1 cut(s) 511
BsuRI GGCC 4 cut(s) 13, 86, 164, 273
BtgI CCRYGG 2 cut(s) 118, 168
BtgZI GCGATG 1 cut(s) 33
BtsCI GGATG 2 cut(s) 183, 361
BtsIMutI CAGTG 1 cut(s) 291
Cac8I GCNNGC 3 cut(s) 88, 103, 348
CfoI GCGC 1 cut(s) 194
Cfr10I RCCGGY 2 cut(s) 86, 179
Cfr13I GGNCC 7 cut(s) 12, 41, 84, 123, 162, 215, 271
Csp6I GTAC 1 cut(s) 133
CviAII CATG 5 cut(s) 91, 119, 169, 199, 373
CviJI RGCY 8 cut(s) 13, 76, 86, 164, 262, 273, 346, 364
CviKI_1 RGCY 8 cut(s) 13, 76, 86, 164, 262, 273, 346, 364
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 2 cut(s) 80, 158
DpnI GATC 4 cut(s) 6, 26, 281, 513
DpnII GATC 4 cut(s) 4, 24, 279, 511
Eco130I CCWWGG 4 cut(s) 37, 118, 168, 210
Eco31I GGTCTC 1 cut(s) 137
Eco47I GGWCC 3 cut(s) 41, 123, 215
EcoO109I RGGNCCY 1 cut(s) 123
EcoRII CCWGG 2 cut(s) 364, 496
EcoT14I CCWWGG 4 cut(s) 37, 118, 168, 210
ErhI CCWWGG 4 cut(s) 37, 118, 168, 210
FaeI CATG 5 cut(s) 94, 122, 172, 202, 376
FaqI GGGAC 1 cut(s) 109
FatI CATG 5 cut(s) 90, 118, 168, 198, 372
FauI CCCGC 2 cut(s) 52, 110
Fnu4HI GCNGC 3 cut(s) 77, 165, 448
FokI GGATG 2 cut(s) 170, 368
Fsp4HI GCNGC 3 cut(s) 77, 165, 448
GlaI GCGC 1 cut(s) 193
GluI GCNGC 3 cut(s) 77, 165, 448
HaeIII GGCC 4 cut(s) 13, 86, 164, 273
HapII CCGG 2 cut(s) 87, 180
HhaI GCGC 1 cut(s) 194
Hin1II CATG 5 cut(s) 94, 122, 172, 202, 376
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HinfI GANTC 3 cut(s) 173, 223, 376
HpaII CCGG 2 cut(s) 87, 180
HphI GGTGA 3 cut(s) 274, 323, 485
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 3 cut(s) 9, 63, 560
Hpy188III TCNNGA 2 cut(s) 140, 218
Hpy8I GTNNAC 1 cut(s) 33
Hpy99I CGWCG 1 cut(s) 101
HpyCH4IV ACGT 2 cut(s) 96, 486
HpyCH4V TGCA 3 cut(s) 33, 317, 464
HpyF10VI GCNNNNNNNGC 1 cut(s) 189
HpyF3I CTNAG 2 cut(s) 80, 158
HpySE526I ACGT 2 cut(s) 96, 486
Hsp92II CATG 5 cut(s) 94, 122, 172, 202, 376
HspAI GCGC 1 cut(s) 192
KflI GGGWCCC 1 cut(s) 123
KroI GCCGGC 1 cut(s) 86
KroNI GCCGGC 1 cut(s) 88
Kzo9I GATC 4 cut(s) 4, 24, 279, 511
LmnI GCTCC 1 cut(s) 369
Lsp1109I GCAGC 1 cut(s) 63
LweI GCATC 4 cut(s) 56, 192, 304, 434
MaeII ACGT 2 cut(s) 96, 486
MaeIII GTNAC 2 cut(s) 335, 419
MalI GATC 4 cut(s) 6, 26, 281, 513
MboI GATC 4 cut(s) 4, 24, 279, 511
