MD11G1100600.v1.1

aminoacylase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0028917
Physical Location & Seq
Reverse (-)
8422492 .. 8430212
7721 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1100600.v1.1.491

Sequence Viewer

Length: 450 bp
ATGGGAGATCCAATGAAAAAAAAAACAGCAACAGATCAACCGAGGTCTATAGATTCCAACTGCCTCACAACTGCCGCCAGAGAGGTCAGATTGTCCAGAGCGCTGCCACTTCCTCCGCCAACACCGCTGTGTCAGATGTTAAACTGCAGCTCCAGGAGGAGGACCGCAGCAATGGCTTCAATTTCCACTTTTGCTTCTGCGTGGTCCGAATTTGCCAGGAATGTGTCCGGTGAATGGGATGGCCATGGAGCAGACTTCACAAGGGAAGGGTGCCCAATAGAACTTCCAGAGAATGTTGTACCTACAGCTTACAGGGAGTCGGAGGTTAAGGTTTTCGATTGGCAGACTCAATGCCCCACTCTTGCTAACCCAGATGAGCACACTCTTTTACACAAGAATATAGATTTACTTCCTGCCGTTGGATGTGAAGCTGATGCTGACACTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.37

Weight (kDa)

5.15

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028917)

Species Orthologous Gene IDs
malus_domestica MD11G1100600.v1.1
rosa_multiflora Rmu_ssc0000125.1_g000025

