MD12G1217800.v1.1

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0024428
Physical Location & Seq
Reverse (-)
29538501 .. 29541246
2746 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1217800.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGGAGACTTGTAGGGCAAGTAAGAGTAGTGAAAAAGTTCCGAGAGTTAGCTTAGAGTTTAGGGTGGATAGGCTGAGTAGTCTTCCAGACGAAATTACTCATAGAATCCTCTCGTTGCTTCCATTTGGAGACCTCTCTCGCTTTGGGAGTTTATCCAAACGCTGCAGGGAGCTCCATTTGTCAACTCCCAGTTTCAGATTAGATTTGTCTAAAGTTGATATATCAACTTGTGATAAGTGGTCTAGGTTCGCAAATTCTGTGGATAGGTTCTTAGACCATCGTGGGGAGAGCAGAATCCTTCACTTTGGTATTAGATTCGACGTCCGTGAACATATTTTTGGAAATGAAACCCCATGCATTTGTGAGGGGGTAGGACGTCGGTTTATTTCGTGGATCGAAAATGCTTTAAGGTGCAAGGTTGAAATACTTGACCTTGATATCAGTAGGATCCATCTAGCGAATGGGGTAGGTGATATCACCATTGAAAGCTCATCACTAAAATCATTTAGTATTTCGAGCAACAGTTGGACAGTTCGTCGACTTAAAATCATCGGTCATAAGCTTGAAGATGTACGCATAGATTGGCGTTCGGAGTGGTGTACAACAGTAAGCATATTTGCTTCAGATGTCAAGAATTTGGAGTGGGATGGGATTACTGTCAACCACCAGCTCGGGACCTTAGAAAGGGCTGAGCTTTCTTTGGCCGATATAATACGTAGAGATCTATCACCTGATGTGAAGGCAGTTGACTTCCTTACCAGCGTGCACCGGGTTAAAGCTTTAACTCTAAATCACAATACCATCAAGGCTTTGTTCAAGAAAGGAATACCGCCCTTAGAGAACGTTTTTTATTTCTCTATGAAAATTGGAAACTTTAGTATTGAATTGGTGTTGGCATTGGTCCCTATTCTTAGGGCAATGCCTAATTTAACTATCTTGGACGTGAAGTCTTCGCCGCCCCGTTGCATTCTAAATGAATGTTCAGGTCTTACTATGGAATACTGGAGGTCAAGCAACCTTCCTTTCCTCTCTCAGCTTAAGGAGGTAACCGTAGACCTTAATCGACACTTCTACGGGACCAACCAAATCGAGTTCATAAGGTTTATCCTTGAGGATGCTCAAAGCCTGAGGAAAATGGTGATTATTCATCCATTAGGCCTTGGAAAGGGGCATCTGAAGAGGTTGAATGAAAGTGAGAAGACTTCCAATGCTCGCATAGTCTATCGCAGGTTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

413

Amino Acids

47.26

Weight (kDa)

9.26

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 25 - 58 3.5e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024428)

Species Orthologous Gene IDs
malus_domestica MD12G1217700.v1.1 MD12G1217800.v1.1
pyrus_communis pycom12g20330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 324, 379
Acc36I ACCTGC 1 cut(s) 1218
AccI GTMKAC 2 cut(s) 538, 1053
AciI CCGC 2 cut(s) 830, 956
AclI AACGTT 1 cut(s) 843
AclWI GGATC 3 cut(s) 401, 442, 455
AcoI YGGCCR 1 cut(s) 702
AcsI RAATTY 2 cut(s) 253, 634
AcuI CTGAAG 2 cut(s) 606, 1196
AcyI GRCGYC 2 cut(s) 321, 376
AfaI GTAC 2 cut(s) 573, 601
AfiI CCNNNNNNNGG 3 cut(s) 283, 684, 1165
AflII CTTAAG 1 cut(s) 1037
AgsI TTSAA 7 cut(s) 422, 485, 566, 817, 884, 1186, 1234
AhdI GACNNNNNGTC 2 cut(s) 534, 946
AjiI CACGTC 1 cut(s) 943
