pycom03g00170

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0028922
Physical Location & Seq
Reverse (-)
166583 .. 168673
2091 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g00170.1

Sequence Viewer

Length: 969 bp
ATGAAATTTTTAGATTTATTTGCGAGAGTTGAGTCATTCACGTGCGAACACAAGACAAGTACATCGCCGTTGGCATATTTTCCCACAACACTCTTTCAACTCCCCCACCCAATAACAACAACGACAACAACAGAAAAAGCTGAAAGCCGAAAGAAATCGGAGGGAAAAATGGATCAGAGGCCCAAGACCAAGATCGTCTGCACCCTGGGACCCGCGTCGCGGTCCGTTCCCATGGTGGAGAAGCTGCTGAGGGCCGGCATGAACGTCGCCCGCTTCAACTTCTCCCATGGGTCCCACGAGTACCATCAGGAGACCCTCGACAATCTCAGGGCAGCCATGGAATCCACCGGCATCCTTTGCGCCGTCATGCTCGACACCAAGGGTCCAGAGATTCGAACTGGATTTCTCAAGGATGCGAAACCCGTCCAGCTCAAACTGGGCCAAGAGATCACCATTTCCACTGATTATAGCCTCAAGGGTGATGCAAATATGATTTGTATGAGTTACAAAAAGCTGGCTGAGGATGTCAAGCCGGGGAGCATGATTCTATGTGCTGATGGCACCATCTCCTTTATGGTTCTGTCTTGCGATAAGCAAAAGGGTTTGGTTCAATGCCGCTGTGAGAACTCTGCAGTTCTTGGTGAGAGGAAGAACGTTAATCTTCCCGGGGTCATTGTGGATCTCCCAACCTTAACAGAGAAAGACAAAGAAGATATTCTCAAGTGGGGAGTTCCAAATAAGATTGACATGATTGCCCTGTCTTTTGTTCGGAAAGGATCGATCTTGTGGAGGTCAGGAAGCTCCTTGGAAAGCATTCTAAGAATATCCTTCTCATGTCAAAGGTTGAGAATCAGGAAGGGGTGGCGAACTTTGATGACATCCTTGCACAATCAGATGCATTCATGGTGGCACGAGGTGATCTTGGTATGGAAATTCCAATTGAAAAGATCTTCCTTGCTCAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

323

Amino Acids

36.42

Weight (kDa)

9.51

Isoelectric Point (pI)

42.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 62 - 260 5.8e-76 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028922)

