Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0008598

Physical Location & Seq

Genomic Coordinates Forward (+)

29657240 .. 29663481

Gene Structure 6242 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1219400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2586 bp

ATGGAAGATCAAACGGTGGAAATGTTCCACGAAAACTGCACCGACGTCTTTCCAGATCACTACTCCAAGCCTTCCGCAACCTCCGAAGCAGTTAAAACCCTAGAGACGATAGCAAGCCGGGTGGACAGTGATGTGATCGACAAAGAATGTACTCCAGATATTGCTACCAAACCCGTCGACGGAGATTCTGATTTGATTGGTGAGAGTAGCGCGTCGAACGGAACAGAAGGTTCTCCATGTCAAAGGTTCCTTCCAGTTTTGCAGAAGATCACTGATTTGACAACCAAAATTCAGGATTTGAAGAAAGAGCATACAGTCTTGTCTGATCAAGTCAAGCTCACAACGAATTCCTTTCCGGACCCTTCTGTTCTAAACACCATTCAGCTACTAAGTATGGAACATGAACTTTTAAAGAAGAAGTACCTAGATGAGTCGTCCGAGCGAAAGAGACTTTACAATGAAGTGATTGAGCTTAAAGGCAACATCCGAGTTTTCTGCAGATGTAGACCTTTGAATCAAACTGAAATTTCAAGCGGATCTGGTTCCGTTGTTGAATTCGAATCCTCCCTAGATAACGAGCTGCAGGTCCTTTGTTCTGATTCCTCTAAAAAGCAGTTCAAGTTTGACCATGTCTTCAGACCTGAGGACAACCAAGAGGCTGTCTTTGCTCAAACGAAGCCTATTGTGACTTCGGTGTTGGATGGGTATAATGTCTGCATTTTTGCCTATGGACAAACTGGAACTGGCAAGACCTTTACGATGGAGGGTACTTCTGAAAACAGAGGTGTTAACTACAGGACTCTGGAGGAGTTGTTTCGGATTTCAAAAGACAGAGGTGGCTTTATGAGATATGAGTTATGTGTTAGTATGCTGGAGGTTTATAATGAGAAGATTAGGGACCTCTTGGTCGATAACACTAATCAACCCACAAGGAAGTTGGAAATAAAGCAATGTGCAGACGGAACCTTAGATGTTCCAGGACTTGTTGAAGAGCGTGTTTATGGCTTTGAAGAGATGTGGGAACTGCTAAAGTCTGGAAGCCAAGCAAGGTCTGTTGGATCTACCAGTGCTAACGAGCAGAGCAGCCGCTCTCACTGCTTGTTACGTGTGACAGTGAAGGGGGAGACCTTGATAAATGGGCAAAAGACAAGGAGTCAACTGTGGCTCGTAGACTTGGCTGGCAGTGAACGTGTGGGGAGGATTGATGTTGAAGGTGAAAGACTGAAGGAATCTCAGTTTATAAATAAATCACTCTCGGCCCTTGGCGATGTTATCTCATCCCTTGCATCCAAGACAGCCCACATTCCTTACAGGCAACTCTCTCTCTCTCTCTCTCTCTCACAGACACACACACACAAACACATTTGGCTACCATATCTAAACTTCTCTTTTTTGAAAAAAACCAGGAATTCGAAGCTTACTCATATGCTGCAGAGTTCTCTAGGGGGAGATTGCAAAACCTTGATGTTTGTCCAAATCAGCCCAAGTGCCTCAGATCTTGGAGAGACACTGTGTTCATTGAACTTTGCCAGCCGGGTGCGGGGAATAGAGAGTGGACCTGCTCGCAAACAGGCAGACCTCACTGAGCTGTTCAAGTACAAACAAATGGCTGAAAAGGCTAAACATGACGAGAAAGAAACAAAGAAACTACAGGATTGCATACAGTCTCTGCAGTTGAGGCTTGCTTCAAGGGAACACATTTGCAGAAATCTACAAGAAAAGAATCGGGACCTTGAGAACCAGTTGGCTGAGGAAAGGAAGACCAGACTGAAACAGGAGACTAGAGCTTTCGCAGCGGTTTCTCATCAGTCTTCAGCATCATCATTCCGGAAACAAGGAGCTCAGAAGACTGCTGCAGAGAAGAAACCGCCATTGGCTCCTCCAAGATCAAGGCTACCTTTGCGAAGAATTTCCAACTTCATGCCCCCATCATCTGCTTTACCGCCCAAAAAAACCATCTCTAGAACTCCCATCCCATCTTCAGTTGACGGAAAAGAAAACATCTCCACAACAGCACTAGCAGGAAGAAACCAGAAAAACCAAATTTTACCGAGAAGAATCTCCATTGCTGTCAGACCTCCACCCGCAACAACACAACAGGTTCTCCAGCCTAAGAGACGAGTCTCTATTGCTACATATCGCCCTGACCCGAACTCTCACATGACAACACCCCTCCACACCTCTGCCTCTCTATTTAACAGTGGCAGACTGTCGTTCGCAAGGGATCCACGAAAGTCACGGTATTCGAGGCTGTTCTCTCCGATGCCTGAATTGAAGTCGGAAGCAGAGACAACGCCAACCACAATGAGGAGAAGCAGCAAGTTCATGGGTAGTCCCCCGACACTGGCTGGTTCAGTCAGGGCAGCTAGGCATCCGGCAGCAGTTGCATTACAACGGAAACCAGTAATTTGGAGTCCACTCAAGCTGAGAGGCATGAAAAACAGAAGGCCATCACTGTTACTGCCTCTCCGAGTGGGAACGAATGGACATGGATCCTCTCCGGATCCAGTTGTTCTAATCCATATAGTCAAATCATTTGAGTTATTGAAATTTTATTCACCGGCTACGATCAAGGGTCCACTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin Microtub_bd

