MD11G1099600.v1.1

lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0022434
Physical Location & Seq
Forward (+)
8333657 .. 8338385
4729 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1099600.v1.1.491

Sequence Viewer

Length: 1392 bp
ATGTCTGATAATGGAAAGCTTCTCAAAGAGGTATATACTTTGCTTCATCTATCCACTACACGTCTGATTAGGGTTCTATTAAATGTTTTCTCCTTTCGTTCTCCTTATTATAAGGGTCATCATATACCATTGCTCCCAACTACATTAGTTTCTCAGACAACAATGGCAGCATTTAATCGTGGAAAATATACCACAATTCTCAGCATTGACGGAGGAGGAGTGAGAGGCATCATCCCGGCCACCATACTTGACTGTCTTGAATCCGAACTCCAGAAACTGGATGGTGAAGATGCAAGGATTGCAGACTACTTCGACTTCATTGCCGGAACCAGCACGGGAGGCCTTATCACAGCCATGCTCACCAAACCAGATGAAAAAAACAAACCTCTTTTTGCAGCAAATAAAATCGTTGAATTTTTTGTTGAAGAGGCTCCAAAATTCTTCCCTCCACCAAATCCTGTGGAGGAGCCAAGAATGAGAGAAACAGACGGCATATTCTTGAAGAATTTTAGAAAGTTGAAGAGGCTGGCAGGAAACTACTTGCAGGAGGGTTTTCTACGGCTTACGGGACCAAAGTTCGATGGTGTTTATCTGCGAAACAAGATCGTGCAGACCGTTGGAGAAACAAAGATTCGAGATACCTTAACCAACATTATCATTCCCAGTTGTGATATGAAGTACCTCCATCCGGTCGTCTTCTCCACTTTGAAGGCAAAACGAGACCACTCAAAGGATGTTCTGCTAAGAGATGTCTGCATTGCTACATCTGCAGCACCGTTCTATCTCCCTCCTCACAAATTTGAGCAGGGCACAATAAAATATGACCTAGTCGATGGTGGTGTTGCAGCCAACAATCCCACTTTGCTTGCAATCATTGAAAGTGCCAAGGAGAAGTCTGATAATAAAAGTGGGGCAGAGCGCCTGCATATTGATAGTAGTAAGCTTCTGGTTCTGTCCCTGGGAACTGGATCTGCAAAGAAGGGTGCGATGCTGGAAGTCGGAAATCGCGGCGAGTGGGGAATCTTGAAATGGTTGGTGAATCCAAAAAGCAGCAGTATCCCTTTAATTGATGTTCTTACGACTCCAAGTGTCAACATGGTTGAAGTATACCTGTCTGCTTTCTTCAATGTCTCCGGCTCTGATGACAATTATCTCCGGATCCAGGAGGATGGCTTGAAACCTGATGAAATTGATATGACTGATTCAAGTCCTGAAAATCTCAAAAAACTTGTGAAGGCTGGGACTGACCTCCTTGAAAAAACTGTCAGTGCAATGAACCTCGACACTGGCTGGTATGATAAACCAACTGACATGACATGCAAATACAAAGACGCAATTGCAGGATTTGCCCAAAAACTGTCACGTGAAAAAAAGAGGCGAGCAAATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

464

Amino Acids

51.43

Weight (kDa)

8.82

Isoelectric Point (pI)

34.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 67 - 291 1.3e-29 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022434)