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 270
AciI CCGC 4 cut(s) 75, 116, 125, 165
AclWI GGATC 1 cut(s) 2
AcoI YGGCCR 1 cut(s) 241
AcsI RAATTY 1 cut(s) 209
AfaI GTAC 1 cut(s) 300
AfeI AGCGCT 1 cut(s) 102
AfiI CCNNNNNNNGG 3 cut(s) 159, 234, 419
AgsI TTSAA 1 cut(s) 180
AjnI CCWGG 2 cut(s) 152, 215
AleI CACNNNNGTG 1 cut(s) 127
AluBI AGCT 3 cut(s) 150, 308, 431
AluI AGCT 3 cut(s) 150, 308, 431
Alw21I GWGCWC 1 cut(s) 381
AlwI GGATC 1 cut(s) 2
Aor51HI AGCGCT 1 cut(s) 102
AoxI GGCC 1 cut(s) 241
ApeKI GCWGC 3 cut(s) 103, 147, 167
ApoI RAATTY 1 cut(s) 209
AspLEI GCGC 1 cut(s) 103
AspS9I GGNCC 2 cut(s) 162, 204
AsuHPI GGTGA 1 cut(s) 242
AvaII GGWCC 2 cut(s) 162, 204
BaeGI GKGCMC 1 cut(s) 275
BalI TGGCCA 1 cut(s) 243
BanI GGYRCC 1 cut(s) 270
Bbv12I GWGCWC 1 cut(s) 381
BbvI GCAGC 3 cut(s) 90, 159, 179
BccI CCATC 1 cut(s) 233
BceAI ACGGC 1 cut(s) 401
BciT130I CCWGG 2 cut(s) 154, 217
BfmI CTRYAG 3 cut(s) 48, 145, 303
BfoI RGCGCY 1 cut(s) 104
BisI GCNGC 4 cut(s) 75, 104, 148, 168
BlsI GCNGC 4 cut(s) 76, 105, 149, 169
Bme1390I CCNGG 2 cut(s) 154, 217
Bme18I GGWCC 2 cut(s) 162, 204
BmgT120I GGNCC 2 cut(s) 162, 204
BmiI GGNNCC 1 cut(s) 272
BmrFI CCNGG 2 cut(s) 154, 217
BmsI GCATC 1 cut(s) 424
BpmI CTGGAG 1 cut(s) 136
BsaJI CCNNGG 2 cut(s) 41, 244
BsaWI WCCGGW 1 cut(s) 227
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 234, 419
Bse3DI GCAATG 1 cut(s) 177
BseBI CCWGG 2 cut(s) 154, 217
BseDI CCNNGG 2 cut(s) 41, 244
BseGI GGATG 2 cut(s) 244, 428
BseLI CCNNNNNNNGG 3 cut(s) 159, 234, 419
BseMI GCAATG 1 cut(s) 177
BseRI GAGGAG 1 cut(s) 172
BseSI GKGCMC 1 cut(s) 275
BseXI GCAGC 3 cut(s) 90, 159, 179
BshFI GGCC 1 cut(s) 243
BshNI GGYRCC 1 cut(s) 270
BsiHKAI GWGCWC 1 cut(s) 381
BsiSI CCGG 1 cut(s) 228
BslI CCNNNNNNNGG 3 cut(s) 159, 234, 419
BsnI GGCC 1 cut(s) 243
Bsp1286I GDGCHC 2 cut(s) 275, 381
Bsp143I GATC 2 cut(s) 7, 34
Bsp19I CCATGG 1 cut(s) 244
BspACI CCGC 4 cut(s) 75, 116, 125, 165
BspANI GGCC 1 cut(s) 243
BspLI GGNNCC 1 cut(s) 272
BspMAI CTGCAG 1 cut(s) 149
BspPI GGATC 1 cut(s) 2
BspT107I GGYRCC 1 cut(s) 270
BsrDI GCAATG 1 cut(s) 177
BssECI CCNNGG 2 cut(s) 41, 244
BssMI GATC 2 cut(s) 7, 34
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 2 cut(s) 154, 217
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 2 cut(s) 244, 428
BstH2I RGCGCY 1 cut(s) 104
BstHHI GCGC 1 cut(s) 103
BstKTI GATC 2 cut(s) 10, 37
BstMBI GATC 2 cut(s) 7, 34
BstMWI GCNNNNNNNGC 2 cut(s) 124, 173
BstNI CCWGG 2 cut(s) 154, 217
BstSCI CCNGG 2 cut(s) 152, 215
BstSFI CTRYAG 3 cut(s) 48, 145, 303
BstSLI GKGCMC 1 cut(s) 275
BstV1I GCAGC 3 cut(s) 90, 159, 179
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BsuRI GGCC 1 cut(s) 243
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 2 cut(s) 244, 428
CfoI GCGC 1 cut(s) 103
Cfr13I GGNCC 2 cut(s) 162, 204
Csp6I GTAC 1 cut(s) 299
CviAII CATG 1 cut(s) 245
CviJI RGCY 5 cut(s) 150, 176, 243, 308, 431
CviKI_1 RGCY 5 cut(s) 150, 176, 243, 308, 431
CviQI GTAC 1 cut(s) 299
DpnI GATC 2 cut(s) 9, 36
DpnII GATC 2 cut(s) 7, 34
EaeI YGGCCR 1 cut(s) 241
