MD12G1124900.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0028726
Physical Location & Seq
Reverse (-)
20001102 .. 20003519
2418 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1124900.v1.1.491

Sequence Viewer

Length: 930 bp
ATGAAGGATGAAAAGGAAATTGCGGTCAAAGTTCTAACGAGTAATTCCTACCAGGGAAAGCGAGAATTTTCAAATGAGGTAACTCTTCTTTCAAGGATACATCATAGAAACCTGGTACAGTTTCTTGGGTATTGCCAAGAGGATGGAAGAAGTATGCTGATCTACGAGTTCATGCATAATGGAACTCTCAAGGAACATCTTTATGGCCCATTAACACACAAACAGAGTATCAATTGGATCAAGCGCCTTGAGATTGCTGAAGATGCTGCAAAAGGAATCGAATACCTTCACACAGGCTGTGTTCCAGCTATCATTCATAGAGATTTGAAAAGCAGCAACATTCTAATTGACAGACACATGAGAGCAAAGGTTTCAGATTTTGGTCTTTCCAAACTTGAAGTAGACGGAGCATCCCATGTGTCAAGCAAAGTTCGGGGCACTGTAGGGTATCTGGATCCCGAGTATTACATCTCCCAGCAGTTGACAGACAAGAGTGACGTTTATAGTTTCGGTGTCGTTCTTCTTGAGCTGATATCAGGTCAAGAAGCGATTTCTAATAAACACTTTGGTGTTAACTGCCGTAGCATAGTGCAATGGGCAAAATTACACATTGAGAATGGCAACATTCAAGGAATAATCGACCCCTCGCTGGATGGCGAATACAACATCCAGTCAATGTGGAAGATAGCCGAAAAAGCCTTAATGTGTGTTCAAGCGCACGGATTTATGAGGCCGTCCATTTCAGAAGTTCTCAAGGAAATTCAAGATGCAATCTCAGTGGAAAGGGATGCTGCAGCAGTAAGAGAAGGTTCCTCAGATGTATCAAAGAATTCAATCCCTTCTTCCTTGGACACAGGTTCCCTGGATCTTGGCAGAAATGACAATTTATTGTCGATCGATGAGTCTATCGCCCGGCCAACTGCACGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

310

Amino Acids

34.57

Weight (kDa)

6.07

Isoelectric Point (pI)

35.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 2 - 202 1.6e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 3 - 185 8.8e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028726)