Protein Analysis

862

Amino Acids

96.33

Weight (kDa)

9.46

Isoelectric Point (pI)

47.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	143 - 302	5.5e-43	Microtubule binding
Kinesin	PF00225	167 - 442	3.9e-88	Kinesin motor domain
Kinesin	PF00225	469 - 516	5.3e-15	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008598)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27550 AT5G27550
fragaria_vesca	FvH4_6g05830 FvH4_6g05830 FvH4_6g05830 FvH4_6g05830 FvH4_6g05830
malus_domestica	MD04G1205300.v1.1 MD12G1219400.v1.1
prunus_persica	Prupe.6G326000_v2.0.a1 Prupe.6G326000_v2.0.a1
pyrus_communis	pycom04g18160 pycom12g20490
rosa_chinensis	RchiOBHm_Chr3g0454741
rosa_laevigata	RLG00000025419
rosa_multiflora	Rmu_sc0003140.1_g000019
rosa_roxburghii	Rroxscaffold_6G00424800
rosa_rugosa	Rorug03G0002100
rosa_samantha	Rh3AG062100 Rh3BG064000 Rh3CG062900 Rh3DG063700
rosa_wichuraiana	Rw3G004830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	882, 1241
AasI	GACNNNNNNGTC	1 cut(s)	905
AatII	GACGTC	1 cut(s)	48
Acc36I	ACCTGC	2 cut(s)	574, 1567
AccBSI	CCGCTC	1 cut(s)	1089
AccI	GTMKAC	3 cut(s)	177, 505, 1170
AccII	CGCG	1 cut(s)	212
AccIII	TCCGGA	3 cut(s)	355, 1827, 2500
AciI	CCGC	8 cut(s)	75, 534, 1087, 1540, 1796, 1868, 1943, 2085
AclWI	GGATC	8 cut(s)	544, 1066, 2219, 2232, 2487, 2498, 2500, 2511
AcsI	RAATTY	8 cut(s)	288, 346, 525, 554, 1408, 1908, 2043, 2549
AcuI	CTGAAG	4 cut(s)	619, 1244, 1797, 1965
AcyI	GRCGYC	1 cut(s)	45
AfaI	GTAC	4 cut(s)	151, 422, 769, 1598
AfiI	CCNNNNNNNGG	4 cut(s)	179, 1540, 2307, 2344
AflIII	ACRYGT	2 cut(s)	1105, 1189
AhdI	GACNNNNNGTC	1 cut(s)	1152
AjnI	CCWGG	2 cut(s)	976, 1403
AjuI	GAANNNNNNNTTGG	2 cut(s)	1856, 1888
Alw21I	GWGCWC	1 cut(s)	1843
Alw26I	GTCTC	9 cut(s)	98, 442, 1118, 1499, 1671, 1772, 2110, 2128, 2282
AlwI	GGATC	8 cut(s)	544, 1066, 2219, 2232, 2487, 2498, 2500, 2511
AlwNI	CAGNNNCTG	1 cut(s)	1669
Aor13HI	TCCGGA	3 cut(s)	355, 1827, 2500
AoxI	GGCC	2 cut(s)	1257, 2447
ApeKI	GCWGC	8 cut(s)	580, 1083, 1429, 1793, 1853, 2316, 2363, 2378
ApoI	RAATTY	8 cut(s)	288, 346, 525, 554, 1408, 1908, 2043, 2549
Asp700I	GAANNNNTTC	1 cut(s)	1909
AspLEI	GCGC	1 cut(s)	212
AspS9I	GGNCC	7 cut(s)	358, 586, 898, 1258, 1556, 1729, 2576
AsuC2I	CCSGG	2 cut(s)	119, 1535
AsuHPI	GGTGA	3 cut(s)	212, 1226, 2550
AsuII	TTCGAA	2 cut(s)	558, 1412
AvaII	GGWCC	6 cut(s)	358, 586, 898, 1556, 1729, 2576
