MD11G1059700.v1.1

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0017490
Physical Location & Seq
Forward (+)
5177154 .. 5179513
2360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1059700.v1.1.491

Sequence Viewer

Length: 1605 bp
ATGGAAGCCAACGTTGGCTTTCTCTCTTTGTGTTTGCTAGTCACCTTCATTCGTTTTCTGATCAAACGTTTTTGGCATCAAAGCCATAATAAAAACGATAGCAAAGTATTCAAGCCAGACTATCCACTTCCTCCCACTCCAAAATGCCTAAAACCATGGCCTATTGTCGGCAACCTCCCCGAATTGCTGGCTAACAAGCCCACATTCCGGTGGATACACGAGATGATGGACGAAATGAACACCGAAATTACATGCATCCGTCTTGGGAACACGAATGTGATTCCCATCACTTGCCCTCAACTCAGCCGCGAAATCTTAAAGAAGCAAGACGCAATCTTCGCATCGCGTCCACTTAGCATTTCCACTTTTCTTATCACCAAGGGGTACAAAACTGCGGTTATGGTGCCCTTCGGAGAACAATGGAAGAAAATGAGGAAGGTAATAACGAGCGAATTGCTTACTCCAATGAGGCACAAGTGGCTCACGGACAAAAGGGTTGAAGAGGCCGATCACATCGTCAAGTATGTGTACAACCAGTGCAACAACAATGAAGGCGGTGGCATTGTAAATTTGAGATTTGCCACTCAACATTATTGTGCAAATGTGATTAAGAAAATGGTTCTCAACAAGAGGTACTTTGGGGAGGAAATGGAGGATGGTGGGCCTTGTTTGGAGGAACAAAAGTATTTAGAAAATGTATTTACAATGCTTAACTACATCTATTCATTTTCTGCATCCGATTACATTCCATGCTTGAGAGGGCTTGACTTGGACGGCCATGAGAAGATTATCAAGGATGCCATAAGAGATACAAGAAAATACCAAGACCCCTTAATCGAAGAGAGGGTTCGTGAACATCAGAAACTTGGCAATAACAAGGAGGCACACGACTTGCTTGACATTCTTATCTCTCTGAAAGATAAGAATGGCGAACCCTTACTGTCTCTGGAAGAAATCAAAGCTCAAGTCAATGAACTAATAATGGCAGCAGTGGACAATCCCTCAAATGCTGCTGAATGGGCAATTGCAGAGATGATAAATCAACCTGAACTCTTTGAGAAAGCAACACAAGAACTTGATGCTGTGATCGGAAAGAACAGACAAGTTCAAGAGTCGGATTTGTCAAAGCTCAACTTCGTAAAGTCCTGCGCCAGAGAAGCCTTCCGCCTCCACCCGGTGGCACCGTTCAATGTGCCTCACGTGTCTATGGCCGACACAACCGTCGGAGATTACTTCATCCCCAAGGGTAGCCACGTGATTCTCAGCCGAGTCGGACTCGGCCGCAACCCTAAAGTTTGGGACGAGCCTCTCAAGTTCAAGCCGGAGCGGCACCTCAAGGACGACGGGTCAGGGGTTGTGCTCACCGAGTCGGAGTTGCGGTTCATTTCGTTCAGCACGGGGATGCGGGGCTGCGTAGCGAGTACACTCGGCACTGCCATGACCGTAATGCTGTTTGCTAGGCTTCTTCATGGGTTTACATGGCACGTGCCACCCACTGAGTCAAGCATCGAACTCACCGAGTCAACTAACGATCTCTTACTCGCGAAGCCACTGCTTGCATATGCAAAGCCACGTTTGCCTGCTCATGTTTACCAAACGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00966 ko01110 ko01210 map00460 map00966 map01110 map01210
Pfam Domains
Protein Families
p450

Protein Analysis

535

Amino Acids

60.92

Weight (kDa)

7.94

Isoelectric Point (pI)

