MD12G1175900.v1.1

EG45-like domain containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0024425
Physical Location & Seq
Reverse (-)
25627960 .. 25628441
482 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1175900.v1.1.491

Sequence Viewer

Length: 249 bp
ATGTACCTTTTTGCGGCGGTGAGTGAAGGGTTGTGGGACGGTGGTTCTGCGTGTGGAAGGCTCTATAAAATTAGGTGCCTGAGTGGACGTAATATGCCCTGCAAGGGTGGTGCTACTGTGGAGGTGAAGGTGGTGGACCTTTGCCGTCAATCTCCGTGCCCTTCTACCATTGCCATGTCAACCAATGCTTTTGCAGCCATCTCAAACTCATCTTCTACACAAATCAACATCGAATATCTTCAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

83

Amino Acids

8.75

Weight (kDa)

8.35

Isoelectric Point (pI)

67.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 5 - 79 1.6e-11 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024425)

Species Orthologous Gene IDs
malus_domestica MD12G1175200.v1.1 MD12G1175900.v1.1
pyrus_communis pycom12g16630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 75
AciI CCGC 2 cut(s) 14, 17
AcuI CTGAAG 1 cut(s) 224
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 13, 104
ApeKI GCWGC 1 cut(s) 194
Asp700I GAANNNNTTC 1 cut(s) 237
AspS9I GGNCC 1 cut(s) 136
AsuHPI GGTGA 2 cut(s) 31, 136
AvaII GGWCC 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 161
BanI GGYRCC 1 cut(s) 75
BbvI GCAGC 1 cut(s) 206
BccI CCATC 1 cut(s) 206
BceAI ACGGC 1 cut(s) 129
BisI GCNGC 2 cut(s) 15, 195
BlsI GCNGC 2 cut(s) 16, 196
Bme18I GGWCC 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 1 cut(s) 77
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 104
Bse3DI GCAATG 1 cut(s) 168
BseLI CCNNNNNNNGG 2 cut(s) 13, 104
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 1 cut(s) 71
BseSI GKGCMC 1 cut(s) 161
BseXI GCAGC 1 cut(s) 206
BshNI GGYRCC 1 cut(s) 75
BslFI GGGAC 1 cut(s) 50
BslI CCNNNNNNNGG 2 cut(s) 13, 104
BsmFI GGGAC 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 161
BspACI CCGC 2 cut(s) 14, 17
BspCNI CTCAG 1 cut(s) 72
BspLI GGNNCC 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 75
BsrDI GCAATG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 41, 118
BstDEI CTNAG 1 cut(s) 80
BstMWI GCNNNNNNNGC 1 cut(s) 194
BstSLI GKGCMC 1 cut(s) 161
BstV1I GCAGC 1 cut(s) 206
Cfr13I GGNCC 1 cut(s) 136
Csp6I GTAC 1 cut(s) 4
CviAII CATG 1 cut(s) 175
CviJI RGCY 2 cut(s) 61, 197
CviKI_1 RGCY 2 cut(s) 61, 197
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 1 cut(s) 80
Eco47I GGWCC 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 224
FaeI CATG 1 cut(s) 178
FaiI YATR 4 cut(s) 66, 95, 176, 247
FaqI GGGAC 1 cut(s) 50
FatI CATG 1 cut(s) 174
Fnu4HI GCNGC 2 cut(s) 15, 195
Fsp4HI GCNGC 2 cut(s) 15, 195
GluI GCNGC 2 cut(s) 15, 195
Hin1II CATG 1 cut(s) 178
HincII GTYRAC 1 cut(s) 180
HindII GTYRAC 1 cut(s) 180
HphI GGTGA 2 cut(s) 31, 136
Hpy166II GTNNAC 3 cut(s) 86, 136, 180
Hpy188I TCNGA 1 cut(s) 243
Hpy8I GTNNAC 3 cut(s) 86, 136, 180
HpyAV CCTTC 4 cut(s) 20, 51, 121, 171
HpyCH4III ACNGT 2 cut(s) 41, 118
HpyCH4IV ACGT 1 cut(s) 88
HpyCH4V TGCA 2 cut(s) 102, 194
HpyF10VI GCNNNNNNNGC 1 cut(s) 194
HpyF3I CTNAG 1 cut(s) 80
HpySE526I ACGT 1 cut(s) 88
Hsp92II CATG 1 cut(s) 178
LpnPI CCDG 2 cut(s) 92, 112
Lsp1109I GCAGC 1 cut(s) 206
MaeII ACGT 1 cut(s) 88
MboII GAAGA 2 cut(s) 204, 230
MhlI GDGCHC 1 cut(s) 161
MluCI AATT 1 cut(s) 69
MnlI CCTC 1 cut(s) 115
MroXI GAANNNNTTC 1 cut(s) 237
MslI CAYNNNNRTG 1 cut(s) 173
MwoI GCNNNNNNNGC 1 cut(s) 194
NlaIII CATG 1 cut(s) 178
NlaIV GGNNCC 1 cut(s) 77
PdmI GAANNNNTTC 1 cut(s) 237
PkrI GCNGC 2 cut(s) 16, 196
PspN4I GGNNCC 1 cut(s) 77
PspPI GGNCC 1 cut(s) 136
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 1 cut(s) 173
SatI GCNGC 2 cut(s) 15, 195
Sau96I GGNCC 1 cut(s) 136
SduI GDGCHC 1 cut(s) 161
SetI ASST 6 cut(s) 9, 77, 91, 126, 132, 141
SgeI CNNG 6 cut(s) 63, 91, 111, 115, 168, 187
SinI GGWCC 1 cut(s) 136
SmiMI CAYNNNNRTG 1 cut(s) 173
Sse9I AATT 1 cut(s) 69
SsiI CCGC 2 cut(s) 14, 17
TaaI ACNGT 2 cut(s) 41, 118
TaiI ACGT 1 cut(s) 91
TaqI TCGA 1 cut(s) 231
TasI AATT 1 cut(s) 69
TauI GCSGC 1 cut(s) 17
TseI GCWGC 1 cut(s) 194
TspGWI ACGGA 1 cut(s) 144
VpaK11BI GGWCC 1 cut(s) 136
XmnI GAANNNNTTC 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.