AxyI	CCTNAGG	1 cut(s)	642
BamHI	GGATCC	3 cut(s)	2224, 2492, 2503
BanII	GRGCYC	1 cut(s)	1843
BbsI	GAAGAC	4 cut(s)	625, 1766, 1803, 1853
Bbv12I	GWGCWC	1 cut(s)	1843
BbvCI	CCTCAGC	1 cut(s)	1749
BbvI	GCAGC	8 cut(s)	567, 1095, 1416, 1805, 1840, 2328, 2375, 2390
BccI	CCATC	7 cut(s)	695, 754, 1936, 1964, 1979, 1984, 2458
BcgI	CGANNNNNNTGC	2 cut(s)	477, 511
BciT130I	CCWGG	2 cut(s)	978, 1405
BclI	TGATCA	1 cut(s)	325
BcnI	CCSGG	2 cut(s)	119, 1535
BcoDI	GTCTC	9 cut(s)	98, 442, 1118, 1499, 1671, 1772, 2110, 2128, 2282
BfaI	CTAG	8 cut(s)	101, 425, 569, 1442, 1782, 1962, 2018, 2367
BfmI	CTRYAG	7 cut(s)	496, 581, 793, 1430, 1649, 1670, 1854
BfuAI	ACCTGC	2 cut(s)	574, 1567
BglII	AGATCT	1 cut(s)	1495
BisI	GCNGC	9 cut(s)	581, 1084, 1087, 1430, 1794, 1854, 2317, 2364, 2379
BlsI	GCNGC	9 cut(s)	582, 1085, 1088, 1431, 1795, 1855, 2318, 2365, 2380
Bme1390I	CCNGG	4 cut(s)	119, 978, 1405, 1535
Bme18I	GGWCC	6 cut(s)	358, 586, 898, 1556, 1729, 2576
BmeRI	GACNNNNNGTC	1 cut(s)	1152
BmgT120I	GGNCC	7 cut(s)	358, 586, 898, 1258, 1556, 1729, 2576
BmrFI	CCNGG	4 cut(s)	119, 978, 1405, 1535
BmsI	GCATC	4 cut(s)	1295, 1826, 2253, 2380
BpiI	GAAGAC	4 cut(s)	625, 1766, 1803, 1853
BpmI	CTGGAG	4 cut(s)	138, 824, 893, 2090
Bpu10I	CCTNAGC	1 cut(s)	1749
Bpu14I	TTCGAA	2 cut(s)	558, 1412
BpuEI	CTTGAG	2 cut(s)	1754, 2405
BpuMI	CCSGG	2 cut(s)	119, 1535
BsaAI	YACGTR	1 cut(s)	1106
BsaHI	GRCGYC	1 cut(s)	45
BsaI	GGTCTC	1 cut(s)	1118
BsaJI	CCNNGG	1 cut(s)	1261
BsaWI	WCCGGW	3 cut(s)	355, 1827, 2500
BsaXI	ACNNNNNCTCC	6 cut(s)	797, 827, 2088, 2118, 2451, 2481
Bsc4I	CCNNNNNNNGG	4 cut(s)	179, 1540, 2307, 2344
Bse118I	RCCGGY	1 cut(s)	2560
Bse1I	ACTGG	8 cut(s)	254, 742, 748, 1065, 1741, 2349, 2402, 2507
Bse21I	CCTNAGG	1 cut(s)	642
Bse3DI	GCAATG	2 cut(s)	956, 2064
BseAI	TCCGGA	3 cut(s)	355, 1827, 2500
BseBI	CCWGG	2 cut(s)	978, 1405
BseDI	CCNNGG	1 cut(s)	1261
BseGI	GGATG	6 cut(s)	483, 706, 1277, 1286, 1971, 2371
BseLI	CCNNNNNNNGG	4 cut(s)	179, 1540, 2307, 2344
BseMI	GCAATG	2 cut(s)	956, 2064
BseMII	CTCAG	7 cut(s)	633, 1247, 1506, 1575, 1740, 1856, 2417
BseNI	ACTGG	8 cut(s)	254, 742, 748, 1065, 1741, 2349, 2402, 2507
BseRI	GAGGAG	3 cut(s)	821, 1869, 2323
BseXI	GCAGC	8 cut(s)	567, 1095, 1416, 1805, 1840, 2328, 2375, 2390
BsgI	GTGCAG	2 cut(s)	22, 975
Bsh1236I	CGCG	1 cut(s)	212
BshFI	GGCC	2 cut(s)	1259, 2449