Species Orthologous Gene IDs
malus_domestica MD11G1099600.v1.1
pyrus_communis pycom11g08380 pycom11g08390 pycom11g08410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 111
AccB7I CCANNNNNTGG 1 cut(s) 277
AccI GTMKAC 1 cut(s) 1107
AccII CGCG 1 cut(s) 1008
AccIII TCCGGA 1 cut(s) 1155
AciI CCGC 1 cut(s) 1008
AclWI GGATC 3 cut(s) 976, 1153, 1166
AcoI YGGCCR 1 cut(s) 237
AcsI RAATTY 4 cut(s) 413, 437, 505, 797
AcvI CACGTG 1 cut(s) 1364
AfaI GTAC 1 cut(s) 680
AfiI CCNNNNNNNGG 4 cut(s) 277, 688, 730, 1162
AflIII ACRYGT 1 cut(s) 59
AjiI CACGTC 1 cut(s) 62
AjnI CCWGG 2 cut(s) 957, 1161
AluBI AGCT 2 cut(s) 19, 943
AluI AGCT 2 cut(s) 19, 943
Alw26I GTCTC 2 cut(s) 714, 1135
AlwI GGATC 3 cut(s) 976, 1153, 1166
AlwNI CAGNNNCTG 1 cut(s) 277
Aor13HI TCCGGA 1 cut(s) 1155
AoxI GGCC 2 cut(s) 237, 340
ApeKI GCWGC 5 cut(s) 167, 395, 770, 845, 1050
ApoI RAATTY 4 cut(s) 413, 437, 505, 797
AspLEI GCGC 1 cut(s) 921
AspS9I GGNCC 1 cut(s) 569
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 3 cut(s) 296, 352, 1048
AvaII GGWCC 1 cut(s) 569
BaeGI GKGCMC 1 cut(s) 812
BamHI GGATCC 1 cut(s) 1158
BbrPI CACGTG 1 cut(s) 1364
BbsI GAAGAC 1 cut(s) 688
BbvI GCAGC 5 cut(s) 179, 407, 782, 857, 1062
BccI CCATC 5 cut(s) 275, 575, 693, 827, 1163
BceAI ACGGC 2 cut(s) 505, 575
BcgI CGANNNNNNTGC 2 cut(s) 302, 336
BciT130I CCWGG 2 cut(s) 959, 1163
BciVI GTATCC 1 cut(s) 1067
BcnI CCSGG 1 cut(s) 236
BcoDI GTCTC 2 cut(s) 714, 1135
BfaI CTAG 1 cut(s) 827
BfmI CTRYAG 1 cut(s) 768
BfoI RGCGCY 1 cut(s) 922
BfuI GTATCC 1 cut(s) 1067
BisI GCNGC 6 cut(s) 168, 396, 771, 846, 1009, 1051
BlsI GCNGC 6 cut(s) 169, 397, 772, 847, 1010, 1052
Bme1390I CCNGG 3 cut(s) 236, 959, 1163
Bme18I GGWCC 1 cut(s) 569
BmgBI CACGTC 1 cut(s) 62
BmgT120I GGNCC 1 cut(s) 569
BmiI GGNNCC 5 cut(s) 328, 432, 468, 570, 1160
BmrFI CCNGG 3 cut(s) 236, 959, 1163
BmrI ACTGGG 1 cut(s) 657
BmsI GCATC 3 cut(s) 237, 280, 978
BmuI ACTGGG 1 cut(s) 657
BpiI GAAGAC 1 cut(s) 688
BpmI CTGGAG 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 236
BsaAI YACGTR 1 cut(s) 1364
BsaI GGTCTC 1 cut(s) 714
BsaJI CCNNGG 3 cut(s) 885, 957, 958
BsaWI WCCGGW 2 cut(s) 688, 1155
BsaXI ACNNNNNCTCC 4 cut(s) 117, 147, 204, 234
Bsc4I CCNNNNNNNGG 4 cut(s) 277, 688, 730, 1162
Bse1I ACTGG 4 cut(s) 282, 663, 970, 1291
Bse3DI GCAATG 4 cut(s) 128, 318, 756, 1278
BseAI TCCGGA 1 cut(s) 1155
BseBI CCWGG 2 cut(s) 959, 1163
BseDI CCNNGG 3 cut(s) 885, 957, 958
BseGI GGATG 5 cut(s) 231, 286, 685, 739, 1174
BseLI CCNNNNNNNGG 4 cut(s) 277, 688, 730, 1162
BseMI GCAATG 4 cut(s) 128, 318, 756, 1278
BseMII CTCAG 2 cut(s) 167, 214
BseNI ACTGG 4 cut(s) 282, 663, 970, 1291