35.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 47 - 516 1.4e-82 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1220
AccB1I GGYRCC 3 cut(s) 403, 1180, 1329
AccB7I CCANNNNNTGG 1 cut(s) 1177
AccBSI CCGCTC 1 cut(s) 1327
AccII CGCG 3 cut(s) 309, 346, 1544
AciI CCGC 8 cut(s) 307, 395, 555, 1165, 1282, 1327, 1378, 1405
AclI AACGTT 2 cut(s) 12, 67
AcoI YGGCCR 3 cut(s) 775, 1209, 1279
AcsI RAATTY 1 cut(s) 568
AcvI CACGTG 3 cut(s) 1201, 1255, 1486
AdeI CACNNNGTG 1 cut(s) 1177
AfaI GTAC 4 cut(s) 386, 530, 635, 1423
AfiI CCNNNNNNNGG 4 cut(s) 167, 207, 1174, 1177
AflIII ACRYGT 1 cut(s) 1200
AgsI TTSAA 5 cut(s) 112, 500, 1109, 1189, 1318
AhdI GACNNNNNGTC 1 cut(s) 1345
AjuI GAANNNNNNNTTGG 1 cut(s) 29
AleI CACNNNNGTG 1 cut(s) 275
AloI GAACNNNNNNTCC 2 cut(s) 1364, 1396
AluBI AGCT 2 cut(s) 962, 1129
AluI AGCT 2 cut(s) 962, 1129
Alw21I GWGCWC 1 cut(s) 1362
Alw26I GTCTC 1 cut(s) 948
AoxI GGCC 6 cut(s) 158, 504, 662, 775, 1209, 1279
ApeKI GCWGC 3 cut(s) 986, 1010, 1410
ApoI RAATTY 1 cut(s) 568
ArsI GACNNNNNNTTYG 4 cut(s) 320, 352, 951, 983
AspLEI GCGC 1 cut(s) 1151
AspS9I GGNCC 1 cut(s) 662
AsuC2I CCSGG 1 cut(s) 1175
AsuHPI GGTGA 4 cut(s) 34, 367, 1354, 1507
BaeGI GKGCMC 1 cut(s) 408
BanI GGYRCC 3 cut(s) 403, 1180, 1329
BauI CACGAG 1 cut(s) 218
BbrPI CACGTG 3 cut(s) 1201, 1255, 1486
Bbv12I GWGCWC 1 cut(s) 1362
BbvI GCAGC 3 cut(s) 997, 998, 1397
BccI CCATC 3 cut(s) 220, 293, 650
BceAI ACGGC 1 cut(s) 790
BcgI CGANNNNNNTGC 4 cut(s) 436, 470, 1534, 1568
BciVI GTATCC 1 cut(s) 207
BclI TGATCA 1 cut(s) 60
BcnI CCSGG 1 cut(s) 1175
BcoDI GTCTC 1 cut(s) 948
BfaI CTAG 2 cut(s) 38, 1458
BfuI GTATCC 1 cut(s) 207
BglI GCCNNNNNGGC 1 cut(s) 1327
BisI GCNGC 6 cut(s) 307, 987, 1011, 1282, 1328, 1411
BlsI GCNGC 6 cut(s) 308, 988, 1012, 1283, 1329, 1412
Bme1390I CCNGG 1 cut(s) 1175
BmeRI GACNNNNNGTC 1 cut(s) 1345
BmgT120I GGNCC 1 cut(s) 662
BmiI GGNNCC 3 cut(s) 405, 1182, 1331
BmrFI CCNGG 1 cut(s) 1175
BmsI GCATC 8 cut(s) 85, 264, 350, 743, 787, 1069, 1392, 1515
BplI GAGNNNNNCTC 2 cut(s) 1260, 1292
BpuEI CTTGAG 4 cut(s) 775, 948, 1295, 1319
BpuMI CCSGG 1 cut(s) 1175
BsaAI YACGTR 3 cut(s) 1201, 1255, 1486
BsaBI GATNNNNATC 1 cut(s) 284
BsaJI CCNNGG 3 cut(s) 155, 378, 1242
