MD12G1105100.v1.1

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0024420
Physical Location & Seq
Reverse (-)
16570671 .. 16575745
5075 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1105100.v1.1.491

Sequence Viewer

Length: 708 bp
ATGCATGCTGTTGTTGTTGGCACGTATTTCGTTTTCGGTATAGTTGCTGGTGCCGTCAACATATATGCTAATGATCAAGGGAAGGATCGTGCGTCTGGCTGCAACGAGTCTGATAAGTGGGTGGGCAATATTATATGTGCGTTCAACAGGACTGCTAGTAACTTATATTTTACTAGTGTGACTTTATCAACTGTTGGTTACGGAGACATCGTTCCCCGTGACGGAGATAAAGTTGCCATGTTGCTGACAATGCTCTTGATGATATTGGGTTACTACATTGTGAGTTTTCTAATTGCCGCGGAAGTTGACAGATTGGGTTGTTGGTTATTGATGCGCTTCAACGAGAGAAGAAGAGGATTCATAAGATTTTACTCGGCATGTCTTTGCGGGCCAATACTCATAGCTTCTGGAGGTGTTATGATTAGGTATATGGAGGAGATTTCGTGGGCCGATTCCTTCTATCTATCAGTGGCGTCTGTCACAACTACAGGGTACGGGGATAAGCATTTCAAAACATTGTTGGGCAAGTGTTTTGCTAGCATATGGCTTCTGGTCAGCACCATCCTGAATAGTAAATCTGCACAGTTTTTGTTTGACGCGACCATGCGGATAGAAAGAGTGAGATCTTGTGGTGACTGTGGCACCATTTGCCACACATGTGCCGTCGCGGACTTAACCTCACAAATACACGCAGAACGCATTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

236

Amino Acids

25.91

Weight (kDa)

8.02

Isoelectric Point (pI)

32.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans_2 PF07885 53 - 101 2.1e-10 Ion channel
Ion_trans_2 PF07885 133 - 191 5.9e-11 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024420)

