MD12G1125000.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0028728
Physical Location & Seq
Reverse (-)
20003521 .. 20007512
3992 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1125000.v1.1.491

Sequence Viewer

Length: 1725 bp
ATGGAGGTTTCCCTTCTCCTTCTCCTTCTCTCTCTCCTCCTCCTCCTCATCGTCCCTTCCTCTGCTCAAAATCCAGCAGGGTTTGTGAGTTTGGATTGTGGAGGTAAAGAAATTTTCACTGATGATCTGGGTGTGGAGTGGACTCCTGATCGGCTCAATTTCGGCGAGACGTCCTCTATAGCTGTTTCCAATAAGACCAGGAAGCAGTACTCGACGTTGAGGCATTTTCCGGCGGATTTGAGAAAATATTGTTACACATTGAATGTCACAAGTAGGACTAGGTACCTTCTAAGAGCGACATTCTTGTATGGGAACTTTGACAGCAATAACGTTTATCCCAAATTTGACATTTCTGTTGGGGCAACCCATTGGTCTTCGATTGTAATTTCGGATGCCAATACGATAGAGATGCGGGAGCTTATATTTCTGGCGACGAGTCCTACTGTCAGTGTATGTTTATCCAATGCTACGACTGGACAACCTTTTATATCCACTCTTGAGCTTCGACAATTCAATGGTTCCATGTACTATTCTGAGTTTGAGCAACAGTTCTATCTCAGTGTCTCTGCCAGGATTAATTTCGGTGCAGATGATGAAGCACCAGTCAGGTATCCTGATGACCCTTTCGATAGAATATGGACATCAGACTCTGTCAAGAAAGCGAATTACCTCGTCGACATTGCTGCTGGAACCAAAAAAGTGTCAACCAAGTTGCCAATTGACATTAATGGGGATGACAGGCCACCCGAAAAAGTTATGCAGACAGCTGTAGTTGGCACAAATGGAACTCTGACTTACCGGCTGAACCTGGATGGTTTTCCTGGTTTTGGATGGGCATACACCTACTTAGCAGAAATTGAAGATTTAGCTGAAGATGAGTCTAGAAAGTTTAGGCTAGTACTTCCAGGAAATACTGAACTCAGCATACCTGTTGTTAATATTGAAGAAAACGCTCACGGAAAATATCGTCTCTATGAACCAGGGTTTACAAACTTATCCCTTCCGTTTGTATTATCATTTAAATTTGCCAAAACAGCCGATTCTTCCAGGGGACCGCTCTTGAATGCAATGGAGATAAATAAATATCTGGAAATAAATGATGGTTCTCAAGATAGAGCTGTTATTTCTAGTTTTGTTTCACACTACTCATCGTCAGATTGGAATTTGGAAGGCGGTGATCCATGCCTCCCAGTTCCATGGTCTTGGGTGGAGTGTAACTCAGATCCACAACCAAGAGTAGTTCAAATAAAATTATCTAGTAAGAATATTACTGGGAATATCCCTTCAGACTTGACAAAGTTGAGCAGTTTACAAGAGTTATGGCTTGACGGGAACTCACTGACTGGTACAATACCTGATTTCACTGGATGTGTGGACTTGAAGATCATTCATCTTGAGAACAATCAGTTGACGGGCGGACTCTCCTCTTTCACGAACCTACCAAGTTTGAAGGAACTGTATGTGCAAAATAATATGTTAACCGGAACTGTGCCATCAAGTCTTCTCGATAAAGTGGTTAATATGAACTACGCTGGAAATGTTAATCTGCGGAAAGGGAAAACAAGCGGGAGCCGCAATAACATTATTATTGGTACATCAGTTGGGGCTGCTGTGTTGGTCATAGCTACTATTGTATCTTGCGTACTTTTACGCAAGCGAAGGAAGAAAGTTTACAAGCATGACCAACTTGGGAATCAACCTTGCGCAAGGAATACATTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

575

Amino Acids

63.68

Weight (kDa)

5.83

Isoelectric Point (pI)

34.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 31 - 360 3.1e-74 Malectin-like domain
