MD12G1202000.v1.1

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0028721
Physical Location & Seq
Forward (+)
28264912 .. 28267158
2247 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1202000.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGCGGGTTTTTAATCACCGTTGTGAAACCCCTAGCATTTGTGCTGACAAGGAATGTCGAATCATCCCATGGATCAAGAATGTTGTAAGGTGCAACGTGGAACTGCTTGATCTTCATATGATTCTACCTGACGTGAAGAAGCCATTTGAATTTCCATCTTGCATCTTTCTTTGTGGATCTTTGAGGACCTTGTTGGTGCGCATACTTCATGGAACTATCAAAGCGCCCTCCTTAGCTTGTTTGTCTAATCTCGAATGCTTGAATTTGAAATATGTAACTTTAGGTCAGGATTTCTTCAATTGGATCTCATCATGCTGCAAGTGCATTAAAGAATTAAATCTTGATCGTGTTTGTGGGGAAAGAGATATCACCATTGAAAGCACATCTTTGAAATCTTTTAGTATTGTGACTAGTGAGGATAGACTTCATCAACTCAAAATCAGTGGTGAGAAACTTGAAGATATACACATAGAGTGGAGTTCAAAGTGGTGCTGGTCATTAAGTATCTTTGCTTCGAATCTTAAGTATTTGGAATGGCCTGGGAACACGGTCGTCAACGACCAGCTGGGGATATTAGAAAAGGCTGAGATTTCTTTTGCCCCTCAAGCATATGAAGCGGAAGGGAAGGCATTTGACTTACTTTGCAGCATACAACGGGTTAAAGCTCTAACGCTAGATCAAAATACCATCATGGATTTGTTCAAGGAAGGTCATGTGTCAGTACCACCATTAGATGATGTTTCTGATTTCCGTATCAACATTGGGAGCTTTAGTGATAAACTAGTGTTGCCATTGGTTTCTCTTCTTAGGGTAATGCCTAATTTGAGTATTTTGTACCTGGCGTCTACTCCGTCATGCTTTTCATACCGTAAAAATGATTGTTCAGGTTTTAATACGAAGTACTGGAAGTCACAAAACCTTCCTTTTGTTTCTCAGCTTAAAGAGGTAACTGTAGAGCTTTCCCAATTTTCCTACGGGTCTAACCCAGTTGAGTTTACAAGGTTTATCCTTGAGCATGCTCGCAACTTGAAGAAAATGGTCATCGTTCATCCACCCCGCTTTGCAAAGGCGTCTCTCGACAAGTTGAAAAAAAGCGAGAAGACTTCCAATGCTCACATTGTCTTCTGCAGAAACAAGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

381

Amino Acids

43.42

Weight (kDa)

8.48

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 24 - 148 1.1e-08 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028721)

Species Orthologous Gene IDs
malus_domestica MD12G1202000.v1.1
pyrus_communis pycom12g18850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 200
AccI GTMKAC 1 cut(s) 845
AciI CCGC 3 cut(s) 4, 617, 1057
AclWI GGATC 3 cut(s) 80, 184, 311
AcsI RAATTY 2 cut(s) 149, 262
AcyI GRCGYC 2 cut(s) 842, 1070
AfaI GTAC 4 cut(s) 723, 836, 902, 1139
AflII CTTAAG 1 cut(s) 521
AhlI ACTAGT 2 cut(s) 410, 781
AjiI CACGTC 1 cut(s) 133
AjnI CCWGG 2 cut(s) 538, 837
AleI CACNNNNGTG 1 cut(s) 21
