Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0008005

Physical Location & Seq

Genomic Coordinates Forward (+)

751019 .. 756896

Gene Structure 5878 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1006300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1989 bp

ATGATATTCAAATCTAAGATAAAATGGGTTGCTGTCTTTGTCCTCGTTTTATCTTTTGCATCATTACTTCGGCATCTCTCTATAGCACGATTTTCGACTGTCGATTTAGTCCAGTATAGCGCATTGACCGCATTCCATAACGATTTCAATTCTATCTCTGGGAGGCAGAGTGTTCCTAATAAGAAGCTATGGGGGCCTGTGAAGCCCCTGCAGTCTTTGCAGCCTTATGCCAACCCCCGAAGCAACTATCCTGTCCCAAATGACCAGAGCAATGGCTTCATTTACGCAAAAATATTTGGTGGATTTGAAAAGATAAGATCTACGATCTGTGATCTGGTCACCATATCCAGGCTTCTAAATGCTACTCTTGTCATTCCAGACATTCAAGAAAGTACTCGCTCAAAAGGCATCAGCTCTCGTTTCAAGAGTTTTACATATCTCTACGATGAGGTGCACTTTATAGCATCTTTAAAAAATGACATAGTCATTGTGAAGAGTCTGCCAGATAATCTGAAGGCAGCAAGGAAAAGAAATGAATTCCCTTCTTTTAGACCCAAGAAGTCAGCTTCTCCAAATTTTTATATCAAAGAAGTCCTACCAAAGTTGAGGAAAACCAAGGTTATTGGCTTGGTCATTGCTGATGGTGGATGTCTGCAGTCAATCCTTCCACCAAACATGGCCGAGTTTCAAAGACTTAGATGCAGGGTCGCCTTCCATGCACTTCAATTCAGGCCAGAAATCCAAGTGCTTGGACACAAGATGGTGGAGAGGTTACGTGCATGGGGTCAACCTTTCCTAGCGTATCATCCTGGTTTGGTGAGAGAAACCTTGGCATATCATGGCTGTGCTGAACTTTTTCAGGATGTTCACACTGAACTCATACAATATCGAAGGAAACAAATGATTAAAAAGGGCATTGTTCATGAGGAACTAAGCATAGATTCACATTTGCGTAGAGAAAATGGCTCATGCCCGCTGATGCCTGAAGAGGTTGGAATTCTTCTTCGTTCAATGGGGTATCCTCCTAAAACAATTATATATCTGGCTGGTTCTGAAACATTTGGGGGTCAACGTGTTCTGGTCCCTTTGCGTGCTATGTTTCCCAACTTAGTGGATCGCACTTCCTTGTGCAGCAATAAAGAATTGTCTGATATACTTGGACCTGAAACACCCCTTCCGCAAAATCCATTTCAACGACTGCCAGCAAAAAGCAAGGAACAACTTAAAGAAAAATGGAAGAAGGCCGGTCCTAGGCCTCGGCCTCTTCCTCCACCTCCTGGTAGACCTATCTATCAACATGAAAAAGAAGGCTGGTATGGTTGGATTACTGAGTCTGACACGGAACCAGACCTTTCTGCTTTGGATCTGAGGATGCAAGCACATAGACTAATTTGGGATGCTCTTGATTATATTGTCTCTGTGGAAGCAGACACATTCTTTCCTGGTTTTAACAATGATGGCAGTGGATGGCCAGATTTTTCAAGCTTGGTCATGGGACACCGTGTGTATGAGGTTGCTTCTTCTAGAACATACCGACCAGACAGGAAAGTTGCAGCAGAACTTTTCAACATGACCAGGGACAATGTCTACCATCCCAAGCATAATTGGACAGTCTCAGTGCAGGAACATCTTAAGAAAAGCTTAAGTGAGGAAGGCCTAATAAGGCAATCCGTTTTGTCGAAGCCAGCATTATTCATTTCACACCCACTTCCCGAATGCTCATGTAGAATATCTTCTGCCAATGCTCCAGTTCATGTAAAAGGTAATGATGGCCGAACTATATATGGAGGTGAAGAAGAGTGCCCGAAGTGGATGAAGCATGGTGACGAAGTTCCTTTGGATTCAGCTGGGGAAGAGGGTAAGGTGGAGGACAATGAATTGTCAGAGTACGGAAGTACTCTGGATGAACAACCAGAATCAAATGACAGCGACAGAACTAATGCTACTCTTGTCTCTGAGCAGGATGAGGAAATGGACCCGAATGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000741 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006996 GO:0006997 GO:0007275 GO:0008150 GO:0009507 GO:0009536 GO:0009553 GO:0009559 GO:0009561 GO:0009856 GO:0009987 GO:0010197 GO:0012505 GO:0016043 GO:0022414 GO:0030154 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0048229 GO:0048284 GO:0048856 GO:0048868 GO:0048869 GO:0051704 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

