MD11G1002500.v1.1

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0028892
Physical Location & Seq
Reverse (-)
226697 .. 228558
1862 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1002500.v1.1.491

Sequence Viewer

Length: 846 bp
ATGCAGTTGGGCGTTAGTGTGGTGTTCTCTGCAGGTAATGAAGGGCCAGACCCCTCACAGGTCACAAACGTTGCTCCTTGGGGAATTTGTGTAGCTGCTTCCTCCGTTGATCGAATGTTTCCAACCCGAATAATCCTATACAATAAGCTCCTTGCCAGGGAATTGGATAAAATCAAAGTTTGCGGTTCGAAGAGTAATTCTATGCTTCTCAAGTATAGGACCATTGAGGCAAGCAAAAGCAGCTGCCAAGAAAGCGAATGCATCGGGTTTGATCTTTGTGGAATCTGTGACAAGGCAGGTTGCCGTCGACATCATCCCCTCAGTCCATGTCAATCTTCCATCTGGAATTTTCCGTCGGGAACATTAATGTCATGCCCTCATGTGTCCGGAGTCGTTGCCCTTATCAAATCTGCACATCCGAATTGGTCTCCCGCAGTCATTAGGTCTACTCTCATCACCATAGCCATTGATCATCATTCATGTTCAATTGTCACAACAAACACGAGGGACACATCCTTTGACAGCATTCTAGCGGATGGATCAACGAAAGCCTCCATCCCCTTGACATTGGTGCCGGCCACATTGATTGACCCCATCAAACCGATGGATCCAGGCCTCGTTTACGGCATGAAAACAAGCGAATACGTTCTATTCCTCTGCAACATTGGCTACACCGGTGACCAAATCAATCAGATTTTCTTTGCTCTCCCGGAACTGACACAAGCTGTCCACAAGTACCAACTTCAAATTTTCAGCATAAAATACGCCTCCATCACAGTTTCCAATTGCCAATCTCCAATCCACCGTGACGATCAAAAAAAGCGTCCGCAACGTGGGGAAAACTAA

Protein Analysis

282

Amino Acids

30.72

Weight (kDa)

8.42

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 2 - 166 9.6e-12 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0028892)

