Prupe.7G045400_v2.0.a1

Belongs to the pyrroline-5-carboxylate reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0024418
Physical Location & Seq
Forward (+)
8139650 .. 8144469
4820 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G045400.1

Sequence Viewer

Length: 852 bp
ATGGCAGGTGAGGGAGTGTCACCACTAGCACAAGTCAACCCAATTGACAAGGAAGCCTTCAAGTTGGGGTTCATTGGGGCTGGTAACTTGGCATCAAGTGTTGCAACCGGATTGGTCAAGCAGGGGATACTACCAGCTTCAAGAATTTTCACGGCACACCGGAACCCGGAAAGCAGGGTGGATTTTACATCCATGGGAATTAAGGTTTTCCCGAAAAATCATCAGGTAGTTGAAGAGTGTGATGTAATCATTTTCGCAGTGAAACCTCAAATACTTAAGGAAGTAATCTTGGACTTACGGCCACTACTTTCACAAAATAAACTTCTTCTGTCACTTGCTGTGGGACATAAATTGCAAGCTCTACAGGAATGGGTCGGTCATGGTAGATTTGTTAGAGTAATCCCTACTACTACTTCTATCATTGGTGAGGCAGCTACAGTTATGCACTTGGGAGAAGCTGCAACAGAAAACGACGGAGAGCTAATAGCAGATCTATTTGGAGCTGTTGGCAAGGTGTGGAAAATGGATGAGAAATTGTCTGATGCTGCTGCTGCTGTCGGAGGTGCTGGTACGGCATTTGTATATATAGCAATAGAAGCTATGTCTGATGGAGGTGTAGCCGCAGGCTTACCACGAGAAATTGCTTTGAGTCTTGCTGCTCAAGCTTTCTATGGAGCAGCTGCCATGGTAATGAAAACAGGAAAACACCCAGGTCAGCTGAAAGACCAAGTTGCTTCACCAGGAGGAACCACCATTGCTGGGATTCATGAGCTTGAGAAGGGTGCTTTTCGCGCGAGCATGATGAATGCGGTTGTTGCTACTACCAAGCGTAGCCATGAGATGGGCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 ko01110 ko01130 ko01230 map00330 map01100 map01110 map01130 map01230
Pfam Domains
Protein Families
F420_oxidored P5CR_dimer

Protein Analysis

284

Amino Acids

29.68

Weight (kDa)

6.72

Isoelectric Point (pI)

31.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024418)

