MD12G1217700.v1.1

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0024428
Physical Location & Seq
Reverse (-)
29505324 .. 29507936
2613 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1217700.v1.1.491

Sequence Viewer

Length: 1242 bp
ATGGAGACTTGTAGGGCAAGTACGAGTAGTGAAAAAGTTCCCAGAGTTAGTCTAGAGTTTAGGGTGGATAGGCTGAGTAGTCTTTCAGACGAAATTGCTCATGGAATCCTTTCGTTGCTTCCATTTGGAGACCTCTCTCGCTTTGGGAGTTTATCCAAACGCTGCAAGGAGCTCCATTTGTCAACTCCCAGTTTCAGATTAGATTTGTCTAAAGTAGATATATCAACTTGTGATAAGTGGTCTAGGTTCGCAAATGCTGTGGATAGGTTCTTAGACCATCGTGGGGAGAACAGAATCCTTCATTTAGGTATTAGATTTGACGTCCGCGAACATATTTTTGGAAATGAAACCCCATGCATTTGTGAGGGGGTAGGACGTCGGTTTATTTCGTGGATCGAAAATGCTTTAAGGTGCAAGGTTGAAATTCTTGACATTGATATCAGTAGGATCCATCTAGAGAATGGGGTAGGTGATATCATCATTGAGAGCTCATCACTAAAATCATTTAGTATTTCGAGCAACAGTTGGACAGTTCGTCGACTGAAAATCATCGGTCGTAAGATTGAAGATGTACGCATTGATTGGCGTTCGGAGTGGTGTACAACAGTAAGCATATTTGCTTCAGATGTTAAGAATTTGCAGTGGGATGGGATTACTGTCAACCACCAGCTCGGGACCTTAGAAAGGGCTGAGCTTTCTTTAGCCAATATAATACGTAGAGATCTATCACCTGATGTGAAGGCAGTTGACTTCCTTACCAGCGTGCACCGGGTTAAAGATTTAACTCTAAATCACAATACCATCAAGGCTTTGTTCAAGAAAGGAATACCGCCCTTAGAGAACGTTTTTGATTTCTCTATGAAAATTGGAAACTTTAGTATTGAATTGGTGTTGGCATTGGTCCCTATTCTTAGGGCAATGCCTAATTTAACTATCTTGGACGTGAAGTCTTCAACGCCGCCCCGTTGCCTTCTAAATGAATGTTCAGGTCTTACTATGGAATACTGGAGGTCAAGCAAGCTTCCTTTCCTCTCTCAGCTTAAGGAGGTAACCGTAGACCTTAATCGACACTTCTACGGGTCCAACCAAATCGAGTTCATAAGGTTTATCCTTGAGGATGCTCAAAGCCTGAGGAAAATGGTGATTATTTATCCATTACGCCTTGGAAAGGGGCATCTCAAGAAGTTGAATGAAAGCGAGAAGATTTCCAATGCTCATGTAGTCTATCGCAGGTTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

414

Amino Acids

47.31

Weight (kDa)

9.16

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024428)

