MD11G1052000.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0019555
Physical Location & Seq
Forward (+)
4440914 .. 4443960
3047 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1052000.v1.1.491

Sequence Viewer

Length: 447 bp
ATGGCATCTAAACGCATCTTGAAGGAGCTCAAGGATTTGCAGAAGGACCCCCCTACCTCATGCAGTGCTGGTCCTGTGGCCGAGGACATGTTCCATTGGCAAGCGACAATTATGGGACCTGGTGATAGTCCATACACGGGTGGTGTTTTTCTAGTTTCAATTCATTTCCCTCCAGATTATCCATTCAAGCCTCCAAAGGTTGCATTTAGGACCAAGGTATTCCACCCAAACATTAATAGCAATGGAAGCATATGTCTTGATATTCTCAAAGAACAATGGAGCCCGGCACTAACCATTTCCAAGGTGCTGCTGTCCATATGCTCTCTGTTGACGGACCCCAACCCCGACGACCCGCTTGTCCCCGAAATTGCACACATGTACAAGACGGACAGGGCAAAGTATGAAGCCACTGCTCGCAGTTGGACTCAGAAGTACGCGATGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

149

Amino Acids

16.48

Weight (kDa)

7.72

Isoelectric Point (pI)

43.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 5 - 141 2.5e-54 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41700 AT5G41700 AT5G41700 AT5G41700
malus_domestica MD11G1052000.v1.1
pyrus_communis pycom03g03940 pycom11g04240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 356
AccII CGCG 1 cut(s) 437
AciI CCGC 1 cut(s) 353
AcoI YGGCCR 1 cut(s) 78
AfaI GTAC 2 cut(s) 380, 434
AfiI CCNNNNNNNGG 1 cut(s) 137
AflIII ACRYGT 2 cut(s) 87, 375
AgsI TTSAA 3 cut(s) 22, 159, 187
AjnI CCWGG 1 cut(s) 118
AluBI AGCT 1 cut(s) 28
AluI AGCT 1 cut(s) 28
Alw21I GWGCWC 1 cut(s) 30
AoxI GGCC 1 cut(s) 78
ApeKI GCWGC 1 cut(s) 307
AseI ATTAAT 1 cut(s) 234
AspS9I GGNCC 5 cut(s) 46, 71, 116, 210, 334
AsuC2I CCSGG 1 cut(s) 284
AsuHPI GGTGA 1 cut(s) 134
AvaII GGWCC 5 cut(s) 46, 71, 116, 210, 334
BanII GRGCYC 2 cut(s) 30, 284
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 1 cut(s) 294
BccI CCATC 1 cut(s) 433
BciT130I CCWGG 1 cut(s) 120
BcnI CCSGG 1 cut(s) 284
BfaI CTAG 1 cut(s) 152
BisI GCNGC 1 cut(s) 308
BlsI GCNGC 1 cut(s) 309
Bme1390I CCNGG 2 cut(s) 120, 284
Bme18I GGWCC 5 cut(s) 46, 71, 116, 210, 334
BmgT120I GGNCC 5 cut(s) 46, 71, 116, 210, 334
BmiI GGNNCC 4 cut(s) 48, 117, 281, 336
BmrFI CCNGG 2 cut(s) 120, 284
BmsI GCATC 2 cut(s) 14, 24
BpmI CTGGAG 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 14
BpuMI CCSGG 1 cut(s) 284
BsaJI CCNNGG 3 cut(s) 81, 213, 300
Bsc4I CCNNNNNNNGG 1 cut(s) 137
Bse3DI GCAATG 1 cut(s) 247
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 3 cut(s) 81, 213, 300
BseLI CCNNNNNNNGG 1 cut(s) 137