Species Orthologous Gene IDs
malus_domestica MD12G1105100.v1.1
pyrus_communis pycom12562g00030 pycom12g10380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 50, 641
AccII CGCG 3 cut(s) 299, 599, 668
AciI CCGC 5 cut(s) 297, 299, 387, 607, 668
AclWI GGATC 1 cut(s) 93
AcyI GRCGYC 1 cut(s) 473
AfaI GTAC 1 cut(s) 494
AfiI CCNNNNNNNGG 1 cut(s) 221
AflIII ACRYGT 1 cut(s) 656
AgsI TTSAA 3 cut(s) 145, 340, 511
AhlI ACTAGT 1 cut(s) 173
AleI CACNNNNGTG 1 cut(s) 657
AloI GAACNNNNNNTCC 2 cut(s) 195, 227
AluBI AGCT 1 cut(s) 404
AluI AGCT 1 cut(s) 404
Alw26I GTCTC 1 cut(s) 198
AlwI GGATC 1 cut(s) 93
AoxI GGCC 2 cut(s) 389, 447
ApeKI GCWGC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 336
AspS9I GGNCC 2 cut(s) 389, 447
AsuHPI GGTGA 1 cut(s) 644
AsuNHI GCTAGC 1 cut(s) 536
BanI GGYRCC 2 cut(s) 50, 641
BbvI GCAGC 1 cut(s) 86
BccI CCATC 1 cut(s) 569
BceAI ACGGC 2 cut(s) 38, 647
BcgI CGANNNNNNTGC 2 cut(s) 10, 44
BclI TGATCA 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 198
BcuI ACTAGT 1 cut(s) 173
BfaI CTAG 3 cut(s) 156, 174, 537
BfmI CTRYAG 1 cut(s) 486
BglII AGATCT 1 cut(s) 623
BisI GCNGC 2 cut(s) 100, 297
BlsI GCNGC 2 cut(s) 101, 298
BmgT120I GGNCC 2 cut(s) 389, 447
BmiI GGNNCC 2 cut(s) 52, 643
BmsI GCATC 1 cut(s) 321
BmtI GCTAGC 1 cut(s) 540
BpmI CTGGAG 1 cut(s) 429
BsaAI YACGTR 1 cut(s) 24
BsaHI GRCGYC 1 cut(s) 473
BsaJI CCNNGG 1 cut(s) 297
BsaXI ACNNNNNCTCC 6 cut(s) 195, 216, 225, 246, 428, 458
Bsc4I CCNNNNNNNGG 1 cut(s) 221
BseDI CCNNGG 1 cut(s) 297
BseGI GGATG 1 cut(s) 561
BseLI CCNNNNNNNGG 1 cut(s) 221
BseRI GAGGAG 1 cut(s) 449
BseXI GCAGC 1 cut(s) 86
BsgI GTGCAG 1 cut(s) 564
Bsh1236I CGCG 3 cut(s) 299, 599, 668
BshFI GGCC 2 cut(s) 391, 449
BshNI GGYRCC 2 cut(s) 50, 641
BslI CCNNNNNNNGG 1 cut(s) 221
BsmAI GTCTC 1 cut(s) 198
BsnI GGCC 2 cut(s) 391, 449
Bsp143I GATC 3 cut(s) 73, 85, 623
BspACI CCGC 5 cut(s) 297, 299, 387, 607, 668
BspANI GGCC 2 cut(s) 391, 449
BspFNI CGCG 3 cut(s) 299, 599, 668
BspLI GGNNCC 2 cut(s) 52, 643
BspOI GCTAGC 1 cut(s) 540
BspPI GGATC 1 cut(s) 93
BspT107I GGYRCC 2 cut(s) 50, 641
BssECI CCNNGG 1 cut(s) 297
BssMI GATC 3 cut(s) 73, 85, 623
BssNI GRCGYC 1 cut(s) 473
Bst4CI ACNGT 3 cut(s) 193, 585, 638
Bst6I CTCTTC 1 cut(s) 346
BstACI GRCGYC 1 cut(s) 473
BstAPI GCANNNNNTGC 1 cut(s) 648
BstBAI YACGTR 1 cut(s) 24
BstC8I GCNNGC 3 cut(s) 6, 389, 538
BstDSI CCRYGG 1 cut(s) 297
BstF5I GGATG 1 cut(s) 561
BstFNI CGCG 3 cut(s) 299, 599, 668
BstHHI GCGC 1 cut(s) 336
BstKTI GATC 3 cut(s) 76, 88, 626
BstMAI GTCTC 1 cut(s) 198
BstMBI GATC 3 cut(s) 73, 85, 623
BstMWI GCNNNNNNNGC 2 cut(s) 250, 648
BstNSI RCATGY 3 cut(s) 8, 381, 660
BstSFI CTRYAG 1 cut(s) 486
BstUI CGCG 3 cut(s) 299, 599, 668
BstV1I GCAGC 1 cut(s) 86
BstX2I RGATCY 1 cut(s) 623
BstYI RGATCY 1 cut(s) 623
BsuRI GGCC 2 cut(s) 391, 449
BtgI CCRYGG 1 cut(s) 297
BtsCI GGATG 1 cut(s) 561
BtsIMutI CAGTG 1 cut(s) 474
Cac8I GCNNGC 3 cut(s) 6, 389, 538
CfoI GCGC 1 cut(s) 336
Cfr13I GGNCC 2 cut(s) 389, 447
Cfr42I CCGCGG 1 cut(s) 300
CseI GACGC 3 cut(s) 81, 462, 605
Csp6I GTAC 1 cut(s) 493
CviAII CATG 5 cut(s) 5, 238, 378, 604, 657
CviJI RGCY 5 cut(s) 99, 391, 404, 449, 547
CviKI_1 RGCY 5 cut(s) 99, 391, 404, 449, 547
CviQI GTAC 1 cut(s) 493
DpnI GATC 3 cut(s) 75, 87, 625
DpnII GATC 3 cut(s) 73, 85, 623
Eam1104I CTCTTC 1 cut(s) 346
EarI CTCTTC 1 cut(s) 346
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 5 cut(s) 8, 241, 381, 607, 660
FatI CATG 5 cut(s) 4, 237, 377, 603, 656
FauI CCCGC 1 cut(s) 380
FauNDI CATATG 1 cut(s) 542