Species Orthologous Gene IDs
malus_domestica MD12G1217700.v1.1 MD12G1217800.v1.1
pyrus_communis pycom12g20330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 324, 379
Acc36I ACCTGC 1 cut(s) 1221
AccI GTMKAC 2 cut(s) 538, 1056
AccII CGCG 1 cut(s) 327
AciI CCGC 3 cut(s) 325, 830, 959
AclI AACGTT 1 cut(s) 843
AclWI GGATC 3 cut(s) 401, 442, 455
AcsI RAATTY 2 cut(s) 423, 634
AcuI CTGAAG 1 cut(s) 606
AcyI GRCGYC 2 cut(s) 321, 376
AfaI GTAC 3 cut(s) 22, 573, 601
AfiI CCNNNNNNNGG 3 cut(s) 283, 684, 1168
AflII CTTAAG 1 cut(s) 1040
AgsI TTSAA 7 cut(s) 422, 566, 817, 884, 954, 1189, 1237
AhdI GACNNNNNGTC 2 cut(s) 534, 946
AjiI CACGTC 1 cut(s) 943
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AluBI AGCT 6 cut(s) 172, 489, 670, 694, 1021, 1039
AluI AGCT 6 cut(s) 172, 489, 670, 694, 1021, 1039
Alw21I GWGCWC 3 cut(s) 174, 491, 768
Alw26I GTCTC 1 cut(s) 123
Alw44I GTGCAC 1 cut(s) 764
AlwI GGATC 3 cut(s) 401, 442, 455
Ama87I CYCGRG 1 cut(s) 671
ApaLI GTGCAC 1 cut(s) 764
ApeKI GCWGC 1 cut(s) 162
ApoI RAATTY 2 cut(s) 423, 634
Asp700I GAANNNNTTC 2 cut(s) 36, 109
AspS9I GGNCC 3 cut(s) 675, 901, 1080
AsuC2I CCSGG 1 cut(s) 770
AsuHPI GGTGA 3 cut(s) 482, 720, 1153
AvaI CYCGRG 1 cut(s) 671
AvaII GGWCC 3 cut(s) 675, 901, 1080
AxyI CCTNAGG 1 cut(s) 1130
BaeGI GKGCMC 1 cut(s) 768
BamHI GGATCC 1 cut(s) 447
BanII GRGCYC 2 cut(s) 174, 491
BbsI GAAGAC 1 cut(s) 942
Bbv12I GWGCWC 3 cut(s) 174, 491, 768
BbvI GCAGC 1 cut(s) 149
BccI CCATC 4 cut(s) 285, 459, 641, 809
BcnI CCSGG 1 cut(s) 770
BcoDI GTCTC 1 cut(s) 123
BfaI CTAG 3 cut(s) 53, 243, 455
BfrI CTTAAG 1 cut(s) 1040
BfuAI ACCTGC 1 cut(s) 1221
BglII AGATCT 1 cut(s) 721
BisI GCNGC 2 cut(s) 163, 959
BlpI GCTNAGC 1 cut(s) 690
BlsI GCNGC 2 cut(s) 164, 960
Bme1390I CCNGG 1 cut(s) 770
Bme18I GGWCC 3 cut(s) 675, 901, 1080
BmeRI GACNNNNNGTC 2 cut(s) 534, 946
BmeT110I CYCGRG 1 cut(s) 671
BmgBI CACGTC 1 cut(s) 943
BmgT120I GGNCC 3 cut(s) 675, 901, 1080
BmiI GGNNCC 4 cut(s) 449, 676, 903, 1081
BmrFI CCNGG 1 cut(s) 770
BmrI ACTGGG 1 cut(s) 183
BmsI GCATC 2 cut(s) 1108, 1183
BmuI ACTGGG 1 cut(s) 183
BpiI GAAGAC 1 cut(s) 942
BpmI CTGGAG 1 cut(s) 1027
Bpu1102I GCTNAGC 1 cut(s) 690
BpuEI CTTGAG 2 cut(s) 1133, 1163
BpuMI CCSGG 1 cut(s) 770
BsaAI YACGTR 1 cut(s) 716
BsaHI GRCGYC 2 cut(s) 321, 376
BsaI GGTCTC 1 cut(s) 123
BsaJI CCNNGG 1 cut(s) 1162
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 684, 1168
Bse1I ACTGG 2 cut(s) 189, 1010
Bse21I CCTNAGG 1 cut(s) 1130
Bse3DI GCAATG 1 cut(s) 924