AluBI AGCT 6 cut(s) 236, 565, 665, 768, 937, 958
AluI AGCT 6 cut(s) 236, 565, 665, 768, 937, 958
Alw26I GTCTC 1 cut(s) 1077
AlwI GGATC 3 cut(s) 80, 184, 311
AoxI GGCC 1 cut(s) 536
ApeKI GCWGC 2 cut(s) 315, 645
ApoI RAATTY 2 cut(s) 149, 262
AspLEI GCGC 2 cut(s) 201, 226
AspS9I GGNCC 1 cut(s) 186
AsuHPI GGTGA 3 cut(s) 8, 361, 458
AsuII TTCGAA 1 cut(s) 515
AvaII GGWCC 1 cut(s) 186
BarI GAAGNNNNNNTAC 2 cut(s) 496, 528
BbsI GAAGAC 2 cut(s) 1106, 1114
BbvI GCAGC 2 cut(s) 302, 657
BccI CCATC 2 cut(s) 163, 695
BciT130I CCWGG 2 cut(s) 540, 839
BcoDI GTCTC 1 cut(s) 1077
BcuI ACTAGT 2 cut(s) 410, 781
BfaI CTAG 4 cut(s) 33, 411, 674, 782
BfmI CTRYAG 2 cut(s) 951, 1126
BfoI RGCGCY 1 cut(s) 227
BfrI CTTAAG 1 cut(s) 521
BisI GCNGC 2 cut(s) 316, 646
BlsI GCNGC 2 cut(s) 317, 647
BmcAI AGTACT 2 cut(s) 902, 1139
Bme1390I CCNGG 2 cut(s) 540, 839
Bme18I GGWCC 1 cut(s) 186
BmgBI CACGTC 1 cut(s) 133
BmgT120I GGNCC 1 cut(s) 186
BmrFI CCNGG 2 cut(s) 540, 839
BmrI ACTGGG 1 cut(s) 980
BmsI GCATC 1 cut(s) 171
BmuI ACTGGG 1 cut(s) 980
BpiI GAAGAC 2 cut(s) 1106, 1114
Bpu10I CCTNAGC 1 cut(s) 232
Bpu14I TTCGAA 1 cut(s) 515
BpuEI CTTGAG 2 cut(s) 588, 1031
BsaHI GRCGYC 2 cut(s) 842, 1070
BsaJI CCNNGG 2 cut(s) 68, 539
BsaXI ACNNNNNCTCC 2 cut(s) 757, 787
Bse1I ACTGG 2 cut(s) 908, 986
BseBI CCWGG 2 cut(s) 540, 839
BseDI CCNNGG 2 cut(s) 68, 539
BseGI GGATG 2 cut(s) 63, 1048
BseMII CTCAG 2 cut(s) 576, 947
BseNI ACTGG 2 cut(s) 908, 986
BseXI GCAGC 2 cut(s) 302, 657
BseYI CCCAGC 1 cut(s) 565
Bsh1285I CGRYCG 1 cut(s) 552
BshFI GGCC 1 cut(s) 538
BsiEI CGRYCG 1 cut(s) 552
BsmAI GTCTC 1 cut(s) 1077
BsmBI CGTCTC 1 cut(s) 1077
BsmI GAATGC 1 cut(s) 260
BsnI GGCC 1 cut(s) 538
Bsp119I TTCGAA 1 cut(s) 515
Bsp143I GATC 6 cut(s) 72, 109, 176, 303, 343, 676
Bsp19I CCATGG 1 cut(s) 68
BspACI CCGC 3 cut(s) 4, 617, 1057
BspANI GGCC 1 cut(s) 538
BspCNI CTCAG 2 cut(s) 577, 946
BspMAI CTGCAG 1 cut(s) 1130
BspPI GGATC 3 cut(s) 80, 184, 311
BspT104I TTCGAA 1 cut(s) 515
BspTI CTTAAG 1 cut(s) 521
BsrI ACTGG 2 cut(s) 908, 986
BssECI CCNNGG 2 cut(s) 68, 539
BssMI GATC 6 cut(s) 72, 109, 176, 303, 343, 676
BssNI GRCGYC 2 cut(s) 842, 1070
BssT1I CCWWGG 1 cut(s) 68
