pycom12g18850

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0028721
Physical Location & Seq
Forward (+)
20911698 .. 20913383
1686 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g18850.3

Sequence Viewer

Length: 1455 bp
ATGAGTAAGCGGGCGGCTGCTGCAGGTTGTGAGGGTCTCGGAGTTTATACGGAGTGTACGGCTGACAGGCTTAGTAATCTTCCAGAAGGAGTTGCTCACAACATCCTTTCCTTAGTCACATTTAGAGACCTCACTCGCTTTGGCAGTTTATCGAAACGCTGCAGGGAGCTCTATCTGTCAACCCCGGGATTGAATTTTAGCTTGTGGCAATTTGATGTATACACGTGTGATGAGTGGTCAAGGTTTTCAGATTCTGTTGATAGGTTCTTCGCCCTACGTGGGGATAGCAACATACATCACTTGCGTATTGGTATGCGGGTTCGTGATCACCGTTGTGAAACCCCTTGCGTTTGTGCTGCCAAGGAATGTCGAATCATCCCATGGATCGAGAATGTTGTAAGGTGCAAGGTGGAACTGCTTGATCTTCATATGATTCTACCTGACGTGAAGACGCCATTTGAATTTCCATCTTGCATCTTTCTTTGTGGATCTTTGAGGACTTTGTTGGTGCGCATACTTCATGGAACTATCAAGGCACCCTCCTTAGCTTGTTTGTCTAATCTCGAATGCTTGAATTTGAAATATGTAACTTTAGGTCAGGATTTCTTCAATTGGATCTCATCATCCTGCAAGTGCATTAAAGAATTAAATCTCGATCATGTTTGTGGGGAAAGAGATATCACCATTGAAAGCACATCTTTGAAATCTTTTAGTATTGTGACTAGTGAGGATAGACTTCATCAACTCAAAATCAGTGGTGAGAAACTTGAAGATATACACATAGAGTGGAGTTCAAAGTGGTGCTGGTCATTAAGTATCTTTGCTTCAAATCTTAAGTATTTGGAATGGCCTGGGAACATGGTCGTCAACGACCAGCTGGGGATATTAGAAAAGGCTGAGGTTTCTTTTGCCCCTCAAGCATATGAAGCGGAGGCGAAGGCATTTGACTTACTTTGCAGCATACAACGGGTTAAAGCTCTAACGCTAGATCAAAATACCATCATGGCTTTGTTCAAGGAAGGTCATGTGTCAGTACCACCATTAGATGATGTTTCTGATTTTCGTATCAACATTGGGAGCTTTAGTGATAAACTAGTGTTGCCATTGGTTTCTCTTCTTAGGGTAATGCCTAATTTGAGTATTTTGTACCTGGCGTCTTCTCCGTCATGCTTTTCATACCGTAAAAATGATTGTTCAGGTTTTAATACGAAGTACTGGAAGTCACAAAACCTTCCTTTTGTTTCTCAGCTTAAAGAGGTAACTGTAGAGCTTTCCCAATTTTCCTATGGTTCTAACCCAGTTGAGTTTACAAGGTTTATCCTTGAGCATGCTCGCAACTTGAAGAAAATGGTCATAGTTCATCCACCCCGCTTTGCGAAGGCGTCTCTCAACAAGTTGAGAAAAAGCGAGAAGACTTCCAATGCTCACGTTGTCTTCTGCAGAAACAAGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

485

Amino Acids

55.04

Weight (kDa)

8.39

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028721)

Species Orthologous Gene IDs
malus_domestica MD12G1202000.v1.1
pyrus_communis pycom12g18850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 512
Acc36I ACCTGC 1 cut(s) 14
AccB1I GGYRCC 1 cut(s) 535
AccI GTMKAC 1 cut(s) 219
AciI CCGC 5 cut(s) 10, 14, 316, 929, 1369
AclWI GGATC 3 cut(s) 392, 496, 623
AcsI RAATTY 3 cut(s) 193, 461, 574