O-FucT

Protein Analysis

663

Amino Acids

75.03

Weight (kDa)

8.5

Isoelectric Point (pI)

49.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
O-FucT	PF10250	94 - 390	4.5e-65	GDP-fucose protein O-fucosyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008005)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03810 AT3G03810
fragaria_vesca	FvH4_1g28330 FvH4_1g28330 FvH4_1g28330
malus_domestica	MD12G1006300.v1.1 MD14G1003900.v1.1
prunus_persica	Prupe.7G006400_v2.0.a1 Prupe.7G006400_v2.0.a1 Prupe.7G006400_v2.0.a1 Prupe.7G006400_v2.0.a1 Prupe.7G006400_v2.0.a1 Prupe.7G006400_v2.0.a1
pyrus_communis	pycom12g00460 pycom14g00180
rosa_chinensis	RchiOBHm_Chr3g0496781
rosa_laevigata	RLG00000022504
rosa_multiflora	Rmu_sc0000542.1_g000003
rosa_roxburghii	Rroxscaffold_6G00388710
rosa_rugosa	Rorug03G0287000
rosa_samantha	Rh3AG331400 Rh3CG364200 Rh3DG367500
rosa_wichuraiana	Rw3G029010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1277
AccI	GTMKAC	2 cut(s)	1282, 1587
AciI	CCGC	3 cut(s)	129, 974, 1178
AclWI	GGATC	2 cut(s)	1122, 1371
AcoI	YGGCCR	3 cut(s)	678, 1469, 1771
AcsI	RAATTY	3 cut(s)	536, 574, 996
AcuI	CTGAAG	2 cut(s)	533, 1005
AdeI	CACNNNGTG	1 cut(s)	1504
AfaI	GTAC	3 cut(s)	394, 1889, 1897
AfiI	CCNNNNNNNGG	3 cut(s)	348, 1251, 1277
AflII	CTTAAG	2 cut(s)	1631, 1642
AflIII	ACRYGT	1 cut(s)	1072
AjnI	CCWGG	5 cut(s)	347, 808, 1276, 1441, 1574
AluBI	AGCT	6 cut(s)	187, 414, 566, 1485, 1641, 1847
AluI	AGCT	6 cut(s)	187, 414, 566, 1485, 1641, 1847
Alw21I	GWGCWC	1 cut(s)	456
Alw26I	GTCTC	3 cut(s)	1420, 1618, 1957
Alw44I	GTGCAC	1 cut(s)	452
AlwI	GGATC	2 cut(s)	1122, 1371
AoxI	GGCC	9 cut(s)	194, 678, 731, 1242, 1253, 1259, 1469, 1654, 1771
ApaLI	GTGCAC	1 cut(s)	452
ApeKI	GCWGC	4 cut(s)	220, 518, 1131, 1553
ApoI	RAATTY	3 cut(s)	536, 574, 996
Asp700I	GAANNNNTTC	2 cut(s)	855, 1732
AspA2I	CCTAGG	1 cut(s)	1250
AspLEI	GCGC	1 cut(s)	122
AspS9I	GGNCC	5 cut(s)	194, 1081, 1160, 1247, 1975
AsuHPI	GGTGA	4 cut(s)	331, 829, 1802, 1835
AvaII	GGWCC	4 cut(s)	1081, 1160, 1247, 1975