BsaWI WCCGGW 1 cut(s) 207
BsaXI ACNNNNNCTCC 2 cut(s) 1364, 1394
Bsc4I CCNNNNNNNGG 4 cut(s) 167, 207, 1174, 1177
Bse1I ACTGG 1 cut(s) 535
Bse8I GATNNNNATC 1 cut(s) 284
BseDI CCNNGG 3 cut(s) 155, 378, 1242
BseGI GGATG 6 cut(s) 255, 661, 734, 802, 1236, 1407
BseJI GATNNNNATC 1 cut(s) 284
BseLI CCNNNNNNNGG 4 cut(s) 167, 207, 1174, 1177
BseMII CTCAG 3 cut(s) 316, 1276, 1488
BseNI ACTGG 1 cut(s) 535
BseSI GKGCMC 1 cut(s) 408
BseX3I CGGCCG 1 cut(s) 1279
BseXI GCAGC 3 cut(s) 997, 998, 1397
Bsh1236I CGCG 3 cut(s) 309, 346, 1544
Bsh1285I CGRYCG 1 cut(s) 1282
BshFI GGCC 6 cut(s) 160, 506, 664, 777, 1211, 1281
BshNI GGYRCC 3 cut(s) 403, 1180, 1329
BsiEI CGRYCG 1 cut(s) 1282
BsiHKAI GWGCWC 1 cut(s) 1362
BsiSI CCGG 3 cut(s) 208, 1175, 1322
BslFI GGGAC 1 cut(s) 1313
BslI CCNNNNNNNGG 4 cut(s) 167, 207, 1174, 1177
BsmAI GTCTC 1 cut(s) 948
BsmFI GGGAC 1 cut(s) 1313
BsnI GGCC 6 cut(s) 160, 506, 664, 777, 1211, 1281
Bsp1286I GDGCHC 2 cut(s) 408, 1362
Bsp1407I TGTACA 1 cut(s) 528
Bsp143I GATC 4 cut(s) 60, 508, 1086, 1531
Bsp19I CCATGG 1 cut(s) 155
Bsp68I TCGCGA 1 cut(s) 1544
BspACI CCGC 8 cut(s) 307, 395, 555, 1165, 1282, 1327, 1378, 1405
BspANI GGCC 6 cut(s) 160, 506, 664, 777, 1211, 1281
BspCNI CTCAG 3 cut(s) 315, 1275, 1489
BspFNI CGCG 3 cut(s) 309, 346, 1544
BspLI GGNNCC 3 cut(s) 405, 1182, 1331
BspT107I GGYRCC 3 cut(s) 403, 1180, 1329
BsrBI CCGCTC 1 cut(s) 1327
BsrGI TGTACA 1 cut(s) 528
BsrI ACTGG 1 cut(s) 535
BssECI CCNNGG 3 cut(s) 155, 378, 1242
BssMI GATC 4 cut(s) 60, 508, 1086, 1531
BssSI CACGAG 1 cut(s) 218
BssT1I CCWWGG 3 cut(s) 155, 378, 1242
Bst2BI CACGAG 1 cut(s) 218
Bst4CI ACNGT 4 cut(s) 942, 1185, 1222, 1444
Bst6I CTCTTC 2 cut(s) 495, 834
BstAUI TGTACA 1 cut(s) 528
BstBAI YACGTR 3 cut(s) 1201, 1255, 1486
BstC8I GCNNGC 3 cut(s) 189, 1557, 1581
BstDEI CTNAG 4 cut(s) 302, 353, 1262, 1497
BstDSI CCRYGG 1 cut(s) 155
BstF5I GGATG 6 cut(s) 255, 661, 734, 802, 1236, 1407
BstFNI CGCG 3 cut(s) 309, 346, 1544
BstHHI GCGC 1 cut(s) 1151
BstKTI GATC 4 cut(s) 63, 511, 1089, 1534
BstMAI GTCTC 1 cut(s) 948
BstMBI GATC 4 cut(s) 60, 508, 1086, 1531
BstMCI CGRYCG 1 cut(s) 1282
BstMWI GCNNNNNNNGC 6 cut(s) 338, 478, 1019, 1157, 1327, 1576
