pycom12562g00030

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012562
OG0024420
Physical Location & Seq
Reverse (-)
39019 .. 39801
783 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12562g00030.1

Sequence Viewer

Length: 783 bp
ATGTCTGGTTTTTCTTTAATTTTAGTTATGTCTCTACTGCTGCGAGAGCCTATTGCAGCTTTTGGGAGCATCGCTATCCATGCTGCTGTTGTTGCCACGTATTTTGTTTTCGGTATAGTTGCCGGTGCCTTCAACATGCATGCTAAGGATCGAGTGAAGGATCATGCGTCTGGCTGCAGCGAGTCTGATAATTGGGTGGGGGATATTATATTTGTGTTCAACCAGACTGCTAGTTACTTATATTTTACCAGCGTGACTTTATCAACTGTTGGTTACGGAGACATAGTTCCCCATGACGGAGATAAAGTTGCCATGTTGCTGACAATGCTCTTGATGTTATTGGGTTACTACCTTGTCAGTTTTGTGATTGCCATGGAAGTTGACAGATTGGGTAGTTGGTTGTTGATGCGCTTCAATGAGAGAAGAAGAGGATTCATAAGATTTTTCTGGGCATGTCTTTGCGGGCCGATGCTCATAGCTTCTGGAGGCGTTATGATTAGGTATATGGAGGGGATTTCGTGGGCCGATTCCTTCTATCTATCAGTGGCGTCTGTCACAACTACAGGGTACGGGGATAAGCATTTCAACACATTGTTGGGCAAGTGTTTTGCTAGCATATGGCTTCTGGTCAGCACCATCCTGAGTAGTAAATCTGCACAGTTTATGTTTGACGTGATCATGCGGATTAAAAGAGTGAGAGCTTGTCGTGCCTGTGGCACCATTTGCCACACATGTGCTGTCGCGGACTTAACATCACAAATACACGCAGTACGCATTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

261

Amino Acids

28.72

Weight (kDa)

8.63

Isoelectric Point (pI)

28.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans_2 PF07885 75 - 127 1.6e-11 Ion channel
Ion_trans_2 PF07885 158 - 226 4.9e-11 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024420)

