MD11G1059800.v1.1

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0017490
Physical Location & Seq
Forward (+)
5200245 .. 5201771
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1059800.v1.1.491

Sequence Viewer

Length: 1272 bp
ATGATGGACGAAATGAACACCGAAATTGCATGCATCCGTCTCGGAAACACGAATGTGATTCCCATCACATGCCCTCAACTCAGCCGCGAAATCTTAAAGAAGCAGGATGCAATTTTTGCCTCGCGGCCGCTTAGCATTTCCACTTTCCTCATTACCAAAGGGTACATAACTACAGTTATGGTGCCCTTCGGTGAACAATGGAAGAAAATGGGGAAGGTGATCACGAGCGAATTGCTTTCTCCGATGAGGCACAAGTGGCTCACCGACAAAAGGGTCGAGGAGGCCAATCACATAGTTAAGTATGTGTACAACCAGTGTGAAGATAATGAAGGTGGTGGCATTGTAAATTTGAGACTTGCCACTCAACATTATTGTGCAAATGTGATTAAGAAAATGGTTCTCAACAAGAGGTACTTTGGGGATGAAATGGAGGATGGTGGGCCTGGTTTGGAGGAGCAAAAGTATTTAGAAAATGTATTTACAATGCTTAACTACATCTATTCATTTTCTGCATCCGATTACATTCCATGCTTGAGAGGGCTTGACTTGGATGGCCATGAGAAAATTATCAAGGATGCCATAAGAGATACAAGAAAATACCAAGACCCCTTAATCGAAGAGAGGGTTCATAAGAATGGCGAACCCTTACTTTCTCTGGAAGAAATCAAAGCCCAAGTCAATGAACTAATAATGGCAGCAGTGGACAATCCCTCGAATGCTGCTGAATGGGCAATTGCAGAGATGATAAATCAGCCCGAACTCTTTCAGAAAGCAACACAAGAGCTTGACGCTATAGTCGGAAAGAACAGACAAGTTCAAGAAAGCCTTCCGCCTCCACCGGTGGCACCGTTCAATGTGCCCCATGTGTCCATGGCCGACACCACCGTCGGAGATTACTTCATCCCCAAGGGTAGCCACGTGATTCTCAGCCGAGTCGGACTCGGCCGCAACCCTAAAGTTTGGGACGAGCCTCTTAAGTTCAAGCCGGAGCGGCACCTCAAGGACGATGGGTCAGGGGTGGTGCTCACCGAGTCGGAGTTGCGGTTCATTTCGTTCAGCACGGGAATGCGGGGCTGCGTAGCCAGTGGACTGGGCACTGCCATGACCGTCATGCTGTTTGCTAGGCTTCTTCCTGGGTTTACATGGCACGTGCCACCTACTGAGTCAAGCATTGAGCTCACCGAATCTCATAACGACCTCTTACTTGCTAAGCCACTTCTTGCGTACGCGAAGCCGCGTTTGGCAGCTCACGTGTACAATACGGGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00966 ko01110 ko01210 map00460 map00966 map01110 map01210
Pfam Domains
Protein Families
p450

Protein Analysis

424

Amino Acids

47.5

Weight (kDa)

5.94

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 8 - 210 5.6e-11 Cytochrome P450
p450 PF00067 211 - 404 8.3e-39 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 272, 794, 884
AccB1I GGYRCC 3 cut(s) 181, 844, 993
AccBSI CCGCTC 1 cut(s) 991
AccII CGCG 4 cut(s) 87, 124, 1229, 1237
AciI CCGC 9 cut(s) 85, 124, 128, 830, 946, 991, 1042, 1069, 1235
AcoI YGGCCR 4 cut(s) 125, 553, 873, 943
AcsI RAATTY 1 cut(s) 346
AcvI CACGTG 3 cut(s) 919, 1150, 1252