BstNSI RCATGY 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 1173
BstSLI GKGCMC 1 cut(s) 408
BstUI CGCG 3 cut(s) 309, 346, 1544
BstV1I GCAGC 3 cut(s) 997, 998, 1397
BstZI CGGCCG 1 cut(s) 1279
BsuI GTATCC 1 cut(s) 207
BsuRI GGCC 6 cut(s) 160, 506, 664, 777, 1211, 1281
BtgI CCRYGG 1 cut(s) 155
BtgZI GCGATG 1 cut(s) 327
BtsCI GGATG 6 cut(s) 255, 661, 734, 802, 1236, 1407
BtsI GCAGTG 3 cut(s) 996, 1431, 1550
BtsIMutI CAGTG 5 cut(s) 542, 996, 1431, 1494, 1550
BtuMI TCGCGA 1 cut(s) 1544
Cac8I GCNNGC 3 cut(s) 189, 1557, 1581
CfoI GCGC 1 cut(s) 1151
Cfr13I GGNCC 1 cut(s) 662
CseI GACGC 2 cut(s) 335, 338
Csp6I GTAC 4 cut(s) 385, 529, 634, 1422
CviAII CATG 8 cut(s) 156, 252, 750, 779, 1438, 1469, 1479, 1586
CviQI GTAC 4 cut(s) 385, 529, 634, 1422
DdeI CTNAG 4 cut(s) 302, 353, 1262, 1497
DpnI GATC 4 cut(s) 62, 510, 1088, 1533
DpnII GATC 4 cut(s) 60, 508, 1086, 1531
DraIII CACNNNGTG 1 cut(s) 1177
DrdI GACNNNNNNGTC 1 cut(s) 1220
DriI GACNNNNNGTC 1 cut(s) 1345
DseDI GACNNNNNNGTC 1 cut(s) 1220
EaeI YGGCCR 3 cut(s) 775, 1209, 1279
EagI CGGCCG 1 cut(s) 1279
Eam1104I CTCTTC 2 cut(s) 495, 834
Eam1105I GACNNNNNGTC 1 cut(s) 1345
EarI CTCTTC 2 cut(s) 495, 834
EciI GGCGGA 1 cut(s) 1154
EclXI CGGCCG 1 cut(s) 1279
Eco130I CCWWGG 3 cut(s) 155, 378, 1242
Eco52I CGGCCG 1 cut(s) 1279
Eco72I CACGTG 3 cut(s) 1201, 1255, 1486
EcoT14I CCWWGG 3 cut(s) 155, 378, 1242
EcoT22I ATGCAT 1 cut(s) 257
ErhI CCWWGG 3 cut(s) 155, 378, 1242
FaeI CATG 8 cut(s) 159, 255, 753, 782, 1441, 1472, 1482, 1589
FalI AAGNNNNNCTT 4 cut(s) 620, 652, 1118, 1150
FaqI GGGAC 1 cut(s) 1313
FatI CATG 8 cut(s) 155, 251, 749, 778, 1437, 1468, 1478, 1585
FauI CCCGC 1 cut(s) 1398
FauNDI CATATG 1 cut(s) 1561
FbaI TGATCA 1 cut(s) 60
Fnu4HI GCNGC 6 cut(s) 307, 987, 1011, 1282, 1328, 1411
FokI GGATG 6 cut(s) 242, 668, 721, 809, 1223, 1414
Fsp4HI GCNGC 6 cut(s) 307, 987, 1011, 1282, 1328, 1411
FspBI CTAG 2 cut(s) 38, 1458
GlaI GCGC 1 cut(s) 1150
GluI GCNGC 6 cut(s) 307, 987, 1011, 1282, 1328, 1411
HaeIII GGCC 6 cut(s) 160, 506, 664, 777, 1211, 1281
HapII CCGG 3 cut(s) 208, 1175, 1322
HgaI GACGC 2 cut(s) 335, 338
HhaI GCGC 1 cut(s) 1151
Hin1II CATG 8 cut(s) 159, 255, 753, 782, 1441, 1472, 1482, 1589
Hin6I GCGC 1 cut(s) 1149
HinP1I GCGC 1 cut(s) 1149