LRR_8 PF13855 436 - 493 5.7e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028728)

Species Orthologous Gene IDs
malus_domestica MD12G1125000.v1.1
rosa_multiflora Rmu_ssc0000444.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 173
Acc16I TGCGCA 1 cut(s) 1705
Acc65I GGTACC 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 282
AccBSI CCGCTC 1 cut(s) 1057
AccI GTMKAC 1 cut(s) 675
AciI CCGC 8 cut(s) 233, 412, 1055, 1173, 1416, 1549, 1566, 1573
AclI AACGTT 1 cut(s) 330
AclWI GGATC 2 cut(s) 1172, 1217
AcsI RAATTY 4 cut(s) 111, 341, 1022, 1162
AcuI CTGAAG 2 cut(s) 891, 1269
AcyI GRCGYC 1 cut(s) 170
AfaI GTAC 7 cut(s) 209, 284, 527, 900, 1348, 1594, 1644
AfiI CCNNNNNNNGG 1 cut(s) 827
AgsI TTSAA 8 cut(s) 262, 514, 860, 944, 1063, 1244, 1381, 1450
AjnI CCWGG 7 cut(s) 197, 569, 807, 820, 904, 979, 1046
AluBI AGCT 7 cut(s) 182, 418, 502, 767, 869, 1118, 1625
AluI AGCT 7 cut(s) 182, 418, 502, 767, 869, 1118, 1625
Alw26I GTCTC 3 cut(s) 161, 568, 974
AlwI GGATC 2 cut(s) 1172, 1217
AlwNI CAGNNNCTG 1 cut(s) 650
AoxI GGCC 1 cut(s) 740
ApeKI GCWGC 2 cut(s) 683, 1607
ApoI RAATTY 4 cut(s) 111, 341, 1022, 1162
AseI ATTAAT 2 cut(s) 576, 726
Asp700I GAANNNNTTC 1 cut(s) 1715
Asp718I GGTACC 1 cut(s) 282
AspLEI GCGC 1 cut(s) 1706
AspS9I GGNCC 1 cut(s) 1052
AsuHPI GGTGA 1 cut(s) 1187
AvaII GGWCC 1 cut(s) 1052
BaeI ACNNNNGTAYC 4 cut(s) 1584, 1617, 1617, 1650
BanI GGYRCC 1 cut(s) 282
BarI GAAGNNNNNNTAC 2 cut(s) 1655, 1687
BbsI GAAGAC 2 cut(s) 366, 1493
BbvI GCAGC 2 cut(s) 670, 1594
BccI CCATC 4 cut(s) 806, 825, 1094, 1501
BcgI CGANNNNNNTGC 4 cut(s) 391, 425, 665, 699
BciT130I CCWGG 7 cut(s) 199, 571, 809, 822, 906, 981, 1048
BciVI GTATCC 1 cut(s) 621
BcoDI GTCTC 3 cut(s) 161, 568, 974
BfaI CTAG 5 cut(s) 279, 882, 896, 1128, 1257
BfmI CTRYAG 2 cut(s) 177, 768
BfuI GTATCC 1 cut(s) 621
BisI GCNGC 3 cut(s) 684, 1573, 1608
BlsI GCNGC 3 cut(s) 685, 1574, 1609
BmcAI AGTACT 2 cut(s) 209, 900
Bme1390I CCNGG 7 cut(s) 199, 571, 809, 822, 906, 981, 1048
Bme18I GGWCC 1 cut(s) 1052
BmgT120I GGNCC 1 cut(s) 1052
BmiI GGNNCC 5 cut(s) 284, 520, 691, 1053, 1571
BmrFI CCNGG 7 cut(s) 199, 571, 809, 822, 906, 981, 1048