BseRI GAGGAG 4 cut(s) 228, 231, 479, 780
BseSI GKGCMC 1 cut(s) 812
BseXI GCAGC 5 cut(s) 179, 407, 782, 857, 1062
BseYI CCCAGC 1 cut(s) 1238
BsgI GTGCAG 1 cut(s) 629
Bsh1236I CGCG 1 cut(s) 1008
Bsh1285I CGRYCG 1 cut(s) 693
BshFI GGCC 2 cut(s) 239, 342
BsiEI CGRYCG 1 cut(s) 693
BsiSI CCGG 5 cut(s) 236, 324, 689, 1134, 1156
BslFI GGGAC 3 cut(s) 582, 940, 1255
BslI CCNNNNNNNGG 4 cut(s) 277, 688, 730, 1162
BsmAI GTCTC 2 cut(s) 714, 1135
BsmFI GGGAC 3 cut(s) 582, 940, 1255
BsnI GGCC 2 cut(s) 239, 342
Bso31I GGTCTC 1 cut(s) 714
Bsp1286I GDGCHC 1 cut(s) 812
Bsp13I TCCGGA 1 cut(s) 1155
Bsp143I GATC 3 cut(s) 603, 968, 1158
BspACI CCGC 1 cut(s) 1008
BspANI GGCC 2 cut(s) 239, 342
BspCNI CTCAG 2 cut(s) 166, 213
BspEI TCCGGA 1 cut(s) 1155
BspFNI CGCG 1 cut(s) 1008
BspLI GGNNCC 5 cut(s) 328, 432, 468, 570, 1160
BspMAI CTGCAG 1 cut(s) 772
BspPI GGATC 3 cut(s) 976, 1153, 1166
BspTNI GGTCTC 1 cut(s) 714
BsrDI GCAATG 4 cut(s) 128, 318, 756, 1278
BsrI ACTGG 4 cut(s) 282, 663, 970, 1291
BssECI CCNNGG 3 cut(s) 885, 957, 958
BssMI GATC 3 cut(s) 603, 968, 1158
BssNAI GTATAC 1 cut(s) 1108
BssT1I CCWWGG 1 cut(s) 885
Bst1107I GTATAC 1 cut(s) 1108
Bst2UI CCWGG 2 cut(s) 959, 1163
Bst4CI ACNGT 5 cut(s) 254, 616, 777, 1264, 1359
Bst6I CTCTTC 2 cut(s) 420, 515
BstAPI GCANNNNNTGC 2 cut(s) 299, 1346
BstBAI YACGTR 1 cut(s) 1364
BstC8I GCNNGC 4 cut(s) 528, 867, 923, 1380
BstDEI CTNAG 3 cut(s) 153, 200, 743
BstF5I GGATG 5 cut(s) 231, 286, 685, 739, 1174
BstFNI CGCG 1 cut(s) 1008
BstH2I RGCGCY 1 cut(s) 922
BstHHI GCGC 1 cut(s) 921
BstKTI GATC 3 cut(s) 606, 971, 1161
BstMAI GTCTC 2 cut(s) 714, 1135
BstMBI GATC 3 cut(s) 603, 968, 1158
BstMCI CGRYCG 1 cut(s) 693
BstMWI GCNNNNNNNGC 4 cut(s) 299, 339, 767, 1346
BstNI CCWGG 2 cut(s) 959, 1163
BstNSI RCATGY 1 cut(s) 1320
BstSCI CCNGG 3 cut(s) 234, 957, 1161
BstSFI CTRYAG 1 cut(s) 768
BstSLI GKGCMC 1 cut(s) 812
BstUI CGCG 1 cut(s) 1008
BstV1I GCAGC 5 cut(s) 179, 407, 782, 857, 1062
BstV2I GAAGAC 1 cut(s) 688
BstX2I RGATCY 2 cut(s) 968, 1158
BstXI CCANNNNNNTGG 1 cut(s) 1169
BstYI RGATCY 2 cut(s) 968, 1158
BstZ17I GTATAC 1 cut(s) 1108
BsuI GTATCC 1 cut(s) 1067
BsuRI GGCC 2 cut(s) 239, 342
BtgZI GCGATG 1 cut(s) 1001
BtrI CACGTC 1 cut(s) 62
BtsCI GGATG 5 cut(s) 231, 286, 685, 739, 1174
BtsIMutI CAGTG 2 cut(s) 1273, 1284
Cac8I GCNNGC 4 cut(s) 528, 867, 923, 1380
CaiI CAGNNNCTG 1 cut(s) 277
CfoI GCGC 1 cut(s) 921
Cfr13I GGNCC 1 cut(s) 569
CseI GACGC 1 cut(s) 1340
Csp6I GTAC 1 cut(s) 679
CspCI CAANNNNNGTGG 6 cut(s) 229, 264, 441, 476, 847, 882
CviAII CATG 5 cut(s) 355, 1096, 1312, 1317, 1389
CviQI GTAC 1 cut(s) 679