AvrII	CCTAGG	1 cut(s)	1250
BaeGI	GKGCMC	2 cut(s)	456, 1805
BalI	TGGCCA	1 cut(s)	1471
BarI	GAAGNNNNNNTAC	2 cut(s)	1717, 1749
Bbv12I	GWGCWC	1 cut(s)	456
BbvI	GCAGC	4 cut(s)	232, 530, 1143, 1565
BccI	CCATC	6 cut(s)	635, 754, 1451, 1461, 1599, 1763
BcgI	CGANNNNNNTGC	2 cut(s)	75, 109
BciT130I	CCWGG	5 cut(s)	349, 810, 1278, 1443, 1576
BciVI	GTATCC	1 cut(s)	1029
BcoDI	GTCTC	3 cut(s)	1420, 1618, 1957
BfaI	CTAG	3 cut(s)	797, 1251, 1524
BfmI	CTRYAG	3 cut(s)	81, 209, 653
BfrI	CTTAAG	2 cut(s)	1631, 1642
BfuI	GTATCC	1 cut(s)	1029
BglII	AGATCT	1 cut(s)	317
BisI	GCNGC	4 cut(s)	221, 519, 1132, 1554
BlnI	CCTAGG	1 cut(s)	1250
BlsI	GCNGC	4 cut(s)	222, 520, 1133, 1555
BmcAI	AGTACT	2 cut(s)	394, 1897
Bme1390I	CCNGG	5 cut(s)	349, 810, 1278, 1443, 1576
Bme18I	GGWCC	4 cut(s)	1081, 1160, 1247, 1975
BmgT120I	GGNCC	5 cut(s)	194, 1081, 1160, 1247, 1975
BmiI	GGNNCC	4 cut(s)	195, 1083, 1344, 1977
BmrFI	CCNGG	5 cut(s)	349, 810, 1278, 1443, 1576
BmsI	GCATC	8 cut(s)	68, 82, 417, 473, 689, 969, 1362, 1387
BpmI	CTGGAG	1 cut(s)	1731
BsaAI	YACGTR	1 cut(s)	776
BsaJI	CCNNGG	5 cut(s)	615, 828, 1250, 1256, 1575
BsaXI	ACNNNNNCTCC	2 cut(s)	154, 184
Bsc4I	CCNNNNNNNGG	3 cut(s)	348, 1251, 1277
Bse118I	RCCGGY	1 cut(s)	1244
Bse1I	ACTGG	2 cut(s)	112, 1748
Bse3DI	GCAATG	2 cut(s)	277, 633
BseBI	CCWGG	5 cut(s)	349, 810, 1278, 1443, 1576
BseDI	CCNNGG	5 cut(s)	615, 828, 1250, 1256, 1575
BseLI	CCNNNNNNNGG	3 cut(s)	348, 1251, 1277
BseMI	GCAATG	2 cut(s)	277, 633
BseMII	CTCAG	4 cut(s)	1320, 1358, 1629, 1947
BseNI	ACTGG	2 cut(s)	112, 1748
BseSI	GKGCMC	2 cut(s)	456, 1805
BseXI	GCAGC	4 cut(s)	232, 530, 1143, 1565
BseYI	CCCAGC	1 cut(s)	1847
BsgI	GTGCAG	2 cut(s)	1150, 1640
BshFI	GGCC	9 cut(s)	196, 680, 733, 1244, 1255, 1261, 1471, 1656, 1773
BsiHKAI	GWGCWC	1 cut(s)	456
BsiSI	CCGG	1 cut(s)	1245
BslFI	GGGAC	4 cut(s)	239, 1067, 1509, 1592
BslI	CCNNNNNNNGG	3 cut(s)	348, 1251, 1277
BsmAI	GTCTC	3 cut(s)	1420, 1618, 1957
BsmFI	GGGAC	4 cut(s)	239, 1067, 1509, 1592
BsmI	GAATGC	2 cut(s)	131, 1721