Species Orthologous Gene IDs
malus_domestica MD11G1001600.v1.1
pyrus_communis pycom03g00170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 560
AccII CGCG 2 cut(s) 215, 220
AciI CCGC 4 cut(s) 213, 220, 271, 616
AclI AACGTT 1 cut(s) 654
AclWI GGATC 3 cut(s) 180, 687, 784
AcsI RAATTY 2 cut(s) 5, 932
AcvI CACGTG 1 cut(s) 42
AdeI CACNNNGTG 1 cut(s) 916
AfaI GTAC 2 cut(s) 61, 302
AfiI CCNNNNNNNGG 1 cut(s) 219
AgsI TTSAA 4 cut(s) 98, 277, 611, 943
AjnI CCWGG 1 cut(s) 204
AluBI AGCT 5 cut(s) 140, 244, 430, 514, 801
AluI AGCT 5 cut(s) 140, 244, 430, 514, 801
Alw26I GTCTC 1 cut(s) 305
AlwI GGATC 3 cut(s) 180, 687, 784
Ama87I CYCGRG 1 cut(s) 665
AoxI GGCC 3 cut(s) 179, 252, 439
ApeKI GCWGC 2 cut(s) 244, 332
ApoI RAATTY 2 cut(s) 5, 932
Asp700I GAANNNNTTC 2 cut(s) 714, 813
AspLEI GCGC 1 cut(s) 362
AspS9I GGNCC 7 cut(s) 180, 209, 222, 252, 291, 383, 439
AsuC2I CCSGG 3 cut(s) 534, 666, 667
AsuHPI GGTGA 4 cut(s) 442, 491, 653, 928
AsuII TTCGAA 1 cut(s) 394
AvaI CYCGRG 1 cut(s) 665
AvaII GGWCC 4 cut(s) 209, 222, 291, 383
BanI GGYRCC 1 cut(s) 560
BauI CACGAG 2 cut(s) 296, 911
BbrPI CACGTG 1 cut(s) 42
BbvCI CCTCAGC 2 cut(s) 248, 519
BbvI GCAGC 2 cut(s) 231, 344
BccI CCATC 3 cut(s) 312, 551, 572
BceAI ACGGC 2 cut(s) 52, 347
BcgI CGANNNNNNTGC 2 cut(s) 247, 281
BciT130I CCWGG 1 cut(s) 206
BcnI CCSGG 3 cut(s) 534, 666, 667
BcoDI GTCTC 1 cut(s) 305
BfmI CTRYAG 1 cut(s) 630
BglII AGATCT 1 cut(s) 947
BisI GCNGC 3 cut(s) 245, 333, 616
BlsI GCNGC 3 cut(s) 246, 334, 617
Bme1390I CCNGG 4 cut(s) 206, 534, 666, 667
Bme18I GGWCC 4 cut(s) 209, 222, 291, 383
BmeT110I CYCGRG 1 cut(s) 665
BmgT120I GGNCC 7 cut(s) 180, 209, 222, 252, 291, 383, 439
BmiI GGNNCC 6 cut(s) 210, 211, 292, 293, 384, 562
BmrFI CCNGG 4 cut(s) 206, 534, 666, 667
BmrI ACTGGG 1 cut(s) 446
BmsI GCATC 4 cut(s) 360, 403, 472, 885
BmuI ACTGGG 1 cut(s) 446
BoxI GACNNNNGTC 1 cut(s) 214
Bpu10I CCTNAGC 2 cut(s) 248, 519
Bpu14I TTCGAA 1 cut(s) 394
BpuEI CTTGAG 3 cut(s) 392, 458, 704
BpuMI CCSGG 3 cut(s) 534, 666, 667
Bsa29I ATCGAT 1 cut(s) 779
BsaAI YACGTR 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 305
Bsc4I CCNNNNNNNGG 1 cut(s) 219
Bse118I RCCGGY 2 cut(s) 254, 347
Bse1I ACTGG 2 cut(s) 403, 441
BseBI CCWGG 1 cut(s) 206
BseCI ATCGAT 1 cut(s) 779
BseGI GGATG 4 cut(s) 351, 418, 529, 878
BseLI CCNNNNNNNGG 1 cut(s) 219
BseMII CTCAG 3 cut(s) 239, 340, 510
BseNI ACTGG 2 cut(s) 403, 441
BseXI GCAGC 2 cut(s) 231, 344
BsgI GTGCAG 1 cut(s) 184
Bsh1236I CGCG 2 cut(s) 215, 220
BshFI GGCC 3 cut(s) 181, 254, 441
BshNI GGYRCC 1 cut(s) 560
BshVI ATCGAT 1 cut(s) 779
BsiHKCI CYCGRG 1 cut(s) 665