EciI GGCGGA 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 244
Eco47I GGWCC 2 cut(s) 162, 204
Eco47III AGCGCT 1 cut(s) 102
EcoRII CCWGG 2 cut(s) 152, 215
EcoT14I CCWWGG 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 1 cut(s) 248
FaiI YATR 3 cut(s) 50, 246, 401
FatI CATG 1 cut(s) 244
Fnu4HI GCNGC 4 cut(s) 75, 104, 148, 168
FokI GGATG 2 cut(s) 251, 435
Fsp4HI GCNGC 4 cut(s) 75, 104, 148, 168
GlaI GCGC 1 cut(s) 102
GluI GCNGC 4 cut(s) 75, 104, 148, 168
GsuI CTGGAG 1 cut(s) 136
HaeII RGCGCY 1 cut(s) 104
HaeIII GGCC 1 cut(s) 243
HapII CCGG 1 cut(s) 228
HhaI GCGC 1 cut(s) 103
Hin1II CATG 1 cut(s) 248
Hin6I GCGC 1 cut(s) 101
HinP1I GCGC 1 cut(s) 101
HinfI GANTC 3 cut(s) 53, 317, 346
HpaII CCGG 1 cut(s) 228
HphI GGTGA 1 cut(s) 242
Hpy188I TCNGA 4 cut(s) 89, 135, 208, 322
Hpy188III TCNNGA 2 cut(s) 96, 287
HpyAV CCTTC 1 cut(s) 260
HpyCH4V TGCA 1 cut(s) 147
HpyF10VI GCNNNNNNNGC 2 cut(s) 124, 173
Hsp92II CATG 1 cut(s) 248
HspAI GCGC 1 cut(s) 101
Kzo9I GATC 2 cut(s) 7, 34
LmnI GCTCC 2 cut(s) 155, 248
Lsp1109I GCAGC 3 cut(s) 90, 159, 179
LweI GCATC 1 cut(s) 424
MalI GATC 2 cut(s) 9, 36
MboI GATC 2 cut(s) 7, 34
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 2 cut(s) 275, 381
MlsI TGGCCA 1 cut(s) 243
MluCI AATT 2 cut(s) 180, 209
MluNI TGGCCA 1 cut(s) 243
MlyI GAGTC 2 cut(s) 326, 340
MmeI TCCRAC 3 cut(s) 81, 300, 400
MnlI CCTC 7 cut(s) 36, 74, 76, 123, 150, 153, 316
Mox20I TGGCCA 1 cut(s) 243
MscI TGGCCA 1 cut(s) 243
MseI TTAA 2 cut(s) 140, 327
MslI CAYNNNNRTG 1 cut(s) 127
Msp20I TGGCCA 1 cut(s) 243
MspA1I CMGCKG 1 cut(s) 127
MspI CCGG 1 cut(s) 228
MspR9I CCNGG 2 cut(s) 154, 217
MvaI CCWGG 2 cut(s) 154, 217
MwoI GCNNNNNNNGC 2 cut(s) 124, 173
NcoI CCATGG 1 cut(s) 244
NdeII GATC 2 cut(s) 7, 34
NlaIII CATG 1 cut(s) 248
NlaIV GGNNCC 1 cut(s) 272
OliI CACNNNNGTG 1 cut(s) 127
PfeI GAWTC 1 cut(s) 53
PfoI TCCNGGA 1 cut(s) 152
PkrI GCNGC 4 cut(s) 76, 105, 149, 169
PleI GAGTC 2 cut(s) 325, 340
PpsI GAGTC 2 cut(s) 325, 340
Psp6I CCWGG 2 cut(s) 152, 215
PspGI CCWGG 2 cut(s) 152, 215
PspN4I GGNNCC 1 cut(s) 272
PspPI GGNCC 2 cut(s) 162, 204
PstI CTGCAG 1 cut(s) 149
PsuI RGATCY 1 cut(s) 7
RsaI GTAC 1 cut(s) 300
RsaNI GTAC 1 cut(s) 299
RseI CAYNNNNRTG 1 cut(s) 127
SaqAI TTAA 2 cut(s) 140, 327
SatI GCNGC 4 cut(s) 75, 104, 148, 168
Sau3AI GATC 2 cut(s) 7, 34
Sau96I GGNCC 2 cut(s) 162, 204
SchI GAGTC 2 cut(s) 326, 340
ScrFI CCNGG 2 cut(s) 154, 217
SduI GDGCHC 2 cut(s) 275, 381
SetI ASST 8 cut(s) 47, 87, 152, 304, 310, 327, 333, 433
SfaNI GCATC 1 cut(s) 424
SfcI CTRYAG 3 cut(s) 48, 145, 303
SinI GGWCC 2 cut(s) 162, 204
SmiMI CAYNNNNRTG 1 cut(s) 127
Sse9I AATT 2 cut(s) 180, 209
SsiI CCGC 4 cut(s) 75, 116, 125, 165
StyD4I CCNGG 2 cut(s) 152, 215
StyI CCWWGG 1 cut(s) 244
TaqI TCGA 1 cut(s) 336
TasI AATT 2 cut(s) 180, 209
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 1 cut(s) 53
Tru1I TTAA 2 cut(s) 140, 327
Tru9I TTAA 2 cut(s) 140, 327
TseI GCWGC 3 cut(s) 103, 147, 167
TspDTI ATGAA 1 cut(s) 29
VpaK11BI GGWCC 2 cut(s) 162, 204
XapI RAATTY 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.