BsnI	GGCC	9 cut(s)	196, 680, 733, 1244, 1255, 1261, 1471, 1656, 1773
Bsp1286I	GDGCHC	2 cut(s)	456, 1805
Bsp143I	GATC	5 cut(s)	317, 324, 331, 1114, 1363
BspACI	CCGC	3 cut(s)	129, 974, 1178
BspANI	GGCC	9 cut(s)	196, 680, 733, 1244, 1255, 1261, 1471, 1656, 1773
BspCNI	CTCAG	4 cut(s)	1321, 1359, 1628, 1948
BspHI	TCATGA	1 cut(s)	922
BspLI	GGNNCC	4 cut(s)	195, 1083, 1344, 1977
BspMAI	CTGCAG	2 cut(s)	213, 657
BspPI	GGATC	2 cut(s)	1122, 1371
BspTI	CTTAAG	2 cut(s)	1631, 1642
BsrDI	GCAATG	2 cut(s)	277, 633
BsrFI	RCCGGY	1 cut(s)	1244
BsrI	ACTGG	2 cut(s)	112, 1748
BssAI	RCCGGY	1 cut(s)	1244
BssECI	CCNNGG	5 cut(s)	615, 828, 1250, 1256, 1575
BssMI	GATC	5 cut(s)	317, 324, 331, 1114, 1363
BssT1I	CCWWGG	3 cut(s)	615, 828, 1250
Bst2UI	CCWGG	5 cut(s)	349, 810, 1278, 1443, 1576
Bst4CI	ACNGT	3 cut(s)	100, 1502, 1612
Bst6I	CTCTTC	5 cut(s)	488, 981, 1269, 1791, 1848
BstAFI	CTTAAG	2 cut(s)	1631, 1642
BstAPI	GCANNNNNTGC	1 cut(s)	217
BstBAI	YACGTR	1 cut(s)	776
BstC8I	GCNNGC	5 cut(s)	974, 1092, 1203, 1377, 1686
BstDEI	CTNAG	8 cut(s)	15, 695, 932, 1108, 1329, 1367, 1615, 1956
BstEII	GGTNACC	1 cut(s)	337
BstHHI	GCGC	1 cut(s)	122
BstKTI	GATC	5 cut(s)	320, 327, 334, 1117, 1366
BstMAI	GTCTC	3 cut(s)	1420, 1618, 1957
BstMBI	GATC	5 cut(s)	317, 324, 331, 1114, 1363
BstMWI	GCNNNNNNNGC	6 cut(s)	128, 193, 202, 217, 405, 716
BstNI	CCWGG	5 cut(s)	349, 810, 1278, 1443, 1576
BstPI	GGTNACC	1 cut(s)	337
BstSCI	CCNGG	5 cut(s)	347, 808, 1276, 1441, 1574
BstSFI	CTRYAG	3 cut(s)	81, 209, 653
BstSLI	GKGCMC	2 cut(s)	456, 1805
BstV1I	GCAGC	4 cut(s)	232, 530, 1143, 1565
BstX2I	RGATCY	2 cut(s)	317, 1363
BstXI	CCANNNNNNTGG	3 cut(s)	272, 749, 1111
BstYI	RGATCY	2 cut(s)	317, 1363
BsuI	GTATCC	1 cut(s)	1029
BsuRI	GGCC	9 cut(s)	196, 680, 733, 1244, 1255, 1261, 1471, 1656, 1773
BtsI	GCAGTG	1 cut(s)	1468
BtsIMutI	CAGTG	3 cut(s)	870, 1468, 1623
Cac8I	GCNNGC	5 cut(s)	974, 1092, 1203, 1377, 1686
CciI	TCATGA	1 cut(s)	922
CfoI	GCGC	1 cut(s)	122
Cfr10I	RCCGGY	1 cut(s)	1244
Cfr13I	GGNCC	5 cut(s)	194, 1081, 1160, 1247, 1975
Csp6I	GTAC	3 cut(s)	393, 1888, 1896