BmrI ACTGGG 2 cut(s) 1184, 1281
BmsI GCATC 2 cut(s) 382, 399
BmuI ACTGGG 2 cut(s) 1184, 1281
BpiI GAAGAC 2 cut(s) 366, 1493
BplI GAGNNNNNCTC 4 cut(s) 158, 190, 1202, 1234
BpuEI CTTGAG 3 cut(s) 518, 1092, 1415
BsaHI GRCGYC 1 cut(s) 170
BsaJI CCNNGG 3 cut(s) 980, 1047, 1196
BsaWI WCCGGW 1 cut(s) 1481
Bsc4I CCNNNNNNNGG 1 cut(s) 827
Bse118I RCCGGY 1 cut(s) 798
Bse1I ACTGG 6 cut(s) 478, 602, 1190, 1276, 1348, 1369
Bse3DI GCAATG 2 cut(s) 678, 1074
BseBI CCWGG 7 cut(s) 199, 571, 809, 822, 906, 981, 1048
BseDI CCNNGG 3 cut(s) 980, 1047, 1196
BseGI GGATG 5 cut(s) 397, 739, 817, 836, 1373
BseLI CCNNNNNNNGG 1 cut(s) 827
BseMI GCAATG 2 cut(s) 678, 1074
BseMII CTCAG 4 cut(s) 525, 571, 934, 1233
BseNI ACTGG 6 cut(s) 478, 602, 1190, 1276, 1348, 1369
BseRI GAGGAG 5 cut(s) 26, 29, 32, 35, 1414
BseXI GCAGC 2 cut(s) 670, 1594
BsgI GTGCAG 1 cut(s) 606
BshFI GGCC 1 cut(s) 742
BshNI GGYRCC 1 cut(s) 282
BsiSI CCGG 3 cut(s) 230, 799, 1482
BslFI GGGAC 2 cut(s) 38, 1065
BslI CCNNNNNNNGG 1 cut(s) 827
BsmAI GTCTC 3 cut(s) 161, 568, 974
BsmBI CGTCTC 2 cut(s) 161, 974
BsmFI GGGAC 2 cut(s) 38, 1065
BsmI GAATGC 1 cut(s) 1069
BsnI GGCC 1 cut(s) 742
Bsp143I GATC 5 cut(s) 124, 148, 1177, 1222, 1383
Bsp19I CCATGG 1 cut(s) 1196
BspACI CCGC 8 cut(s) 233, 412, 1055, 1173, 1416, 1549, 1566, 1573
BspANI GGCC 1 cut(s) 742
BspCNI CTCAG 4 cut(s) 526, 570, 933, 1232
BspLI GGNNCC 5 cut(s) 284, 520, 691, 1053, 1571
BspPI GGATC 2 cut(s) 1172, 1217
BspT107I GGYRCC 1 cut(s) 282
BsrBI CCGCTC 1 cut(s) 1057
BsrDI GCAATG 2 cut(s) 678, 1074
BsrFI RCCGGY 1 cut(s) 798
BsrI ACTGG 6 cut(s) 478, 602, 1190, 1276, 1348, 1369
BssAI RCCGGY 1 cut(s) 798
BssECI CCNNGG 3 cut(s) 980, 1047, 1196
BssMI GATC 5 cut(s) 124, 148, 1177, 1222, 1383
BssNI GRCGYC 1 cut(s) 170
BssT1I CCWWGG 1 cut(s) 1196
Bst2UI CCWGG 7 cut(s) 199, 571, 809, 822, 906, 981, 1048
Bst4CI ACNGT 4 cut(s) 445, 549, 1458, 1489
BstACI GRCGYC 1 cut(s) 170
BstC8I GCNNGC 1 cut(s) 1655
BstDEI CTNAG 6 cut(s) 290, 534, 557, 847, 920, 1219
BstDSI CCRYGG 1 cut(s) 1196
BstF5I GGATG 5 cut(s) 397, 739, 817, 836, 1373
BstHHI GCGC 1 cut(s) 1706
BstKTI GATC 5 cut(s) 127, 151, 1180, 1225, 1386