BseDI CCNNGG 1 cut(s) 1162
BseGI GGATG 2 cut(s) 652, 1123
BseLI CCNNNNNNNGG 3 cut(s) 283, 684, 1168
BseMI GCAATG 1 cut(s) 924
BseMII CTCAG 4 cut(s) 65, 681, 1049, 1121
BseNI ACTGG 2 cut(s) 189, 1010
BseSI GKGCMC 1 cut(s) 768
BseXI GCAGC 1 cut(s) 149
Bsh1236I CGCG 1 cut(s) 327
Bsh1285I CGRYCG 1 cut(s) 556
BsiEI CGRYCG 1 cut(s) 556
BsiHKAI GWGCWC 3 cut(s) 174, 491, 768
BsiHKCI CYCGRG 1 cut(s) 671
BsiSI CCGG 1 cut(s) 769
BslFI GGGAC 2 cut(s) 688, 887
BslI CCNNNNNNNGG 3 cut(s) 283, 684, 1168
BsmAI GTCTC 1 cut(s) 123
BsmFI GGGAC 2 cut(s) 688, 887
Bso31I GGTCTC 1 cut(s) 123
BsoBI CYCGRG 1 cut(s) 671
Bsp1286I GDGCHC 3 cut(s) 174, 491, 768
Bsp1407I TGTACA 1 cut(s) 599
Bsp143I GATC 3 cut(s) 393, 447, 721
Bsp1720I GCTNAGC 1 cut(s) 690
BspACI CCGC 3 cut(s) 325, 830, 959
BspCNI CTCAG 4 cut(s) 66, 682, 1048, 1122
BspFNI CGCG 1 cut(s) 327
BspLI GGNNCC 4 cut(s) 449, 676, 903, 1081
BspMI ACCTGC 1 cut(s) 1221
BspPI GGATC 3 cut(s) 401, 442, 455
BspTI CTTAAG 1 cut(s) 1040
BspTNI GGTCTC 1 cut(s) 123
BsrDI GCAATG 1 cut(s) 924
BsrGI TGTACA 1 cut(s) 599
BsrI ACTGG 2 cut(s) 189, 1010
BssECI CCNNGG 1 cut(s) 1162
BssMI GATC 3 cut(s) 393, 447, 721
BssNI GRCGYC 2 cut(s) 321, 376
BssT1I CCWWGG 1 cut(s) 1162
Bst4CI ACNGT 5 cut(s) 524, 532, 607, 658, 1054
BstACI GRCGYC 2 cut(s) 321, 376
BstAFI CTTAAG 1 cut(s) 1040
BstAUI TGTACA 1 cut(s) 599
BstBAI YACGTR 1 cut(s) 716
BstC8I GCNNGC 2 cut(s) 764, 1019
BstDEI CTNAG 8 cut(s) 74, 271, 679, 690, 835, 911, 1035, 1130
BstEII GGTNACC 1 cut(s) 1048
BstENI CCTNNNNNAGG 2 cut(s) 682, 1166
BstF5I GGATG 2 cut(s) 652, 1123
BstFNI CGCG 1 cut(s) 327
BstKTI GATC 3 cut(s) 396, 450, 724
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 3 cut(s) 393, 447, 721
BstMCI CGRYCG 1 cut(s) 556
BstPI GGTNACC 1 cut(s) 1048
BstSCI CCNGG 1 cut(s) 768
BstSLI GKGCMC 1 cut(s) 768
BstSNI TACGTA 1 cut(s) 716
BstUI CGCG 1 cut(s) 327
BstV1I GCAGC 1 cut(s) 149
BstV2I GAAGAC 1 cut(s) 942
BstX2I RGATCY 2 cut(s) 447, 721
BstYI RGATCY 2 cut(s) 447, 721
Bsu36I CCTNAGG 1 cut(s) 1130
BtrI CACGTC 1 cut(s) 943
BtsCI GGATG 2 cut(s) 652, 1123
BtsI GCAGTG 1 cut(s) 647
BtsIMutI CAGTG 1 cut(s) 647
BveI ACCTGC 1 cut(s) 1221
Cac8I GCNNGC 2 cut(s) 764, 1019
Cfr13I GGNCC 3 cut(s) 675, 901, 1080
Csp6I GTAC 3 cut(s) 21, 572, 600
CspCI CAANNNNNGTGG 2 cut(s) 240, 275
CviAII CATG 3 cut(s) 101, 354, 1217
CviQI GTAC 3 cut(s) 21, 572, 600
DdeI CTNAG 8 cut(s) 74, 271, 679, 690, 835, 911, 1035, 1130
DpnI GATC 3 cut(s) 395, 449, 723
DpnII GATC 3 cut(s) 393, 447, 721