Species Orthologous Gene IDs
malus_domestica MD12G1124900.v1.1
pyrus_communis pycom04g09990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 402
AciI CCGC 1 cut(s) 23
AclWI GGATC 4 cut(s) 245, 449, 462, 873
AcoI YGGCCR 1 cut(s) 914
AcsI RAATTY 3 cut(s) 65, 759, 829
AcuI CTGAAG 1 cut(s) 279
AfaI GTAC 1 cut(s) 117
AfiI CCNNNNNNNGG 1 cut(s) 649
AgsI TTSAA 8 cut(s) 72, 93, 328, 398, 629, 713, 764, 834
AjnI CCWGG 3 cut(s) 51, 111, 861
AleI CACNNNNGTG 1 cut(s) 567
AluBI AGCT 2 cut(s) 308, 529
AluI AGCT 2 cut(s) 308, 529
AlwI GGATC 4 cut(s) 245, 449, 462, 873
Ama87I CYCGRG 1 cut(s) 458
AoxI GGCC 3 cut(s) 205, 731, 914
ApeKI GCWGC 4 cut(s) 266, 333, 791, 794
ApoI RAATTY 3 cut(s) 65, 759, 829
Asp700I GAANNNNTTC 1 cut(s) 285
AspLEI GCGC 2 cut(s) 246, 718
AspS9I GGNCC 1 cut(s) 206
AsuC2I CCSGG 1 cut(s) 913
AvaI CYCGRG 1 cut(s) 458
BaeGI GKGCMC 1 cut(s) 440
BamHI GGATCC 1 cut(s) 454
BbvI GCAGC 4 cut(s) 253, 345, 778, 806
BccI CCATC 2 cut(s) 137, 647
BceAI ACGGC 2 cut(s) 564, 718
BciT130I CCWGG 3 cut(s) 53, 113, 863
BciVI GTATCC 1 cut(s) 90
BcnI CCSGG 1 cut(s) 913
BfmI CTRYAG 2 cut(s) 441, 792
BfoI RGCGCY 1 cut(s) 247
BfuI GTATCC 1 cut(s) 90
BisI GCNGC 4 cut(s) 267, 334, 792, 795
BlsI GCNGC 4 cut(s) 268, 335, 793, 796
Bme1390I CCNGG 4 cut(s) 53, 113, 863, 913
BmeT110I CYCGRG 1 cut(s) 458
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 3 cut(s) 456, 811, 859
BmrFI CCNGG 4 cut(s) 53, 113, 863, 913
BmsI GCATC 4 cut(s) 253, 419, 757, 778
BpuEI CTTGAG 4 cut(s) 173, 269, 545, 737
BpuMI CCSGG 1 cut(s) 913
Bsa29I ATCGAT 1 cut(s) 897
BsaJI CCNNGG 3 cut(s) 52, 846, 861
Bsc4I CCNNNNNNNGG 1 cut(s) 649
Bse1I ACTGG 1 cut(s) 670
Bse3DI GCAATG 1 cut(s) 599
BseBI CCWGG 3 cut(s) 53, 113, 863
BseCI ATCGAT 1 cut(s) 897
BseDI CCNNGG 3 cut(s) 52, 846, 861
BseGI GGATG 6 cut(s) 13, 148, 410, 658, 666, 793
BseLI CCNNNNNNNGG 1 cut(s) 649
BseMI GCAATG 1 cut(s) 599
BseMII CTCAG 2 cut(s) 789, 828
BseNI ACTGG 1 cut(s) 670
BseSI GKGCMC 1 cut(s) 440
BseXI GCAGC 4 cut(s) 253, 345, 778, 806
BseYI CCCAGC 1 cut(s) 474
BsgI GTGCAG 1 cut(s) 906
Bsh1285I CGRYCG 1 cut(s) 897
BshFI GGCC 3 cut(s) 207, 733, 916
BshVI ATCGAT 1 cut(s) 897
BsiEI CGRYCG 1 cut(s) 897
BsiHKCI CYCGRG 1 cut(s) 458
BsiSI CCGG 1 cut(s) 913
BslI CCNNNNNNNGG 1 cut(s) 649
BsnI GGCC 3 cut(s) 207, 733, 916
BsoBI CYCGRG 1 cut(s) 458
Bsp1286I GDGCHC 1 cut(s) 440
Bsp143I GATC 5 cut(s) 159, 237, 454, 865, 894
BspACI CCGC 1 cut(s) 23
BspANI GGCC 3 cut(s) 207, 733, 916
BspCNI CTCAG 2 cut(s) 788, 827
BspDI ATCGAT 1 cut(s) 897
BspLI GGNNCC 3 cut(s) 456, 811, 859
BspMAI CTGCAG 1 cut(s) 796
BspPI GGATC 4 cut(s) 245, 449, 462, 873
BsrDI GCAATG 1 cut(s) 599
BsrI ACTGG 1 cut(s) 670
BssECI CCNNGG 3 cut(s) 52, 846, 861
BssMI GATC 5 cut(s) 159, 237, 454, 865, 894
BssT1I CCWWGG 1 cut(s) 846
Bst2UI CCWGG 3 cut(s) 53, 113, 863