AcvI CACGTG 1 cut(s) 225
AcyI GRCGYC 3 cut(s) 452, 1154, 1382
AfaI GTAC 5 cut(s) 58, 1035, 1148, 1214, 1451
AfiI CCNNNNNNNGG 2 cut(s) 279, 280
AflII CTTAAG 1 cut(s) 833
AflIII ACRYGT 2 cut(s) 222, 224
AhlI ACTAGT 2 cut(s) 722, 1093
AjiI CACGTC 1 cut(s) 445
AjnI CCWGG 2 cut(s) 850, 1149
AleI CACNNNNGTG 1 cut(s) 333
AluBI AGCT 8 cut(s) 169, 201, 548, 877, 977, 1080, 1249, 1270
AluI AGCT 8 cut(s) 169, 201, 548, 877, 977, 1080, 1249, 1270
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 3 cut(s) 41, 120, 1389
AlwI GGATC 3 cut(s) 392, 496, 623
AlwNI CAGNNNCTG 1 cut(s) 254
Ama87I CYCGRG 1 cut(s) 184
AoxI GGCC 1 cut(s) 848
ApeKI GCWGC 5 cut(s) 17, 20, 159, 356, 957
ApoI RAATTY 3 cut(s) 193, 461, 574
AspLEI GCGC 1 cut(s) 513
AsuC2I CCSGG 2 cut(s) 185, 186
AsuHPI GGTGA 3 cut(s) 320, 673, 770
AvaI CYCGRG 1 cut(s) 184
BanI GGYRCC 1 cut(s) 535
BanII GRGCYC 1 cut(s) 171
BarI GAAGNNNNNNTAC 2 cut(s) 808, 840
BbrPI CACGTG 1 cut(s) 225
BbsI GAAGAC 4 cut(s) 455, 1149, 1418, 1426
Bbv12I GWGCWC 1 cut(s) 171
BbvCI CCTCAGC 1 cut(s) 897
BbvI GCAGC 5 cut(s) 4, 7, 146, 343, 969
BccI CCATC 2 cut(s) 475, 1007
BceAI ACGGC 1 cut(s) 75
BciT130I CCWGG 2 cut(s) 852, 1151
BclI TGATCA 1 cut(s) 325
BcnI CCSGG 2 cut(s) 185, 186
BcoDI GTCTC 3 cut(s) 41, 120, 1389
BcuI ACTAGT 2 cut(s) 722, 1093
BfaI CTAG 3 cut(s) 723, 986, 1094
BfmI CTRYAG 4 cut(s) 21, 160, 1263, 1438
BfrI CTTAAG 1 cut(s) 833
BfuAI ACCTGC 1 cut(s) 14
BisI GCNGC 6 cut(s) 15, 18, 21, 160, 357, 958
BlsI GCNGC 6 cut(s) 16, 19, 22, 161, 358, 959
BmcAI AGTACT 2 cut(s) 1214, 1451
Bme1390I CCNGG 4 cut(s) 185, 186, 852, 1151
BmeT110I CYCGRG 1 cut(s) 184
BmgBI CACGTC 1 cut(s) 445
BmiI GGNNCC 1 cut(s) 537
BmrFI CCNGG 4 cut(s) 185, 186, 852, 1151
BmrI ACTGGG 1 cut(s) 1292
BmsI GCATC 1 cut(s) 483
BmuI ACTGGG 1 cut(s) 1292
BpiI GAAGAC 4 cut(s) 455, 1149, 1418, 1426
Bpu10I CCTNAGC 2 cut(s) 544, 897
BpuEI CTTGAG 2 cut(s) 900, 1343
BpuMI CCSGG 2 cut(s) 185, 186
BsaAI YACGTR 2 cut(s) 225, 278
BsaHI GRCGYC 3 cut(s) 452, 1154, 1382
BsaI GGTCTC 2 cut(s) 41, 120
BsaJI CCNNGG 5 cut(s) 183, 184, 360, 380, 851
BsaXI ACNNNNNCTCC 2 cut(s) 1069, 1099
Bsc4I CCNNNNNNNGG 2 cut(s) 279, 280
Bse1I ACTGG 2 cut(s) 1220, 1298
BseBI CCWGG 2 cut(s) 852, 1151