MboII GAAGA 3 cut(s) 485, 493, 554
MflI RGATCY 1 cut(s) 511
MhlI GDGCHC 1 cut(s) 35
MnlI CCTC 6 cut(s) 3, 75, 158, 314, 346, 471
MroNI GCCGGC 1 cut(s) 86
MroXI GAANNNNTTC 1 cut(s) 546
MseI TTAA 2 cut(s) 489, 524
MspA1I CMGCKG 1 cut(s) 450
MspI CCGG 2 cut(s) 87, 180
MspR9I CCNGG 2 cut(s) 366, 498
MvaI CCWGG 2 cut(s) 366, 498
MvnI CGCG 1 cut(s) 52
MwoI GCNNNNNNNGC 1 cut(s) 189
NaeI GCCGGC 1 cut(s) 88
NcoI CCATGG 2 cut(s) 118, 168
NdeII GATC 4 cut(s) 4, 24, 279, 511
NgoMIV GCCGGC 1 cut(s) 86
NlaIII CATG 5 cut(s) 94, 122, 172, 202, 376
NlaIV GGNNCC 5 cut(s) 43, 124, 125, 216, 394
NmuCI GTSAC 1 cut(s) 419
NspV TTCGAA 1 cut(s) 226
PdiI GCCGGC 1 cut(s) 88
PdmI GAANNNNTTC 1 cut(s) 546
PfeI GAWTC 3 cut(s) 173, 223, 376
PkrI GCNGC 3 cut(s) 78, 166, 449
PpuMI RGGWCCY 1 cut(s) 123
Psp1406I AACGTT 1 cut(s) 486
Psp5II RGGWCCY 1 cut(s) 123
Psp6I CCWGG 2 cut(s) 364, 496
PspGI CCWGG 2 cut(s) 364, 496
PspN4I GGNNCC 5 cut(s) 43, 124, 125, 216, 394
PspPI GGNCC 7 cut(s) 12, 41, 84, 123, 162, 215, 271
PspPPI RGGWCCY 1 cut(s) 123
PstI CTGCAG 1 cut(s) 466
PsuI RGATCY 1 cut(s) 511
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
SaqAI TTAA 2 cut(s) 489, 524
SatI GCNGC 3 cut(s) 77, 165, 448
Sau3AI GATC 4 cut(s) 4, 24, 279, 511
Sau96I GGNCC 7 cut(s) 12, 41, 84, 123, 162, 215, 271
ScrFI CCNGG 2 cut(s) 366, 498
SduI GDGCHC 1 cut(s) 35
SetI ASST 7 cut(s) 78, 99, 256, 264, 348, 489, 524
SfaNI GCATC 4 cut(s) 56, 192, 304, 434
SfcI CTRYAG 1 cut(s) 462
SfuI TTCGAA 1 cut(s) 226
SinI GGWCC 3 cut(s) 41, 123, 215
SmlI CTYRAG 2 cut(s) 305, 551
SmoI CTYRAG 2 cut(s) 305, 551
SsiI CCGC 5 cut(s) 45, 52, 103, 165, 448
StyD4I CCNGG 2 cut(s) 364, 496
StyI CCWWGG 4 cut(s) 37, 118, 168, 210
TaiI ACGT 2 cut(s) 99, 489
TaqI TCGA 3 cut(s) 204, 226, 442
TauI GCSGC 2 cut(s) 167, 450
TfiI GAWTC 3 cut(s) 173, 223, 376
Tru1I TTAA 2 cut(s) 489, 524
Tru9I TTAA 2 cut(s) 489, 524
TscAI CASTG 1 cut(s) 298
TseFI GTSAC 1 cut(s) 419
TseI GCWGC 1 cut(s) 76
Tsp45I GTSAC 1 cut(s) 419
TspDTI ATGAA 1 cut(s) 107
TspRI CASTG 1 cut(s) 298
VneI GTGCAC 1 cut(s) 31
VpaK11BI GGWCC 3 cut(s) 41, 123, 215
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XmnI GAANNNNTTC 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.