CviQI	GTAC	3 cut(s)	393, 1888, 1896
DdeI	CTNAG	8 cut(s)	15, 695, 932, 1108, 1329, 1367, 1615, 1956
DpnI	GATC	5 cut(s)	319, 326, 333, 1116, 1365
DpnII	GATC	5 cut(s)	317, 324, 331, 1114, 1363
DraI	TTTAAA	1 cut(s)	471
DraIII	CACNNNGTG	1 cut(s)	1504
EaeI	YGGCCR	3 cut(s)	678, 1469, 1771
Eam1104I	CTCTTC	5 cut(s)	488, 981, 1269, 1791, 1848
EarI	CTCTTC	5 cut(s)	488, 981, 1269, 1791, 1848
Eco130I	CCWWGG	3 cut(s)	615, 828, 1250
Eco147I	AGGCCT	2 cut(s)	1255, 1656
Eco47I	GGWCC	4 cut(s)	1081, 1160, 1247, 1975
Eco57I	CTGAAG	2 cut(s)	533, 1005
Eco91I	GGTNACC	1 cut(s)	337
EcoO109I	RGGNCCY	1 cut(s)	194
EcoO65I	GGTNACC	1 cut(s)	337
EcoRI	GAATTC	2 cut(s)	536, 996
EcoRII	CCWGG	5 cut(s)	347, 808, 1276, 1441, 1574
EcoT14I	CCWWGG	3 cut(s)	615, 828, 1250
ErhI	CCWWGG	3 cut(s)	615, 828, 1250
FalI	AAGNNNNNCTT	2 cut(s)	1625, 1657
FaqI	GGGAC	4 cut(s)	239, 1067, 1509, 1592
FauI	CCCGC	1 cut(s)	981
FblI	GTMKAC	2 cut(s)	1282, 1587
Fnu4HI	GCNGC	4 cut(s)	221, 519, 1132, 1554
Fsp4HI	GCNGC	4 cut(s)	221, 519, 1132, 1554
FspBI	CTAG	3 cut(s)	797, 1251, 1524
GlaI	GCGC	1 cut(s)	121
GluI	GCNGC	4 cut(s)	221, 519, 1132, 1554
GsaI	CCCAGC	1 cut(s)	1851
GsuI	CTGGAG	1 cut(s)	1731
HaeIII	GGCC	9 cut(s)	196, 680, 733, 1244, 1255, 1261, 1471, 1656, 1773
HapII	CCGG	1 cut(s)	1245
HhaI	GCGC	1 cut(s)	122
Hin6I	GCGC	1 cut(s)	120
HinP1I	GCGC	1 cut(s)	120
HincII	GTYRAC	2 cut(s)	788, 1070
HindII	GTYRAC	2 cut(s)	788, 1070
HindIII	AAGCTT	2 cut(s)	1483, 1639
HinfI	GANTC	5 cut(s)	496, 941, 1331, 1841, 1916
HpaII	CCGG	1 cut(s)	1245
HphI	GGTGA	4 cut(s)	331, 829, 1802, 1835
Hpy166II	GTNNAC	6 cut(s)	454, 788, 868, 1070, 1283, 1588
Hpy188I	TCNGA	7 cut(s)	513, 1054, 1150, 1336, 1368, 1885, 1957
Hpy188III	TCNNGA	9 cut(s)	377, 386, 424, 860, 923, 1403, 1524, 1712, 1901
Hpy8I	GTNNAC	6 cut(s)	454, 788, 868, 1070, 1283, 1588
HpyAV	CCTTC	9 cut(s)	508, 552, 674, 721, 885, 1184, 1234, 1301, 1646
HpyCH4III	ACNGT	3 cut(s)	100, 1502, 1612
HpyCH4IV	ACGT	2 cut(s)	775, 1072
HpyF10VI	GCNNNNNNNGC	6 cut(s)	128, 193, 202, 217, 405, 716