BseDI CCNNGG 5 cut(s) 183, 184, 360, 380, 851
BseGI GGATG 4 cut(s) 102, 375, 623, 1360
BseLI CCNNNNNNNGG 2 cut(s) 279, 280
BseMII CTCAG 2 cut(s) 888, 1259
BseNI ACTGG 2 cut(s) 1220, 1298
BseXI GCAGC 5 cut(s) 4, 7, 146, 343, 969
BseYI CCCAGC 1 cut(s) 877
BshFI GGCC 1 cut(s) 850
BshNI GGYRCC 1 cut(s) 535
BsiHKAI GWGCWC 1 cut(s) 171
BsiHKCI CYCGRG 1 cut(s) 184
BsiSI CCGG 1 cut(s) 185
BslI CCNNNNNNNGG 2 cut(s) 279, 280
BsmAI GTCTC 3 cut(s) 41, 120, 1389
BsmBI CGTCTC 1 cut(s) 1389
BsmI GAATGC 1 cut(s) 572
BsnI GGCC 1 cut(s) 850
Bso31I GGTCTC 2 cut(s) 41, 120
BsoBI CYCGRG 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 171
Bsp143I GATC 7 cut(s) 325, 384, 421, 488, 615, 655, 988
Bsp19I CCATGG 1 cut(s) 380
BspACI CCGC 5 cut(s) 10, 14, 316, 929, 1369
BspANI GGCC 1 cut(s) 850
BspCNI CTCAG 2 cut(s) 889, 1258
BspLI GGNNCC 1 cut(s) 537
BspMAI CTGCAG 3 cut(s) 25, 164, 1442
BspMI ACCTGC 1 cut(s) 14
BspPI GGATC 3 cut(s) 392, 496, 623
BspT107I GGYRCC 1 cut(s) 535
BspTI CTTAAG 1 cut(s) 833
BspTNI GGTCTC 2 cut(s) 41, 120
BsrI ACTGG 2 cut(s) 1220, 1298
BssECI CCNNGG 5 cut(s) 183, 184, 360, 380, 851
BssMI GATC 7 cut(s) 325, 384, 421, 488, 615, 655, 988
BssNAI GTATAC 1 cut(s) 220
BssNI GRCGYC 3 cut(s) 452, 1154, 1382
BssT1I CCWWGG 2 cut(s) 360, 380
Bst1107I GTATAC 1 cut(s) 220
Bst2UI CCWGG 2 cut(s) 852, 1151
Bst4CI ACNGT 3 cut(s) 332, 1181, 1264
Bst6I CTCTTC 1 cut(s) 1119
BstACI GRCGYC 3 cut(s) 452, 1154, 1382
BstAFI CTTAAG 1 cut(s) 833
BstBAI YACGTR 2 cut(s) 225, 278
BstC8I GCNNGC 3 cut(s) 12, 1329, 1333
BstDEI CTNAG 6 cut(s) 71, 112, 544, 897, 1118, 1245
BstDSI CCRYGG 1 cut(s) 380
BstF5I GGATG 4 cut(s) 102, 375, 623, 1360
BstHHI GCGC 1 cut(s) 513
BstKTI GATC 7 cut(s) 328, 387, 424, 491, 618, 658, 991
BstMAI GTCTC 3 cut(s) 41, 120, 1389
BstMBI GATC 7 cut(s) 325, 384, 421, 488, 615, 655, 988
BstMWI GCNNNNNNNGC 3 cut(s) 20, 917, 926
BstNI CCWGG 2 cut(s) 852, 1151
BstNSI RCATGY 1 cut(s) 1331
BstSCI CCNGG 4 cut(s) 183, 184, 850, 1149
BstSFI CTRYAG 4 cut(s) 21, 160, 1263, 1438
BstV1I GCAGC 5 cut(s) 4, 7, 146, 343, 969
BstV2I GAAGAC 4 cut(s) 455, 1149, 1418, 1426
BstX2I RGATCY 2 cut(s) 488, 615
BstYI RGATCY 2 cut(s) 488, 615
BstZ17I GTATAC 1 cut(s) 220
BsuRI GGCC 1 cut(s) 850