BsiSI CCGG 4 cut(s) 255, 348, 533, 666
BslFI GGGAC 2 cut(s) 222, 277
BslI CCNNNNNNNGG 1 cut(s) 219
BsmAI GTCTC 1 cut(s) 305
BsmFI GGGAC 2 cut(s) 222, 277
BsmI GAATGC 2 cut(s) 813, 898
BsnI GGCC 3 cut(s) 181, 254, 441
Bso31I GGTCTC 1 cut(s) 305
BsoBI CYCGRG 1 cut(s) 665
Bsp119I TTCGAA 1 cut(s) 394
Bsp143I GATC 8 cut(s) 172, 192, 447, 679, 776, 780, 918, 947
Bsp19I CCATGG 3 cut(s) 231, 286, 336
BspACI CCGC 4 cut(s) 213, 220, 271, 616
BspANI GGCC 3 cut(s) 181, 254, 441
BspCNI CTCAG 3 cut(s) 240, 339, 511
BspDI ATCGAT 1 cut(s) 779
BspFNI CGCG 2 cut(s) 215, 220
BspLI GGNNCC 6 cut(s) 210, 211, 292, 293, 384, 562
BspMAI CTGCAG 1 cut(s) 634
BspPI GGATC 3 cut(s) 180, 687, 784
BspT104I TTCGAA 1 cut(s) 394
BspT107I GGYRCC 1 cut(s) 560
BspTNI GGTCTC 1 cut(s) 305
BsrFI RCCGGY 2 cut(s) 254, 347
BsrI ACTGG 2 cut(s) 403, 441
BssAI RCCGGY 2 cut(s) 254, 347
BssMI GATC 8 cut(s) 172, 192, 447, 679, 776, 780, 918, 947
BssSI CACGAG 2 cut(s) 296, 911
BssT1I CCWWGG 5 cut(s) 231, 286, 336, 378, 804
Bst2BI CACGAG 2 cut(s) 296, 911
Bst2UI CCWGG 1 cut(s) 206
BstAPI GCANNNNNTGC 1 cut(s) 357
BstBAI YACGTR 1 cut(s) 42
BstBI TTCGAA 1 cut(s) 394
BstC8I GCNNGC 3 cut(s) 256, 271, 516
BstDEI CTNAG 5 cut(s) 248, 326, 519, 818, 959
BstDSI CCRYGG 3 cut(s) 231, 286, 336
BstF5I GGATG 4 cut(s) 351, 418, 529, 878
BstFNI CGCG 2 cut(s) 215, 220
BstHHI GCGC 1 cut(s) 362
BstKTI GATC 8 cut(s) 175, 195, 450, 682, 779, 783, 921, 950
BstMAI GTCTC 1 cut(s) 305
BstMBI GATC 8 cut(s) 172, 192, 447, 679, 776, 780, 918, 947
BstMWI GCNNNNNNNGC 1 cut(s) 357
BstNI CCWGG 1 cut(s) 206
BstPAI GACNNNNGTC 1 cut(s) 214
BstSCI CCNGG 4 cut(s) 204, 532, 664, 665
BstSFI CTRYAG 1 cut(s) 630
BstUI CGCG 2 cut(s) 215, 220
BstV1I GCAGC 2 cut(s) 231, 344
BstX2I RGATCY 2 cut(s) 679, 947
BstYI RGATCY 2 cut(s) 679, 947
Bsu15I ATCGAT 1 cut(s) 779
BsuRI GGCC 3 cut(s) 181, 254, 441
BsuTUI ATCGAT 1 cut(s) 779
BtgI CCRYGG 3 cut(s) 231, 286, 336
BtgZI GCGATG 1 cut(s) 48
BtsCI GGATG 4 cut(s) 351, 418, 529, 878
BtsIMutI CAGTG 1 cut(s) 459
Cac8I GCNNGC 3 cut(s) 256, 271, 516
CfoI GCGC 1 cut(s) 362
Cfr10I RCCGGY 2 cut(s) 254, 347
Cfr13I GGNCC 7 cut(s) 180, 209, 222, 252, 291, 383, 439
Cfr9I CCCGGG 1 cut(s) 665
ClaI ATCGAT 1 cut(s) 779
CpoI CGGWCCG 1 cut(s) 222
CseI GACGC 1 cut(s) 204
Csp6I GTAC 2 cut(s) 60, 301
CspI CGGWCCG 1 cut(s) 222
CviAII CATG 9 cut(s) 232, 259, 287, 337, 367, 541, 748, 834, 903
CviQI GTAC 2 cut(s) 60, 301
DdeI CTNAG 5 cut(s) 248, 326, 519, 818, 959
DpnI GATC 8 cut(s) 174, 194, 449, 681, 778, 782, 920, 949
DpnII GATC 8 cut(s) 172, 192, 447, 679, 776, 780, 918, 947
