MD12G1091800.v1.1

Belongs to the pyrroline-5-carboxylate reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0024418
Physical Location & Seq
Reverse (-)
11567563 .. 11572123
4561 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1091800.v1.1.491

Sequence Viewer

Length: 843 bp
ATGGCAGGAGGTGGGGAAGTGGAAGTCAAACCAATTGACAAGGAAGCCTTCAATTTGGGGTTCATTGGAGCTGGTAACTTGGCAGCAAGTGTGGCCATCGGTTTGGTCAACCAGGGGATTCTACCACCTTCTAGAATTTTCACGGCTCACCGGAATCCAGAAAGCCGGCTGGATTTTACATCTGTTGGAATTCAGGTTTATCAGAACAATCATCAGGTAGCTGAAGAGTGTGATGTAATCATTTTCTCAGTGAAACCTCAAATACTTAAGGAAGTAATCCTGGACTTACGGCCGCTACTTTCACAAAATAAACTTCTTGTGTCGCTTGCTGTGGGACACAAATTGAAAGCTCTACAGGAATGGGCCGGTCATTGTCGATTTGTTAGAGTAATCCCTAGTACCGCTTCTGTCATCGGTGAAGCAGCCACAGTTATGCACTTGGGTGAAGCTGCAACAGTAAACGACAGTGAGTTAATAGCAGACCTATTTGGAGCTGTTGGCAAGGTGTGGAAAATGGACGAGAAATTGTCTGATGCTGCTGCTGCTATTGGTGGTGCTGGTACAGCATTTGCGTATGTAGCAATAGAAGCTATGGCTGATGGAGGTGTAGCTGCAGGCTTACCACGAAAAATTTCTCTGAGTCTTGCTTCTCAAGCTTTCTATGGAGCAGCAGCCATGGTGATGAAAACAGGAAAACATCCAGGTCAGCTGAAAGACCAAGTTGCTTCACCTGGAGGAACCACCATTGCCGGGATTCATGAGCTTGAGAAGGGCGCATTTCGCGCAAGCATGATGAATGCGGTTGTTGCTACAACAAAGCGTAGCCATGAGATGGGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 ko01110 ko01130 ko01230 map00330 map01100 map01110 map01130 map01230
Pfam Domains
Protein Families
F420_oxidored P5CR_dimer

Protein Analysis

281

Amino Acids

29.31

Weight (kDa)

6.71

Isoelectric Point (pI)

29.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F420_oxidored PF03807 19 - 112 2.4e-16 NADP oxidoreductase coenzyme F420-dependent
P5CR_dimer PF14748 174 - 278 1.4e-34 Pyrroline-5-carboxylate reductase dimerisation
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024418)