BtgI CCRYGG 1 cut(s) 380
BtrI CACGTC 1 cut(s) 445
BtsCI GGATG 4 cut(s) 102, 375, 623, 1360
BtsIMutI CAGTG 1 cut(s) 760
BveI ACCTGC 1 cut(s) 14
Cac8I GCNNGC 3 cut(s) 12, 1329, 1333
CaiI CAGNNNCTG 1 cut(s) 254
CfoI GCGC 1 cut(s) 513
Cfr9I CCCGGG 1 cut(s) 184
CseI GACGC 3 cut(s) 460, 1143, 1371
Csp6I GTAC 5 cut(s) 57, 1034, 1147, 1213, 1450
CspCI CAANNNNNGTGG 2 cut(s) 736, 771
CviAII CATG 8 cut(s) 381, 521, 659, 859, 1003, 1025, 1167, 1328
CviQI GTAC 5 cut(s) 57, 1034, 1147, 1213, 1450
DdeI CTNAG 6 cut(s) 71, 112, 544, 897, 1118, 1245
DpnI GATC 7 cut(s) 327, 386, 423, 490, 617, 657, 990
DpnII GATC 7 cut(s) 325, 384, 421, 488, 615, 655, 988
Eam1104I CTCTTC 1 cut(s) 1119
EarI CTCTTC 1 cut(s) 1119
Ecl136II GAGCTC 1 cut(s) 169
Eco130I CCWWGG 2 cut(s) 360, 380
Eco24I GRGCYC 1 cut(s) 171
Eco31I GGTCTC 2 cut(s) 41, 120
Eco32I GATATC 1 cut(s) 679
Eco53kI GAGCTC 1 cut(s) 169
Eco72I CACGTG 1 cut(s) 225
Eco88I CYCGRG 1 cut(s) 184
EcoICRI GAGCTC 1 cut(s) 169
EcoRII CCWGG 2 cut(s) 850, 1149
EcoRV GATATC 1 cut(s) 679
EcoT14I CCWWGG 2 cut(s) 360, 380
EcoT38I GRGCYC 1 cut(s) 171
ErhI CCWWGG 2 cut(s) 360, 380
Esp3I CGTCTC 1 cut(s) 1389
FaeI CATG 8 cut(s) 384, 524, 662, 862, 1006, 1028, 1170, 1331
FalI AAGNNNNNCTT 2 cut(s) 682, 714
FatI CATG 8 cut(s) 380, 520, 658, 858, 1002, 1024, 1166, 1327
FauI CCCGC 3 cut(s) 3, 309, 1376
FauNDI CATATG 2 cut(s) 429, 922
FbaI TGATCA 1 cut(s) 325
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 6 cut(s) 15, 18, 21, 160, 357, 958
FokI GGATG 4 cut(s) 89, 362, 610, 1347
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 6 cut(s) 15, 18, 21, 160, 357, 958
FspAI RTGCGCAY 1 cut(s) 512
FspBI CTAG 3 cut(s) 723, 986, 1094
FspI TGCGCA 1 cut(s) 512
GlaI GCGC 1 cut(s) 512
GluI GCNGC 6 cut(s) 15, 18, 21, 160, 357, 958
GsaI CCCAGC 1 cut(s) 881
HaeIII GGCC 1 cut(s) 850
HapII CCGG 1 cut(s) 185
HgaI GACGC 3 cut(s) 460, 1143, 1371
HhaI GCGC 1 cut(s) 513
Hin1I GRCGYC 3 cut(s) 452, 1154, 1382
Hin1II CATG 8 cut(s) 384, 524, 662, 862, 1006, 1028, 1170, 1331
Hin6I GCGC 1 cut(s) 511
HinP1I GCGC 1 cut(s) 511
HincII GTYRAC 2 cut(s) 180, 868
HindII GTYRAC 2 cut(s) 180, 868
HinfI GANTC 3 cut(s) 251, 372, 433
HpaII CCGG 1 cut(s) 185
HphI GGTGA 3 cut(s) 320, 673, 770