Bst2UI CCWGG 2 cut(s) 540, 839
Bst4CI ACNGT 4 cut(s) 20, 550, 869, 952
Bst6I CTCTTC 1 cut(s) 807
BstACI GRCGYC 2 cut(s) 842, 1070
BstAFI CTTAAG 1 cut(s) 521
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 2 cut(s) 1017, 1021
BstDEI CTNAG 4 cut(s) 232, 585, 806, 933
BstDSI CCRYGG 1 cut(s) 68
BstF5I GGATG 2 cut(s) 63, 1048
BstH2I RGCGCY 1 cut(s) 227
BstHHI GCGC 2 cut(s) 201, 226
BstKTI GATC 6 cut(s) 75, 112, 179, 306, 346, 679
BstMAI GTCTC 1 cut(s) 1077
BstMBI GATC 6 cut(s) 72, 109, 176, 303, 343, 676
BstMCI CGRYCG 1 cut(s) 552
BstMWI GCNNNNNNNGC 3 cut(s) 321, 605, 614
BstNI CCWGG 2 cut(s) 540, 839
BstNSI RCATGY 1 cut(s) 1019
BstSCI CCNGG 2 cut(s) 538, 837
BstSFI CTRYAG 2 cut(s) 951, 1126
BstV1I GCAGC 2 cut(s) 302, 657
BstV2I GAAGAC 2 cut(s) 1106, 1114
BstX2I RGATCY 2 cut(s) 176, 303
BstYI RGATCY 2 cut(s) 176, 303
BsuRI GGCC 1 cut(s) 538
BtgI CCRYGG 1 cut(s) 68
BtrI CACGTC 1 cut(s) 133
BtsCI GGATG 2 cut(s) 63, 1048
BtsIMutI CAGTG 1 cut(s) 448
Cac8I GCNNGC 2 cut(s) 1017, 1021
CfoI GCGC 2 cut(s) 201, 226
Cfr13I GGNCC 1 cut(s) 186
CseI GACGC 2 cut(s) 831, 1059
Csp6I GTAC 4 cut(s) 722, 835, 901, 1138
CspCI CAANNNNNGTGG 2 cut(s) 424, 459
CviAII CATG 7 cut(s) 69, 209, 312, 691, 713, 855, 1016
CviJI RGCY 9 cut(s) 142, 236, 538, 565, 584, 665, 768, 937, 958
CviKI_1 RGCY 9 cut(s) 142, 236, 538, 565, 584, 665, 768, 937, 958
CviQI GTAC 4 cut(s) 722, 835, 901, 1138
DdeI CTNAG 4 cut(s) 232, 585, 806, 933
DpnI GATC 6 cut(s) 74, 111, 178, 305, 345, 678
DpnII GATC 6 cut(s) 72, 109, 176, 303, 343, 676
Eam1104I CTCTTC 1 cut(s) 807
EarI CTCTTC 1 cut(s) 807
Eco130I CCWWGG 1 cut(s) 68
Eco32I GATATC 1 cut(s) 367
Eco47I GGWCC 1 cut(s) 186
EcoO109I RGGNCCY 1 cut(s) 186
EcoRII CCWGG 2 cut(s) 538, 837
EcoRV GATATC 1 cut(s) 367
EcoT14I CCWWGG 1 cut(s) 68
ErhI CCWWGG 1 cut(s) 68
Esp3I CGTCTC 1 cut(s) 1077
FaeI CATG 7 cut(s) 72, 212, 315, 694, 716, 858, 1019
FalI AAGNNNNNCTT 2 cut(s) 370, 402
FatI CATG 7 cut(s) 68, 208, 311, 690, 712, 854, 1015
FauI CCCGC 1 cut(s) 1064
FauNDI CATATG 2 cut(s) 117, 610
FblI GTMKAC 1 cut(s) 845
Fnu4HI GCNGC 2 cut(s) 316, 646
FokI GGATG 2 cut(s) 50, 1035