Species Orthologous Gene IDs
malus_domestica MD11G1002500.v1.1
pyrus_communis pycom11g00230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 23, 287
AccB1I GGYRCC 1 cut(s) 571
AccI GTMKAC 2 cut(s) 307, 446
AccIII TCCGGA 1 cut(s) 386
AciI CCGC 4 cut(s) 183, 432, 533, 827
AclI AACGTT 1 cut(s) 69
AclWI GGATC 3 cut(s) 547, 602, 615
AcoI YGGCCR 1 cut(s) 576
AcsI RAATTY 3 cut(s) 84, 346, 747
AfaI GTAC 1 cut(s) 737
AfiI CCNNNNNNNGG 3 cut(s) 58, 157, 833
AgeI ACCGGT 1 cut(s) 674
AgsI TTSAA 2 cut(s) 486, 746
AjnI CCWGG 2 cut(s) 155, 610
AluBI AGCT 4 cut(s) 95, 148, 243, 725
AluI AGCT 4 cut(s) 95, 148, 243, 725
Alw26I GTCTC 1 cut(s) 432
AlwI GGATC 3 cut(s) 547, 602, 615
Aor13HI TCCGGA 1 cut(s) 386
AoxI GGCC 3 cut(s) 44, 576, 613
ApeKI GCWGC 3 cut(s) 95, 240, 243
ApoI RAATTY 3 cut(s) 84, 346, 747
ArsI GACNNNNNNTTYG 4 cut(s) 500, 532, 808, 840
AseI ATTAAT 1 cut(s) 365
AsiGI ACCGGT 1 cut(s) 674
Asp700I GAANNNNTTC 1 cut(s) 645
AspS9I GGNCC 2 cut(s) 44, 219
AsuC2I CCSGG 1 cut(s) 710
AsuHPI GGTGA 2 cut(s) 448, 689
AsuII TTCGAA 1 cut(s) 188
AvaII GGWCC 1 cut(s) 219
BamHI GGATCC 1 cut(s) 607
BanI GGYRCC 1 cut(s) 571
BauI CACGAG 1 cut(s) 502
BbvI GCAGC 3 cut(s) 82, 230, 252
BccI CCATC 6 cut(s) 347, 530, 563, 598, 602, 779
BceAI ACGGC 2 cut(s) 288, 640
BciT130I CCWGG 2 cut(s) 157, 612
BclI TGATCA 1 cut(s) 469
BcnI CCSGG 1 cut(s) 710
BcoDI GTCTC 1 cut(s) 432
BfaI CTAG 1 cut(s) 530
BfmI CTRYAG 1 cut(s) 30
BfuAI ACCTGC 2 cut(s) 23, 287
BisI GCNGC 3 cut(s) 96, 241, 244
BlsI GCNGC 3 cut(s) 97, 242, 245
Bme1390I CCNGG 3 cut(s) 157, 612, 710
Bme18I GGWCC 1 cut(s) 219
BmgT120I GGNCC 2 cut(s) 44, 219
BmiI GGNNCC 2 cut(s) 573, 609
BmrFI CCNGG 3 cut(s) 157, 612, 710
BmsI GCATC 1 cut(s) 270
Bpu14I TTCGAA 1 cut(s) 188
BpuEI CTTGAG 1 cut(s) 194
BpuMI CCSGG 1 cut(s) 710
BsaI GGTCTC 1 cut(s) 432
BsaJI CCNNGG 2 cut(s) 77, 156
BsaWI WCCGGW 2 cut(s) 386, 674
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 157, 833
Bse118I RCCGGY 2 cut(s) 574, 674
BseAI TCCGGA 1 cut(s) 386
BseBI CCWGG 2 cut(s) 157, 612
BseDI CCNNGG 2 cut(s) 77, 156
BseGI GGATG 5 cut(s) 313, 415, 512, 541, 555
BseLI CCNNNNNNNGG 3 cut(s) 58, 157, 833
BseMII CTCAG 1 cut(s) 334
BseXI GCAGC 3 cut(s) 82, 230, 252
BsgI GTGCAG 1 cut(s) 396
BshFI GGCC 3 cut(s) 46, 578, 615
BshNI GGYRCC 1 cut(s) 571
BshTI ACCGGT 1 cut(s) 674
BsiSI CCGG 4 cut(s) 387, 575, 675, 710
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 3 cut(s) 58, 157, 833
BsmAI GTCTC 1 cut(s) 432
BsmFI GGGAC 1 cut(s) 521
BsmI GAATGC 2 cut(s) 263, 525
BsnI GGCC 3 cut(s) 46, 578, 615
Bso31I GGTCTC 1 cut(s) 432
Bsp119I TTCGAA 1 cut(s) 188
Bsp13I TCCGGA 1 cut(s) 386
Bsp143I GATC 6 cut(s) 109, 271, 469, 539, 607, 811
BspACI CCGC 4 cut(s) 183, 432, 533, 827
BspANI GGCC 3 cut(s) 46, 578, 615
BspCNI CTCAG 1 cut(s) 333
BspEI TCCGGA 1 cut(s) 386
BspLI GGNNCC 2 cut(s) 573, 609
BspMAI CTGCAG 1 cut(s) 34
BspMI ACCTGC 2 cut(s) 23, 287
BspPI GGATC 3 cut(s) 547, 602, 615
BspT104I TTCGAA 1 cut(s) 188
BspT107I GGYRCC 1 cut(s) 571
BspTNI GGTCTC 1 cut(s) 432
BsrFI RCCGGY 2 cut(s) 574, 674
BssAI RCCGGY 2 cut(s) 574, 674
