MD12G1089800.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0028718
Physical Location & Seq
Forward (+)
11249506 .. 11250421
916 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1089800.v1.1.491

Sequence Viewer

Length: 588 bp
ATGTCAAATGAATCCAACGTTTTCCTACATCAGGTTATGTTGCTTGAGGGTGACGTTATGTATGCAGGAATGGTTCATTGTGTGACAATTCAAATGGGTTTTGGAATGGATGTGTATTTTGGTAATACAATGATTTATTTTTATGTGAAATGCGGAGGACATGGTTATTCTCATAAGTTGTTTGATGAAATGTCTCAAAGAGATTTGCTTTCTTGGACATCGACGATTTCCGGTTATGTTTTGAAGGGAATGTTGCCTGGCAAAGTGGCGCCGGGAATTGAGACATTAACTTTGGTTATATCAGCAGTGACCAAAGATGGTATTCTTTCCCAAGGTGAAAGCTTGAATTGTGTGGCGATAAAGCGTGGGTTACGTGATCACGTTTTGCAGAAGTCTTTGTTGGACCTCTATGCCAAGCGTGGAGAATTAGGAAACTCATATTGGCTGTTCAGAGCAATCCCTCGCGGGAACGGCATTACTTGGGGTGCAATGATGTTTGGCTTCATTCAAAGTGGTTGGTTTAGTGAAGCTGTTGGACTGTTTCGTGAAATGCAAGATTCGGTCCGGAACCAAGGGCTGAGATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

196

Amino Acids

21.82

Weight (kDa)

7.74

Isoelectric Point (pI)

27.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 159 - 186 5.3e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028718)