HincII GTYRAC 1 cut(s) 1524
HindII GTYRAC 1 cut(s) 1524
HinfI GANTC 8 cut(s) 280, 1112, 1258, 1269, 1275, 1367, 1499, 1520
HpaII CCGG 3 cut(s) 208, 1175, 1322
HphI GGTGA 4 cut(s) 34, 367, 1354, 1507
Hpy166II GTNNAC 8 cut(s) 350, 529, 854, 994, 1424, 1476, 1524, 1591
Hpy188III TCNNGA 4 cut(s) 851, 947, 1109, 1543
Hpy8I GTNNAC 8 cut(s) 350, 529, 854, 994, 1424, 1476, 1524, 1591
Hpy99I CGWCG 2 cut(s) 1226, 1346
HpyAV CCTTC 5 cut(s) 55, 418, 430, 545, 1171
HpyCH4III ACNGT 4 cut(s) 942, 1185, 1222, 1444
HpyCH4IV ACGT 6 cut(s) 12, 67, 1200, 1254, 1485, 1573
HpyCH4V TGCA 7 cut(s) 255, 540, 599, 734, 1028, 1559, 1565
HpyF10VI GCNNNNNNNGC 6 cut(s) 338, 478, 1019, 1157, 1327, 1576
HpyF3I CTNAG 4 cut(s) 302, 353, 1262, 1497
HpySE526I ACGT 6 cut(s) 12, 67, 1200, 1254, 1485, 1573
Hsp92II CATG 8 cut(s) 159, 255, 753, 782, 1441, 1472, 1482, 1589
HspAI GCGC 1 cut(s) 1149
Ksp22I TGATCA 1 cut(s) 60
Kzo9I GATC 4 cut(s) 60, 508, 1086, 1531
LmnI GCTCC 1 cut(s) 1324
Lsp1109I GCAGC 3 cut(s) 997, 998, 1397
LweI GCATC 8 cut(s) 85, 264, 350, 743, 787, 1069, 1392, 1515
MaeI CTAG 2 cut(s) 38, 1458
MaeII ACGT 6 cut(s) 12, 67, 1200, 1254, 1485, 1573
MaeIII GTNAC 1 cut(s) 40
MalI GATC 4 cut(s) 62, 510, 1088, 1533
MbiI CCGCTC 1 cut(s) 1327
MboI GATC 4 cut(s) 60, 508, 1086, 1531
MboII GAAGA 7 cut(s) 328, 436, 512, 796, 851, 962, 1457
MfeI CAATTG 1 cut(s) 1023
MhlI GDGCHC 2 cut(s) 408, 1362
MluCI AATT 5 cut(s) 182, 246, 452, 568, 1023
MlyI GAGTC 6 cut(s) 1121, 1269, 1278, 1376, 1508, 1529
MmeI TCCRAC 4 cut(s) 1095, 1204, 1252, 1350
Mph1103I ATGCAT 1 cut(s) 257
MseI TTAA 4 cut(s) 317, 609, 711, 833
MslI CAYNNNNRTG 5 cut(s) 208, 275, 594, 1400, 1436
MspI CCGG 3 cut(s) 208, 1175, 1322
MspR9I CCNGG 1 cut(s) 1175
MunI CAATTG 1 cut(s) 1023
MvnI CGCG 3 cut(s) 309, 346, 1544
MwoI GCNNNNNNNGC 6 cut(s) 338, 478, 1019, 1157, 1327, 1576
NciI CCSGG 1 cut(s) 1175
NcoI CCATGG 1 cut(s) 155
NdeI CATATG 1 cut(s) 1561
NdeII GATC 4 cut(s) 60, 508, 1086, 1531
NlaIII CATG 8 cut(s) 159, 255, 753, 782, 1441, 1472, 1482, 1589
NlaIV GGNNCC 3 cut(s) 405, 1182, 1331
NmeAIII GCCGAG 3 cut(s) 1257, 1292, 1407
NmuCI GTSAC 1 cut(s) 40
NruI TCGCGA 1 cut(s) 1544
NsiI ATGCAT 1 cut(s) 257
NspI RCATGY 1 cut(s) 255