FbaI TGATCA 1 cut(s) 73
Fnu4HI GCNGC 2 cut(s) 100, 297
FokI GGATG 1 cut(s) 548
Fsp4HI GCNGC 2 cut(s) 100, 297
FspBI CTAG 3 cut(s) 156, 174, 537
GlaI GCGC 1 cut(s) 335
GluI GCNGC 2 cut(s) 100, 297
GsuI CTGGAG 1 cut(s) 429
HaeIII GGCC 2 cut(s) 391, 449
HgaI GACGC 3 cut(s) 81, 462, 605
HhaI GCGC 1 cut(s) 336
Hin1I GRCGYC 1 cut(s) 473
Hin1II CATG 5 cut(s) 8, 241, 381, 607, 660
Hin6I GCGC 1 cut(s) 334
HinP1I GCGC 1 cut(s) 334
HincII GTYRAC 2 cut(s) 58, 307
HindII GTYRAC 2 cut(s) 58, 307
HinfI GANTC 3 cut(s) 107, 357, 452
HphI GGTGA 1 cut(s) 644
Hpy166II GTNNAC 2 cut(s) 58, 307
Hpy188I TCNGA 1 cut(s) 112
Hpy188III TCNNGA 3 cut(s) 256, 408, 565
Hpy8I GTNNAC 2 cut(s) 58, 307
Hpy99I CGWCG 1 cut(s) 668
HpyAV CCTTC 2 cut(s) 76, 466
HpyCH4III ACNGT 3 cut(s) 193, 585, 638
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 3 cut(s) 4, 102, 581
HpyF10VI GCNNNNNNNGC 2 cut(s) 250, 648
HpySE526I ACGT 1 cut(s) 23
Hsp92I GRCGYC 1 cut(s) 473
Hsp92II CATG 5 cut(s) 8, 241, 381, 607, 660
HspAI GCGC 1 cut(s) 334
Ksp22I TGATCA 1 cut(s) 73
KspI CCGCGG 1 cut(s) 300
Kzo9I GATC 3 cut(s) 73, 85, 623
LpnPI CCDG 7 cut(s) 33, 81, 133, 393, 474, 536, 578
Lsp1109I GCAGC 1 cut(s) 86
LweI GCATC 1 cut(s) 321
MaeI CTAG 3 cut(s) 156, 174, 537
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 7 cut(s) 158, 178, 197, 218, 269, 478, 632
MalI GATC 3 cut(s) 75, 87, 625
MboI GATC 3 cut(s) 73, 85, 623
MboII GAAGA 2 cut(s) 360, 363
MflI RGATCY 1 cut(s) 623
MluCI AATT 1 cut(s) 291
MlyI GAGTC 1 cut(s) 116
MnlI CCTC 4 cut(s) 347, 404, 427, 688
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 674
MslI CAYNNNNRTG 1 cut(s) 657
MspA1I CMGCKG 1 cut(s) 299
MvnI CGCG 3 cut(s) 299, 599, 668
MwoI GCNNNNNNNGC 2 cut(s) 250, 648
NdeI CATATG 1 cut(s) 542
NdeII GATC 3 cut(s) 73, 85, 623
NheI GCTAGC 1 cut(s) 536
NlaIII CATG 5 cut(s) 8, 241, 381, 607, 660
NlaIV GGNNCC 2 cut(s) 52, 643
NmeAIII GCCGAG 1 cut(s) 353
NmuCI GTSAC 4 cut(s) 178, 218, 478, 632
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 8, 381, 660
OliI CACNNNNGTG 1 cut(s) 657
PaeI GCATGC 1 cut(s) 8
PciI ACATGT 1 cut(s) 656
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PfeI GAWTC 2 cut(s) 357, 452
PkrI GCNGC 2 cut(s) 101, 298
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
Ppu21I YACGTR 1 cut(s) 24
PscI ACATGT 1 cut(s) 656
PspN4I GGNNCC 2 cut(s) 52, 643
PspPI GGNCC 2 cut(s) 389, 447
PsuI RGATCY 1 cut(s) 623
RsaI GTAC 1 cut(s) 494
RsaNI GTAC 1 cut(s) 493
RseI CAYNNNNRTG 1 cut(s) 657
SacII CCGCGG 1 cut(s) 300
SaqAI TTAA 1 cut(s) 674
SatI GCNGC 2 cut(s) 100, 297
Sau3AI GATC 3 cut(s) 73, 85, 623
Sau96I GGNCC 2 cut(s) 389, 447
SchI GAGTC 1 cut(s) 116
SetI ASST 5 cut(s) 26, 406, 415, 428, 680
SfaNI GCATC 1 cut(s) 321
SfcI CTRYAG 1 cut(s) 486
Sfr303I CCGCGG 1 cut(s) 300
SgrBI CCGCGG 1 cut(s) 300
SmiMI CAYNNNNRTG 1 cut(s) 657
SpeI ACTAGT 1 cut(s) 173
SphI GCATGC 1 cut(s) 8
Sse9I AATT 1 cut(s) 291
SsiI CCGC 5 cut(s) 297, 299, 387, 607, 668
SspI AATATT 1 cut(s) 130
SspMI CTAG 3 cut(s) 156, 174, 537
TaaI ACNGT 3 cut(s) 193, 585, 638
TaiI ACGT 1 cut(s) 26
TasI AATT 1 cut(s) 291
TauI GCSGC 1 cut(s) 299
TfiI GAWTC 2 cut(s) 357, 452
Tru1I TTAA 1 cut(s) 674
Tru9I TTAA 1 cut(s) 674
TscAI CASTG 1 cut(s) 474
TseFI GTSAC 4 cut(s) 178, 218, 478, 632
TseI GCWGC 1 cut(s) 99
Tsp45I GTSAC 4 cut(s) 178, 218, 478, 632
TspDTI ATGAA 1 cut(s) 349
TspGWI ACGGA 2 cut(s) 216, 237
TspRI CASTG 1 cut(s) 474
XceI RCATGY 3 cut(s) 8, 381, 660
XspI CTAG 3 cut(s) 156, 174, 537
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.