MD11G1263400.v1.1
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0024493
Physical Location & Seq
Forward (+)
37863262 .. 37863707
446 bp
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UTR
Exon/CDS
Intron
MD11G1263400.v1.1.491

Sequence Viewer

Length: 255 bp
ATGGGTTCAAAATTTAGTCATGGAATTACGTGCTTTTTGTTTTTGGTAACAATATTTGCCATCAAAGCCAAAACGGATGGTATCGATCTTGACATAAAAAAATTTGGGGCCAAGGGTGACGGCAAGACAGGTGATACTGCTGCTTTTTCAAATGCATGGCAAGAAGCATGCAGATCCAAGAAAGAGAGCGTCCTTACAATACCAAAAGGAACATATATAGTCGGTAAACTTGAATTTATGGGACCCTGCGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

85

Amino Acids

9.07

Weight (kDa)

8.84

Isoelectric Point (pI)

5.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 32 - 75 1.4e-07 Rhamnogalacturonase A/epimerase, pectate lyase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024493)

Species Orthologous Gene IDs
malus_domestica MD11G1263400.v1.1
prunus_persica Prupe.4G165000_v2.0.a1 Prupe.4G165100_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 168
AcsI RAATTY 3 cut(s) 11, 101, 233
AgsI TTSAA 3 cut(s) 9, 150, 233
AlwI GGATC 1 cut(s) 168
AoxI GGCC 1 cut(s) 108
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 3 cut(s) 11, 101, 233
AspS9I GGNCC 2 cut(s) 108, 242
AsuHPI GGTGA 2 cut(s) 128, 143
AvaII GGWCC 1 cut(s) 242
BbvI GCAGC 1 cut(s) 127
BccI CCATC 2 cut(s) 68, 71
BceAI ACGGC 1 cut(s) 136
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
Bme18I GGWCC 1 cut(s) 242
BmgT120I GGNCC 2 cut(s) 108, 242
BmiI GGNNCC 3 cut(s) 109, 243, 244
Bsa29I ATCGAT 1 cut(s) 84
BsaAI YACGTR 1 cut(s) 30
BsaJI CCNNGG 1 cut(s) 111
BseCI ATCGAT 1 cut(s) 84
BseDI CCNNGG 1 cut(s) 111
BseGI GGATG 1 cut(s) 82
BseXI GCAGC 1 cut(s) 127
BshFI GGCC 1 cut(s) 110
BshVI ATCGAT 1 cut(s) 84
BsnI GGCC 1 cut(s) 110
Bsp143I GATC 2 cut(s) 85, 173
BspANI GGCC 1 cut(s) 110
BspDI ATCGAT 1 cut(s) 84
BspLI GGNNCC 3 cut(s) 109, 243, 244
BspPI GGATC 1 cut(s) 168
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 2 cut(s) 85, 173
BssT1I CCWWGG 1 cut(s) 111
BstBAI YACGTR 1 cut(s) 30
BstC8I GCNNGC 1 cut(s) 169
BstF5I GGATG 1 cut(s) 82
BstKTI GATC 2 cut(s) 88, 176
BstMBI GATC 2 cut(s) 85, 173
BstMWI GCNNNNNNNGC 1 cut(s) 65
BstNSI RCATGY 1 cut(s) 171
BstV1I GCAGC 1 cut(s) 127
BstX2I RGATCY 1 cut(s) 173
BstYI RGATCY 1 cut(s) 173
Bsu15I ATCGAT 1 cut(s) 84
BsuRI GGCC 1 cut(s) 110
BsuTUI ATCGAT 1 cut(s) 84
BtsCI GGATG 1 cut(s) 82