Bst4CI ACNGT 2 cut(s) 120, 442
Bst6I CTCTTC 1 cut(s) 90
BstDEI CTNAG 2 cut(s) 775, 814
BstF5I GGATG 6 cut(s) 13, 148, 410, 658, 666, 793
BstH2I RGCGCY 1 cut(s) 247
BstHHI GCGC 2 cut(s) 246, 718
BstKTI GATC 5 cut(s) 162, 240, 457, 868, 897
BstMBI GATC 5 cut(s) 159, 237, 454, 865, 894
BstMCI CGRYCG 1 cut(s) 897
BstMWI GCNNNNNNNGC 2 cut(s) 263, 695
BstNI CCWGG 3 cut(s) 53, 113, 863
BstSCI CCNGG 4 cut(s) 51, 111, 861, 911
BstSFI CTRYAG 2 cut(s) 441, 792
BstSLI GKGCMC 1 cut(s) 440
BstV1I GCAGC 4 cut(s) 253, 345, 778, 806
BstX2I RGATCY 2 cut(s) 454, 865
BstXI CCANNNNNNTGG 1 cut(s) 143
BstYI RGATCY 2 cut(s) 454, 865
Bsu15I ATCGAT 1 cut(s) 897
BsuI GTATCC 1 cut(s) 90
BsuRI GGCC 3 cut(s) 207, 733, 916
BsuTUI ATCGAT 1 cut(s) 897
BtsCI GGATG 6 cut(s) 13, 148, 410, 658, 666, 793
BtsIMutI CAGTG 2 cut(s) 438, 783
CfoI GCGC 2 cut(s) 246, 718
Cfr13I GGNCC 1 cut(s) 206
ClaI ATCGAT 1 cut(s) 897
CsiI ACCWGGT 1 cut(s) 111
Csp6I GTAC 1 cut(s) 116
CspCI CAANNNNNGTGG 2 cut(s) 759, 794
CviAII CATG 3 cut(s) 172, 358, 416
CviJI RGCY 8 cut(s) 207, 297, 308, 529, 689, 698, 733, 916
CviKI_1 RGCY 8 cut(s) 207, 297, 308, 529, 689, 698, 733, 916
CviQI GTAC 1 cut(s) 116
DdeI CTNAG 2 cut(s) 775, 814
DpnI GATC 5 cut(s) 161, 239, 456, 867, 896
DpnII GATC 5 cut(s) 159, 237, 454, 865, 894
EaeI YGGCCR 1 cut(s) 914
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 846
Eco32I GATATC 1 cut(s) 534
Eco57I CTGAAG 1 cut(s) 279
Eco88I CYCGRG 1 cut(s) 458
EcoRI GAATTC 1 cut(s) 829
EcoRII CCWGG 3 cut(s) 51, 111, 861
EcoRV GATATC 1 cut(s) 534
EcoT14I CCWWGG 1 cut(s) 846
EcoT22I ATGCAT 1 cut(s) 177
ErhI CCWWGG 1 cut(s) 846
FaeI CATG 3 cut(s) 175, 361, 419
FatI CATG 3 cut(s) 171, 357, 415
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 4 cut(s) 267, 334, 792, 795
FokI GGATG 6 cut(s) 20, 155, 397, 653, 665, 800
Fsp4HI GCNGC 4 cut(s) 267, 334, 792, 795
GlaI GCGC 2 cut(s) 245, 717
GluI GCNGC 4 cut(s) 267, 334, 792, 795
GsaI CCCAGC 1 cut(s) 478
HaeII RGCGCY 1 cut(s) 247
HaeIII GGCC 3 cut(s) 207, 733, 916
HapII CCGG 1 cut(s) 913
HhaI GCGC 2 cut(s) 246, 718
Hin1II CATG 3 cut(s) 175, 361, 419
Hin6I GCGC 2 cut(s) 244, 716
HinP1I GCGC 2 cut(s) 244, 716
HincII GTYRAC 2 cut(s) 483, 574
HindII GTYRAC 2 cut(s) 483, 574
HinfI GANTC 2 cut(s) 276, 902
HpaI GTTAAC 1 cut(s) 574
HpaII CCGG 1 cut(s) 913
Hpy166II GTNNAC 3 cut(s) 403, 483, 574
Hpy188I TCNGA 3 cut(s) 376, 745, 817
Hpy188III TCNNGA 5 cut(s) 452, 458, 524, 542, 764
Hpy8I GTNNAC 3 cut(s) 403, 483, 574
HpyAV CCTTC 3 cut(s) 296, 800, 849
HpyCH4III ACNGT 2 cut(s) 120, 442
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 6 cut(s) 175, 269, 592, 770, 794, 923
HpyF10VI GCNNNNNNNGC 2 cut(s) 263, 695
HpyF3I CTNAG 2 cut(s) 775, 814
HpySE526I ACGT 1 cut(s) 498
Hsp92II CATG 3 cut(s) 175, 361, 419
HspAI GCGC 2 cut(s) 244, 716