Fsp4HI GCNGC 2 cut(s) 316, 646
FspAI RTGCGCAY 1 cut(s) 200
FspBI CTAG 4 cut(s) 33, 411, 674, 782
FspI TGCGCA 1 cut(s) 200
GlaI GCGC 2 cut(s) 200, 225
GluI GCNGC 2 cut(s) 316, 646
GsaI CCCAGC 1 cut(s) 569
HaeII RGCGCY 1 cut(s) 227
HaeIII GGCC 1 cut(s) 538
HgaI GACGC 2 cut(s) 831, 1059
HhaI GCGC 2 cut(s) 201, 226
Hin1I GRCGYC 2 cut(s) 842, 1070
Hin1II CATG 7 cut(s) 72, 212, 315, 694, 716, 858, 1019
Hin6I GCGC 2 cut(s) 199, 224
HinP1I GCGC 2 cut(s) 199, 224
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HinfI GANTC 3 cut(s) 60, 121, 517
HphI GGTGA 3 cut(s) 8, 361, 458
Hpy166II GTNNAC 3 cut(s) 556, 846, 996
Hpy188I TCNGA 1 cut(s) 745
Hpy188III TCNNGA 5 cut(s) 76, 251, 287, 341, 1076
Hpy8I GTNNAC 3 cut(s) 556, 846, 996
HpyAV CCTTC 4 cut(s) 614, 619, 701, 929
HpyCH4III ACNGT 4 cut(s) 20, 550, 869, 952
HpyCH4IV ACGT 2 cut(s) 96, 132
HpyCH4V TGCA 7 cut(s) 93, 162, 318, 324, 645, 1064, 1128
HpyF10VI GCNNNNNNNGC 3 cut(s) 321, 605, 614
HpyF3I CTNAG 4 cut(s) 232, 585, 806, 933
HpySE526I ACGT 2 cut(s) 96, 132
Hsp92I GRCGYC 2 cut(s) 842, 1070
Hsp92II CATG 7 cut(s) 72, 212, 315, 694, 716, 858, 1019
HspAI GCGC 2 cut(s) 199, 224
Kzo9I GATC 6 cut(s) 72, 109, 176, 303, 343, 676
LmnI GCTCC 1 cut(s) 765
Lsp1109I GCAGC 2 cut(s) 302, 657
LweI GCATC 1 cut(s) 171
MaeI CTAG 4 cut(s) 33, 411, 674, 782
MaeII ACGT 2 cut(s) 96, 132
MaeIII GTNAC 4 cut(s) 274, 406, 909, 946
MalI GATC 6 cut(s) 74, 111, 178, 305, 345, 678
MboI GATC 6 cut(s) 72, 109, 176, 303, 343, 676
MboII GAAGA 8 cut(s) 104, 148, 286, 470, 794, 1042, 1111, 1114
MfeI CAATTG 1 cut(s) 298
MflI RGATCY 2 cut(s) 176, 303
MluCI AATT 6 cut(s) 149, 262, 298, 332, 820, 965
MnlI CCTC 5 cut(s) 177, 238, 409, 612, 937
MseI TTAA 8 cut(s) 12, 327, 335, 500, 522, 660, 891, 939
MslI CAYNNNNRTG 1 cut(s) 21
MspA1I CMGCKG 1 cut(s) 565
MspCI CTTAAG 1 cut(s) 521
MspR9I CCNGG 2 cut(s) 540, 839
MunI CAATTG 1 cut(s) 298
Mva1269I GAATGC 1 cut(s) 260
MvaI CCWGG 2 cut(s) 540, 839
MwoI GCNNNNNNNGC 3 cut(s) 321, 605, 614
NcoI CCATGG 1 cut(s) 68
NdeI CATATG 2 cut(s) 117, 610
NdeII GATC 6 cut(s) 72, 109, 176, 303, 343, 676
NlaIII CATG 7 cut(s) 72, 212, 315, 694, 716, 858, 1019