Cac8I GCNNGC 1 cut(s) 169
Cfr13I GGNCC 2 cut(s) 108, 242
ClaI ATCGAT 1 cut(s) 84
CseI GACGC 1 cut(s) 178
CviAII CATG 3 cut(s) 20, 156, 168
CviJI RGCY 2 cut(s) 68, 110
CviKI_1 RGCY 2 cut(s) 68, 110
DpnI GATC 2 cut(s) 87, 175
DpnII GATC 2 cut(s) 85, 173
Eco130I CCWWGG 1 cut(s) 111
Eco47I GGWCC 1 cut(s) 242
EcoO109I RGGNCCY 1 cut(s) 242
EcoT14I CCWWGG 1 cut(s) 111
EcoT22I ATGCAT 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 3 cut(s) 23, 159, 171
FaiI YATR 8 cut(s) 21, 95, 157, 169, 214, 216, 218, 239
FatI CATG 3 cut(s) 19, 155, 167
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 1 cut(s) 89
Fsp4HI GCNGC 1 cut(s) 141
GluI GCNGC 1 cut(s) 141
HaeIII GGCC 1 cut(s) 110
HgaI GACGC 1 cut(s) 178
Hin1II CATG 3 cut(s) 23, 159, 171
HphI GGTGA 2 cut(s) 128, 143
Hpy166II GTNNAC 1 cut(s) 227
Hpy188III TCNNGA 1 cut(s) 89
Hpy8I GTNNAC 1 cut(s) 227
HpyCH4IV ACGT 1 cut(s) 29
HpyCH4V TGCA 2 cut(s) 155, 171
HpyF10VI GCNNNNNNNGC 1 cut(s) 65
HpySE526I ACGT 1 cut(s) 29
Hsp92II CATG 3 cut(s) 23, 159, 171
KflI GGGWCCC 1 cut(s) 242
Kzo9I GATC 2 cut(s) 85, 173
LpnPI CCDG 1 cut(s) 114
Lsp1109I GCAGC 1 cut(s) 127
MaeII ACGT 1 cut(s) 29
MaeIII GTNAC 2 cut(s) 46, 116
MalI GATC 2 cut(s) 87, 175
MboI GATC 2 cut(s) 85, 173
MflI RGATCY 1 cut(s) 173
MluCI AATT 4 cut(s) 11, 24, 101, 233
Mph1103I ATGCAT 1 cut(s) 157
MwoI GCNNNNNNNGC 1 cut(s) 65
NdeII GATC 2 cut(s) 85, 173
NlaIII CATG 3 cut(s) 23, 159, 171
NlaIV GGNNCC 3 cut(s) 109, 243, 244
NmuCI GTSAC 1 cut(s) 116
NsiI ATGCAT 1 cut(s) 157
NspI RCATGY 1 cut(s) 171
PaeI GCATGC 1 cut(s) 171
PkrI GCNGC 1 cut(s) 142
Ppu21I YACGTR 1 cut(s) 30
PpuMI RGGWCCY 1 cut(s) 242
Psp5II RGGWCCY 1 cut(s) 242
PspN4I GGNNCC 3 cut(s) 109, 243, 244
PspPI GGNCC 2 cut(s) 108, 242
PspPPI RGGWCCY 1 cut(s) 242
PsuI RGATCY 1 cut(s) 173
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 2 cut(s) 85, 173
Sau96I GGNCC 2 cut(s) 108, 242
SetI ASST 2 cut(s) 32, 133
SinI GGWCC 1 cut(s) 242
SphI GCATGC 1 cut(s) 171
Sse9I AATT 4 cut(s) 11, 24, 101, 233
SspI AATATT 1 cut(s) 54
StyI CCWWGG 1 cut(s) 111
TaiI ACGT 1 cut(s) 32
TaqI TCGA 1 cut(s) 84
TasI AATT 4 cut(s) 11, 24, 101, 233
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 1 cut(s) 116
TspGWI ACGGA 1 cut(s) 89
VpaK11BI GGWCC 1 cut(s) 242
XapI RAATTY 3 cut(s) 11, 101, 233
XceI RCATGY 1 cut(s) 171
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.