Hpy166II GTNNAC 5 cut(s) 57, 180, 220, 868, 1308
Hpy188I TCNGA 3 cut(s) 41, 250, 1057
Hpy188III TCNNGA 6 cut(s) 83, 323, 388, 563, 599, 653
Hpy8I GTNNAC 5 cut(s) 57, 180, 220, 868, 1308
HpyAV CCTTC 5 cut(s) 80, 931, 1013, 1241, 1372
HpyCH4III ACNGT 3 cut(s) 332, 1181, 1264
HpyCH4IV ACGT 4 cut(s) 224, 277, 444, 1428
HpyCH4V TGCA 8 cut(s) 23, 162, 405, 474, 630, 636, 957, 1440
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 917, 926
HpyF3I CTNAG 6 cut(s) 71, 112, 544, 897, 1118, 1245
HpySE526I ACGT 4 cut(s) 224, 277, 444, 1428
Hsp92I GRCGYC 3 cut(s) 452, 1154, 1382
Hsp92II CATG 8 cut(s) 384, 524, 662, 862, 1006, 1028, 1170, 1331
HspAI GCGC 1 cut(s) 511
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 7 cut(s) 325, 384, 421, 488, 615, 655, 988
LmnI GCTCC 2 cut(s) 166, 1077
Lsp1109I GCAGC 5 cut(s) 4, 7, 146, 343, 969
LweI GCATC 1 cut(s) 483
MaeI CTAG 3 cut(s) 723, 986, 1094
MaeII ACGT 4 cut(s) 224, 277, 444, 1428
MaeIII GTNAC 5 cut(s) 115, 586, 718, 1221, 1258
MalI GATC 7 cut(s) 327, 386, 423, 490, 617, 657, 990
MboI GATC 7 cut(s) 325, 384, 421, 488, 615, 655, 988
MfeI CAATTG 1 cut(s) 610
MflI RGATCY 2 cut(s) 488, 615
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 8 cut(s) 193, 209, 461, 574, 610, 644, 1132, 1277
MnlI CCTC 9 cut(s) 25, 140, 489, 550, 721, 892, 924, 925, 1249
MseI TTAA 7 cut(s) 639, 647, 812, 834, 972, 1203, 1251
MslI CAYNNNNRTG 2 cut(s) 333, 663
MspA1I CMGCKG 1 cut(s) 877
MspCI CTTAAG 1 cut(s) 833
MspI CCGG 1 cut(s) 185
MspR9I CCNGG 4 cut(s) 185, 186, 852, 1151
MunI CAATTG 1 cut(s) 610
Mva1269I GAATGC 1 cut(s) 572
MvaI CCWGG 2 cut(s) 852, 1151
MwoI GCNNNNNNNGC 3 cut(s) 20, 917, 926
NciI CCSGG 2 cut(s) 185, 186
NcoI CCATGG 1 cut(s) 380
NdeI CATATG 2 cut(s) 429, 922
NdeII GATC 7 cut(s) 325, 384, 421, 488, 615, 655, 988
NlaIII CATG 8 cut(s) 384, 524, 662, 862, 1006, 1028, 1170, 1331
NlaIV GGNNCC 1 cut(s) 537
NmuCI GTSAC 3 cut(s) 115, 718, 1221
NsbI TGCGCA 1 cut(s) 512
NspI RCATGY 1 cut(s) 1331
OliI CACNNNNGTG 1 cut(s) 333
PaeI GCATGC 1 cut(s) 1331
PcsI WCGNNNNNNNCGW 1 cut(s) 328
PctI GAATGC 1 cut(s) 572
PfeI GAWTC 3 cut(s) 251, 372, 433
PkrI GCNGC 6 cut(s) 16, 19, 22, 161, 358, 959
PmaCI CACGTG 1 cut(s) 225
PmlI CACGTG 1 cut(s) 225