NmuCI GTSAC 2 cut(s) 406, 909
NsbI TGCGCA 1 cut(s) 200
NspI RCATGY 1 cut(s) 1019
NspV TTCGAA 1 cut(s) 515
OliI CACNNNNGTG 1 cut(s) 21
PaeI GCATGC 1 cut(s) 1019
PctI GAATGC 1 cut(s) 260
PfeI GAWTC 3 cut(s) 60, 121, 517
PkrI GCNGC 2 cut(s) 317, 647
PpuMI RGGWCCY 1 cut(s) 186
Psp5II RGGWCCY 1 cut(s) 186
Psp6I CCWGG 2 cut(s) 538, 837
PspFI CCCAGC 1 cut(s) 565
PspGI CCWGG 2 cut(s) 538, 837
PspPI GGNCC 1 cut(s) 186
PspPPI RGGWCCY 1 cut(s) 186
PstI CTGCAG 1 cut(s) 1130
PsuI RGATCY 2 cut(s) 176, 303
PvuII CAGCTG 1 cut(s) 565
RsaI GTAC 4 cut(s) 723, 836, 902, 1139
RsaNI GTAC 4 cut(s) 722, 835, 901, 1138
RseI CAYNNNNRTG 1 cut(s) 21
SaqAI TTAA 8 cut(s) 12, 327, 335, 500, 522, 660, 891, 939
SatI GCNGC 2 cut(s) 316, 646
Sau3AI GATC 6 cut(s) 72, 109, 176, 303, 343, 676
Sau96I GGNCC 1 cut(s) 186
ScaI AGTACT 2 cut(s) 902, 1139
ScrFI CCNGG 2 cut(s) 540, 839
SfaNI GCATC 1 cut(s) 171
SfcI CTRYAG 2 cut(s) 951, 1126
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 1 cut(s) 186
SmiMI CAYNNNNRTG 1 cut(s) 21
SmlI CTYRAG 3 cut(s) 521, 603, 1010
SmoI CTYRAG 3 cut(s) 521, 603, 1010
SpeI ACTAGT 2 cut(s) 410, 781
SphI GCATGC 1 cut(s) 1019
Sse9I AATT 6 cut(s) 149, 262, 298, 332, 820, 965
SsiI CCGC 3 cut(s) 4, 617, 1057
SspMI CTAG 4 cut(s) 33, 411, 674, 782
StyD4I CCNGG 2 cut(s) 538, 837
StyI CCWWGG 1 cut(s) 68
TaaI ACNGT 4 cut(s) 20, 550, 869, 952
TaiI ACGT 2 cut(s) 99, 135
TaqI TCGA 4 cut(s) 58, 252, 515, 1077
TasI AATT 6 cut(s) 149, 262, 298, 332, 820, 965
TatI WGTACW 2 cut(s) 900, 1137
TfiI GAWTC 3 cut(s) 60, 121, 517
Tru1I TTAA 8 cut(s) 12, 327, 335, 500, 522, 660, 891, 939
Tru9I TTAA 8 cut(s) 12, 327, 335, 500, 522, 660, 891, 939
TscAI CASTG 1 cut(s) 448
TseFI GTSAC 2 cut(s) 406, 909
TseI GCWGC 2 cut(s) 315, 645
Tsp45I GTSAC 2 cut(s) 406, 909
TspDTI ATGAA 6 cut(s) 104, 197, 416, 627, 852, 1037
TspGWI ACGGA 2 cut(s) 740, 840
TspRI CASTG 1 cut(s) 448
Vha464I CTTAAG 1 cut(s) 521
VpaK11BI GGWCC 1 cut(s) 186
XapI RAATTY 2 cut(s) 149, 262
XceI RCATGY 1 cut(s) 1019
XmiI GTMKAC 1 cut(s) 845
XspI CTAG 4 cut(s) 33, 411, 674, 782
ZrmI AGTACT 2 cut(s) 902, 1139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.