DriI GACNNNNNGTC 2 cut(s) 534, 946
Eam1105I GACNNNNNGTC 2 cut(s) 534, 946
Ecl136II GAGCTC 2 cut(s) 172, 489
Eco105I TACGTA 1 cut(s) 716
Eco130I CCWWGG 1 cut(s) 1162
Eco24I GRGCYC 2 cut(s) 174, 491
Eco31I GGTCTC 1 cut(s) 123
Eco32I GATATC 2 cut(s) 439, 475
Eco47I GGWCC 3 cut(s) 675, 901, 1080
Eco53kI GAGCTC 2 cut(s) 172, 489
Eco57I CTGAAG 1 cut(s) 606
Eco81I CCTNAGG 1 cut(s) 1130
Eco88I CYCGRG 1 cut(s) 671
Eco91I GGTNACC 1 cut(s) 1048
EcoICRI GAGCTC 2 cut(s) 172, 489
EcoNI CCTNNNNNAGG 2 cut(s) 682, 1166
EcoO109I RGGNCCY 1 cut(s) 675
EcoO65I GGTNACC 1 cut(s) 1048
EcoRV GATATC 2 cut(s) 439, 475
EcoT14I CCWWGG 1 cut(s) 1162
EcoT22I ATGCAT 1 cut(s) 359
EcoT38I GRGCYC 2 cut(s) 174, 491
ErhI CCWWGG 1 cut(s) 1162
FaeI CATG 3 cut(s) 104, 357, 1220
FaqI GGGAC 2 cut(s) 688, 887
FatI CATG 3 cut(s) 100, 353, 1216
FblI GTMKAC 2 cut(s) 538, 1056
Fnu4HI GCNGC 2 cut(s) 163, 959
FokI GGATG 2 cut(s) 659, 1130
FriOI GRGCYC 2 cut(s) 174, 491
Fsp4HI GCNGC 2 cut(s) 163, 959
FspBI CTAG 3 cut(s) 53, 243, 455
GluI GCNGC 2 cut(s) 163, 959
GsuI CTGGAG 1 cut(s) 1027
HapII CCGG 1 cut(s) 769
Hin1I GRCGYC 2 cut(s) 321, 376
Hin1II CATG 3 cut(s) 104, 357, 1220
HincII GTYRAC 4 cut(s) 183, 539, 661, 748
HindII GTYRAC 4 cut(s) 183, 539, 661, 748
HindIII AAGCTT 1 cut(s) 1019
HinfI GANTC 2 cut(s) 105, 294
HpaII CCGG 1 cut(s) 769
HphI GGTGA 3 cut(s) 482, 720, 1153
Hpy166II GTNNAC 7 cut(s) 183, 539, 600, 661, 748, 766, 1057
Hpy188I TCNGA 4 cut(s) 88, 197, 592, 625
Hpy188III TCNNGA 6 cut(s) 53, 428, 455, 673, 817, 1180
Hpy8I GTNNAC 7 cut(s) 183, 539, 600, 661, 748, 766, 1057
Hpy99I CGWCG 2 cut(s) 381, 540
HpyAV CCTTC 3 cut(s) 308, 733, 980
HpyCH4III ACNGT 5 cut(s) 524, 532, 607, 658, 1054
HpyCH4IV ACGT 5 cut(s) 321, 376, 715, 843, 942
HpyCH4V TGCA 5 cut(s) 165, 357, 414, 640, 766
HpyF3I CTNAG 8 cut(s) 74, 271, 679, 690, 835, 911, 1035, 1130
HpySE526I ACGT 5 cut(s) 321, 376, 715, 843, 942
Hsp92I GRCGYC 2 cut(s) 321, 376
Hsp92II CATG 3 cut(s) 104, 357, 1220
Kzo9I GATC 3 cut(s) 393, 447, 721
LmnI GCTCC 2 cut(s) 169, 177
Lsp1109I GCAGC 1 cut(s) 149
LweI GCATC 2 cut(s) 1108, 1183
MaeI CTAG 3 cut(s) 53, 243, 455
MaeII ACGT 5 cut(s) 321, 376, 715, 843, 942
MaeIII GTNAC 1 cut(s) 1048
MalI GATC 3 cut(s) 395, 449, 723
MboI GATC 3 cut(s) 393, 447, 721
MboII GAAGA 3 cut(s) 578, 942, 1213
MflI RGATCY 2 cut(s) 447, 721
MhlI GDGCHC 3 cut(s) 174, 491, 768
MluCI AATT 6 cut(s) 93, 423, 634, 864, 884, 925
MmeI TCCRAC 2 cut(s) 506, 1107