HpyF3I	CTNAG	8 cut(s)	15, 695, 932, 1108, 1329, 1367, 1615, 1956
HpySE526I	ACGT	2 cut(s)	775, 1072
HspAI	GCGC	1 cut(s)	120
Kzo9I	GATC	5 cut(s)	317, 324, 331, 1114, 1363
LmnI	GCTCC	1 cut(s)	1750
Lsp1109I	GCAGC	4 cut(s)	232, 530, 1143, 1565
LweI	GCATC	8 cut(s)	68, 82, 417, 473, 689, 969, 1362, 1387
MaeI	CTAG	3 cut(s)	797, 1251, 1524
MaeII	ACGT	2 cut(s)	775, 1072
MaeIII	GTNAC	3 cut(s)	337, 771, 1823
MalI	GATC	5 cut(s)	319, 326, 333, 1116, 1365
MboI	GATC	5 cut(s)	317, 324, 331, 1114, 1363
MflI	RGATCY	2 cut(s)	317, 1363
MhlI	GDGCHC	2 cut(s)	456, 1805
MlsI	TGGCCA	1 cut(s)	1471
MluNI	TGGCCA	1 cut(s)	1471
MlyI	GAGTC	2 cut(s)	505, 1340
MmeI	TCCRAC	2 cut(s)	973, 1301
Mox20I	TGGCCA	1 cut(s)	1471
MroXI	GAANNNNTTC	2 cut(s)	855, 1732
MscI	TGGCCA	1 cut(s)	1471
MseI	TTAA	6 cut(s)	470, 906, 1224, 1449, 1632, 1643
MslI	CAYNNNNRTG	1 cut(s)	843
Msp20I	TGGCCA	1 cut(s)	1471
MspA1I	CMGCKG	2 cut(s)	976, 1847
MspCI	CTTAAG	2 cut(s)	1631, 1642
MspI	CCGG	1 cut(s)	1245
MspR9I	CCNGG	5 cut(s)	349, 810, 1278, 1443, 1576
Mva1269I	GAATGC	2 cut(s)	131, 1721
MvaI	CCWGG	5 cut(s)	349, 810, 1278, 1443, 1576
MwoI	GCNNNNNNNGC	6 cut(s)	128, 193, 202, 217, 405, 716
NdeII	GATC	5 cut(s)	317, 324, 331, 1114, 1363
NlaIV	GGNNCC	4 cut(s)	195, 1083, 1344, 1977
NmeAIII	GCCGAG	2 cut(s)	706, 1237
NmuCI	GTSAC	2 cut(s)	337, 1823
PagI	TCATGA	1 cut(s)	922
PceI	AGGCCT	2 cut(s)	1255, 1656
PctI	GAATGC	2 cut(s)	131, 1721
PdmI	GAANNNNTTC	2 cut(s)	855, 1732
PfeI	GAWTC	3 cut(s)	941, 1841, 1916
PflFI	GACNNNGTC	2 cut(s)	482, 1583
PflMI	CCANNNNNTGG	1 cut(s)	1277
PkrI	GCNGC	4 cut(s)	222, 520, 1133, 1555
PleI	GAGTC	2 cut(s)	504, 1339
PpsI	GAGTC	2 cut(s)	504, 1339
Ppu21I	YACGTR	1 cut(s)	776
Psp6I	CCWGG	5 cut(s)	347, 808, 1276, 1441, 1574
PspEI	GGTNACC	1 cut(s)	337
PspFI	CCCAGC	1 cut(s)	1847
PspGI	CCWGG	5 cut(s)	347, 808, 1276, 1441, 1574
PspN4I	GGNNCC	4 cut(s)	195, 1083, 1344, 1977
PspPI	GGNCC	5 cut(s)	194, 1081, 1160, 1247, 1975
PsrI	GAACNNNNNNTAC	2 cut(s)	1927, 1959
PstI	CTGCAG	2 cut(s)	213, 657
PsuI	RGATCY	2 cut(s)	317, 1363
