pycom11g08410

lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0022434
Physical Location & Seq
Forward (+)
6525290 .. 6525920
631 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g08410.2

Sequence Viewer

Length: 333 bp
ATGCTGGAAGTCGGAAATCCCAACGAATGGGGAATCTTGAATTGGTTCATGAATTCAAAAAGAAGCGGTATCCCTTTAATTGATGTTCTTACGACCCCAAGTGTCAACATGGTTGAAGTATACCTGTCTGCTTTCTTCAATATCTCCGGCTCTGATGGCAATTATCTCCGGATCCAGTCTGGGACTAACCTCCTTGAAAAAACTGTCAGTGCAATGAACCTCGACACTGGCTGGTATGATGAACCAATTGACATGACATGCAAATACAAAGACGCAATTGCAGAATTTGCCCAAAAACTGTCACGTGAAAAAAAGAGGCGAGCAAATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

111

Amino Acids

12.47

Weight (kDa)

5.38

Isoelectric Point (pI)

30.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022434)

Species Orthologous Gene IDs
malus_domestica MD11G1099600.v1.1
pyrus_communis pycom11g08380 pycom11g08390 pycom11g08410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 27
AccI GTMKAC 1 cut(s) 120
AccIII TCCGGA 1 cut(s) 168
AciI CCGC 1 cut(s) 66
AclWI GGATC 2 cut(s) 166, 179
AcsI RAATTY 2 cut(s) 52, 284
AcvI CACGTG 1 cut(s) 305
AfiI CCNNNNNNNGG 1 cut(s) 27
AgsI TTSAA 5 cut(s) 40, 57, 116, 139, 197
AjuI GAANNNNNNNTTGG 2 cut(s) 25, 57
AlwI GGATC 2 cut(s) 166, 179
Aor13HI TCCGGA 1 cut(s) 168
ApoI RAATTY 2 cut(s) 52, 284
Asp700I GAANNNNTTC 1 cut(s) 44
BamHI GGATCC 1 cut(s) 171
BbrPI CACGTG 1 cut(s) 305
BccI CCATC 1 cut(s) 149
BciVI GTATCC 1 cut(s) 80
BfuI GTATCC 1 cut(s) 80
BmiI GGNNCC 1 cut(s) 173
BsaAI YACGTR 1 cut(s) 305
BsaWI WCCGGW 1 cut(s) 168
Bsc4I CCNNNNNNNGG 1 cut(s) 27
Bse1I ACTGG 2 cut(s) 175, 232
Bse3DI GCAATG 1 cut(s) 219
BseAI TCCGGA 1 cut(s) 168
BseLI CCNNNNNNNGG 1 cut(s) 27
BseMI GCAATG 1 cut(s) 219
BseNI ACTGG 2 cut(s) 175, 232
BsiSI CCGG 2 cut(s) 147, 169
BslFI GGGAC 1 cut(s) 196
BslI CCNNNNNNNGG 1 cut(s) 27
BsmFI GGGAC 1 cut(s) 196
Bsp13I TCCGGA 1 cut(s) 168
Bsp143I GATC 1 cut(s) 171
BspACI CCGC 1 cut(s) 66
BspEI TCCGGA 1 cut(s) 168
BspHI TCATGA 1 cut(s) 48
BspLI GGNNCC 1 cut(s) 173
BspPI GGATC 2 cut(s) 166, 179
BsrDI GCAATG 1 cut(s) 219
BsrI ACTGG 2 cut(s) 175, 232
BssMI GATC 1 cut(s) 171
BssNAI GTATAC 1 cut(s) 121
Bst1107I GTATAC 1 cut(s) 121
Bst4CI ACNGT 2 cut(s) 205, 300
BstAPI GCANNNNNTGC 1 cut(s) 287
BstBAI YACGTR 1 cut(s) 305
BstC8I GCNNGC 1 cut(s) 321
BstKTI GATC 1 cut(s) 174
BstMBI GATC 1 cut(s) 171
BstMWI GCNNNNNNNGC 2 cut(s) 156, 287
BstNSI RCATGY 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 171
BstYI RGATCY 1 cut(s) 171
BstZ17I GTATAC 1 cut(s) 121
BsuI GTATCC 1 cut(s) 80
BtsIMutI CAGTG 2 cut(s) 214, 225
Cac8I GCNNGC 1 cut(s) 321
CciI TCATGA 1 cut(s) 48