BseMI GCAATG 1 cut(s) 247
BseMII CTCAG 1 cut(s) 440
BseXI GCAGC 1 cut(s) 294
Bsh1236I CGCG 1 cut(s) 437
BshFI GGCC 1 cut(s) 80
BsiHKAI GWGCWC 1 cut(s) 30
BsiSI CCGG 1 cut(s) 284
BslFI GGGAC 2 cut(s) 129, 344
BslI CCNNNNNNNGG 1 cut(s) 137
BsmFI GGGAC 2 cut(s) 129, 344
BsnI GGCC 1 cut(s) 80
Bsp1286I GDGCHC 2 cut(s) 30, 284
Bsp1407I TGTACA 1 cut(s) 378
BspACI CCGC 1 cut(s) 353
BspANI GGCC 1 cut(s) 80
BspCNI CTCAG 1 cut(s) 439
BspFNI CGCG 1 cut(s) 437
BspLI GGNNCC 4 cut(s) 48, 117, 281, 336
BsrDI GCAATG 1 cut(s) 247
BsrGI TGTACA 1 cut(s) 378
BssECI CCNNGG 3 cut(s) 81, 213, 300
BssT1I CCWWGG 2 cut(s) 213, 300
Bst2UI CCWGG 1 cut(s) 120
BstAUI TGTACA 1 cut(s) 378
BstC8I GCNNGC 2 cut(s) 102, 415
BstDEI CTNAG 1 cut(s) 426
BstFNI CGCG 1 cut(s) 437
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstNI CCWGG 1 cut(s) 120
BstNSI RCATGY 2 cut(s) 91, 379
BstSCI CCNGG 2 cut(s) 118, 282
BstUI CGCG 1 cut(s) 437
BstV1I GCAGC 1 cut(s) 294
BsuRI GGCC 1 cut(s) 80
BtsI GCAGTG 2 cut(s) 70, 408
BtsIMutI CAGTG 2 cut(s) 70, 408
Cac8I GCNNGC 2 cut(s) 102, 415
Cfr13I GGNCC 5 cut(s) 46, 71, 116, 210, 334
CsiI ACCWGGT 1 cut(s) 118
Csp6I GTAC 2 cut(s) 379, 433
CviAII CATG 3 cut(s) 60, 88, 376
CviJI RGCY 5 cut(s) 28, 80, 190, 282, 407
CviKI_1 RGCY 5 cut(s) 28, 80, 190, 282, 407
CviQI GTAC 2 cut(s) 379, 433
DdeI CTNAG 1 cut(s) 426
DrdI GACNNNNNNGTC 1 cut(s) 356
DseDI GACNNNNNNGTC 1 cut(s) 356
EaeI YGGCCR 1 cut(s) 78
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 2 cut(s) 213, 300
Eco24I GRGCYC 2 cut(s) 30, 284
Eco47I GGWCC 5 cut(s) 46, 71, 116, 210, 334
Eco53kI GAGCTC 1 cut(s) 28
EcoICRI GAGCTC 1 cut(s) 28
EcoO109I RGGNCCY 2 cut(s) 46, 116
EcoRII CCWGG 1 cut(s) 118
EcoT14I CCWWGG 2 cut(s) 213, 300
EcoT38I GRGCYC 2 cut(s) 30, 284
ErhI CCWWGG 2 cut(s) 213, 300
FaeI CATG 3 cut(s) 63, 91, 379
FaqI GGGAC 2 cut(s) 129, 344
FatI CATG 3 cut(s) 59, 87, 375
FauI CCCGC 1 cut(s) 360
FauNDI CATATG 2 cut(s) 251, 317
Fnu4HI GCNGC 1 cut(s) 308
FriOI GRGCYC 2 cut(s) 30, 284
Fsp4HI GCNGC 1 cut(s) 308
FspBI CTAG 1 cut(s) 152
GluI GCNGC 1 cut(s) 308
GsuI CTGGAG 1 cut(s) 156
HaeIII GGCC 1 cut(s) 80
HapII CCGG 1 cut(s) 284
Hin1II CATG 3 cut(s) 63, 91, 379
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 1 cut(s) 424
HpaII CCGG 1 cut(s) 284
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 1 cut(s) 429
Hpy188III TCNNGA 3 cut(s) 19, 173, 257