Species Orthologous Gene IDs
malus_domestica MD12G1105100.v1.1
pyrus_communis pycom12562g00030 pycom12g10380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 125, 716
AccII CGCG 1 cut(s) 743
AciI CCGC 3 cut(s) 462, 682, 743
AclWI GGATC 2 cut(s) 156, 168
AcyI GRCGYC 1 cut(s) 548
AfaI GTAC 2 cut(s) 569, 771
AfiI CCNNNNNNNGG 1 cut(s) 296
AflIII ACRYGT 1 cut(s) 731
AgsI TTSAA 4 cut(s) 133, 220, 415, 586
AjiI CACGTC 1 cut(s) 673
AleI CACNNNNGTG 1 cut(s) 732
AloI GAACNNNNNNTCC 2 cut(s) 270, 302
AluBI AGCT 3 cut(s) 59, 479, 701
AluI AGCT 3 cut(s) 59, 479, 701
Alw26I GTCTC 2 cut(s) 36, 273
AlwI GGATC 2 cut(s) 156, 168
AoxI GGCC 2 cut(s) 464, 522
ApeKI GCWGC 5 cut(s) 40, 56, 83, 174, 177
AspLEI GCGC 1 cut(s) 411
AspS9I GGNCC 2 cut(s) 464, 522
AsuNHI GCTAGC 1 cut(s) 611
BanI GGYRCC 2 cut(s) 125, 716
BbvI GCAGC 5 cut(s) 27, 68, 70, 161, 189
BccI CCATC 1 cut(s) 644
BclI TGATCA 1 cut(s) 675
BcoDI GTCTC 2 cut(s) 36, 273
BfaI CTAG 2 cut(s) 231, 612
BfmI CTRYAG 2 cut(s) 175, 561
BisI GCNGC 5 cut(s) 41, 57, 84, 175, 178
BlsI GCNGC 5 cut(s) 42, 58, 85, 176, 179
BmgBI CACGTC 1 cut(s) 673
BmgT120I GGNCC 2 cut(s) 464, 522
BmiI GGNNCC 2 cut(s) 127, 718
BmsI GCATC 3 cut(s) 78, 396, 459
BmtI GCTAGC 1 cut(s) 615
BpmI CTGGAG 1 cut(s) 504
Bpu10I CCTNAGC 1 cut(s) 144
BsaAI YACGTR 1 cut(s) 99
BsaHI GRCGYC 1 cut(s) 548
BsaJI CCNNGG 1 cut(s) 372
BsaXI ACNNNNNCTCC 4 cut(s) 270, 291, 300, 321
Bsc4I CCNNNNNNNGG 1 cut(s) 296
Bse118I RCCGGY 1 cut(s) 122
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 1 cut(s) 636
BseLI CCNNNNNNNGG 1 cut(s) 296
BseMII CTCAG 1 cut(s) 632
BseXI GCAGC 5 cut(s) 27, 68, 70, 161, 189
BsgI GTGCAG 1 cut(s) 639
Bsh1236I CGCG 1 cut(s) 743
BshFI GGCC 2 cut(s) 466, 524
BshNI GGYRCC 2 cut(s) 125, 716
BsiSI CCGG 1 cut(s) 123
BslI CCNNNNNNNGG 1 cut(s) 296
BsmAI GTCTC 2 cut(s) 36, 273
BsnI GGCC 2 cut(s) 466, 524
Bsp143I GATC 3 cut(s) 148, 160, 675
Bsp19I CCATGG 1 cut(s) 372
BspACI CCGC 3 cut(s) 462, 682, 743
BspANI GGCC 2 cut(s) 466, 524
BspCNI CTCAG 1 cut(s) 633
BspFNI CGCG 1 cut(s) 743
BspLI GGNNCC 2 cut(s) 127, 718
BspMAI CTGCAG 1 cut(s) 179
BspOI GCTAGC 1 cut(s) 615
BspPI GGATC 2 cut(s) 156, 168
BspT107I GGYRCC 2 cut(s) 125, 716
BsrFI RCCGGY 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 3 cut(s) 148, 160, 675
BssNI GRCGYC 1 cut(s) 548
BssT1I CCWWGG 1 cut(s) 372
Bst4CI ACNGT 2 cut(s) 268, 660
Bst6I CTCTTC 1 cut(s) 421
BstACI GRCGYC 1 cut(s) 548
BstAPI GCANNNNNTGC 1 cut(s) 723
BstBAI YACGTR 1 cut(s) 99
BstC8I GCNNGC 3 cut(s) 141, 464, 613
BstDEI CTNAG 2 cut(s) 144, 641
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 1 cut(s) 636
BstFNI CGCG 1 cut(s) 743
BstHHI GCGC 1 cut(s) 411
BstKTI GATC 3 cut(s) 151, 163, 678
BstMAI GTCTC 2 cut(s) 36, 273
BstMBI GATC 3 cut(s) 148, 160, 675
BstMWI GCNNNNNNNGC 6 cut(s) 46, 80, 92, 325, 707, 723
BstNSI RCATGY 4 cut(s) 139, 143, 456, 735
BstSFI CTRYAG 2 cut(s) 175, 561
BstUI CGCG 1 cut(s) 743
BstV1I GCAGC 5 cut(s) 27, 68, 70, 161, 189
BsuRI GGCC 2 cut(s) 466, 524
BtgI CCRYGG 1 cut(s) 372
BtgZI GCGATG 1 cut(s) 55
BtrI CACGTC 1 cut(s) 673
BtsCI GGATG 1 cut(s) 636
BtsIMutI CAGTG 1 cut(s) 549
Cac8I GCNNGC 3 cut(s) 141, 464, 613
CfoI GCGC 1 cut(s) 411
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 2 cut(s) 464, 522
CseI GACGC 2 cut(s) 156, 537
Csp6I GTAC 2 cut(s) 568, 770
CspCI CAANNNNNGTGG 2 cut(s) 85, 120
CviJI RGCY 8 cut(s) 49, 59, 174, 466, 479, 524, 622, 701
CviKI_1 RGCY 8 cut(s) 49, 59, 174, 466, 479, 524, 622, 701
CviQI GTAC 2 cut(s) 568, 770
DdeI CTNAG 2 cut(s) 144, 641
DpnI GATC 3 cut(s) 150, 162, 677