BssECI CCNNGG 2 cut(s) 77, 156
BssMI GATC 6 cut(s) 109, 271, 469, 539, 607, 811
BssSI CACGAG 1 cut(s) 502
BssT1I CCWWGG 1 cut(s) 77
Bst2BI CACGAG 1 cut(s) 502
Bst2UI CCWGG 2 cut(s) 157, 612
Bst4CI ACNGT 2 cut(s) 778, 806
Bst6I CTCTTC 1 cut(s) 185
BstBI TTCGAA 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 232, 576
BstDEI CTNAG 1 cut(s) 320
BstEII GGTNACC 1 cut(s) 677
BstF5I GGATG 5 cut(s) 313, 415, 512, 541, 555
BstKTI GATC 6 cut(s) 112, 274, 472, 542, 610, 814
BstMAI GTCTC 1 cut(s) 432
BstMBI GATC 6 cut(s) 109, 271, 469, 539, 607, 811
BstMWI GCNNNNNNNGC 3 cut(s) 240, 252, 666
BstNI CCWGG 2 cut(s) 157, 612
BstPI GGTNACC 1 cut(s) 677
BstSCI CCNGG 3 cut(s) 155, 610, 708
BstSFI CTRYAG 1 cut(s) 30
BstV1I GCAGC 3 cut(s) 82, 230, 252
BstX2I RGATCY 1 cut(s) 607
BstXI CCANNNNNNTGG 1 cut(s) 163
BstYI RGATCY 1 cut(s) 607
BsuRI GGCC 3 cut(s) 46, 578, 615
BtsCI GGATG 5 cut(s) 313, 415, 512, 541, 555
BveI ACCTGC 2 cut(s) 23, 287
Cac8I GCNNGC 2 cut(s) 232, 576
Cfr10I RCCGGY 2 cut(s) 574, 674
Cfr13I GGNCC 2 cut(s) 44, 219
CseI GACGC 1 cut(s) 812
Csp6I GTAC 1 cut(s) 736
CspAI ACCGGT 1 cut(s) 674
CspCI CAANNNNNGTGG 2 cut(s) 568, 603
CviAII CATG 5 cut(s) 327, 372, 380, 480, 628
CviQI GTAC 1 cut(s) 736
DdeI CTNAG 1 cut(s) 320
DpnI GATC 6 cut(s) 111, 273, 471, 541, 609, 813
DpnII GATC 6 cut(s) 109, 271, 469, 539, 607, 811
EaeI YGGCCR 1 cut(s) 576
Eam1104I CTCTTC 1 cut(s) 185
EarI CTCTTC 1 cut(s) 185
Eco130I CCWWGG 1 cut(s) 77
Eco147I AGGCCT 1 cut(s) 615
Eco31I GGTCTC 1 cut(s) 432
Eco47I GGWCC 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 677
EcoO65I GGTNACC 1 cut(s) 677
EcoRII CCWGG 2 cut(s) 155, 610
EcoT14I CCWWGG 1 cut(s) 77
EcoT22I ATGCAT 1 cut(s) 263
ErhI CCWWGG 1 cut(s) 77
FaeI CATG 5 cut(s) 330, 375, 383, 483, 631
FaqI GGGAC 1 cut(s) 521
FatI CATG 5 cut(s) 326, 371, 379, 479, 627
FauI CCCGC 1 cut(s) 439
FbaI TGATCA 1 cut(s) 469
FblI GTMKAC 2 cut(s) 307, 446
Fnu4HI GCNGC 3 cut(s) 96, 241, 244
FokI GGATG 5 cut(s) 300, 402, 499, 542, 548
Fsp4HI GCNGC 3 cut(s) 96, 241, 244
FspBI CTAG 1 cut(s) 530
GluI GCNGC 3 cut(s) 96, 241, 244
HaeIII GGCC 3 cut(s) 46, 578, 615
HapII CCGG 4 cut(s) 387, 575, 675, 710
HgaI GACGC 1 cut(s) 812
Hin1II CATG 5 cut(s) 330, 375, 383, 483, 631
HincII GTYRAC 1 cut(s) 308
HindII GTYRAC 1 cut(s) 308
HinfI GANTC 2 cut(s) 282, 390
HpaII CCGG 4 cut(s) 387, 575, 675, 710
HphI GGTGA 2 cut(s) 448, 689
Hpy166II GTNNAC 4 cut(s) 308, 447, 622, 730
Hpy188I TCNGA 2 cut(s) 420, 693
Hpy188III TCNNGA 3 cut(s) 343, 357, 387
Hpy8I GTNNAC 4 cut(s) 308, 447, 622, 730
Hpy99I CGWCG 2 cut(s) 309, 358
HpyAV CCTTC 1 cut(s) 35
HpyCH4III ACNGT 2 cut(s) 778, 806
HpyCH4IV ACGT 3 cut(s) 69, 645, 832
HpyCH4V TGCA 5 cut(s) 4, 32, 261, 413, 660
HpyF10VI GCNNNNNNNGC 3 cut(s) 240, 252, 666
HpyF3I CTNAG 1 cut(s) 320
HpySE526I ACGT 3 cut(s) 69, 645, 832
Hsp92II CATG 5 cut(s) 330, 375, 383, 483, 631
Kpn2I TCCGGA 1 cut(s) 386
KroI GCCGGC 1 cut(s) 574
KroNI GCCGGC 1 cut(s) 576
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 6 cut(s) 109, 271, 469, 539, 607, 811
LmnI GCTCC 2 cut(s) 79, 153