Hpy8I GTNNAC 1 cut(s) 330
Hpy99I CGWCG 1 cut(s) 350
HpyAV CCTTC 2 cut(s) 16, 37
HpyCH4V TGCA 4 cut(s) 40, 63, 203, 371
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
HpyF3I CTNAG 1 cut(s) 426
Hsp92II CATG 3 cut(s) 63, 91, 379
LmnI GCTCC 2 cut(s) 25, 279
LpnPI CCDG 7 cut(s) 54, 87, 105, 132, 186, 297, 376
Lsp1109I GCAGC 1 cut(s) 294
LweI GCATC 2 cut(s) 14, 24
MabI ACCWGGT 1 cut(s) 118
MaeI CTAG 1 cut(s) 152
MhlI GDGCHC 2 cut(s) 30, 284
MluCI AATT 3 cut(s) 108, 159, 366
MlyI GAGTC 1 cut(s) 418
MmeI TCCRAC 1 cut(s) 401
MnlI CCTC 4 cut(s) 67, 76, 180, 201
MseI TTAA 1 cut(s) 234
MspI CCGG 1 cut(s) 284
MspR9I CCNGG 2 cut(s) 120, 284
MvaI CCWGG 1 cut(s) 120
MvnI CGCG 1 cut(s) 437
MwoI GCNNNNNNNGC 1 cut(s) 246
NciI CCSGG 1 cut(s) 284
NdeI CATATG 2 cut(s) 251, 317
NlaIII CATG 3 cut(s) 63, 91, 379
NlaIV GGNNCC 4 cut(s) 48, 117, 281, 336
NmeAIII GCCGAG 1 cut(s) 106
NspI RCATGY 2 cut(s) 91, 379
PciI ACATGT 2 cut(s) 87, 375
PkrI GCNGC 1 cut(s) 309
PleI GAGTC 1 cut(s) 418
PpsI GAGTC 1 cut(s) 418
PpuMI RGGWCCY 2 cut(s) 46, 116
PscI ACATGT 2 cut(s) 87, 375
PshBI ATTAAT 1 cut(s) 234
Psp124BI GAGCTC 1 cut(s) 30
Psp5II RGGWCCY 2 cut(s) 46, 116
Psp6I CCWGG 1 cut(s) 118
PspGI CCWGG 1 cut(s) 118
PspN4I GGNNCC 4 cut(s) 48, 117, 281, 336
PspPI GGNCC 5 cut(s) 46, 71, 116, 210, 334
PspPPI RGGWCCY 2 cut(s) 46, 116
RsaI GTAC 2 cut(s) 380, 434
RsaNI GTAC 2 cut(s) 379, 433
SacI GAGCTC 1 cut(s) 30
SaqAI TTAA 1 cut(s) 234
SatI GCNGC 1 cut(s) 308
Sau96I GGNCC 5 cut(s) 46, 71, 116, 210, 334
SchI GAGTC 1 cut(s) 418
ScrFI CCNGG 2 cut(s) 120, 284
SduI GDGCHC 2 cut(s) 30, 284
SetI ASST 6 cut(s) 30, 59, 121, 201, 219, 306
SexAI ACCWGGT 1 cut(s) 118
SfaNI GCATC 2 cut(s) 14, 24
SinI GGWCC 5 cut(s) 46, 71, 116, 210, 334
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 3 cut(s) 108, 159, 366
SsiI CCGC 1 cut(s) 353
SspMI CTAG 1 cut(s) 152
SstI GAGCTC 1 cut(s) 30
StyD4I CCNGG 2 cut(s) 118, 282
StyI CCWWGG 2 cut(s) 213, 300
TasI AATT 3 cut(s) 108, 159, 366
TatI WGTACW 1 cut(s) 378
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TscAI CASTG 2 cut(s) 70, 415
TseI GCWGC 1 cut(s) 307
TspDTI ATGAA 2 cut(s) 152, 417
TspGWI ACGGA 2 cut(s) 347, 401
TspRI CASTG 2 cut(s) 70, 415
VpaK11BI GGWCC 5 cut(s) 46, 71, 116, 210, 334
VspI ATTAAT 1 cut(s) 234
XceI RCATGY 2 cut(s) 91, 379
XspI CTAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.