AfaI GTAC 5 cut(s) 164, 308, 413, 1226, 1256
AfiI CCNNNNNNNGG 1 cut(s) 270
AflII CTTAAG 1 cut(s) 974
AflIII ACRYGT 1 cut(s) 1251
AgeI ACCGGT 1 cut(s) 838
AgsI TTSAA 3 cut(s) 818, 853, 982
AhdI GACNNNNNGTC 1 cut(s) 1009
AjnI CCWGG 2 cut(s) 442, 1132
AjuI GAANNNNNNNTTGG 2 cut(s) 1223, 1255
AleI CACNNNNGTG 1 cut(s) 53
AloI GAACNNNNNNTCC 2 cut(s) 1028, 1060
AluBI AGCT 3 cut(s) 784, 1177, 1247
AluI AGCT 3 cut(s) 784, 1177, 1247
Alw21I GWGCWC 2 cut(s) 1026, 1179
Alw26I GTCTC 2 cut(s) 44, 346
AoxI GGCC 6 cut(s) 125, 282, 440, 553, 873, 943
ApeKI GCWGC 4 cut(s) 695, 719, 1074, 1244
ApoI RAATTY 1 cut(s) 346
ArsI GACNNNNNNTTYG 2 cut(s) 660, 692
AsiGI ACCGGT 1 cut(s) 838
Asp700I GAANNNNTTC 2 cut(s) 762, 825
AspS9I GGNCC 1 cut(s) 440
AsuHPI GGTGA 5 cut(s) 203, 229, 253, 1018, 1171
BaeGI GKGCMC 3 cut(s) 186, 861, 1097
BalI TGGCCA 1 cut(s) 555
BanI GGYRCC 3 cut(s) 181, 844, 993
BanII GRGCYC 1 cut(s) 1179
BauI CACGAG 1 cut(s) 223
BbrPI CACGTG 3 cut(s) 919, 1150, 1252
Bbv12I GWGCWC 2 cut(s) 1026, 1179
BbvI GCAGC 4 cut(s) 706, 707, 1061, 1256
BccI CCATC 4 cut(s) 71, 428, 545, 1001
BcgI CGANNNNNNTGC 4 cut(s) 22, 56, 214, 248
BciT130I CCWGG 2 cut(s) 444, 1134
BclI TGATCA 1 cut(s) 219
BcoDI GTCTC 2 cut(s) 44, 346
BfaI CTAG 1 cut(s) 1122
BfmI CTRYAG 2 cut(s) 171, 792
BfrI CTTAAG 1 cut(s) 974
BglI GCCNNNNNGGC 1 cut(s) 991
BlpI GCTNAGC 2 cut(s) 131, 1209
Bme1390I CCNGG 2 cut(s) 444, 1134
BmeRI GACNNNNNGTC 1 cut(s) 1009
BmgT120I GGNCC 1 cut(s) 440
BmiI GGNNCC 3 cut(s) 183, 846, 995
BmrFI CCNGG 2 cut(s) 444, 1134
BmrI ACTGGG 1 cut(s) 1100
BmsI GCATC 4 cut(s) 42, 97, 521, 565
BmuI ACTGGG 1 cut(s) 1100
BplI GAGNNNNNCTC 2 cut(s) 924, 956
Bpu1102I GCTNAGC 2 cut(s) 131, 1209
BpuEI CTTGAG 2 cut(s) 553, 983
BsaAI YACGTR 3 cut(s) 919, 1150, 1252
BsaBI GATNNNNATC 1 cut(s) 62
BsaJI CCNNGG 3 cut(s) 870, 906, 1133
BsaWI WCCGGW 1 cut(s) 838
BsaXI ACNNNNNCTCC 4 cut(s) 446, 476, 1028, 1058
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse118I RCCGGY 1 cut(s) 838
Bse1I ACTGG 3 cut(s) 313, 1083, 1095
Bse8I GATNNNNATC 1 cut(s) 62
BseBI CCWGG 2 cut(s) 444, 1134
BseDI CCNNGG 3 cut(s) 870, 906, 1133
BseGI GGATG 8 cut(s) 33, 112, 427, 439, 512, 556, 580, 900
BseJI GATNNNNATC 1 cut(s) 62