BstMAI GTCTC 3 cut(s) 161, 568, 974
BstMBI GATC 5 cut(s) 124, 148, 1177, 1222, 1383
BstMWI GCNNNNNNNGC 2 cut(s) 1034, 1572
BstNI CCWGG 7 cut(s) 199, 571, 809, 822, 906, 981, 1048
BstSCI CCNGG 7 cut(s) 197, 569, 807, 820, 904, 979, 1046
BstSFI CTRYAG 2 cut(s) 177, 768
BstV1I GCAGC 2 cut(s) 670, 1594
BstV2I GAAGAC 2 cut(s) 366, 1493
BstX2I RGATCY 1 cut(s) 1222
BstXI CCANNNNNNTGG 2 cut(s) 1197, 1203
BstYI RGATCY 1 cut(s) 1222
BsuI GTATCC 1 cut(s) 621
BsuRI GGCC 1 cut(s) 742
BtgI CCRYGG 1 cut(s) 1196
BtsCI GGATG 5 cut(s) 397, 739, 817, 836, 1373
BtsIMutI CAGTG 5 cut(s) 117, 454, 565, 1337, 1362
Cac8I GCNNGC 1 cut(s) 1655
CaiI CAGNNNCTG 1 cut(s) 650
CfoI GCGC 1 cut(s) 1706
Cfr10I RCCGGY 1 cut(s) 798
Cfr13I GGNCC 1 cut(s) 1052
Csp6I GTAC 7 cut(s) 208, 283, 526, 899, 1347, 1593, 1643
CviAII CATG 4 cut(s) 523, 1182, 1197, 1679
CviQI GTAC 7 cut(s) 208, 283, 526, 899, 1347, 1593, 1643
DdeI CTNAG 6 cut(s) 290, 534, 557, 847, 920, 1219
DpnI GATC 5 cut(s) 126, 150, 1179, 1224, 1385
DpnII GATC 5 cut(s) 124, 148, 1177, 1222, 1383
DraI TTTAAA 1 cut(s) 1021
EciI GGCGGA 2 cut(s) 248, 1431
Eco130I CCWWGG 1 cut(s) 1196
Eco47I GGWCC 1 cut(s) 1052
Eco57I CTGAAG 2 cut(s) 891, 1269
EcoRII CCWGG 7 cut(s) 197, 569, 807, 820, 904, 979, 1046
EcoT14I CCWWGG 1 cut(s) 1196
ErhI CCWWGG 1 cut(s) 1196
Esp3I CGTCTC 2 cut(s) 161, 974
FaeI CATG 4 cut(s) 526, 1185, 1200, 1682
FaqI GGGAC 2 cut(s) 38, 1065
FatI CATG 4 cut(s) 522, 1181, 1196, 1678
FauI CCCGC 2 cut(s) 405, 1559
FblI GTMKAC 1 cut(s) 675
Fnu4HI GCNGC 3 cut(s) 684, 1573, 1608
FokI GGATG 5 cut(s) 404, 746, 824, 843, 1380
Fsp4HI GCNGC 3 cut(s) 684, 1573, 1608
FspBI CTAG 5 cut(s) 279, 882, 896, 1128, 1257
FspI TGCGCA 1 cut(s) 1705
GlaI GCGC 1 cut(s) 1705
GluI GCNGC 3 cut(s) 684, 1573, 1608
HaeIII GGCC 1 cut(s) 742
HapII CCGG 3 cut(s) 230, 799, 1482
HhaI GCGC 1 cut(s) 1706
Hin1I GRCGYC 1 cut(s) 170
Hin1II CATG 4 cut(s) 526, 1185, 1200, 1682
Hin6I GCGC 1 cut(s) 1704
HinP1I GCGC 1 cut(s) 1704
HincII GTYRAC 4 cut(s) 676, 705, 1410, 1479
HindII GTYRAC 4 cut(s) 676, 705, 1410, 1479
HinfI GANTC 7 cut(s) 142, 436, 647, 878, 1040, 1419, 1693
HpaI GTTAAC 1 cut(s) 1479
HpaII CCGG 3 cut(s) 230, 799, 1482
