MD12G1122700.v1.1

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Electrons are transferred to the Fe-S cluster from NADPH via a FAD- and FMN-containing diflavin oxidoreductase. Has anti- apoptotic effects in the cell

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0028725
Physical Location & Seq
Reverse (-)
19733862 .. 19735410
1549 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1122700.v1.1.491

Sequence Viewer

Length: 426 bp
ATGGCGAATCCGGCTTCGATCAAACCTGTGGATTTGCCAAAAATTGAGTTAGAAGATGATGGTCTGATTGATGAGGATAGTTTGTTGACAGAGGAGGATAGGAAGAGACCGGATCTACGTAAGGAATCTGTGGACTCGGGGATGCTTTTCGATGCTCTGCATGTCCTGTTAAGGGCAGGCTGTGCTGTTGATTTCACCGATTTCGAGAGGCATTGTGACTTTGACAGATGTAAACAAGAGGATGCTTTTCGTTGCTCTGCGTGTCCTTTCAAGGGTCTCCCAGTTTGGAAACAGGCAATAAGGTATTTGTTGGATCAAACTCAATCCTTCCCTCCCCCTGTCCCTCTCTTTGCCTCCGGTTTCATGTTTAATGTTTCCCTTTTCCGGGTACATTTGTCGGCCAAGTTCAGCGAACCAGACAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006790 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016226 GO:0022607 GO:0031163 GO:0044085 GO:0044237 GO:0044424 GO:0044464 GO:0051186 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIAPIN1 Methyltransf_11

Protein Analysis

142

Amino Acids

16.01

Weight (kDa)

4.81

Isoelectric Point (pI)

57.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CIAPIN1 PF05093 60 - 97 1.6e-06 Cytokine-induced anti-apoptosis inhibitor 1, Fe-S biogenesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028725)