Species Orthologous Gene IDs
malus_domestica MD12G1089800.v1.1
pyrus_communis pycom12g07570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 268
AccII CGCG 1 cut(s) 465
AccIII TCCGGA 1 cut(s) 564
AciI CCGC 2 cut(s) 153, 465
AclI AACGTT 1 cut(s) 18
AcyI GRCGYC 1 cut(s) 269
AfiI CCNNNNNNNGG 1 cut(s) 31
AgsI TTSAA 4 cut(s) 92, 244, 346, 509
AjnI CCWGG 1 cut(s) 256
AjuI GAANNNNNNNTTGG 4 cut(s) 383, 415, 424, 456
AluBI AGCT 2 cut(s) 342, 530
AluI AGCT 2 cut(s) 342, 530
Alw26I GTCTC 2 cut(s) 198, 275
Aor13HI TCCGGA 1 cut(s) 564
ArsI GACNNNNNNTTYG 2 cut(s) 274, 306
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 2 cut(s) 403, 562
AsuC2I CCSGG 1 cut(s) 273
AsuHPI GGTGA 2 cut(s) 62, 347
AvaII GGWCC 2 cut(s) 403, 562
BanI GGYRCC 1 cut(s) 268
BccI CCATC 1 cut(s) 311
BceAI ACGGC 1 cut(s) 487
BciT130I CCWGG 1 cut(s) 258
BclI TGATCA 1 cut(s) 376
BcnI CCSGG 1 cut(s) 273
BcoDI GTCTC 2 cut(s) 198, 275
BfoI RGCGCY 1 cut(s) 272
Bme1390I CCNGG 2 cut(s) 258, 273
Bme18I GGWCC 2 cut(s) 403, 562
BmgT120I GGNCC 2 cut(s) 403, 562
BmiI GGNNCC 2 cut(s) 270, 569
BmrFI CCNGG 2 cut(s) 258, 273
BpuEI CTTGAG 1 cut(s) 65
BpuMI CCSGG 1 cut(s) 273
BsaAI YACGTR 1 cut(s) 374
BsaHI GRCGYC 1 cut(s) 269
BsaJI CCNNGG 2 cut(s) 331, 571
BsaWI WCCGGW 2 cut(s) 230, 564
Bsc4I CCNNNNNNNGG 1 cut(s) 31
Bse3DI GCAATG 1 cut(s) 495
BseAI TCCGGA 1 cut(s) 564
BseBI CCWGG 1 cut(s) 258
BseDI CCNNGG 2 cut(s) 331, 571
BseGI GGATG 1 cut(s) 115
BseLI CCNNNNNNNGG 1 cut(s) 31
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 1 cut(s) 569
Bsh1236I CGCG 1 cut(s) 465
BshNI GGYRCC 1 cut(s) 268
BsiSI CCGG 3 cut(s) 231, 272, 565
BslI CCNNNNNNNGG 1 cut(s) 31
BsmAI GTCTC 2 cut(s) 198, 275
Bsp13I TCCGGA 1 cut(s) 564
Bsp143I GATC 1 cut(s) 376
BspACI CCGC 2 cut(s) 153, 465
BspCNI CTCAG 1 cut(s) 570
BspEI TCCGGA 1 cut(s) 564
BspFNI CGCG 1 cut(s) 465
BspLI GGNNCC 2 cut(s) 270, 569
BspT107I GGYRCC 1 cut(s) 268
BsrDI GCAATG 1 cut(s) 495
BssECI CCNNGG 2 cut(s) 331, 571
BssMI GATC 1 cut(s) 376
BssNI GRCGYC 1 cut(s) 269
BssT1I CCWWGG 2 cut(s) 331, 571
Bst2UI CCWGG 1 cut(s) 258
Bst4CI ACNGT 1 cut(s) 540
BstACI GRCGYC 1 cut(s) 269
BstBAI YACGTR 1 cut(s) 374
BstDEI CTNAG 1 cut(s) 578
BstENI CCTNNNNNAGG 1 cut(s) 29
BstF5I GGATG 1 cut(s) 115
BstFNI CGCG 1 cut(s) 465
BstH2I RGCGCY 1 cut(s) 272
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 1 cut(s) 379
BstMAI GTCTC 2 cut(s) 198, 275
BstMBI GATC 1 cut(s) 376
BstMWI GCNNNNNNNGC 1 cut(s) 471
BstNI CCWGG 1 cut(s) 258
BstSCI CCNGG 2 cut(s) 256, 271
BstUI CGCG 1 cut(s) 465
BtsCI GGATG 1 cut(s) 115
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 1 cut(s) 312
CfoI GCGC 1 cut(s) 271
Cfr13I GGNCC 2 cut(s) 403, 562
CpoI CGGWCCG 1 cut(s) 562
CspI CGGWCCG 1 cut(s) 562
CviAII CATG 1 cut(s) 161
CviJI RGCY 5 cut(s) 342, 445, 501, 530, 577
CviKI_1 RGCY 5 cut(s) 342, 445, 501, 530, 577
DdeI CTNAG 1 cut(s) 578
DinI GGCGCC 1 cut(s) 270
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
Eco130I CCWWGG 2 cut(s) 331, 571
Eco47I GGWCC 2 cut(s) 403, 562