MnlI CCTC 7 cut(s) 143, 358, 1002, 1039, 1040, 1108, 1125
Mph1103I ATGCAT 1 cut(s) 359
MroXI GAANNNNTTC 2 cut(s) 36, 109
MseI TTAA 7 cut(s) 407, 630, 774, 782, 929, 1041, 1062
MspCI CTTAAG 1 cut(s) 1040
MspI CCGG 1 cut(s) 769
MspR9I CCNGG 1 cut(s) 770
MvnI CGCG 1 cut(s) 327
NciI CCSGG 1 cut(s) 770
NdeII GATC 3 cut(s) 393, 447, 721
NlaIII CATG 3 cut(s) 104, 357, 1220
NlaIV GGNNCC 4 cut(s) 449, 676, 903, 1081
NsiI ATGCAT 1 cut(s) 359
PdmI GAANNNNTTC 2 cut(s) 36, 109
PfeI GAWTC 2 cut(s) 105, 294
PkrI GCNGC 2 cut(s) 164, 960
Ppu21I YACGTR 1 cut(s) 716
PpuMI RGGWCCY 1 cut(s) 675
Psp124BI GAGCTC 2 cut(s) 174, 491
Psp1406I AACGTT 1 cut(s) 843
Psp5II RGGWCCY 1 cut(s) 675
PspEI GGTNACC 1 cut(s) 1048
PspN4I GGNNCC 4 cut(s) 449, 676, 903, 1081
PspPI GGNCC 3 cut(s) 675, 901, 1080
PspPPI RGGWCCY 1 cut(s) 675
PsuI RGATCY 2 cut(s) 447, 721
RsaI GTAC 3 cut(s) 22, 573, 601
RsaNI GTAC 3 cut(s) 21, 572, 600
SacI GAGCTC 2 cut(s) 174, 491
SalI GTCGAC 1 cut(s) 537
SaqAI TTAA 7 cut(s) 407, 630, 774, 782, 929, 1041, 1062
SatI GCNGC 2 cut(s) 163, 959
Sau3AI GATC 3 cut(s) 393, 447, 721
Sau96I GGNCC 3 cut(s) 675, 901, 1080
ScrFI CCNGG 1 cut(s) 770
SduI GDGCHC 3 cut(s) 174, 491, 768
SfaNI GCATC 2 cut(s) 1108, 1183
SinI GGWCC 3 cut(s) 675, 901, 1080
SmlI CTYRAG 3 cut(s) 1040, 1112, 1178
SmoI CTYRAG 3 cut(s) 1040, 1112, 1178
SnaBI TACGTA 1 cut(s) 716
Sse9I AATT 6 cut(s) 93, 423, 634, 864, 884, 925
SsiI CCGC 3 cut(s) 325, 830, 959
SspMI CTAG 3 cut(s) 53, 243, 455
SstI GAGCTC 2 cut(s) 174, 491
StyD4I CCNGG 1 cut(s) 768
StyI CCWWGG 1 cut(s) 1162
TaaI ACNGT 5 cut(s) 524, 532, 607, 658, 1054
TaiI ACGT 5 cut(s) 324, 379, 718, 846, 945
TaqI TCGA 5 cut(s) 396, 515, 538, 1066, 1092
TaqII GACCGA 1 cut(s) 542
TasI AATT 6 cut(s) 93, 423, 634, 864, 884, 925
TatI WGTACW 1 cut(s) 599
TauI GCSGC 1 cut(s) 961
TfiI GAWTC 2 cut(s) 105, 294
Tru1I TTAA 7 cut(s) 407, 630, 774, 782, 929, 1041, 1062
Tru9I TTAA 7 cut(s) 407, 630, 774, 782, 929, 1041, 1062
TscAI CASTG 1 cut(s) 647
TseI GCWGC 1 cut(s) 162
TspDTI ATGAA 6 cut(s) 290, 360, 875, 993, 1087, 1206
TspRI CASTG 1 cut(s) 647
Vha464I CTTAAG 1 cut(s) 1040
VneI GTGCAC 1 cut(s) 764
VpaK11BI GGWCC 3 cut(s) 675, 901, 1080
XagI CCTNNNNNAGG 2 cut(s) 682, 1166
XapI RAATTY 2 cut(s) 423, 634
XbaI TCTAGA 2 cut(s) 52, 454
XcmI CCANNNNNNNNNTGG 1 cut(s) 458
XmiI GTMKAC 2 cut(s) 538, 1056
XmnI GAANNNNTTC 2 cut(s) 36, 109
XspI CTAG 3 cut(s) 53, 243, 455
ZraI GACGTC 2 cut(s) 322, 377
Zsp2I ATGCAT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.