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AluBI AGCT 8 cut(s) 51, 172, 489, 562, 670, 694, 779, 1036
AluI AGCT 8 cut(s) 51, 172, 489, 562, 670, 694, 779, 1036
Alw21I GWGCWC 2 cut(s) 174, 768
Alw26I GTCTC 1 cut(s) 123
Alw44I GTGCAC 1 cut(s) 764
AlwI GGATC 3 cut(s) 401, 442, 455
Ama87I CYCGRG 1 cut(s) 671
AoxI GGCC 2 cut(s) 702, 1156
ApaLI GTGCAC 1 cut(s) 764
ApeKI GCWGC 1 cut(s) 162
ApoI RAATTY 2 cut(s) 253, 634
Asp700I GAANNNNTTC 1 cut(s) 36
AspS9I GGNCC 3 cut(s) 675, 901, 1077
AsuC2I CCSGG 1 cut(s) 770
AsuHPI GGTGA 4 cut(s) 469, 482, 720, 1150
AvaI CYCGRG 1 cut(s) 671
AvaII GGWCC 3 cut(s) 675, 901, 1077
AxyI CCTNAGG 1 cut(s) 1127
BaeGI GKGCMC 1 cut(s) 768
BamHI GGATCC 1 cut(s) 447
BanII GRGCYC 1 cut(s) 174
BbsI GAAGAC 3 cut(s) 74, 942, 1205
Bbv12I GWGCWC 2 cut(s) 174, 768
BbvI GCAGC 1 cut(s) 149
BccI CCATC 4 cut(s) 285, 459, 641, 809
BcnI CCSGG 1 cut(s) 770
BcoDI GTCTC 1 cut(s) 123
BfaI CTAG 2 cut(s) 243, 455
BfmI CTRYAG 1 cut(s) 163
BfrI CTTAAG 1 cut(s) 1037
BfuAI ACCTGC 1 cut(s) 1218
BglII AGATCT 1 cut(s) 721
BisI GCNGC 2 cut(s) 163, 956
BlpI GCTNAGC 1 cut(s) 690
BlsI GCNGC 2 cut(s) 164, 957
Bme1390I CCNGG 1 cut(s) 770
Bme18I GGWCC 3 cut(s) 675, 901, 1077
BmeRI GACNNNNNGTC 2 cut(s) 534, 946
BmeT110I CYCGRG 1 cut(s) 671
BmgBI CACGTC 1 cut(s) 943
BmgT120I GGNCC 3 cut(s) 675, 901, 1077
BmiI GGNNCC 4 cut(s) 449, 676, 903, 1078
BmrFI CCNGG 1 cut(s) 770
BmrI ACTGGG 1 cut(s) 183
BmsI GCATC 2 cut(s) 1105, 1180
BmuI ACTGGG 1 cut(s) 183
BpiI GAAGAC 3 cut(s) 74, 942, 1205
BpmI CTGGAG 1 cut(s) 1024
Bpu1102I GCTNAGC 1 cut(s) 690
BpuEI CTTGAG 1 cut(s) 1130
BpuMI CCSGG 1 cut(s) 770
BsaAI YACGTR 1 cut(s) 716
BsaHI GRCGYC 2 cut(s) 321, 376
BsaI GGTCTC 1 cut(s) 123
BsaJI CCNNGG 1 cut(s) 1159
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 684, 1165
Bse1I ACTGG 2 cut(s) 189, 1007
Bse21I CCTNAGG 1 cut(s) 1127
Bse3DI GCAATG 1 cut(s) 924
BseDI CCNNGG 1 cut(s) 1159
BseGI GGATG 3 cut(s) 652, 1120, 1147
BseLI CCNNNNNNNGG 3 cut(s) 283, 684, 1165
BseMI GCAATG 1 cut(s) 924
BseMII CTCAG 4 cut(s) 65, 681, 1046, 1118
BseNI ACTGG 2 cut(s) 189, 1007
BseSI GKGCMC 1 cut(s) 768
BseXI GCAGC 1 cut(s) 149
BshFI GGCC 2 cut(s) 704, 1158
BsiHKAI GWGCWC 2 cut(s) 174, 768
BsiHKCI CYCGRG 1 cut(s) 671
BsiSI CCGG 1 cut(s) 769
BslFI GGGAC 3 cut(s) 688, 887, 1090
BslI CCNNNNNNNGG 3 cut(s) 283, 684, 1165
BsmAI GTCTC 1 cut(s) 123