OliI CACNNNNGTG 1 cut(s) 275
PcsI WCGNNNNNNNCGW 1 cut(s) 1515
PfeI GAWTC 2 cut(s) 280, 1258
PflMI CCANNNNNTGG 1 cut(s) 1177
PkrI GCNGC 6 cut(s) 308, 988, 1012, 1283, 1329, 1412
PleI GAGTC 6 cut(s) 1120, 1269, 1277, 1375, 1507, 1528
PmaCI CACGTG 3 cut(s) 1201, 1255, 1486
PmlI CACGTG 3 cut(s) 1201, 1255, 1486
PpsI GAGTC 6 cut(s) 1120, 1269, 1277, 1375, 1507, 1528
Ppu21I YACGTR 3 cut(s) 1201, 1255, 1486
Psp1406I AACGTT 2 cut(s) 12, 67
PspCI CACGTG 3 cut(s) 1201, 1255, 1486
PspN4I GGNNCC 3 cut(s) 405, 1182, 1331
PspPI GGNCC 1 cut(s) 662
RruI TCGCGA 1 cut(s) 1544
RsaI GTAC 4 cut(s) 386, 530, 635, 1423
RsaNI GTAC 4 cut(s) 385, 529, 634, 1422
RseI CAYNNNNRTG 5 cut(s) 208, 275, 594, 1400, 1436
SaqAI TTAA 4 cut(s) 317, 609, 711, 833
SatI GCNGC 6 cut(s) 307, 987, 1011, 1282, 1328, 1411
Sau3AI GATC 4 cut(s) 60, 508, 1086, 1531
Sau96I GGNCC 1 cut(s) 662
SchI GAGTC 6 cut(s) 1121, 1269, 1278, 1376, 1508, 1529
ScrFI CCNGG 1 cut(s) 1175
SduI GDGCHC 2 cut(s) 408, 1362
SfaNI GCATC 8 cut(s) 85, 264, 350, 743, 787, 1069, 1392, 1515
SmiMI CAYNNNNRTG 5 cut(s) 208, 275, 594, 1400, 1436
SmlI CTYRAG 4 cut(s) 754, 963, 1310, 1334
SmoI CTYRAG 4 cut(s) 754, 963, 1310, 1334
Sse9I AATT 5 cut(s) 182, 246, 452, 568, 1023
SsiI CCGC 8 cut(s) 307, 395, 555, 1165, 1282, 1327, 1378, 1405
SspMI CTAG 2 cut(s) 38, 1458
StyD4I CCNGG 1 cut(s) 1173
StyI CCWWGG 3 cut(s) 155, 378, 1242
TaaI ACNGT 4 cut(s) 942, 1185, 1222, 1444
TaiI ACGT 6 cut(s) 15, 70, 1203, 1257, 1488, 1576
TaqI TCGA 2 cut(s) 837, 1509
TasI AATT 5 cut(s) 182, 246, 452, 568, 1023
TatI WGTACW 2 cut(s) 528, 1421
TauI GCSGC 3 cut(s) 309, 1284, 1330
TfiI GAWTC 2 cut(s) 280, 1258
Tru1I TTAA 4 cut(s) 317, 609, 711, 833
Tru9I TTAA 4 cut(s) 317, 609, 711, 833
TscAI CASTG 5 cut(s) 542, 996, 1438, 1501, 1557
TseFI GTSAC 1 cut(s) 40
TseI GCWGC 3 cut(s) 986, 1010, 1410
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 8 cut(s) 37, 251, 564, 714, 987, 1225, 1372, 1457
TspGWI ACGGA 2 cut(s) 248, 500
TspRI CASTG 5 cut(s) 542, 996, 1438, 1501, 1557
Van91I CCANNNNNTGG 1 cut(s) 1177
XapI RAATTY 1 cut(s) 568
XceI RCATGY 1 cut(s) 255
XspI CTAG 2 cut(s) 38, 1458
Zsp2I ATGCAT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.