HphI GGTGA 1 cut(s) 1187
Hpy166II GTNNAC 9 cut(s) 141, 676, 705, 987, 1310, 1375, 1410, 1479, 1672
Hpy188I TCNGA 7 cut(s) 391, 535, 646, 792, 1156, 1222, 1288
Hpy8I GTNNAC 9 cut(s) 141, 676, 705, 987, 1310, 1375, 1410, 1479, 1672
Hpy99I CGWCG 3 cut(s) 217, 436, 677
HpyCH4III ACNGT 4 cut(s) 445, 549, 1458, 1489
HpyCH4IV ACGT 3 cut(s) 170, 215, 330
HpyCH4V TGCA 4 cut(s) 587, 760, 1067, 1465
HpyF10VI GCNNNNNNNGC 2 cut(s) 1034, 1572
HpyF3I CTNAG 6 cut(s) 290, 534, 557, 847, 920, 1219
HpySE526I ACGT 3 cut(s) 170, 215, 330
Hsp92I GRCGYC 1 cut(s) 170
Hsp92II CATG 4 cut(s) 526, 1185, 1200, 1682
HspAI GCGC 1 cut(s) 1704
KpnI GGTACC 1 cut(s) 286
KspAI GTTAAC 1 cut(s) 1479
Kzo9I GATC 5 cut(s) 124, 148, 1177, 1222, 1383
LmnI GCTCC 2 cut(s) 415, 1569
Lsp1109I GCAGC 2 cut(s) 670, 1594
LweI GCATC 2 cut(s) 382, 399
MaeI CTAG 5 cut(s) 279, 882, 896, 1128, 1257
MaeII ACGT 3 cut(s) 170, 215, 330
MaeIII GTNAC 3 cut(s) 251, 265, 1214
MalI GATC 5 cut(s) 126, 150, 1179, 1224, 1385
MbiI CCGCTC 1 cut(s) 1057
MboI GATC 5 cut(s) 124, 148, 1177, 1222, 1383
MboII GAAGA 9 cut(s) 366, 872, 884, 956, 1035, 1393, 1493, 1675, 1711
MfeI CAATTG 1 cut(s) 717
MflI RGATCY 1 cut(s) 1222
MlyI GAGTC 5 cut(s) 136, 445, 641, 887, 1413
MroXI GAANNNNTTC 1 cut(s) 1715
MseI TTAA 7 cut(s) 576, 726, 936, 1020, 1478, 1518, 1542
MspA1I CMGCKG 1 cut(s) 767
MspI CCGG 3 cut(s) 230, 799, 1482
MspR9I CCNGG 7 cut(s) 199, 571, 809, 822, 906, 981, 1048
MunI CAATTG 1 cut(s) 717
Mva1269I GAATGC 1 cut(s) 1069
MvaI CCWGG 7 cut(s) 199, 571, 809, 822, 906, 981, 1048
MwoI GCNNNNNNNGC 2 cut(s) 1034, 1572
NcoI CCATGG 1 cut(s) 1196
NdeII GATC 5 cut(s) 124, 148, 1177, 1222, 1383
NlaIII CATG 4 cut(s) 526, 1185, 1200, 1682
NlaIV GGNNCC 5 cut(s) 284, 520, 691, 1053, 1571
NmuCI GTSAC 1 cut(s) 265
NsbI TGCGCA 1 cut(s) 1705
PctI GAATGC 1 cut(s) 1069
PdmI GAANNNNTTC 1 cut(s) 1715
PfeI GAWTC 2 cut(s) 1040, 1693
PflFI GACNNNGTC 1 cut(s) 650
PfoI TCCNGGA 1 cut(s) 904
PkrI GCNGC 3 cut(s) 685, 1574, 1609
PleI GAGTC 5 cut(s) 136, 444, 641, 886, 1413
PpsI GAGTC 5 cut(s) 136, 444, 641, 886, 1413
PshBI ATTAAT 2 cut(s) 576, 726
Psp1406I AACGTT 1 cut(s) 330
Psp6I CCWGG 7 cut(s) 197, 569, 807, 820, 904, 979, 1046