DpnII GATC 3 cut(s) 148, 160, 675
Eam1104I CTCTTC 1 cut(s) 421
EarI CTCTTC 1 cut(s) 421
Eco130I CCWWGG 1 cut(s) 372
EcoT14I CCWWGG 1 cut(s) 372
EcoT22I ATGCAT 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 372
FauI CCCGC 1 cut(s) 455
FauNDI CATATG 1 cut(s) 617
FbaI TGATCA 1 cut(s) 675
Fnu4HI GCNGC 5 cut(s) 41, 57, 84, 175, 178
FokI GGATG 1 cut(s) 623
Fsp4HI GCNGC 5 cut(s) 41, 57, 84, 175, 178
FspBI CTAG 2 cut(s) 231, 612
GlaI GCGC 1 cut(s) 410
GluI GCNGC 5 cut(s) 41, 57, 84, 175, 178
GsuI CTGGAG 1 cut(s) 504
HaeIII GGCC 2 cut(s) 466, 524
HapII CCGG 1 cut(s) 123
HgaI GACGC 2 cut(s) 156, 537
HhaI GCGC 1 cut(s) 411
Hin1I GRCGYC 1 cut(s) 548
Hin6I GCGC 1 cut(s) 409
HinP1I GCGC 1 cut(s) 409
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 3 cut(s) 182, 432, 527
HpaII CCGG 1 cut(s) 123
Hpy166II GTNNAC 1 cut(s) 382
Hpy188I TCNGA 1 cut(s) 187
Hpy188III TCNNGA 3 cut(s) 331, 483, 640
Hpy8I GTNNAC 1 cut(s) 382
HpyAV CCTTC 3 cut(s) 139, 151, 541
HpyCH4III ACNGT 2 cut(s) 268, 660
HpyCH4IV ACGT 2 cut(s) 98, 672
HpyCH4V TGCA 4 cut(s) 56, 139, 177, 656
HpyF10VI GCNNNNNNNGC 6 cut(s) 46, 80, 92, 325, 707, 723
HpyF3I CTNAG 2 cut(s) 144, 641
HpySE526I ACGT 2 cut(s) 98, 672
Hsp92I GRCGYC 1 cut(s) 548
HspAI GCGC 1 cut(s) 409
Ksp22I TGATCA 1 cut(s) 675
Kzo9I GATC 3 cut(s) 148, 160, 675
LmnI GCTCC 1 cut(s) 66
Lsp1109I GCAGC 5 cut(s) 27, 68, 70, 161, 189
LweI GCATC 3 cut(s) 78, 396, 459
MaeI CTAG 2 cut(s) 231, 612
MaeII ACGT 2 cut(s) 98, 672
MaeIII GTNAC 5 cut(s) 233, 253, 272, 344, 553
MalI GATC 3 cut(s) 150, 162, 677
MboI GATC 3 cut(s) 148, 160, 675
MboII GAAGA 2 cut(s) 435, 438
MluCI AATT 2 cut(s) 18, 190
MlyI GAGTC 1 cut(s) 191
MnlI CCTC 3 cut(s) 422, 479, 502
Mph1103I ATGCAT 1 cut(s) 141
MseI TTAA 3 cut(s) 17, 687, 749
MslI CAYNNNNRTG 1 cut(s) 732
MspI CCGG 1 cut(s) 123
MvnI CGCG 1 cut(s) 743
MwoI GCNNNNNNNGC 6 cut(s) 46, 80, 92, 325, 707, 723
NcoI CCATGG 1 cut(s) 372
NdeI CATATG 1 cut(s) 617
NdeII GATC 3 cut(s) 148, 160, 675
NheI GCTAGC 1 cut(s) 611
NlaIV GGNNCC 2 cut(s) 127, 718
NmuCI GTSAC 2 cut(s) 253, 553
NsiI ATGCAT 1 cut(s) 141
NspI RCATGY 4 cut(s) 139, 143, 456, 735
OliI CACNNNNGTG 1 cut(s) 732
PaeI GCATGC 1 cut(s) 143
PciI ACATGT 1 cut(s) 731
PfeI GAWTC 2 cut(s) 432, 527
PkrI GCNGC 5 cut(s) 42, 58, 85, 176, 179
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
Ppu21I YACGTR 1 cut(s) 99
PscI ACATGT 1 cut(s) 731
PspN4I GGNNCC 2 cut(s) 127, 718
PspPI GGNCC 2 cut(s) 464, 522
PstI CTGCAG 1 cut(s) 179
RsaI GTAC 2 cut(s) 569, 771
RsaNI GTAC 2 cut(s) 568, 770
RseI CAYNNNNRTG 1 cut(s) 732
SaqAI TTAA 3 cut(s) 17, 687, 749
SatI GCNGC 5 cut(s) 41, 57, 84, 175, 178
Sau3AI GATC 3 cut(s) 148, 160, 675
Sau96I GGNCC 2 cut(s) 464, 522
SchI GAGTC 1 cut(s) 191
SetI ASST 7 cut(s) 61, 101, 354, 481, 503, 675, 703
SfaNI GCATC 3 cut(s) 78, 396, 459
SfcI CTRYAG 2 cut(s) 175, 561
SmiMI CAYNNNNRTG 1 cut(s) 732
SphI GCATGC 1 cut(s) 143
Sse9I AATT 2 cut(s) 18, 190
SsiI CCGC 3 cut(s) 462, 682, 743
SspMI CTAG 2 cut(s) 231, 612
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 2 cut(s) 268, 660
TaiI ACGT 2 cut(s) 101, 675
TaqI TCGA 1 cut(s) 151
TasI AATT 2 cut(s) 18, 190
TfiI GAWTC 2 cut(s) 432, 527
Tru1I TTAA 3 cut(s) 17, 687, 749
Tru9I TTAA 3 cut(s) 17, 687, 749
TscAI CASTG 1 cut(s) 549
TseFI GTSAC 2 cut(s) 253, 553
TseI GCWGC 5 cut(s) 40, 56, 83, 174, 177
Tsp45I GTSAC 2 cut(s) 253, 553
TspDTI ATGAA 1 cut(s) 424
TspGWI ACGGA 2 cut(s) 291, 312
TspRI CASTG 1 cut(s) 549
XceI RCATGY 4 cut(s) 139, 143, 456, 735
XspI CTAG 2 cut(s) 231, 612
Zsp2I ATGCAT 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.