BseLI CCNNNNNNNGG 1 cut(s) 270
BseMII CTCAG 3 cut(s) 94, 940, 1152
BseNI ACTGG 3 cut(s) 313, 1083, 1095
BseRI GAGGAG 2 cut(s) 293, 467
BseSI GKGCMC 3 cut(s) 186, 861, 1097
BseX3I CGGCCG 2 cut(s) 125, 943
BseXI GCAGC 4 cut(s) 706, 707, 1061, 1256
Bsh1236I CGCG 4 cut(s) 87, 124, 1229, 1237
Bsh1285I CGRYCG 2 cut(s) 128, 946
BshFI GGCC 6 cut(s) 127, 284, 442, 555, 875, 945
BshNI GGYRCC 3 cut(s) 181, 844, 993
BshTI ACCGGT 1 cut(s) 838
BsiEI CGRYCG 2 cut(s) 128, 946
BsiHKAI GWGCWC 2 cut(s) 1026, 1179
BsiSI CCGG 2 cut(s) 839, 986
BsiWI CGTACG 1 cut(s) 1224
BslFI GGGAC 1 cut(s) 977
BslI CCNNNNNNNGG 1 cut(s) 270
BsmAI GTCTC 2 cut(s) 44, 346
BsmBI CGTCTC 1 cut(s) 44
BsmFI GGGAC 1 cut(s) 977
BsmI GAATGC 2 cut(s) 721, 1071
BsnI GGCC 6 cut(s) 127, 284, 442, 555, 875, 945
Bsp1286I GDGCHC 5 cut(s) 186, 861, 1026, 1097, 1179
Bsp1407I TGTACA 2 cut(s) 306, 1254
Bsp143I GATC 1 cut(s) 219
Bsp1720I GCTNAGC 2 cut(s) 131, 1209
Bsp19I CCATGG 1 cut(s) 870
BspACI CCGC 9 cut(s) 85, 124, 128, 830, 946, 991, 1042, 1069, 1235
BspANI GGCC 6 cut(s) 127, 284, 442, 555, 875, 945
BspCNI CTCAG 3 cut(s) 93, 939, 1153
BspFNI CGCG 4 cut(s) 87, 124, 1229, 1237
BspLI GGNNCC 3 cut(s) 183, 846, 995
BspT107I GGYRCC 3 cut(s) 181, 844, 993
BspTI CTTAAG 1 cut(s) 974
BsrBI CCGCTC 1 cut(s) 991
BsrFI RCCGGY 1 cut(s) 838
BsrGI TGTACA 2 cut(s) 306, 1254
BsrI ACTGG 3 cut(s) 313, 1083, 1095
BssAI RCCGGY 1 cut(s) 838
BssECI CCNNGG 3 cut(s) 870, 906, 1133
BssMI GATC 1 cut(s) 219
BssSI CACGAG 1 cut(s) 223
BssT1I CCWWGG 2 cut(s) 870, 906
Bst2BI CACGAG 1 cut(s) 223
Bst2UI CCWGG 2 cut(s) 444, 1134
Bst4CI ACNGT 4 cut(s) 175, 849, 886, 1108
Bst6I CTCTTC 1 cut(s) 612
BstAFI CTTAAG 1 cut(s) 974
BstAPI GCANNNNNTGC 1 cut(s) 116
BstAUI TGTACA 2 cut(s) 306, 1254
BstBAI YACGTR 3 cut(s) 919, 1150, 1252
BstC8I GCNNGC 1 cut(s) 31
BstDEI CTNAG 5 cut(s) 80, 131, 926, 1161, 1209
BstDSI CCRYGG 1 cut(s) 870
BstF5I GGATG 8 cut(s) 33, 112, 427, 439, 512, 556, 580, 900
BstFNI CGCG 4 cut(s) 87, 124, 1229, 1237
BstKTI GATC 1 cut(s) 222
BstMAI GTCTC 2 cut(s) 44, 346
BstMBI GATC 1 cut(s) 219
BstMCI CGRYCG 2 cut(s) 128, 946
BstMWI GCNNNNNNNGC 4 cut(s) 116, 256, 728, 991
BstNI CCWGG 2 cut(s) 444, 1134
BstNSI RCATGY 2 cut(s) 33, 72