DraIII CACNNNGTG 1 cut(s) 916
Eco130I CCWWGG 5 cut(s) 231, 286, 336, 378, 804
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 4 cut(s) 209, 222, 291, 383
Eco72I CACGTG 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 665
EcoO109I RGGNCCY 2 cut(s) 209, 291
EcoRII CCWGG 1 cut(s) 204
EcoT14I CCWWGG 5 cut(s) 231, 286, 336, 378, 804
EcoT22I ATGCAT 1 cut(s) 900
ErhI CCWWGG 5 cut(s) 231, 286, 336, 378, 804
FaeI CATG 9 cut(s) 235, 262, 290, 340, 370, 544, 751, 837, 906
FaqI GGGAC 2 cut(s) 222, 277
FatI CATG 9 cut(s) 231, 258, 286, 336, 366, 540, 747, 833, 902
FauI CCCGC 2 cut(s) 220, 278
Fnu4HI GCNGC 3 cut(s) 245, 333, 616
FokI GGATG 4 cut(s) 338, 425, 536, 865
Fsp4HI GCNGC 3 cut(s) 245, 333, 616
GlaI GCGC 1 cut(s) 361
GluI GCNGC 3 cut(s) 245, 333, 616
HaeIII GGCC 3 cut(s) 181, 254, 441
HapII CCGG 4 cut(s) 255, 348, 533, 666
HgaI GACGC 1 cut(s) 204
HhaI GCGC 1 cut(s) 362
Hin1II CATG 9 cut(s) 235, 262, 290, 340, 370, 544, 751, 837, 906
Hin6I GCGC 1 cut(s) 360
HinP1I GCGC 1 cut(s) 360
HinfI GANTC 5 cut(s) 32, 341, 391, 544, 849
HpaII CCGG 4 cut(s) 255, 348, 533, 666
HphI GGTGA 4 cut(s) 442, 491, 653, 928
Hpy188I TCNGA 5 cut(s) 160, 177, 771, 894, 962
Hpy188III TCNNGA 4 cut(s) 308, 386, 795, 853
Hpy99I CGWCG 2 cut(s) 220, 269
HpyAV CCTTC 2 cut(s) 838, 850
HpyCH4IV ACGT 3 cut(s) 41, 264, 654
HpyCH4V TGCA 5 cut(s) 201, 485, 632, 886, 898
HpyF10VI GCNNNNNNNGC 1 cut(s) 357
HpyF3I CTNAG 5 cut(s) 248, 326, 519, 818, 959
HpySE526I ACGT 3 cut(s) 41, 264, 654
Hsp92II CATG 9 cut(s) 235, 262, 290, 340, 370, 544, 751, 837, 906
HspAI GCGC 1 cut(s) 360
KflI GGGWCCC 2 cut(s) 209, 291
KroI GCCGGC 1 cut(s) 254
KroNI GCCGGC 1 cut(s) 256
Kzo9I GATC 8 cut(s) 172, 192, 447, 679, 776, 780, 918, 947
LmnI GCTCC 2 cut(s) 537, 806
Lsp1109I GCAGC 2 cut(s) 231, 344
LweI GCATC 4 cut(s) 360, 403, 472, 885
MaeII ACGT 3 cut(s) 41, 264, 654
MaeIII GTNAC 1 cut(s) 503
MalI GATC 8 cut(s) 174, 194, 449, 681, 778, 782, 920, 949
MboI GATC 8 cut(s) 172, 192, 447, 679, 776, 780, 918, 947
MboII GAAGA 4 cut(s) 653, 661, 722, 942
MfeI CAATTG 1 cut(s) 938
MflI RGATCY 2 cut(s) 679, 947
MluCI AATT 3 cut(s) 5, 932, 938
MlyI GAGTC 1 cut(s) 41
MnlI CCTC 9 cut(s) 154, 171, 243, 326, 482, 514, 639, 783, 907
Mph1103I ATGCAT 1 cut(s) 900
MroNI GCCGGC 1 cut(s) 254
MroXI GAANNNNTTC 2 cut(s) 714, 813
MseI TTAA 2 cut(s) 657, 692
MslI CAYNNNNRTG 1 cut(s) 40
MspA1I CMGCKG 1 cut(s) 618
MspI CCGG 4 cut(s) 255, 348, 533, 666
MspR9I CCNGG 4 cut(s) 206, 534, 666, 667
MunI CAATTG 1 cut(s) 938
Mva1269I GAATGC 2 cut(s) 813, 898
MvaI CCWGG 1 cut(s) 206
MvnI CGCG 2 cut(s) 215, 220