Lsp1109I GCAGC 3 cut(s) 82, 230, 252
LweI GCATC 1 cut(s) 270
MaeI CTAG 1 cut(s) 530
MaeII ACGT 3 cut(s) 69, 645, 832
MaeIII GTNAC 5 cut(s) 61, 287, 490, 677, 806
MalI GATC 6 cut(s) 111, 273, 471, 541, 609, 813
MboI GATC 6 cut(s) 109, 271, 469, 539, 607, 811
MboII GAAGA 2 cut(s) 202, 327
MfeI CAATTG 2 cut(s) 486, 784
MflI RGATCY 1 cut(s) 607
MluCI AATT 8 cut(s) 84, 161, 196, 346, 421, 486, 747, 784
MlyI GAGTC 1 cut(s) 399
MmeI TCCRAC 1 cut(s) 146
Mph1103I ATGCAT 1 cut(s) 263
MroI TCCGGA 1 cut(s) 386
MroNI GCCGGC 1 cut(s) 574
MroXI GAANNNNTTC 1 cut(s) 645
MseI TTAA 1 cut(s) 365
MspA1I CMGCKG 1 cut(s) 243
MspI CCGG 4 cut(s) 387, 575, 675, 710
MspR9I CCNGG 3 cut(s) 157, 612, 710
MunI CAATTG 2 cut(s) 486, 784
Mva1269I GAATGC 2 cut(s) 263, 525
MvaI CCWGG 2 cut(s) 157, 612
MwoI GCNNNNNNNGC 3 cut(s) 240, 252, 666
NaeI GCCGGC 1 cut(s) 576
NciI CCSGG 1 cut(s) 710
NdeII GATC 6 cut(s) 109, 271, 469, 539, 607, 811
NgoMIV GCCGGC 1 cut(s) 574
NlaIII CATG 5 cut(s) 330, 375, 383, 483, 631
NlaIV GGNNCC 2 cut(s) 573, 609
NmuCI GTSAC 5 cut(s) 61, 287, 490, 677, 806
NsiI ATGCAT 1 cut(s) 263
NspV TTCGAA 1 cut(s) 188
PceI AGGCCT 1 cut(s) 615
PctI GAATGC 2 cut(s) 263, 525
PdiI GCCGGC 1 cut(s) 576
PdmI GAANNNNTTC 1 cut(s) 645
PfeI GAWTC 1 cut(s) 282
PfoI TCCNGGA 1 cut(s) 708
PinAI ACCGGT 1 cut(s) 674
PkrI GCNGC 3 cut(s) 97, 242, 245
PleI GAGTC 1 cut(s) 398
PpsI GAGTC 1 cut(s) 398
PshBI ATTAAT 1 cut(s) 365
Psp1406I AACGTT 1 cut(s) 69
Psp6I CCWGG 2 cut(s) 155, 610
PspEI GGTNACC 1 cut(s) 677
PspGI CCWGG 2 cut(s) 155, 610
PspN4I GGNNCC 2 cut(s) 573, 609
PspPI GGNCC 2 cut(s) 44, 219
PstI CTGCAG 1 cut(s) 34
PsuI RGATCY 1 cut(s) 607
PvuII CAGCTG 1 cut(s) 243
RsaI GTAC 1 cut(s) 737
RsaNI GTAC 1 cut(s) 736
SalI GTCGAC 1 cut(s) 306
SaqAI TTAA 1 cut(s) 365
SatI GCNGC 3 cut(s) 96, 241, 244
Sau3AI GATC 6 cut(s) 109, 271, 469, 539, 607, 811
Sau96I GGNCC 2 cut(s) 44, 219
SchI GAGTC 1 cut(s) 399
ScrFI CCNGG 3 cut(s) 157, 612, 710
SfaNI GCATC 1 cut(s) 270
SfcI CTRYAG 1 cut(s) 30
SfuI TTCGAA 1 cut(s) 188
SgrAI CRCCGGYG 1 cut(s) 674
SinI GGWCC 1 cut(s) 219
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 8 cut(s) 84, 161, 196, 346, 421, 486, 747, 784
SseBI AGGCCT 1 cut(s) 615
SsiI CCGC 4 cut(s) 183, 432, 533, 827
SspMI CTAG 1 cut(s) 530
StuI AGGCCT 1 cut(s) 615
StyD4I CCNGG 3 cut(s) 155, 610, 708
StyI CCWWGG 1 cut(s) 77
TaaI ACNGT 2 cut(s) 778, 806
TaiI ACGT 3 cut(s) 72, 648, 835
TaqI TCGA 3 cut(s) 112, 188, 307
TasI AATT 8 cut(s) 84, 161, 196, 346, 421, 486, 747, 784
TfiI GAWTC 1 cut(s) 282
Tru1I TTAA 1 cut(s) 365
Tru9I TTAA 1 cut(s) 365
TseFI GTSAC 5 cut(s) 61, 287, 490, 677, 806
TseI GCWGC 3 cut(s) 95, 240, 243
Tsp45I GTSAC 5 cut(s) 61, 287, 490, 677, 806
TspDTI ATGAA 3 cut(s) 54, 468, 644
TspGWI ACGGA 2 cut(s) 94, 342
VpaK11BI GGWCC 1 cut(s) 219
VspI ATTAAT 1 cut(s) 365
XapI RAATTY 3 cut(s) 84, 346, 747
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XmiI GTMKAC 2 cut(s) 307, 446
XmnI GAANNNNTTC 1 cut(s) 645
XspI CTAG 1 cut(s) 530
Zsp2I ATGCAT 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.