BstSCI CCNGG 2 cut(s) 442, 1132
BstSFI CTRYAG 2 cut(s) 171, 792
BstSLI GKGCMC 3 cut(s) 186, 861, 1097
BstUI CGCG 4 cut(s) 87, 124, 1229, 1237
BstV1I GCAGC 4 cut(s) 706, 707, 1061, 1256
BstXI CCANNNNNNTGG 1 cut(s) 1090
BstZI CGGCCG 2 cut(s) 125, 943
BsuRI GGCC 6 cut(s) 127, 284, 442, 555, 875, 945
BtgI CCRYGG 1 cut(s) 870
BtsCI GGATG 8 cut(s) 33, 112, 427, 439, 512, 556, 580, 900
BtsI GCAGTG 2 cut(s) 705, 1095
BtsIMutI CAGTG 4 cut(s) 320, 705, 1090, 1095
Cac8I GCNNGC 1 cut(s) 31
CciNI GCGGCCGC 1 cut(s) 125
Cfr10I RCCGGY 1 cut(s) 838
Cfr13I GGNCC 1 cut(s) 440
CseI GACGC 1 cut(s) 797
Csp6I GTAC 5 cut(s) 163, 307, 412, 1225, 1255
CspAI ACCGGT 1 cut(s) 838
CviAII CATG 9 cut(s) 30, 69, 528, 557, 863, 871, 1102, 1111, 1143
CviQI GTAC 5 cut(s) 163, 307, 412, 1225, 1255
DdeI CTNAG 5 cut(s) 80, 131, 926, 1161, 1209
DpnI GATC 1 cut(s) 221
DpnII GATC 1 cut(s) 219
DrdI GACNNNNNNGTC 3 cut(s) 272, 794, 884
DriI GACNNNNNGTC 1 cut(s) 1009
DseDI GACNNNNNNGTC 3 cut(s) 272, 794, 884
EaeI YGGCCR 4 cut(s) 125, 553, 873, 943
EagI CGGCCG 2 cut(s) 125, 943
Eam1104I CTCTTC 1 cut(s) 612
Eam1105I GACNNNNNGTC 1 cut(s) 1009
EarI CTCTTC 1 cut(s) 612
EciI GGCGGA 1 cut(s) 819
Ecl136II GAGCTC 1 cut(s) 1177
EclXI CGGCCG 2 cut(s) 125, 943
Eco130I CCWWGG 2 cut(s) 870, 906
Eco24I GRGCYC 1 cut(s) 1179
Eco52I CGGCCG 2 cut(s) 125, 943
Eco53kI GAGCTC 1 cut(s) 1177
Eco72I CACGTG 3 cut(s) 919, 1150, 1252
EcoICRI GAGCTC 1 cut(s) 1177
EcoRII CCWGG 2 cut(s) 442, 1132
EcoT14I CCWWGG 2 cut(s) 870, 906
EcoT22I ATGCAT 1 cut(s) 35
EcoT38I GRGCYC 1 cut(s) 1179
ErhI CCWWGG 2 cut(s) 870, 906
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 9 cut(s) 33, 72, 531, 560, 866, 874, 1105, 1114, 1146
FalI AAGNNNNNCTT 4 cut(s) 398, 430, 810, 842
FaqI GGGAC 1 cut(s) 977
FatI CATG 9 cut(s) 29, 68, 527, 556, 862, 870, 1101, 1110, 1142
FauI CCCGC 1 cut(s) 1062
FbaI TGATCA 1 cut(s) 219
FokI GGATG 8 cut(s) 20, 119, 434, 446, 499, 563, 587, 887
FriOI GRGCYC 1 cut(s) 1179
FspBI CTAG 1 cut(s) 1122
HaeIII GGCC 6 cut(s) 127, 284, 442, 555, 875, 945
HapII CCGG 2 cut(s) 839, 986
HgaI GACGC 1 cut(s) 797
Hin1II CATG 9 cut(s) 33, 72, 531, 560, 866, 874, 1105, 1114, 1146
HinfI GANTC 7 cut(s) 58, 922, 933, 939, 1031, 1163, 1184