CseI GACGC 1 cut(s) 281
CviAII CATG 5 cut(s) 49, 109, 253, 258, 330
CviJI RGCY 2 cut(s) 150, 231
CviKI_1 RGCY 2 cut(s) 150, 231
DpnI GATC 1 cut(s) 173
DpnII GATC 1 cut(s) 171
Eco72I CACGTG 1 cut(s) 305
EcoRI GAATTC 1 cut(s) 52
FaeI CATG 5 cut(s) 52, 112, 256, 261, 333
FaiI YATR 7 cut(s) 50, 110, 121, 237, 254, 259, 331
FaqI GGGAC 1 cut(s) 196
FatI CATG 5 cut(s) 48, 108, 252, 257, 329
FblI GTMKAC 1 cut(s) 120
HapII CCGG 2 cut(s) 147, 169
HgaI GACGC 1 cut(s) 281
Hin1II CATG 5 cut(s) 52, 112, 256, 261, 333
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HinfI GANTC 1 cut(s) 33
HpaII CCGG 2 cut(s) 147, 169
Hpy166II GTNNAC 2 cut(s) 106, 121
Hpy188I TCNGA 2 cut(s) 14, 154
Hpy188III TCNNGA 3 cut(s) 37, 49, 169
Hpy8I GTNNAC 2 cut(s) 106, 121
HpyCH4III ACNGT 2 cut(s) 205, 300
HpyCH4IV ACGT 1 cut(s) 304
HpyCH4V TGCA 3 cut(s) 212, 261, 281
HpyF10VI GCNNNNNNNGC 2 cut(s) 156, 287
HpySE526I ACGT 1 cut(s) 304
Hsp92II CATG 5 cut(s) 52, 112, 256, 261, 333
Kpn2I TCCGGA 1 cut(s) 168
Kzo9I GATC 1 cut(s) 171
LpnPI CCDG 7 cut(s) 137, 160, 165, 182, 188, 213, 217
MaeII ACGT 1 cut(s) 304
MaeIII GTNAC 1 cut(s) 300
MalI GATC 1 cut(s) 173
MboI GATC 1 cut(s) 171
MboII GAAGA 1 cut(s) 127
MfeI CAATTG 2 cut(s) 246, 276
MflI RGATCY 1 cut(s) 171
MluCI AATT 7 cut(s) 40, 52, 78, 160, 246, 276, 284
MnlI CCTC 3 cut(s) 200, 230, 309
MroI TCCGGA 1 cut(s) 168
MroXI GAANNNNTTC 1 cut(s) 44
MseI TTAA 1 cut(s) 77
MspI CCGG 2 cut(s) 147, 169
MunI CAATTG 2 cut(s) 246, 276
MwoI GCNNNNNNNGC 2 cut(s) 156, 287
NdeII GATC 1 cut(s) 171
NlaIII CATG 5 cut(s) 52, 112, 256, 261, 333
NlaIV GGNNCC 1 cut(s) 173
NmuCI GTSAC 1 cut(s) 300
NspI RCATGY 1 cut(s) 261
PagI TCATGA 1 cut(s) 48
PdmI GAANNNNTTC 1 cut(s) 44
PfeI GAWTC 1 cut(s) 33
PflMI CCANNNNNTGG 1 cut(s) 27
PmaCI CACGTG 1 cut(s) 305
PmlI CACGTG 1 cut(s) 305
Ppu21I YACGTR 1 cut(s) 305
PspCI CACGTG 1 cut(s) 305
PspN4I GGNNCC 1 cut(s) 173
PsuI RGATCY 1 cut(s) 171
SaqAI TTAA 1 cut(s) 77
Sau3AI GATC 1 cut(s) 171
SetI ASST 4 cut(s) 126, 192, 222, 307
Sse9I AATT 7 cut(s) 40, 52, 78, 160, 246, 276, 284
SsiI CCGC 1 cut(s) 66
TaaI ACNGT 2 cut(s) 205, 300
TaiI ACGT 1 cut(s) 307
TaqI TCGA 1 cut(s) 222
TasI AATT 7 cut(s) 40, 52, 78, 160, 246, 276, 284
TfiI GAWTC 1 cut(s) 33
Tru1I TTAA 1 cut(s) 77
Tru9I TTAA 1 cut(s) 77
TscAI CASTG 2 cut(s) 214, 232
TseFI GTSAC 1 cut(s) 300
Tsp45I GTSAC 1 cut(s) 300
TspDTI ATGAA 4 cut(s) 37, 65, 230, 255
TspRI CASTG 2 cut(s) 214, 232
Van91I CCANNNNNTGG 1 cut(s) 27
XapI RAATTY 2 cut(s) 52, 284
XceI RCATGY 1 cut(s) 261
XmiI GTMKAC 1 cut(s) 120
XmnI GAANNNNTTC 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.