MwoI GCNNNNNNNGC 1 cut(s) 357
NaeI GCCGGC 1 cut(s) 256
NciI CCSGG 3 cut(s) 534, 666, 667
NcoI CCATGG 3 cut(s) 231, 286, 336
NdeII GATC 8 cut(s) 172, 192, 447, 679, 776, 780, 918, 947
NgoMIV GCCGGC 1 cut(s) 254
NlaIII CATG 9 cut(s) 235, 262, 290, 340, 370, 544, 751, 837, 906
NlaIV GGNNCC 6 cut(s) 210, 211, 292, 293, 384, 562
NsiI ATGCAT 1 cut(s) 900
NspV TTCGAA 1 cut(s) 394
PasI CCCWGGG 1 cut(s) 205
PctI GAATGC 2 cut(s) 813, 898
PdiI GCCGGC 1 cut(s) 256
PdmI GAANNNNTTC 2 cut(s) 714, 813
PfeI GAWTC 4 cut(s) 341, 391, 544, 849
PkrI GCNGC 3 cut(s) 246, 334, 617
PleI GAGTC 1 cut(s) 40
PmaCI CACGTG 1 cut(s) 42
PmlI CACGTG 1 cut(s) 42
PpsI GAGTC 1 cut(s) 40
Ppu21I YACGTR 1 cut(s) 42
PpuMI RGGWCCY 2 cut(s) 209, 291
PshAI GACNNNNGTC 1 cut(s) 214
Psp1406I AACGTT 1 cut(s) 654
Psp5II RGGWCCY 2 cut(s) 209, 291
Psp6I CCWGG 1 cut(s) 204
PspCI CACGTG 1 cut(s) 42
PspGI CCWGG 1 cut(s) 204
PspN4I GGNNCC 6 cut(s) 210, 211, 292, 293, 384, 562
PspPI GGNCC 7 cut(s) 180, 209, 222, 252, 291, 383, 439
PspPPI RGGWCCY 2 cut(s) 209, 291
PstI CTGCAG 1 cut(s) 634
PsuI RGATCY 2 cut(s) 679, 947
RsaI GTAC 2 cut(s) 61, 302
RsaNI GTAC 2 cut(s) 60, 301
RseI CAYNNNNRTG 1 cut(s) 40
Rsr2I CGGWCCG 1 cut(s) 222
RsrII CGGWCCG 1 cut(s) 222
SaqAI TTAA 2 cut(s) 657, 692
SatI GCNGC 3 cut(s) 245, 333, 616
Sau3AI GATC 8 cut(s) 172, 192, 447, 679, 776, 780, 918, 947
Sau96I GGNCC 7 cut(s) 180, 209, 222, 252, 291, 383, 439
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 4 cut(s) 206, 534, 666, 667
SfaNI GCATC 4 cut(s) 360, 403, 472, 885
SfcI CTRYAG 1 cut(s) 630
SfuI TTCGAA 1 cut(s) 394
SinI GGWCC 4 cut(s) 209, 222, 291, 383
SmaI CCCGGG 1 cut(s) 667
SmiMI CAYNNNNRTG 1 cut(s) 40
SmlI CTYRAG 3 cut(s) 407, 473, 719
SmoI CTYRAG 3 cut(s) 407, 473, 719
Sse9I AATT 3 cut(s) 5, 932, 938
SsiI CCGC 4 cut(s) 213, 220, 271, 616
StyD4I CCNGG 4 cut(s) 204, 532, 664, 665
StyI CCWWGG 5 cut(s) 231, 286, 336, 378, 804
TaiI ACGT 3 cut(s) 44, 267, 657
TaqI TCGA 4 cut(s) 318, 372, 394, 779
TasI AATT 3 cut(s) 5, 932, 938
TatI WGTACW 1 cut(s) 59
TauI GCSGC 1 cut(s) 618
TfiI GAWTC 4 cut(s) 341, 391, 544, 849
Tru1I TTAA 2 cut(s) 657, 692
Tru9I TTAA 2 cut(s) 657, 692
TscAI CASTG 1 cut(s) 466
TseI GCWGC 2 cut(s) 244, 332
TspDTI ATGAA 3 cut(s) 17, 275, 891
TspGWI ACGGA 1 cut(s) 214
TspMI CCCGGG 1 cut(s) 665
TspRI CASTG 1 cut(s) 466
VpaK11BI GGWCC 4 cut(s) 209, 222, 291, 383
XapI RAATTY 2 cut(s) 5, 932
XcmI CCANNNNNNNNNTGG 1 cut(s) 571
XmaI CCCGGG 1 cut(s) 665
XmnI GAANNNNTTC 2 cut(s) 714, 813
Zsp2I ATGCAT 1 cut(s) 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.