PspGI CCWGG 7 cut(s) 197, 569, 807, 820, 904, 979, 1046
PspN4I GGNNCC 5 cut(s) 284, 520, 691, 1053, 1571
PspPI GGNCC 1 cut(s) 1052
PsrI GAACNNNNNNTAC 2 cut(s) 909, 941
PstNI CAGNNNCTG 1 cut(s) 650
PsuI RGATCY 1 cut(s) 1222
PsyI GACNNNGTC 1 cut(s) 650
PvuII CAGCTG 1 cut(s) 767
RsaI GTAC 7 cut(s) 209, 284, 527, 900, 1348, 1594, 1644
RsaNI GTAC 7 cut(s) 208, 283, 526, 899, 1347, 1593, 1643
SalI GTCGAC 1 cut(s) 674
SaqAI TTAA 7 cut(s) 576, 726, 936, 1020, 1478, 1518, 1542
SatI GCNGC 3 cut(s) 684, 1573, 1608
Sau3AI GATC 5 cut(s) 124, 148, 1177, 1222, 1383
Sau96I GGNCC 1 cut(s) 1052
ScaI AGTACT 2 cut(s) 209, 900
SchI GAGTC 5 cut(s) 136, 445, 641, 887, 1413
ScrFI CCNGG 7 cut(s) 199, 571, 809, 822, 906, 981, 1048
SfaNI GCATC 2 cut(s) 382, 399
SfcI CTRYAG 2 cut(s) 177, 768
SinI GGWCC 1 cut(s) 1052
SmiI ATTTAAAT 1 cut(s) 1021
SmlI CTYRAG 3 cut(s) 497, 1107, 1394
SmoI CTYRAG 3 cut(s) 497, 1107, 1394
SsiI CCGC 8 cut(s) 233, 412, 1055, 1173, 1416, 1549, 1566, 1573
SspI AATATT 3 cut(s) 248, 940, 1267
SspMI CTAG 5 cut(s) 279, 882, 896, 1128, 1257
StyD4I CCNGG 7 cut(s) 197, 569, 807, 820, 904, 979, 1046
StyI CCWWGG 1 cut(s) 1196
SwaI ATTTAAAT 1 cut(s) 1021
TaaI ACNGT 4 cut(s) 445, 549, 1458, 1489
TaiI ACGT 3 cut(s) 173, 218, 333
TaqI TCGA 6 cut(s) 212, 377, 505, 627, 675, 1506
TatI WGTACW 3 cut(s) 207, 525, 898
TauI GCSGC 1 cut(s) 1575
TfiI GAWTC 2 cut(s) 1040, 1693
Tru1I TTAA 7 cut(s) 576, 726, 936, 1020, 1478, 1518, 1542
Tru9I TTAA 7 cut(s) 576, 726, 936, 1020, 1478, 1518, 1542
TscAI CASTG 5 cut(s) 124, 454, 565, 1344, 1369
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 2 cut(s) 683, 1607
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 4 cut(s) 609, 990, 1379, 1538
TspGWI ACGGA 2 cut(s) 972, 993
TspRI CASTG 5 cut(s) 124, 454, 565, 1344, 1369
Tth111I GACNNNGTC 1 cut(s) 650
VpaK11BI GGWCC 1 cut(s) 1052
VspI ATTAAT 2 cut(s) 576, 726
XapI RAATTY 4 cut(s) 111, 341, 1022, 1162
XbaI TCTAGA 1 cut(s) 881
XmiI GTMKAC 1 cut(s) 675
XmnI GAANNNNTTC 1 cut(s) 1715
XspI CTAG 5 cut(s) 279, 882, 896, 1128, 1257
ZraI GACGTC 1 cut(s) 171
ZrmI AGTACT 2 cut(s) 209, 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.