BsmFI GGGAC 3 cut(s) 688, 887, 1090
BsmI GAATGC 1 cut(s) 966
BsnI GGCC 2 cut(s) 704, 1158
Bso31I GGTCTC 1 cut(s) 123
BsoBI CYCGRG 1 cut(s) 671
Bsp1286I GDGCHC 2 cut(s) 174, 768
Bsp1407I TGTACA 1 cut(s) 599
Bsp143I GATC 3 cut(s) 393, 447, 721
Bsp1720I GCTNAGC 1 cut(s) 690
BspACI CCGC 2 cut(s) 830, 956
BspANI GGCC 2 cut(s) 704, 1158
BspCNI CTCAG 4 cut(s) 66, 682, 1045, 1119
BspLI GGNNCC 4 cut(s) 449, 676, 903, 1078
BspMAI CTGCAG 1 cut(s) 167
BspMI ACCTGC 1 cut(s) 1218
BspPI GGATC 3 cut(s) 401, 442, 455
BspTI CTTAAG 1 cut(s) 1037
BspTNI GGTCTC 1 cut(s) 123
BsrDI GCAATG 1 cut(s) 924
BsrGI TGTACA 1 cut(s) 599
BsrI ACTGG 2 cut(s) 189, 1007
BssECI CCNNGG 1 cut(s) 1159
BssMI GATC 3 cut(s) 393, 447, 721
BssNI GRCGYC 2 cut(s) 321, 376
BssT1I CCWWGG 1 cut(s) 1159
Bst4CI ACNGT 5 cut(s) 524, 532, 607, 658, 1051
Bst6I CTCTTC 1 cut(s) 1172
BstACI GRCGYC 2 cut(s) 321, 376
BstAFI CTTAAG 1 cut(s) 1037
BstAUI TGTACA 1 cut(s) 599
BstBAI YACGTR 1 cut(s) 716
BstC8I GCNNGC 2 cut(s) 764, 1213
BstDEI CTNAG 9 cut(s) 52, 74, 271, 679, 690, 835, 911, 1032, 1127
BstEII GGTNACC 1 cut(s) 1045
BstENI CCTNNNNNAGG 2 cut(s) 682, 1163
BstF5I GGATG 3 cut(s) 652, 1120, 1147
BstKTI GATC 3 cut(s) 396, 450, 724
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 3 cut(s) 393, 447, 721
BstPI GGTNACC 1 cut(s) 1045
BstSCI CCNGG 1 cut(s) 768
BstSFI CTRYAG 1 cut(s) 163
BstSLI GKGCMC 1 cut(s) 768
BstSNI TACGTA 1 cut(s) 716
BstV1I GCAGC 1 cut(s) 149
BstV2I GAAGAC 3 cut(s) 74, 942, 1205
BstX2I RGATCY 2 cut(s) 447, 721
BstYI RGATCY 2 cut(s) 447, 721
Bsu36I CCTNAGG 1 cut(s) 1127
BsuRI GGCC 2 cut(s) 704, 1158
BtrI CACGTC 1 cut(s) 943
BtsCI GGATG 3 cut(s) 652, 1120, 1147
BveI ACCTGC 1 cut(s) 1218
Cac8I GCNNGC 2 cut(s) 764, 1213
Cfr13I GGNCC 3 cut(s) 675, 901, 1077
Csp6I GTAC 2 cut(s) 572, 600
CspCI CAANNNNNGTGG 2 cut(s) 240, 275
CviAII CATG 1 cut(s) 354
CviQI GTAC 2 cut(s) 572, 600
DdeI CTNAG 9 cut(s) 52, 74, 271, 679, 690, 835, 911, 1032, 1127
DpnI GATC 3 cut(s) 395, 449, 723
DpnII GATC 3 cut(s) 393, 447, 721
DriI GACNNNNNGTC 2 cut(s) 534, 946
EaeI YGGCCR 1 cut(s) 702
Eam1104I CTCTTC 1 cut(s) 1172
Eam1105I GACNNNNNGTC 2 cut(s) 534, 946
EarI CTCTTC 1 cut(s) 1172
Ecl136II GAGCTC 1 cut(s) 172
Eco105I TACGTA 1 cut(s) 716
Eco130I CCWWGG 1 cut(s) 1159
Eco147I AGGCCT 1 cut(s) 1158
Eco24I GRGCYC 1 cut(s) 174
Eco31I GGTCTC 1 cut(s) 123
Eco32I GATATC 2 cut(s) 439, 475