EcoNI CCTNNNNNAGG 1 cut(s) 29
EcoRII CCWGG 1 cut(s) 256
EcoT14I CCWWGG 2 cut(s) 331, 571
EgeI GGCGCC 1 cut(s) 270
EheI GGCGCC 1 cut(s) 270
ErhI CCWWGG 2 cut(s) 331, 571
FaeI CATG 1 cut(s) 164
FatI CATG 1 cut(s) 160
FauI CCCGC 1 cut(s) 458
FbaI TGATCA 1 cut(s) 376
FokI GGATG 1 cut(s) 122
GlaI GCGC 1 cut(s) 270
HaeII RGCGCY 1 cut(s) 272
HapII CCGG 3 cut(s) 231, 272, 565
HhaI GCGC 1 cut(s) 271
Hin1I GRCGYC 1 cut(s) 269
Hin1II CATG 1 cut(s) 164
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HindIII AAGCTT 1 cut(s) 340
HinfI GANTC 2 cut(s) 11, 557
HpaII CCGG 3 cut(s) 231, 272, 565
HphI GGTGA 2 cut(s) 62, 347
Hpy188I TCNGA 1 cut(s) 452
Hpy188III TCNNGA 2 cut(s) 545, 565
Hpy99I CGWCG 1 cut(s) 226
HpyAV CCTTC 1 cut(s) 238
HpyCH4III ACNGT 1 cut(s) 540
HpyCH4IV ACGT 4 cut(s) 18, 54, 373, 381
HpyCH4V TGCA 4 cut(s) 65, 388, 488, 553
HpyF10VI GCNNNNNNNGC 1 cut(s) 471
HpyF3I CTNAG 1 cut(s) 578
HpySE526I ACGT 4 cut(s) 18, 54, 373, 381
Hsp92I GRCGYC 1 cut(s) 269
Hsp92II CATG 1 cut(s) 164
HspAI GCGC 1 cut(s) 269
KasI GGCGCC 1 cut(s) 268
Kpn2I TCCGGA 1 cut(s) 564
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 1 cut(s) 376
LpnPI CCDG 7 cut(s) 17, 51, 243, 244, 270, 285, 578
MaeII ACGT 4 cut(s) 18, 54, 373, 381
MaeIII GTNAC 4 cut(s) 50, 82, 307, 369
MalI GATC 1 cut(s) 378
MboI GATC 1 cut(s) 376
MluCI AATT 4 cut(s) 87, 276, 346, 425
Mly113I GGCGCC 1 cut(s) 269
MmeI TCCRAC 3 cut(s) 39, 381, 514
MnlI CCTC 4 cut(s) 40, 149, 416, 471
MroI TCCGGA 1 cut(s) 564
MseI TTAA 1 cut(s) 287
MspI CCGG 3 cut(s) 231, 272, 565
MspR9I CCNGG 2 cut(s) 258, 273
MvaI CCWGG 1 cut(s) 258
MvnI CGCG 1 cut(s) 465
MwoI GCNNNNNNNGC 1 cut(s) 471
NarI GGCGCC 1 cut(s) 269
NciI CCSGG 1 cut(s) 273
NdeII GATC 1 cut(s) 376
NlaIII CATG 1 cut(s) 164
NlaIV GGNNCC 2 cut(s) 270, 569
NmuCI GTSAC 3 cut(s) 50, 82, 307
PfeI GAWTC 2 cut(s) 11, 557
PluTI GGCGCC 1 cut(s) 272
Ppu21I YACGTR 1 cut(s) 374
Psp1406I AACGTT 1 cut(s) 18
Psp6I CCWGG 1 cut(s) 256
PspGI CCWGG 1 cut(s) 256
PspN4I GGNNCC 2 cut(s) 270, 569
PspPI GGNCC 2 cut(s) 403, 562
Rsr2I CGGWCCG 1 cut(s) 562
RsrII CGGWCCG 1 cut(s) 562
SaqAI TTAA 1 cut(s) 287
Sau3AI GATC 1 cut(s) 376
Sau96I GGNCC 2 cut(s) 403, 562
ScrFI CCNGG 2 cut(s) 258, 273
SetI ASST 9 cut(s) 21, 36, 57, 337, 344, 376, 384, 408, 532
SfoI GGCGCC 1 cut(s) 270
SinI GGWCC 2 cut(s) 403, 562
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 4 cut(s) 87, 276, 346, 425
SsiI CCGC 2 cut(s) 153, 465
SspDI GGCGCC 1 cut(s) 268
StyD4I CCNGG 2 cut(s) 256, 271
StyI CCWWGG 2 cut(s) 331, 571
TaaI ACNGT 1 cut(s) 540
TaiI ACGT 4 cut(s) 21, 57, 376, 384
TaqI TCGA 1 cut(s) 221
TaqII GACCGA 1 cut(s) 550
TasI AATT 4 cut(s) 87, 276, 346, 425
TfiI GAWTC 2 cut(s) 11, 557
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TscAI CASTG 1 cut(s) 312
TseFI GTSAC 3 cut(s) 50, 82, 307
Tsp45I GTSAC 3 cut(s) 50, 82, 307
TspDTI ATGAA 4 cut(s) 24, 65, 201, 493
TspRI CASTG 1 cut(s) 312
VpaK11BI GGWCC 2 cut(s) 403, 562
XagI CCTNNNNNAGG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.