DdeI CTNAG 3 cut(s) 153, 200, 743
DpnI GATC 3 cut(s) 605, 970, 1160
DpnII GATC 3 cut(s) 603, 968, 1158
EaeI YGGCCR 1 cut(s) 237
Eam1104I CTCTTC 2 cut(s) 420, 515
EarI CTCTTC 2 cut(s) 420, 515
Eco130I CCWWGG 1 cut(s) 885
Eco147I AGGCCT 1 cut(s) 342
Eco31I GGTCTC 1 cut(s) 714
Eco47I GGWCC 1 cut(s) 569
Eco72I CACGTG 1 cut(s) 1364
EcoRII CCWGG 2 cut(s) 957, 1161
EcoT14I CCWWGG 1 cut(s) 885
ErhI CCWWGG 1 cut(s) 885
FaeI CATG 5 cut(s) 358, 1099, 1315, 1320, 1392
FaqI GGGAC 3 cut(s) 582, 940, 1255
FatI CATG 5 cut(s) 354, 1095, 1311, 1316, 1388
FblI GTMKAC 1 cut(s) 1107
Fnu4HI GCNGC 6 cut(s) 168, 396, 771, 846, 1009, 1051
FokI GGATG 5 cut(s) 218, 293, 672, 746, 1181
Fsp4HI GCNGC 6 cut(s) 168, 396, 771, 846, 1009, 1051
FspBI CTAG 1 cut(s) 827
GlaI GCGC 1 cut(s) 920
GluI GCNGC 6 cut(s) 168, 396, 771, 846, 1009, 1051
GsaI CCCAGC 1 cut(s) 1242
GsuI CTGGAG 1 cut(s) 254
HaeII RGCGCY 1 cut(s) 922
HaeIII GGCC 2 cut(s) 239, 342
HapII CCGG 5 cut(s) 236, 324, 689, 1134, 1156
HgaI GACGC 1 cut(s) 1340
HhaI GCGC 1 cut(s) 921
Hin1II CATG 5 cut(s) 358, 1099, 1315, 1320, 1392
Hin6I GCGC 1 cut(s) 919
HinP1I GCGC 1 cut(s) 919
HincII GTYRAC 1 cut(s) 1093
HindII GTYRAC 1 cut(s) 1093
HindIII AAGCTT 2 cut(s) 17, 941
HinfI GANTC 6 cut(s) 260, 631, 1020, 1039, 1081, 1202
HpaII CCGG 5 cut(s) 236, 324, 689, 1134, 1156
HphI GGTGA 3 cut(s) 296, 352, 1048
Hpy166II GTNNAC 2 cut(s) 1093, 1108
Hpy188I TCNGA 7 cut(s) 7, 66, 156, 265, 898, 1001, 1141
Hpy188III TCNNGA 7 cut(s) 257, 271, 499, 635, 1024, 1156, 1211
Hpy8I GTNNAC 2 cut(s) 1093, 1108
HpyAV CCTTC 3 cut(s) 703, 973, 1228
HpyCH4III ACNGT 5 cut(s) 254, 616, 777, 1264, 1359
HpyCH4IV ACGT 2 cut(s) 61, 1363
HpyF10VI GCNNNNNNNGC 4 cut(s) 299, 339, 767, 1346
HpyF3I CTNAG 3 cut(s) 153, 200, 743
HpySE526I ACGT 2 cut(s) 61, 1363
Hsp92II CATG 5 cut(s) 358, 1099, 1315, 1320, 1392
HspAI GCGC 1 cut(s) 919
Kpn2I TCCGGA 1 cut(s) 1155
Kzo9I GATC 3 cut(s) 603, 968, 1158
LmnI GCTCC 3 cut(s) 138, 436, 466
Lsp1109I GCAGC 5 cut(s) 179, 407, 782, 857, 1062
LweI GCATC 3 cut(s) 237, 280, 978
MaeI CTAG 1 cut(s) 827
MaeII ACGT 2 cut(s) 61, 1363
MaeIII GTNAC 1 cut(s) 1359
MalI GATC 3 cut(s) 605, 970, 1160
MboI GATC 3 cut(s) 603, 968, 1158
MboII GAAGA 7 cut(s) 299, 433, 437, 514, 532, 688, 1114
MfeI CAATTG 1 cut(s) 1335
MflI RGATCY 2 cut(s) 968, 1158
MhlI GDGCHC 1 cut(s) 812
MluCI AATT 9 cut(s) 195, 413, 437, 505, 797, 1065, 1147, 1188, 1335
MlyI GAGTC 1 cut(s) 1075
MmeI TCCRAC 2 cut(s) 598, 979
MroI TCCGGA 1 cut(s) 1155
MseI TTAA 4 cut(s) 80, 174, 644, 1064
MslI CAYNNNNRTG 1 cut(s) 353
MspI CCGG 5 cut(s) 236, 324, 689, 1134, 1156
MspR9I CCNGG 3 cut(s) 236, 959, 1163