HpaII CCGG 2 cut(s) 839, 986
HphI GGTGA 5 cut(s) 203, 229, 253, 1018, 1171
Hpy166II GTNNAC 6 cut(s) 194, 307, 703, 1088, 1140, 1255
Hpy188I TCNGA 8 cut(s) 44, 243, 517, 768, 800, 890, 938, 1036
Hpy188III TCNNGA 3 cut(s) 223, 656, 818
Hpy8I GTNNAC 6 cut(s) 194, 307, 703, 1088, 1140, 1255
Hpy99I CGWCG 1 cut(s) 890
HpyAV CCTTC 4 cut(s) 196, 208, 323, 836
HpyCH4III ACNGT 4 cut(s) 175, 849, 886, 1108
HpyCH4IV ACGT 3 cut(s) 918, 1149, 1251
HpyCH4V TGCA 6 cut(s) 29, 33, 110, 377, 512, 737
HpyF10VI GCNNNNNNNGC 4 cut(s) 116, 256, 728, 991
HpyF3I CTNAG 5 cut(s) 80, 131, 926, 1161, 1209
HpySE526I ACGT 3 cut(s) 918, 1149, 1251
Hsp92II CATG 9 cut(s) 33, 72, 531, 560, 866, 874, 1105, 1114, 1146
Ksp22I TGATCA 1 cut(s) 219
Kzo9I GATC 1 cut(s) 219
LmnI GCTCC 2 cut(s) 454, 988
Lsp1109I GCAGC 4 cut(s) 706, 707, 1061, 1256
LweI GCATC 4 cut(s) 42, 97, 521, 565
MaeI CTAG 1 cut(s) 1122
MaeII ACGT 3 cut(s) 918, 1149, 1251
MalI GATC 1 cut(s) 221
MbiI CCGCTC 1 cut(s) 991
MboI GATC 1 cut(s) 219
MboII GAAGA 5 cut(s) 214, 332, 629, 671, 1121
MfeI CAATTG 1 cut(s) 732
MhlI GDGCHC 5 cut(s) 186, 861, 1026, 1097, 1179
MlsI TGGCCA 1 cut(s) 555
MluCI AATT 7 cut(s) 24, 111, 230, 346, 564, 732, 1267
MluNI TGGCCA 1 cut(s) 555
MlyI GAGTC 4 cut(s) 933, 942, 1040, 1172
MmeI TCCRAC 4 cut(s) 778, 868, 916, 1014
Mox20I TGGCCA 1 cut(s) 555
Mph1103I ATGCAT 1 cut(s) 35
MroXI GAANNNNTTC 2 cut(s) 762, 825
MscI TGGCCA 1 cut(s) 555
MseI TTAA 6 cut(s) 95, 297, 387, 489, 611, 975
MslI CAYNNNNRTG 5 cut(s) 53, 372, 633, 1064, 1100
Msp20I TGGCCA 1 cut(s) 555
MspCI CTTAAG 1 cut(s) 974
MspI CCGG 2 cut(s) 839, 986
MspR9I CCNGG 2 cut(s) 444, 1134
MunI CAATTG 1 cut(s) 732
Mva1269I GAATGC 2 cut(s) 721, 1071
MvaI CCWGG 2 cut(s) 444, 1134
MvnI CGCG 4 cut(s) 87, 124, 1229, 1237
MwoI GCNNNNNNNGC 4 cut(s) 116, 256, 728, 991
NcoI CCATGG 1 cut(s) 870
NdeII GATC 1 cut(s) 219
NlaIII CATG 9 cut(s) 33, 72, 531, 560, 866, 874, 1105, 1114, 1146
NlaIV GGNNCC 3 cut(s) 183, 846, 995
NmeAIII GCCGAG 2 cut(s) 921, 956
NotI GCGGCCGC 1 cut(s) 125
NsiI ATGCAT 1 cut(s) 35
NspI RCATGY 2 cut(s) 33, 72
OliI CACNNNNGTG 1 cut(s) 53
PaeI GCATGC 1 cut(s) 33
PctI GAATGC 2 cut(s) 721, 1071
PdmI GAANNNNTTC 2 cut(s) 762, 825