Species Orthologous Gene IDs
malus_domestica MD12G1091800.v1.1
prunus_persica Prupe.7G045400_v2.0.a1
pyrus_communis pycom12g07750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 832
AccII CGCG 1 cut(s) 783
AciI CCGC 3 cut(s) 293, 402, 800
AcoI YGGCCR 2 cut(s) 93, 290
AcsI RAATTY 3 cut(s) 135, 189, 630
AcuI CTGAAG 1 cut(s) 243
AfaI GTAC 2 cut(s) 400, 562
AfiI CCNNNNNNNGG 2 cut(s) 750, 832
AflII CTTAAG 1 cut(s) 266
AgsI TTSAA 2 cut(s) 52, 346
AjnI CCWGG 4 cut(s) 111, 279, 700, 730
AleI CACNNNNGTG 1 cut(s) 441
AoxI GGCC 3 cut(s) 93, 290, 363
ApeKI GCWGC 9 cut(s) 83, 422, 449, 536, 539, 542, 611, 668, 671
ApoI RAATTY 3 cut(s) 135, 189, 630
Asp700I GAANNNNTTC 1 cut(s) 631
AspLEI GCGC 2 cut(s) 776, 785
AspS9I GGNCC 1 cut(s) 363
AsuC2I CCSGG 1 cut(s) 751
AsuHPI GGTGA 5 cut(s) 140, 428, 455, 691, 720
BalI TGGCCA 1 cut(s) 95
BbvI GCAGC 9 cut(s) 95, 434, 436, 523, 526, 529, 598, 680, 683
BccI CCATC 3 cut(s) 104, 593, 826
BceAI ACGGC 2 cut(s) 159, 305
BciT130I CCWGG 4 cut(s) 113, 281, 702, 732
BcnI CCSGG 1 cut(s) 751
BfaI CTAG 2 cut(s) 132, 396
BfmI CTRYAG 2 cut(s) 353, 612
BfrI CTTAAG 1 cut(s) 266
Bme1390I CCNGG 5 cut(s) 113, 281, 702, 732, 751
BmgT120I GGNCC 1 cut(s) 363
BmiI GGNNCC 1 cut(s) 739
BmrFI CCNGG 5 cut(s) 113, 281, 702, 732, 751
BmsI GCATC 1 cut(s) 523
BpmI CTGGAG 1 cut(s) 753
BpuEI CTTGAG 2 cut(s) 636, 785
BpuMI CCSGG 1 cut(s) 751
BsaJI CCNNGG 2 cut(s) 112, 675
BsaWI WCCGGW 1 cut(s) 150
Bsc4I CCNNNNNNNGG 2 cut(s) 750, 832
Bse118I RCCGGY 2 cut(s) 165, 365
Bse3DI GCAATG 1 cut(s) 744
BseBI CCWGG 4 cut(s) 113, 281, 702, 732
BseDI CCNNGG 2 cut(s) 112, 675
BseGI GGATG 1 cut(s) 697
BseLI CCNNNNNNNGG 2 cut(s) 750, 832
BseMI GCAATG 1 cut(s) 744
BseMII CTCAG 2 cut(s) 261, 629
BseX3I CGGCCG 1 cut(s) 290
BseXI GCAGC 9 cut(s) 95, 434, 436, 523, 526, 529, 598, 680, 683
Bsh1236I CGCG 1 cut(s) 783
Bsh1285I CGRYCG 1 cut(s) 293
BshFI GGCC 3 cut(s) 95, 292, 365
BsiEI CGRYCG 1 cut(s) 293
BsiSI CCGG 4 cut(s) 151, 166, 366, 750
BslFI GGGAC 1 cut(s) 348
BslI CCNNNNNNNGG 2 cut(s) 750, 832
BsmFI GGGAC 1 cut(s) 348
BsmI GAATGC 1 cut(s) 802
BsnI GGCC 3 cut(s) 95, 292, 365
Bsp19I CCATGG 1 cut(s) 675
BspACI CCGC 3 cut(s) 293, 402, 800
BspANI GGCC 3 cut(s) 95, 292, 365
BspCNI CTCAG 2 cut(s) 260, 630
BspFNI CGCG 1 cut(s) 783
BspHI TCATGA 1 cut(s) 757
BspLI GGNNCC 1 cut(s) 739
BspMAI CTGCAG 1 cut(s) 616
BspTI CTTAAG 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 744
BsrFI RCCGGY 2 cut(s) 165, 365
BssAI RCCGGY 2 cut(s) 165, 365
BssECI CCNNGG 2 cut(s) 112, 675
BssT1I CCWWGG 1 cut(s) 675
Bst2UI CCWGG 4 cut(s) 113, 281, 702, 732
Bst4CI ACNGT 3 cut(s) 430, 457, 467
Bst6I CTCTTC 1 cut(s) 219