Ppu21I YACGTR 2 cut(s) 225, 278
Psp124BI GAGCTC 1 cut(s) 171
Psp6I CCWGG 2 cut(s) 850, 1149
PspCI CACGTG 1 cut(s) 225
PspFI CCCAGC 1 cut(s) 877
PspGI CCWGG 2 cut(s) 850, 1149
PspN4I GGNNCC 1 cut(s) 537
PstI CTGCAG 3 cut(s) 25, 164, 1442
PstNI CAGNNNCTG 1 cut(s) 254
PsuI RGATCY 2 cut(s) 488, 615
PvuII CAGCTG 1 cut(s) 877
RsaI GTAC 5 cut(s) 58, 1035, 1148, 1214, 1451
RsaNI GTAC 5 cut(s) 57, 1034, 1147, 1213, 1450
RseI CAYNNNNRTG 2 cut(s) 333, 663
SacI GAGCTC 1 cut(s) 171
SaqAI TTAA 7 cut(s) 639, 647, 812, 834, 972, 1203, 1251
SatI GCNGC 6 cut(s) 15, 18, 21, 160, 357, 958
Sau3AI GATC 7 cut(s) 325, 384, 421, 488, 615, 655, 988
ScaI AGTACT 2 cut(s) 1214, 1451
ScrFI CCNGG 4 cut(s) 185, 186, 852, 1151
SduI GDGCHC 1 cut(s) 171
SfaNI GCATC 1 cut(s) 483
SfcI CTRYAG 4 cut(s) 21, 160, 1263, 1438
SmaI CCCGGG 1 cut(s) 186
SmiMI CAYNNNNRTG 2 cut(s) 333, 663
SmlI CTYRAG 3 cut(s) 833, 915, 1322
SmoI CTYRAG 3 cut(s) 833, 915, 1322
SpeI ACTAGT 2 cut(s) 722, 1093
SphI GCATGC 1 cut(s) 1331
Sse9I AATT 8 cut(s) 193, 209, 461, 574, 610, 644, 1132, 1277
SsiI CCGC 5 cut(s) 10, 14, 316, 929, 1369
SspMI CTAG 3 cut(s) 723, 986, 1094
SstI GAGCTC 1 cut(s) 171
StyD4I CCNGG 4 cut(s) 183, 184, 850, 1149
StyI CCWWGG 2 cut(s) 360, 380
TaaI ACNGT 3 cut(s) 332, 1181, 1264
TaiI ACGT 4 cut(s) 227, 280, 447, 1431
TaqI TCGA 5 cut(s) 152, 370, 387, 564, 654
TasI AATT 8 cut(s) 193, 209, 461, 574, 610, 644, 1132, 1277
TatI WGTACW 2 cut(s) 1212, 1449
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 3 cut(s) 251, 372, 433
Tru1I TTAA 7 cut(s) 639, 647, 812, 834, 972, 1203, 1251
Tru9I TTAA 7 cut(s) 639, 647, 812, 834, 972, 1203, 1251
TscAI CASTG 1 cut(s) 760
TseFI GTSAC 3 cut(s) 115, 718, 1221
TseI GCWGC 5 cut(s) 17, 20, 159, 356, 957
Tsp45I GTSAC 3 cut(s) 115, 718, 1221
TspDTI ATGAA 6 cut(s) 416, 509, 728, 939, 1164, 1349
TspGWI ACGGA 2 cut(s) 65, 1152
TspMI CCCGGG 1 cut(s) 184
TspRI CASTG 1 cut(s) 760
Vha464I CTTAAG 1 cut(s) 833
XapI RAATTY 3 cut(s) 193, 461, 574
XceI RCATGY 1 cut(s) 1331
XcmI CCANNNNNNNNNTGG 1 cut(s) 1283
XmaI CCCGGG 1 cut(s) 184
XmiI GTMKAC 1 cut(s) 219
XspI CTAG 3 cut(s) 723, 986, 1094
ZrmI AGTACT 2 cut(s) 1214, 1451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.