Species Orthologous Gene IDs
malus_domestica MD12G1091800.v1.1
prunus_persica Prupe.7G045400_v2.0.a1
pyrus_communis pycom12g07750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 841
AccII CGCG 2 cut(s) 792, 794
AciI CCGC 2 cut(s) 621, 809
AcoI YGGCCR 1 cut(s) 299
AcsI RAATTY 1 cut(s) 144
AfaI GTAC 1 cut(s) 571
AfiI CCNNNNNNNGG 3 cut(s) 166, 759, 841
AflII CTTAAG 1 cut(s) 275
AgsI TTSAA 3 cut(s) 61, 141, 233
AjnI CCWGG 2 cut(s) 709, 739
AoxI GGCC 2 cut(s) 299, 844
ApeKI GCWGC 8 cut(s) 431, 458, 545, 548, 551, 656, 677, 680
ApoI RAATTY 1 cut(s) 144
AspLEI GCGC 1 cut(s) 794
AspS9I GGNCC 1 cut(s) 844
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 4 cut(s) 12, 20, 437, 729
BauI CACGAG 1 cut(s) 633
BbvI GCAGC 8 cut(s) 443, 445, 532, 535, 538, 643, 667, 689
BccI CCATC 2 cut(s) 602, 835
BceAI ACGGC 3 cut(s) 168, 314, 588
BciT130I CCWGG 2 cut(s) 711, 741
BciVI GTATCC 1 cut(s) 120
BcnI CCSGG 1 cut(s) 167
BfaI CTAG 1 cut(s) 26
BfmI CTRYAG 2 cut(s) 362, 435
BfrI CTTAAG 1 cut(s) 275
BfuI GTATCC 1 cut(s) 120
BglII AGATCT 1 cut(s) 490
BisI GCNGC 9 cut(s) 432, 459, 546, 549, 552, 621, 657, 678, 681
BlsI GCNGC 9 cut(s) 433, 460, 547, 550, 553, 622, 658, 679, 682
Bme1390I CCNGG 3 cut(s) 167, 711, 741
BmgT120I GGNCC 1 cut(s) 844
BmiI GGNNCC 2 cut(s) 164, 748
BmrFI CCNGG 3 cut(s) 167, 711, 741
BmsI GCATC 2 cut(s) 101, 532
BpuEI CTTGAG 2 cut(s) 645, 794
BpuMI CCSGG 1 cut(s) 167
BsaJI CCNNGG 3 cut(s) 192, 684, 709
BsaWI WCCGGW 2 cut(s) 107, 159
Bsc4I CCNNNNNNNGG 3 cut(s) 166, 759, 841
Bse3DI GCAATG 1 cut(s) 753
BseBI CCWGG 2 cut(s) 711, 741
BseDI CCNNGG 3 cut(s) 192, 684, 709
BseGI GGATG 2 cut(s) 188, 532
BseLI CCNNNNNNNGG 3 cut(s) 166, 759, 841
BseMI GCAATG 1 cut(s) 753
BseXI GCAGC 8 cut(s) 443, 445, 532, 535, 538, 643, 667, 689
BseYI CCCAGC 1 cut(s) 758
Bsh1236I CGCG 2 cut(s) 792, 794
BshFI GGCC 2 cut(s) 301, 846
BsiSI CCGG 3 cut(s) 108, 160, 167
BslFI GGGAC 1 cut(s) 357
BslI CCNNNNNNNGG 3 cut(s) 166, 759, 841
BsmFI GGGAC 1 cut(s) 357
BsmI GAATGC 1 cut(s) 811
BsnI GGCC 2 cut(s) 301, 846
Bsp143I GATC 1 cut(s) 490
Bsp19I CCATGG 2 cut(s) 192, 684
BspACI CCGC 2 cut(s) 621, 809
BspANI GGCC 2 cut(s) 301, 846
BspFNI CGCG 2 cut(s) 792, 794
BspHI TCATGA 1 cut(s) 766
BspLI GGNNCC 2 cut(s) 164, 748
BspTI CTTAAG 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 753
BssECI CCNNGG 3 cut(s) 192, 684, 709
BssMI GATC 1 cut(s) 490
BssSI CACGAG 1 cut(s) 633
BssT1I CCWWGG 2 cut(s) 192, 684
Bst2BI CACGAG 1 cut(s) 633
Bst2UI CCWGG 2 cut(s) 711, 741
Bst4CI ACNGT 1 cut(s) 439
Bst6I CTCTTC 1 cut(s) 228