Species Orthologous Gene IDs
malus_domestica MD12G1122700.v1.1
pyrus_communis pycom12g12150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 120, 321
AcoI YGGCCR 1 cut(s) 399
AfaI GTAC 1 cut(s) 390
AfiI CCNNNNNNNGG 4 cut(s) 172, 272, 384, 385
AgsI TTSAA 1 cut(s) 271
AluBI AGCT 1 cut(s) 423
AluI AGCT 1 cut(s) 423
Alw26I GTCTC 2 cut(s) 100, 281
AlwI GGATC 2 cut(s) 120, 321
Ama87I CYCGRG 1 cut(s) 136
AoxI GGCC 1 cut(s) 399
AsuC2I CCSGG 1 cut(s) 386
AsuHPI GGTGA 1 cut(s) 187
AvaI CYCGRG 1 cut(s) 136
BaeI ACNNNNGTAYC 2 cut(s) 380, 413
BccI CCATC 1 cut(s) 53
BcnI CCSGG 1 cut(s) 386
BcoDI GTCTC 2 cut(s) 100, 281
BfaI CTAG 1 cut(s) 424
Bme1390I CCNGG 1 cut(s) 386
BmeT110I CYCGRG 1 cut(s) 136
BmrFI CCNGG 1 cut(s) 386
BmrI ACTGGG 1 cut(s) 275
BmsI GCATC 3 cut(s) 132, 142, 232
BmuI ACTGGG 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 386
BsaAI YACGTR 1 cut(s) 119
BsaI GGTCTC 2 cut(s) 100, 281
BsaWI WCCGGW 2 cut(s) 109, 356
Bsc4I CCNNNNNNNGG 4 cut(s) 172, 272, 384, 385
Bse1I ACTGG 1 cut(s) 281
BseGI GGATG 2 cut(s) 147, 247
BseLI CCNNNNNNNGG 4 cut(s) 172, 272, 384, 385
BseNI ACTGG 1 cut(s) 281
BseRI GAGGAG 1 cut(s) 107
BshFI GGCC 1 cut(s) 401
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 4 cut(s) 11, 110, 357, 385
BslFI GGGAC 1 cut(s) 326
BslI CCNNNNNNNGG 4 cut(s) 172, 272, 384, 385
BsmAI GTCTC 2 cut(s) 100, 281
BsmFI GGGAC 1 cut(s) 326
BsnI GGCC 1 cut(s) 401
Bso31I GGTCTC 2 cut(s) 100, 281
BsoBI CYCGRG 1 cut(s) 136
Bsp143I GATC 3 cut(s) 18, 112, 313
BspANI GGCC 1 cut(s) 401
BspPI GGATC 2 cut(s) 120, 321
BspTNI GGTCTC 2 cut(s) 100, 281
BsrI ACTGG 1 cut(s) 281
BssMI GATC 3 cut(s) 18, 112, 313
Bst6I CTCTTC 1 cut(s) 98
BstAPI GCANNNNNTGC 1 cut(s) 182
BstBAI YACGTR 1 cut(s) 119
BstC8I GCNNGC 1 cut(s) 178
BstF5I GGATG 2 cut(s) 147, 247
BstKTI GATC 3 cut(s) 21, 115, 316
BstMAI GTCTC 2 cut(s) 100, 281
BstMBI GATC 3 cut(s) 18, 112, 313
BstMWI GCNNNNNNNGC 2 cut(s) 11, 182
BstNSI RCATGY 1 cut(s) 164
BstSCI CCNGG 1 cut(s) 384
BstSNI TACGTA 1 cut(s) 119
BstX2I RGATCY 1 cut(s) 112
BstYI RGATCY 1 cut(s) 112
BsuRI GGCC 1 cut(s) 401
BtsCI GGATG 2 cut(s) 147, 247
Cac8I GCNNGC 1 cut(s) 178
Csp6I GTAC 1 cut(s) 389
CviAII CATG 2 cut(s) 161, 364
CviJI RGCY 4 cut(s) 14, 180, 401, 423
CviKI_1 RGCY 4 cut(s) 14, 180, 401, 423
CviQI GTAC 1 cut(s) 389
DpnI GATC 3 cut(s) 20, 114, 315
DpnII GATC 3 cut(s) 18, 112, 313
EaeI YGGCCR 1 cut(s) 399
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
Eco105I TACGTA 1 cut(s) 119
Eco31I GGTCTC 2 cut(s) 100, 281
Eco88I CYCGRG 1 cut(s) 136
FaeI CATG 2 cut(s) 164, 367
FaiI YATR 2 cut(s) 162, 365
FaqI GGGAC 1 cut(s) 326
FatI CATG 2 cut(s) 160, 363
FokI GGATG 2 cut(s) 154, 254
FspBI CTAG 1 cut(s) 424
HaeIII GGCC 1 cut(s) 401
HapII CCGG 4 cut(s) 11, 110, 357, 385
Hin1II CATG 2 cut(s) 164, 367
HincII GTYRAC 1 cut(s) 87
HindII GTYRAC 1 cut(s) 87
HinfI GANTC 3 cut(s) 7, 125, 134
HpaII CCGG 4 cut(s) 11, 110, 357, 385
HphI GGTGA 1 cut(s) 187
Hpy166II GTNNAC 3 cut(s) 87, 133, 233
Hpy188I TCNGA 1 cut(s) 66
Hpy188III TCNNGA 1 cut(s) 205
Hpy8I GTNNAC 3 cut(s) 87, 133, 233
HpyAV CCTTC 1 cut(s) 337
HpyCH4IV ACGT 1 cut(s) 118
HpyCH4V TGCA 1 cut(s) 160
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 182
HpySE526I ACGT 1 cut(s) 118
Hsp92II CATG 2 cut(s) 164, 367
Kzo9I GATC 3 cut(s) 18, 112, 313
LweI GCATC 3 cut(s) 132, 142, 232
MaeI CTAG 1 cut(s) 424
MaeII ACGT 1 cut(s) 118
MaeIII GTNAC 1 cut(s) 215
MalI GATC 3 cut(s) 20, 114, 315
MboI GATC 3 cut(s) 18, 112, 313
MboII GAAGA 2 cut(s) 65, 115
MflI RGATCY 1 cut(s) 112
MluCI AATT 1 cut(s) 42
MlyI GAGTC 1 cut(s) 128
MmeI TCCRAC 1 cut(s) 291
MnlI CCTC 8 cut(s) 67, 85, 88, 201, 232, 342, 354, 364
MseI TTAA 2 cut(s) 170, 369
MspI CCGG 4 cut(s) 11, 110, 357, 385
MspR9I CCNGG 1 cut(s) 386
MwoI GCNNNNNNNGC 2 cut(s) 11, 182
NciI CCSGG 1 cut(s) 386
NdeII GATC 3 cut(s) 18, 112, 313
NlaIII CATG 2 cut(s) 164, 367
NmuCI GTSAC 1 cut(s) 215
NspI RCATGY 1 cut(s) 164
PfeI GAWTC 2 cut(s) 7, 125
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
Ppu21I YACGTR 1 cut(s) 119
PsuI RGATCY 1 cut(s) 112
RsaI GTAC 1 cut(s) 390
RsaNI GTAC 1 cut(s) 389
SaqAI TTAA 2 cut(s) 170, 369
Sau3AI GATC 3 cut(s) 18, 112, 313
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 1 cut(s) 386
SetI ASST 4 cut(s) 28, 121, 305, 425
SfaNI GCATC 3 cut(s) 132, 142, 232
SnaBI TACGTA 1 cut(s) 119
Sse9I AATT 1 cut(s) 42
SspMI CTAG 1 cut(s) 424
StyD4I CCNGG 1 cut(s) 384
TaiI ACGT 1 cut(s) 121
TaqI TCGA 3 cut(s) 17, 150, 204
TasI AATT 1 cut(s) 42
TfiI GAWTC 2 cut(s) 7, 125
Tru1I TTAA 2 cut(s) 170, 369
Tru9I TTAA 2 cut(s) 170, 369
TseFI GTSAC 1 cut(s) 215
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 1 cut(s) 352
XceI RCATGY 1 cut(s) 164
XspI CTAG 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.