BstAFI CTTAAG 1 cut(s) 266
BstC8I GCNNGC 4 cut(s) 167, 327, 616, 787
BstDEI CTNAG 2 cut(s) 247, 638
BstDSI CCRYGG 1 cut(s) 675
BstF5I GGATG 1 cut(s) 697
BstFNI CGCG 1 cut(s) 783
BstHHI GCGC 2 cut(s) 776, 785
BstMCI CGRYCG 1 cut(s) 293
BstMWI GCNNNNNNNGC 9 cut(s) 92, 542, 563, 578, 587, 653, 780, 782, 806
BstNI CCWGG 4 cut(s) 113, 281, 702, 732
BstSCI CCNGG 5 cut(s) 111, 279, 700, 730, 749
BstSFI CTRYAG 2 cut(s) 353, 612
BstUI CGCG 1 cut(s) 783
BstV1I GCAGC 9 cut(s) 95, 434, 436, 523, 526, 529, 598, 680, 683
BstXI CCANNNNNNTGG 1 cut(s) 103
BstZI CGGCCG 1 cut(s) 290
BsuRI GGCC 3 cut(s) 95, 292, 365
BtgI CCRYGG 1 cut(s) 675
BtsCI GGATG 1 cut(s) 697
BtsIMutI CAGTG 2 cut(s) 255, 472
Cac8I GCNNGC 4 cut(s) 167, 327, 616, 787
CciI TCATGA 1 cut(s) 757
CfoI GCGC 2 cut(s) 776, 785
Cfr10I RCCGGY 2 cut(s) 165, 365
Cfr13I GGNCC 1 cut(s) 363
Csp6I GTAC 2 cut(s) 399, 561
CviAII CATG 4 cut(s) 676, 758, 790, 827
CviQI GTAC 2 cut(s) 399, 561
DdeI CTNAG 2 cut(s) 247, 638
EaeI YGGCCR 2 cut(s) 93, 290
EagI CGGCCG 1 cut(s) 290
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
EclXI CGGCCG 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 675
Eco52I CGGCCG 1 cut(s) 290
Eco57I CTGAAG 1 cut(s) 243
EcoRI GAATTC 1 cut(s) 189
EcoRII CCWGG 4 cut(s) 111, 279, 700, 730
EcoT14I CCWWGG 1 cut(s) 675
ErhI CCWWGG 1 cut(s) 675
FaeI CATG 4 cut(s) 679, 761, 793, 830
FaiI YATR 8 cut(s) 434, 576, 593, 663, 677, 759, 791, 828
FalI AAGNNNNNCTT 2 cut(s) 32, 64
FaqI GGGAC 1 cut(s) 348
FatI CATG 4 cut(s) 675, 757, 789, 826
FokI GGATG 1 cut(s) 684
FspBI CTAG 2 cut(s) 132, 396
GlaI GCGC 2 cut(s) 775, 784
GsuI CTGGAG 1 cut(s) 753
HaeIII GGCC 3 cut(s) 95, 292, 365
HapII CCGG 4 cut(s) 151, 166, 366, 750
HhaI GCGC 2 cut(s) 776, 785
Hin1II CATG 4 cut(s) 679, 761, 793, 830
Hin6I GCGC 2 cut(s) 774, 783
HinP1I GCGC 2 cut(s) 774, 783
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HindIII AAGCTT 1 cut(s) 654
HinfI GANTC 4 cut(s) 118, 154, 640, 754
HpaII CCGG 4 cut(s) 151, 166, 366, 750
HphI GGTGA 5 cut(s) 140, 428, 455, 691, 720
Hpy166II GTNNAC 2 cut(s) 109, 460
Hpy188I TCNGA 3 cut(s) 204, 532, 639
Hpy188III TCNNGA 3 cut(s) 132, 158, 758
Hpy8I GTNNAC 2 cut(s) 109, 460
HpyAV CCTTC 3 cut(s) 58, 138, 763
HpyCH4III ACNGT 3 cut(s) 430, 457, 467
HpyCH4V TGCA 3 cut(s) 436, 452, 614
HpyF10VI GCNNNNNNNGC 9 cut(s) 92, 542, 563, 578, 587, 653, 780, 782, 806
HpyF3I CTNAG 2 cut(s) 247, 638
Hsp92II CATG 4 cut(s) 679, 761, 793, 830
HspAI GCGC 2 cut(s) 774, 783
KroI GCCGGC 1 cut(s) 165
KroNI GCCGGC 1 cut(s) 167
LmnI GCTCC 3 cut(s) 68, 491, 665
Lsp1109I GCAGC 9 cut(s) 95, 434, 436, 523, 526, 529, 598, 680, 683
LweI GCATC 1 cut(s) 523