BstAFI CTTAAG 1 cut(s) 275
BstC8I GCNNGC 3 cut(s) 357, 625, 796
BstDSI CCRYGG 2 cut(s) 192, 684
BstF5I GGATG 2 cut(s) 188, 532
BstFNI CGCG 2 cut(s) 792, 794
BstHHI GCGC 1 cut(s) 794
BstKTI GATC 1 cut(s) 493
BstMBI GATC 1 cut(s) 490
BstMWI GCNNNNNNNGC 6 cut(s) 551, 572, 596, 662, 791, 815
BstNI CCWGG 2 cut(s) 711, 741
BstSCI CCNGG 3 cut(s) 165, 709, 739
BstSFI CTRYAG 2 cut(s) 362, 435
BstUI CGCG 2 cut(s) 792, 794
BstV1I GCAGC 8 cut(s) 443, 445, 532, 535, 538, 643, 667, 689
BstX2I RGATCY 1 cut(s) 490
BstYI RGATCY 1 cut(s) 490
BsuI GTATCC 1 cut(s) 120
BsuRI GGCC 2 cut(s) 301, 846
BtgI CCRYGG 2 cut(s) 192, 684
BtsCI GGATG 2 cut(s) 188, 532
BtsI GCAGTG 1 cut(s) 264
BtsIMutI CAGTG 1 cut(s) 264
Cac8I GCNNGC 3 cut(s) 357, 625, 796
CciI TCATGA 1 cut(s) 766
CfoI GCGC 1 cut(s) 794
Cfr13I GGNCC 1 cut(s) 844
Csp6I GTAC 1 cut(s) 570
CviAII CATG 6 cut(s) 193, 380, 685, 767, 799, 836
CviQI GTAC 1 cut(s) 570
DpnI GATC 1 cut(s) 492
DpnII GATC 1 cut(s) 490
EaeI YGGCCR 1 cut(s) 299
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
Eco130I CCWWGG 2 cut(s) 192, 684
EcoRII CCWGG 2 cut(s) 709, 739
EcoT14I CCWWGG 2 cut(s) 192, 684
ErhI CCWWGG 2 cut(s) 192, 684
FaeI CATG 6 cut(s) 196, 383, 688, 770, 802, 839
FalI AAGNNNNNCTT 2 cut(s) 41, 73
FaqI GGGAC 1 cut(s) 357
FatI CATG 6 cut(s) 192, 379, 684, 766, 798, 835
Fnu4HI GCNGC 9 cut(s) 432, 459, 546, 549, 552, 621, 657, 678, 681
FokI GGATG 2 cut(s) 175, 539
Fsp4HI GCNGC 9 cut(s) 432, 459, 546, 549, 552, 621, 657, 678, 681
FspBI CTAG 1 cut(s) 26
GlaI GCGC 1 cut(s) 793
GluI GCNGC 9 cut(s) 432, 459, 546, 549, 552, 621, 657, 678, 681
GsaI CCCAGC 1 cut(s) 762
HaeIII GGCC 2 cut(s) 301, 846
HapII CCGG 3 cut(s) 108, 160, 167
HhaI GCGC 1 cut(s) 794
Hin1II CATG 6 cut(s) 196, 383, 688, 770, 802, 839
Hin6I GCGC 1 cut(s) 792
HinP1I GCGC 1 cut(s) 792
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HindIII AAGCTT 1 cut(s) 663
HinfI GANTC 2 cut(s) 649, 763
HpaII CCGG 3 cut(s) 108, 160, 167
HphI GGTGA 4 cut(s) 12, 20, 437, 729
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 3 cut(s) 541, 560, 607
Hpy188III TCNNGA 3 cut(s) 141, 211, 767
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 1 cut(s) 476
HpyAV CCTTC 2 cut(s) 67, 772
HpyCH4III ACNGT 1 cut(s) 439
HpyCH4V TGCA 4 cut(s) 104, 355, 445, 461
HpyF10VI GCNNNNNNNGC 6 cut(s) 551, 572, 596, 662, 791, 815
Hsp92II CATG 6 cut(s) 196, 383, 688, 770, 802, 839
HspAI GCGC 1 cut(s) 792
Kzo9I GATC 1 cut(s) 490
LmnI GCTCC 2 cut(s) 500, 674
Lsp1109I GCAGC 8 cut(s) 443, 445, 532, 535, 538, 643, 667, 689