KspAI GTTAAC 1 cut(s) 574
Kzo9I GATC 5 cut(s) 159, 237, 454, 865, 894
LmnI GCTCC 1 cut(s) 407
Lsp1109I GCAGC 4 cut(s) 253, 345, 778, 806
LweI GCATC 4 cut(s) 253, 419, 757, 778
MabI ACCWGGT 1 cut(s) 111
MaeII ACGT 1 cut(s) 498
MaeIII GTNAC 2 cut(s) 79, 494
MalI GATC 5 cut(s) 161, 239, 456, 867, 896
MboI GATC 5 cut(s) 159, 237, 454, 865, 894
MboII GAAGA 6 cut(s) 77, 159, 272, 512, 694, 834
MfeI CAATTG 1 cut(s) 232
MflI RGATCY 2 cut(s) 454, 865
MhlI GDGCHC 1 cut(s) 440
MluCI AATT 9 cut(s) 18, 43, 65, 232, 345, 602, 759, 829, 883
MlyI GAGTC 1 cut(s) 911
MnlI CCTC 5 cut(s) 70, 133, 655, 723, 823
Mph1103I ATGCAT 1 cut(s) 177
MroXI GAANNNNTTC 1 cut(s) 285
MseI TTAA 3 cut(s) 212, 573, 701
MslI CAYNNNNRTG 2 cut(s) 201, 567
MspI CCGG 1 cut(s) 913
MspR9I CCNGG 4 cut(s) 53, 113, 863, 913
MunI CAATTG 1 cut(s) 232
MvaI CCWGG 3 cut(s) 53, 113, 863
MwoI GCNNNNNNNGC 2 cut(s) 263, 695
NciI CCSGG 1 cut(s) 913
NdeII GATC 5 cut(s) 159, 237, 454, 865, 894
NlaIII CATG 3 cut(s) 175, 361, 419
NlaIV GGNNCC 3 cut(s) 456, 811, 859
NmuCI GTSAC 1 cut(s) 494
NsiI ATGCAT 1 cut(s) 177
OliI CACNNNNGTG 1 cut(s) 567
PdmI GAANNNNTTC 1 cut(s) 285
PfeI GAWTC 1 cut(s) 276
PkrI GCNGC 4 cut(s) 268, 335, 793, 796
Ple19I CGATCG 1 cut(s) 897
PleI GAGTC 1 cut(s) 910
PpsI GAGTC 1 cut(s) 910
Psp6I CCWGG 3 cut(s) 51, 111, 861
PspFI CCCAGC 1 cut(s) 474
PspGI CCWGG 3 cut(s) 51, 111, 861
PspN4I GGNNCC 3 cut(s) 456, 811, 859
PspPI GGNCC 1 cut(s) 206
PstI CTGCAG 1 cut(s) 796
PsuI RGATCY 2 cut(s) 454, 865
PvuI CGATCG 1 cut(s) 897
RsaI GTAC 1 cut(s) 117
RsaNI GTAC 1 cut(s) 116
RseI CAYNNNNRTG 2 cut(s) 201, 567
SaqAI TTAA 3 cut(s) 212, 573, 701
SatI GCNGC 4 cut(s) 267, 334, 792, 795
Sau3AI GATC 5 cut(s) 159, 237, 454, 865, 894
Sau96I GGNCC 1 cut(s) 206
SchI GAGTC 1 cut(s) 911
ScrFI CCNGG 4 cut(s) 53, 113, 863, 913
SduI GDGCHC 1 cut(s) 440
SexAI ACCWGGT 1 cut(s) 111
SfaNI GCATC 4 cut(s) 253, 419, 757, 778
SfcI CTRYAG 2 cut(s) 441, 792
SmiMI CAYNNNNRTG 2 cut(s) 201, 567
SmlI CTYRAG 4 cut(s) 188, 248, 524, 752
SmoI CTYRAG 4 cut(s) 188, 248, 524, 752
Sse9I AATT 9 cut(s) 18, 43, 65, 232, 345, 602, 759, 829, 883
SsiI CCGC 1 cut(s) 23
StyD4I CCNGG 4 cut(s) 51, 111, 861, 911
StyI CCWWGG 1 cut(s) 846
TaaI ACNGT 2 cut(s) 120, 442
TaiI ACGT 1 cut(s) 501
TaqI TCGA 4 cut(s) 279, 639, 893, 897
TasI AATT 9 cut(s) 18, 43, 65, 232, 345, 602, 759, 829, 883
TfiI GAWTC 1 cut(s) 276
Tru1I TTAA 3 cut(s) 212, 573, 701
Tru9I TTAA 3 cut(s) 212, 573, 701
TscAI CASTG 2 cut(s) 445, 783
TseFI GTSAC 1 cut(s) 494
TseI GCWGC 4 cut(s) 266, 333, 791, 794
Tsp45I GTSAC 1 cut(s) 494
TspDTI ATGAA 4 cut(s) 17, 24, 160, 305
TspGWI ACGGA 2 cut(s) 420, 735
TspRI CASTG 2 cut(s) 445, 783
XapI RAATTY 3 cut(s) 65, 759, 829
XmiI GTMKAC 1 cut(s) 402
XmnI GAANNNNTTC 1 cut(s) 285
Zsp2I ATGCAT 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.