Eco47I GGWCC 3 cut(s) 675, 901, 1077
Eco53kI GAGCTC 1 cut(s) 172
Eco57I CTGAAG 2 cut(s) 606, 1196
Eco81I CCTNAGG 1 cut(s) 1127
Eco88I CYCGRG 1 cut(s) 671
Eco91I GGTNACC 1 cut(s) 1045
EcoICRI GAGCTC 1 cut(s) 172
EcoNI CCTNNNNNAGG 2 cut(s) 682, 1163
EcoO109I RGGNCCY 1 cut(s) 675
EcoO65I GGTNACC 1 cut(s) 1045
EcoRV GATATC 2 cut(s) 439, 475
EcoT14I CCWWGG 1 cut(s) 1159
EcoT22I ATGCAT 1 cut(s) 359
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 1159
FaeI CATG 1 cut(s) 357
FaqI GGGAC 3 cut(s) 688, 887, 1090
FatI CATG 1 cut(s) 353
FblI GTMKAC 2 cut(s) 538, 1053
Fnu4HI GCNGC 2 cut(s) 163, 956
FokI GGATG 3 cut(s) 659, 1127, 1134
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 2 cut(s) 163, 956
FspBI CTAG 2 cut(s) 243, 455
GluI GCNGC 2 cut(s) 163, 956
GsuI CTGGAG 1 cut(s) 1024
HaeIII GGCC 2 cut(s) 704, 1158
HapII CCGG 1 cut(s) 769
Hin1I GRCGYC 2 cut(s) 321, 376
Hin1II CATG 1 cut(s) 357
HincII GTYRAC 4 cut(s) 183, 539, 661, 748
HindII GTYRAC 4 cut(s) 183, 539, 661, 748
HindIII AAGCTT 2 cut(s) 560, 777
HinfI GANTC 3 cut(s) 105, 294, 315
HpaII CCGG 1 cut(s) 769
HphI GGTGA 4 cut(s) 469, 482, 720, 1150
Hpy166II GTNNAC 8 cut(s) 183, 329, 539, 600, 661, 748, 766, 1054
Hpy188I TCNGA 5 cut(s) 42, 197, 592, 625, 1176
Hpy188III TCNNGA 4 cut(s) 86, 631, 673, 817
Hpy8I GTNNAC 8 cut(s) 183, 329, 539, 600, 661, 748, 766, 1054
Hpy99I CGWCG 3 cut(s) 323, 381, 540
HpyAV CCTTC 3 cut(s) 308, 733, 1028
HpyCH4III ACNGT 5 cut(s) 524, 532, 607, 658, 1051
HpyCH4IV ACGT 5 cut(s) 321, 376, 715, 843, 942
HpyCH4V TGCA 5 cut(s) 165, 357, 414, 766, 966
HpyF3I CTNAG 9 cut(s) 52, 74, 271, 679, 690, 835, 911, 1032, 1127
HpySE526I ACGT 5 cut(s) 321, 376, 715, 843, 942
Hsp92I GRCGYC 2 cut(s) 321, 376
Hsp92II CATG 1 cut(s) 357
Kzo9I GATC 3 cut(s) 393, 447, 721
LmnI GCTCC 2 cut(s) 169, 177
Lsp1109I GCAGC 1 cut(s) 149
LweI GCATC 2 cut(s) 1105, 1180
MaeI CTAG 2 cut(s) 243, 455
MaeII ACGT 5 cut(s) 321, 376, 715, 843, 942
MaeIII GTNAC 1 cut(s) 1045
MalI GATC 3 cut(s) 395, 449, 723
MboI GATC 3 cut(s) 393, 447, 721
MboII GAAGA 5 cut(s) 74, 578, 942, 1189, 1210
MflI RGATCY 2 cut(s) 447, 721
MhlI GDGCHC 2 cut(s) 174, 768
MluCI AATT 6 cut(s) 93, 253, 634, 864, 884, 925
MmeI TCCRAC 1 cut(s) 506
MnlI CCTC 9 cut(s) 119, 143, 358, 999, 1036, 1037, 1105, 1122, 1173
Mph1103I ATGCAT 1 cut(s) 359
MroXI GAANNNNTTC 1 cut(s) 36
MseI TTAA 7 cut(s) 407, 543, 774, 782, 929, 1038, 1059