MunI CAATTG 1 cut(s) 1335
MvaI CCWGG 2 cut(s) 959, 1163
MvnI CGCG 1 cut(s) 1008
MwoI GCNNNNNNNGC 4 cut(s) 299, 339, 767, 1346
NciI CCSGG 1 cut(s) 236
NdeII GATC 3 cut(s) 603, 968, 1158
NlaIII CATG 5 cut(s) 358, 1099, 1315, 1320, 1392
NlaIV GGNNCC 5 cut(s) 328, 432, 468, 570, 1160
NmuCI GTSAC 1 cut(s) 1359
NspI RCATGY 1 cut(s) 1320
PasI CCCWGGG 1 cut(s) 958
PceI AGGCCT 1 cut(s) 342
PcsI WCGNNNNNNNCGW 1 cut(s) 612
PfeI GAWTC 5 cut(s) 260, 631, 1020, 1039, 1202
PflFI GACNNNGTC 1 cut(s) 827
PflMI CCANNNNNTGG 1 cut(s) 277
PfoI TCCNGGA 1 cut(s) 1161
PkrI GCNGC 6 cut(s) 169, 397, 772, 847, 1010, 1052
PleI GAGTC 1 cut(s) 1075
PmaCI CACGTG 1 cut(s) 1364
PmlI CACGTG 1 cut(s) 1364
PpsI GAGTC 1 cut(s) 1075
Ppu21I YACGTR 1 cut(s) 1364
PsiI TTATAA 1 cut(s) 111
Psp6I CCWGG 2 cut(s) 957, 1161
PspCI CACGTG 1 cut(s) 1364
PspFI CCCAGC 1 cut(s) 1238
PspGI CCWGG 2 cut(s) 957, 1161
PspN4I GGNNCC 5 cut(s) 328, 432, 468, 570, 1160
PspPI GGNCC 1 cut(s) 569
PstI CTGCAG 1 cut(s) 772
PstNI CAGNNNCTG 1 cut(s) 277
PsuI RGATCY 2 cut(s) 968, 1158
PsyI GACNNNGTC 1 cut(s) 827
RsaI GTAC 1 cut(s) 680
RsaNI GTAC 1 cut(s) 679
RseI CAYNNNNRTG 1 cut(s) 353
SaqAI TTAA 4 cut(s) 80, 174, 644, 1064
SatI GCNGC 6 cut(s) 168, 396, 771, 846, 1009, 1051
Sau3AI GATC 3 cut(s) 603, 968, 1158
Sau96I GGNCC 1 cut(s) 569
SchI GAGTC 1 cut(s) 1075
ScrFI CCNGG 3 cut(s) 236, 959, 1163
SduI GDGCHC 1 cut(s) 812
SfaNI GCATC 3 cut(s) 237, 280, 978
SfcI CTRYAG 1 cut(s) 768
SinI GGWCC 1 cut(s) 569
SmiMI CAYNNNNRTG 1 cut(s) 353
Sse9I AATT 9 cut(s) 195, 413, 437, 505, 797, 1065, 1147, 1188, 1335
SseBI AGGCCT 1 cut(s) 342
SsiI CCGC 1 cut(s) 1008
SspMI CTAG 1 cut(s) 827
StuI AGGCCT 1 cut(s) 342
StyD4I CCNGG 3 cut(s) 234, 957, 1161
StyI CCWWGG 1 cut(s) 885
TaaI ACNGT 5 cut(s) 254, 616, 777, 1264, 1359
TaiI ACGT 2 cut(s) 64, 1366
TaqI TCGA 5 cut(s) 312, 579, 634, 831, 1281
TasI AATT 9 cut(s) 195, 413, 437, 505, 797, 1065, 1147, 1188, 1335
TauI GCSGC 1 cut(s) 1011
TfiI GAWTC 5 cut(s) 260, 631, 1020, 1039, 1202
Tru1I TTAA 4 cut(s) 80, 174, 644, 1064
Tru9I TTAA 4 cut(s) 80, 174, 644, 1064
TscAI CASTG 2 cut(s) 1273, 1291
TseFI GTSAC 1 cut(s) 1359
TseI GCWGC 5 cut(s) 167, 395, 770, 845, 1050
Tsp45I GTSAC 1 cut(s) 1359
TspDTI ATGAA 6 cut(s) 35, 307, 387, 689, 1200, 1289
TspGWI ACGGA 1 cut(s) 225
TspRI CASTG 2 cut(s) 1273, 1291
Tth111I GACNNNGTC 1 cut(s) 827
Van91I CCANNNNNTGG 1 cut(s) 277
VpaK11BI GGWCC 1 cut(s) 569
XapI RAATTY 4 cut(s) 413, 437, 505, 797
XceI RCATGY 1 cut(s) 1320
XcmI CCANNNNNNNNNTGG 1 cut(s) 278
XmiI GTMKAC 1 cut(s) 1107
XspI CTAG 1 cut(s) 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.