PsyI	GACNNNGTC	2 cut(s)	482, 1583
PvuII	CAGCTG	1 cut(s)	1847
RsaI	GTAC	3 cut(s)	394, 1889, 1897
RsaNI	GTAC	3 cut(s)	393, 1888, 1896
RseI	CAYNNNNRTG	1 cut(s)	843
SaqAI	TTAA	6 cut(s)	470, 906, 1224, 1449, 1632, 1643
SatI	GCNGC	4 cut(s)	221, 519, 1132, 1554
Sau3AI	GATC	5 cut(s)	317, 324, 331, 1114, 1363
Sau96I	GGNCC	5 cut(s)	194, 1081, 1160, 1247, 1975
ScaI	AGTACT	2 cut(s)	394, 1897
SchI	GAGTC	2 cut(s)	505, 1340
ScrFI	CCNGG	5 cut(s)	349, 810, 1278, 1443, 1576
SduI	GDGCHC	2 cut(s)	456, 1805
SfaNI	GCATC	8 cut(s)	68, 82, 417, 473, 689, 969, 1362, 1387
SfcI	CTRYAG	3 cut(s)	81, 209, 653
SinI	GGWCC	4 cut(s)	1081, 1160, 1247, 1975
SmiMI	CAYNNNNRTG	1 cut(s)	843
SmlI	CTYRAG	2 cut(s)	1631, 1642
SmoI	CTYRAG	2 cut(s)	1631, 1642
SseBI	AGGCCT	2 cut(s)	1255, 1656
SsiI	CCGC	3 cut(s)	129, 974, 1178
SspI	AATATT	1 cut(s)	294
SspMI	CTAG	3 cut(s)	797, 1251, 1524
StuI	AGGCCT	2 cut(s)	1255, 1656
StyD4I	CCNGG	5 cut(s)	347, 808, 1276, 1441, 1574
StyI	CCWWGG	3 cut(s)	615, 828, 1250
TaaI	ACNGT	3 cut(s)	100, 1502, 1612
TaiI	ACGT	2 cut(s)	778, 1075
TaqI	TCGA	4 cut(s)	95, 102, 889, 1679
TatI	WGTACW	2 cut(s)	392, 1895
TfiI	GAWTC	3 cut(s)	941, 1841, 1916
Tru1I	TTAA	6 cut(s)	470, 906, 1224, 1449, 1632, 1643
Tru9I	TTAA	6 cut(s)	470, 906, 1224, 1449, 1632, 1643
TscAI	CASTG	3 cut(s)	877, 1468, 1623
TseFI	GTSAC	2 cut(s)	337, 1823
TseI	GCWGC	4 cut(s)	220, 518, 1131, 1553
Tsp45I	GTSAC	2 cut(s)	337, 1823
TspDTI	ATGAA	9 cut(s)	268, 549, 911, 1314, 1684, 1742, 1829, 1890, 1920
TspGWI	ACGGA	3 cut(s)	1355, 1660, 1905
TspRI	CASTG	3 cut(s)	877, 1468, 1623
Tth111I	GACNNNGTC	2 cut(s)	482, 1583
Van91I	CCANNNNNTGG	1 cut(s)	1277
Vha464I	CTTAAG	2 cut(s)	1631, 1642
VneI	GTGCAC	1 cut(s)	452
VpaK11BI	GGWCC	4 cut(s)	1081, 1160, 1247, 1975
XapI	RAATTY	3 cut(s)	536, 574, 996
XbaI	TCTAGA	1 cut(s)	1523
XmaJI	CCTAGG	1 cut(s)	1250
XmiI	GTMKAC	2 cut(s)	1282, 1587
XmnI	GAANNNNTTC	2 cut(s)	855, 1732
XspI	CTAG	3 cut(s)	797, 1251, 1524
ZrmI	AGTACT	2 cut(s)	394, 1897

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.