LweI GCATC 2 cut(s) 101, 532
MaeI CTAG 1 cut(s) 26
MaeIII GTNAC 3 cut(s) 18, 83, 330
MalI GATC 1 cut(s) 492
MboI GATC 1 cut(s) 490
MboII GAAGA 2 cut(s) 245, 317
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 1 cut(s) 490
MluCI AATT 6 cut(s) 42, 144, 198, 350, 533, 639
MlyI GAGTC 1 cut(s) 658
MmeI TCCRAC 1 cut(s) 538
MnlI CCTC 6 cut(s) 4, 276, 421, 554, 605, 737
MseI TTAA 2 cut(s) 201, 276
MslI CAYNNNNRTG 1 cut(s) 689
MspA1I CMGCKG 2 cut(s) 680, 718
MspCI CTTAAG 1 cut(s) 275
MspI CCGG 3 cut(s) 108, 160, 167
MspR9I CCNGG 3 cut(s) 167, 711, 741
MunI CAATTG 1 cut(s) 42
Mva1269I GAATGC 1 cut(s) 811
MvaI CCWGG 2 cut(s) 711, 741
MvnI CGCG 2 cut(s) 792, 794
MwoI GCNNNNNNNGC 6 cut(s) 551, 572, 596, 662, 791, 815
NciI CCSGG 1 cut(s) 167
NcoI CCATGG 2 cut(s) 192, 684
NdeII GATC 1 cut(s) 490
NlaIII CATG 6 cut(s) 196, 383, 688, 770, 802, 839
NlaIV GGNNCC 2 cut(s) 164, 748
NmuCI GTSAC 2 cut(s) 18, 330
PagI TCATGA 1 cut(s) 766
PctI GAATGC 1 cut(s) 811
PfeI GAWTC 1 cut(s) 763
PflMI CCANNNNNTGG 1 cut(s) 841
PkrI GCNGC 9 cut(s) 433, 460, 547, 550, 553, 622, 658, 679, 682
PleI GAGTC 1 cut(s) 657
PpsI GAGTC 1 cut(s) 657
Psp6I CCWGG 2 cut(s) 709, 739
PspFI CCCAGC 1 cut(s) 758
PspGI CCWGG 2 cut(s) 709, 739
PspN4I GGNNCC 2 cut(s) 164, 748
PspPI GGNCC 1 cut(s) 844
PsuI RGATCY 1 cut(s) 490
PvuII CAGCTG 2 cut(s) 680, 718
RsaI GTAC 1 cut(s) 571
RsaNI GTAC 1 cut(s) 570
RseI CAYNNNNRTG 1 cut(s) 689
SaqAI TTAA 2 cut(s) 201, 276
SatI GCNGC 9 cut(s) 432, 459, 546, 549, 552, 621, 657, 678, 681
Sau3AI GATC 1 cut(s) 490
Sau96I GGNCC 1 cut(s) 844
SchI GAGTC 1 cut(s) 658
ScrFI CCNGG 3 cut(s) 167, 711, 741
SfaNI GCATC 2 cut(s) 101, 532
SfcI CTRYAG 2 cut(s) 362, 435
SmiMI CAYNNNNRTG 1 cut(s) 689
SmlI CTYRAG 3 cut(s) 275, 660, 773
SmoI CTYRAG 3 cut(s) 275, 660, 773
Sse9I AATT 6 cut(s) 42, 144, 198, 350, 533, 639
SsiI CCGC 2 cut(s) 621, 809
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 3 cut(s) 165, 709, 739
StyI CCWWGG 2 cut(s) 192, 684
TaaI ACNGT 1 cut(s) 439
TaqII GACCGA 1 cut(s) 365
TasI AATT 6 cut(s) 42, 144, 198, 350, 533, 639
TauI GCSGC 1 cut(s) 623
TfiI GAWTC 1 cut(s) 763
Tru1I TTAA 2 cut(s) 201, 276
Tru9I TTAA 2 cut(s) 201, 276
TscAI CASTG 1 cut(s) 264
TseFI GTSAC 2 cut(s) 18, 330
TseI GCWGC 8 cut(s) 431, 458, 545, 548, 551, 656, 677, 680
Tsp45I GTSAC 2 cut(s) 18, 330
TspDTI ATGAA 4 cut(s) 61, 707, 755, 818
TspGWI ACGGA 1 cut(s) 489
TspRI CASTG 1 cut(s) 264
Van91I CCANNNNNTGG 1 cut(s) 841
Vha464I CTTAAG 1 cut(s) 275
XapI RAATTY 1 cut(s) 144
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.