MspCI CTTAAG 1 cut(s) 1037
MspI CCGG 1 cut(s) 769
MspR9I CCNGG 1 cut(s) 770
Mva1269I GAATGC 1 cut(s) 966
NciI CCSGG 1 cut(s) 770
NdeII GATC 3 cut(s) 393, 447, 721
NlaIII CATG 1 cut(s) 357
NlaIV GGNNCC 4 cut(s) 449, 676, 903, 1078
NsiI ATGCAT 1 cut(s) 359
PceI AGGCCT 1 cut(s) 1158
PctI GAATGC 1 cut(s) 966
PdmI GAANNNNTTC 1 cut(s) 36
PfeI GAWTC 3 cut(s) 105, 294, 315
PkrI GCNGC 2 cut(s) 164, 957
Ppu21I YACGTR 1 cut(s) 716
PpuMI RGGWCCY 1 cut(s) 675
Psp124BI GAGCTC 1 cut(s) 174
Psp1406I AACGTT 1 cut(s) 843
Psp5II RGGWCCY 1 cut(s) 675
PspEI GGTNACC 1 cut(s) 1045
PspN4I GGNNCC 4 cut(s) 449, 676, 903, 1078
PspPI GGNCC 3 cut(s) 675, 901, 1077
PspPPI RGGWCCY 1 cut(s) 675
PstI CTGCAG 1 cut(s) 167
PsuI RGATCY 2 cut(s) 447, 721
RsaI GTAC 2 cut(s) 573, 601
RsaNI GTAC 2 cut(s) 572, 600
SacI GAGCTC 1 cut(s) 174
SalI GTCGAC 1 cut(s) 537
SaqAI TTAA 7 cut(s) 407, 543, 774, 782, 929, 1038, 1059
SatI GCNGC 2 cut(s) 163, 956
Sau3AI GATC 3 cut(s) 393, 447, 721
Sau96I GGNCC 3 cut(s) 675, 901, 1077
ScrFI CCNGG 1 cut(s) 770
SduI GDGCHC 2 cut(s) 174, 768
SfaNI GCATC 2 cut(s) 1105, 1180
SfcI CTRYAG 1 cut(s) 163
SinI GGWCC 3 cut(s) 675, 901, 1077
SmlI CTYRAG 2 cut(s) 1037, 1109
SmoI CTYRAG 2 cut(s) 1037, 1109
SnaBI TACGTA 1 cut(s) 716
Sse9I AATT 6 cut(s) 93, 253, 634, 864, 884, 925
SseBI AGGCCT 1 cut(s) 1158
SsiI CCGC 2 cut(s) 830, 956
SspMI CTAG 2 cut(s) 243, 455
SstI GAGCTC 1 cut(s) 174
StuI AGGCCT 1 cut(s) 1158
StyD4I CCNGG 1 cut(s) 768
StyI CCWWGG 1 cut(s) 1159
TaaI ACNGT 5 cut(s) 524, 532, 607, 658, 1051
TaiI ACGT 5 cut(s) 324, 379, 718, 846, 945
TaqI TCGA 6 cut(s) 318, 396, 515, 538, 1063, 1089
TaqII GACCGA 1 cut(s) 542
TasI AATT 6 cut(s) 93, 253, 634, 864, 884, 925
TatI WGTACW 1 cut(s) 599
TauI GCSGC 1 cut(s) 958
TfiI GAWTC 3 cut(s) 105, 294, 315
Tru1I TTAA 7 cut(s) 407, 543, 774, 782, 929, 1038, 1059
Tru9I TTAA 7 cut(s) 407, 543, 774, 782, 929, 1038, 1059
TseI GCWGC 1 cut(s) 162
TspDTI ATGAA 6 cut(s) 360, 875, 990, 1084, 1136, 1203
TspGWI ACGGA 1 cut(s) 314
Vha464I CTTAAG 1 cut(s) 1037
VneI GTGCAC 1 cut(s) 764
VpaK11BI GGWCC 3 cut(s) 675, 901, 1077
XagI CCTNNNNNAGG 2 cut(s) 682, 1163
XapI RAATTY 2 cut(s) 253, 634
XcmI CCANNNNNNNNNTGG 1 cut(s) 458
XmiI GTMKAC 2 cut(s) 538, 1053
XmnI GAANNNNTTC 1 cut(s) 36
XspI CTAG 2 cut(s) 243, 455
ZraI GACGTC 2 cut(s) 322, 377
Zsp2I ATGCAT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.