MaeI CTAG 2 cut(s) 132, 396
MaeIII GTNAC 1 cut(s) 74
MboII GAAGA 1 cut(s) 236
MfeI CAATTG 1 cut(s) 33
MlsI TGGCCA 1 cut(s) 95
MluCI AATT 7 cut(s) 33, 52, 135, 189, 341, 524, 630
MluNI TGGCCA 1 cut(s) 95
MlyI GAGTC 1 cut(s) 649
MmeI TCCRAC 1 cut(s) 166
MnlI CCTC 3 cut(s) 267, 596, 728
Mox20I TGGCCA 1 cut(s) 95
MroNI GCCGGC 1 cut(s) 165
MroXI GAANNNNTTC 1 cut(s) 631
MscI TGGCCA 1 cut(s) 95
MseI TTAA 2 cut(s) 267, 473
MslI CAYNNNNRTG 3 cut(s) 431, 441, 680
Msp20I TGGCCA 1 cut(s) 95
MspA1I CMGCKG 1 cut(s) 709
MspCI CTTAAG 1 cut(s) 266
MspI CCGG 4 cut(s) 151, 166, 366, 750
MspR9I CCNGG 5 cut(s) 113, 281, 702, 732, 751
MunI CAATTG 1 cut(s) 33
Mva1269I GAATGC 1 cut(s) 802
MvaI CCWGG 4 cut(s) 113, 281, 702, 732
MvnI CGCG 1 cut(s) 783
MwoI GCNNNNNNNGC 9 cut(s) 92, 542, 563, 578, 587, 653, 780, 782, 806
NaeI GCCGGC 1 cut(s) 167
NciI CCSGG 1 cut(s) 751
NcoI CCATGG 1 cut(s) 675
NgoMIV GCCGGC 1 cut(s) 165
NlaIII CATG 4 cut(s) 679, 761, 793, 830
NlaIV GGNNCC 1 cut(s) 739
OliI CACNNNNGTG 1 cut(s) 441
PagI TCATGA 1 cut(s) 757
PctI GAATGC 1 cut(s) 802
PdiI GCCGGC 1 cut(s) 167
PdmI GAANNNNTTC 1 cut(s) 631
PfeI GAWTC 3 cut(s) 118, 154, 754
PflMI CCANNNNNTGG 1 cut(s) 832
PfoI TCCNGGA 1 cut(s) 279
PleI GAGTC 1 cut(s) 648
PpsI GAGTC 1 cut(s) 648
Psp6I CCWGG 4 cut(s) 111, 279, 700, 730
PspGI CCWGG 4 cut(s) 111, 279, 700, 730
PspN4I GGNNCC 1 cut(s) 739
PspPI GGNCC 1 cut(s) 363
PstI CTGCAG 1 cut(s) 616
PvuII CAGCTG 1 cut(s) 709
RsaI GTAC 2 cut(s) 400, 562
RsaNI GTAC 2 cut(s) 399, 561
RseI CAYNNNNRTG 3 cut(s) 431, 441, 680
SaqAI TTAA 2 cut(s) 267, 473
Sau96I GGNCC 1 cut(s) 363
SchI GAGTC 1 cut(s) 649
ScrFI CCNGG 5 cut(s) 113, 281, 702, 732, 751
SfaNI GCATC 1 cut(s) 523
SfcI CTRYAG 2 cut(s) 353, 612
SmiMI CAYNNNNRTG 3 cut(s) 431, 441, 680
SmlI CTYRAG 3 cut(s) 266, 651, 764
SmoI CTYRAG 3 cut(s) 266, 651, 764
Sse9I AATT 7 cut(s) 33, 52, 135, 189, 341, 524, 630
SsiI CCGC 3 cut(s) 293, 402, 800
SspMI CTAG 2 cut(s) 132, 396
StyD4I CCNGG 5 cut(s) 111, 279, 700, 730, 749
StyI CCWWGG 1 cut(s) 675
TaaI ACNGT 3 cut(s) 430, 457, 467
TaqI TCGA 1 cut(s) 376
TasI AATT 7 cut(s) 33, 52, 135, 189, 341, 524, 630
TauI GCSGC 1 cut(s) 295
TfiI GAWTC 3 cut(s) 118, 154, 754
Tru1I TTAA 2 cut(s) 267, 473
Tru9I TTAA 2 cut(s) 267, 473
TscAI CASTG 2 cut(s) 255, 472
TseI GCWGC 9 cut(s) 83, 422, 449, 536, 539, 542, 611, 668, 671
TspDTI ATGAA 4 cut(s) 52, 698, 746, 809
TspRI CASTG 2 cut(s) 255, 472
Van91I CCANNNNNTGG 1 cut(s) 832
Vha464I CTTAAG 1 cut(s) 266
XapI RAATTY 3 cut(s) 135, 189, 630
XbaI TCTAGA 1 cut(s) 131
XmnI GAANNNNTTC 1 cut(s) 631
XspI CTAG 2 cut(s) 132, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.