PfeI GAWTC 3 cut(s) 58, 922, 1184
Pfl23II CGTACG 1 cut(s) 1224
PinAI ACCGGT 1 cut(s) 838
PleI GAGTC 4 cut(s) 933, 941, 1039, 1171
PmaCI CACGTG 3 cut(s) 919, 1150, 1252
PmlI CACGTG 3 cut(s) 919, 1150, 1252
PpsI GAGTC 4 cut(s) 933, 941, 1039, 1171
Ppu21I YACGTR 3 cut(s) 919, 1150, 1252
Psp124BI GAGCTC 1 cut(s) 1179
Psp6I CCWGG 2 cut(s) 442, 1132
PspCI CACGTG 3 cut(s) 919, 1150, 1252
PspGI CCWGG 2 cut(s) 442, 1132
PspLI CGTACG 1 cut(s) 1224
PspN4I GGNNCC 3 cut(s) 183, 846, 995
PspPI GGNCC 1 cut(s) 440
RsaI GTAC 5 cut(s) 164, 308, 413, 1226, 1256
RsaNI GTAC 5 cut(s) 163, 307, 412, 1225, 1255
RseI CAYNNNNRTG 5 cut(s) 53, 372, 633, 1064, 1100
SacI GAGCTC 1 cut(s) 1179
SaqAI TTAA 6 cut(s) 95, 297, 387, 489, 611, 975
Sau3AI GATC 1 cut(s) 219
Sau96I GGNCC 1 cut(s) 440
SchI GAGTC 4 cut(s) 933, 942, 1040, 1172
ScrFI CCNGG 2 cut(s) 444, 1134
SduI GDGCHC 5 cut(s) 186, 861, 1026, 1097, 1179
SfaNI GCATC 4 cut(s) 42, 97, 521, 565
SfcI CTRYAG 2 cut(s) 171, 792
SgrAI CRCCGGYG 1 cut(s) 838
SmiMI CAYNNNNRTG 5 cut(s) 53, 372, 633, 1064, 1100
SmlI CTYRAG 3 cut(s) 532, 974, 998
SmoI CTYRAG 3 cut(s) 532, 974, 998
SphI GCATGC 1 cut(s) 33
Sse9I AATT 7 cut(s) 24, 111, 230, 346, 564, 732, 1267
SsiI CCGC 9 cut(s) 85, 124, 128, 830, 946, 991, 1042, 1069, 1235
SspMI CTAG 1 cut(s) 1122
SstI GAGCTC 1 cut(s) 1179
StyD4I CCNGG 2 cut(s) 442, 1132
StyI CCWWGG 2 cut(s) 870, 906
TaaI ACNGT 4 cut(s) 175, 849, 886, 1108
TaiI ACGT 3 cut(s) 921, 1152, 1254
TaqI TCGA 3 cut(s) 276, 615, 713
TasI AATT 7 cut(s) 24, 111, 230, 346, 564, 732, 1267
TatI WGTACW 2 cut(s) 306, 1254
TauI GCSGC 6 cut(s) 87, 127, 130, 948, 994, 1237
TfiI GAWTC 3 cut(s) 58, 922, 1184
Tru1I TTAA 6 cut(s) 95, 297, 387, 489, 611, 975
Tru9I TTAA 6 cut(s) 95, 297, 387, 489, 611, 975
TscAI CASTG 4 cut(s) 320, 705, 1090, 1102
TseI GCWGC 4 cut(s) 695, 719, 1074, 1244
TspDTI ATGAA 8 cut(s) 29, 342, 438, 492, 617, 696, 889, 1036
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 4 cut(s) 320, 705, 1090, 1102
Vha464I CTTAAG 1 cut(s) 974
XapI RAATTY 1 cut(s) 346
XceI RCATGY 2 cut(s) 33, 72
XmnI GAANNNNTTC 2 cut(s) 762, 825
XspI CTAG 1 cut(s) 1122
Zsp2I ATGCAT 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.