BsiHKAI	GWGCWC	1 cut(s)	1843
BsiSI	CCGG	7 cut(s)	118, 356, 1534, 1828, 2375, 2501, 2561
BslFI	GGGAC	3 cut(s)	911, 1742, 2319
BslI	CCNNNNNNNGG	4 cut(s)	179, 1540, 2307, 2344
BsmAI	GTCTC	9 cut(s)	98, 442, 1118, 1499, 1671, 1772, 2110, 2128, 2282
BsmBI	CGTCTC	2 cut(s)	98, 2110
BsmFI	GGGAC	3 cut(s)	911, 1742, 2319
BsnI	GGCC	2 cut(s)	1259, 2449
Bso31I	GGTCTC	1 cut(s)	1118
Bsp119I	TTCGAA	2 cut(s)	558, 1412
Bsp1286I	GDGCHC	1 cut(s)	1843
Bsp13I	TCCGGA	3 cut(s)	355, 1827, 2500
BspACI	CCGC	8 cut(s)	75, 534, 1087, 1540, 1796, 1868, 1943, 2085
BspANI	GGCC	2 cut(s)	1259, 2449
BspCNI	CTCAG	7 cut(s)	634, 1246, 1505, 1576, 1741, 1855, 2418
BspEI	TCCGGA	3 cut(s)	355, 1827, 2500
BspFNI	CGCG	1 cut(s)	212
BspMAI	CTGCAG	5 cut(s)	500, 585, 1434, 1674, 1858
BspMI	ACCTGC	2 cut(s)	574, 1567
BspPI	GGATC	8 cut(s)	544, 1066, 2219, 2232, 2487, 2498, 2500, 2511
BspQI	GCTCTTC	1 cut(s)	984
BspT104I	TTCGAA	2 cut(s)	558, 1412
BspTNI	GGTCTC	1 cut(s)	1118
BsrBI	CCGCTC	1 cut(s)	1089
BsrDI	GCAATG	2 cut(s)	956, 2064
BsrFI	RCCGGY	1 cut(s)	2560
BsrI	ACTGG	8 cut(s)	254, 742, 748, 1065, 1741, 2349, 2402, 2507
BssAI	RCCGGY	1 cut(s)	2560
BssECI	CCNNGG	1 cut(s)	1261
BssNI	GRCGYC	1 cut(s)	45
BssT1I	CCWWGG	1 cut(s)	1261
Bst2UI	CCWGG	2 cut(s)	978, 1405
Bst6I	CTCTTC	2 cut(s)	984, 1005
BstACI	GRCGYC	1 cut(s)	45
BstAPI	GCANNNNNTGC	1 cut(s)	2384
BstBAI	YACGTR	1 cut(s)	1106
BstBI	TTCGAA	2 cut(s)	558, 1412
BstC8I	GCNNGC	5 cut(s)	115, 1180, 1531, 1564, 1683
BstF5I	GGATG	6 cut(s)	483, 706, 1277, 1286, 1971, 2371
BstFNI	CGCG	1 cut(s)	212
BstHHI	GCGC	1 cut(s)	212
BstMAI	GTCTC	9 cut(s)	98, 442, 1118, 1499, 1671, 1772, 2110, 2128, 2282
BstMWI	GCNNNNNNNGC	7 cut(s)	665, 1095, 1616, 1678, 1793, 1900, 2384
BstNI	CCWGG	2 cut(s)	978, 1405
BstSCI	CCNGG	4 cut(s)	117, 976, 1403, 1533
BstSFI	CTRYAG	7 cut(s)	496, 581, 793, 1430, 1649, 1670, 1854
BstUI	CGCG	1 cut(s)	212
BstV1I	GCAGC	8 cut(s)	567, 1095, 1416, 1805, 1840, 2328, 2375, 2390
BstV2I	GAAGAC	4 cut(s)	625, 1766, 1803, 1853
BstX2I	RGATCY	6 cut(s)	536, 1058, 1495, 2224, 2492, 2503
BstXI	CCANNNNNNTGG	1 cut(s)	2409
BstYI	RGATCY	6 cut(s)	536, 1058, 1495, 2224, 2492, 2503
Bsu36I	CCTNAGG	1 cut(s)	642
BsuRI	GGCC	2 cut(s)	1259, 2449
BtgZI	GCGATG	1 cut(s)	1281
BtsCI	GGATG	6 cut(s)	483, 706, 1277, 1286, 1971, 2371
BtsI	GCAGTG	2 cut(s)	1093, 1189
BveI	ACCTGC	2 cut(s)	574, 1567
Cac8I	GCNNGC	5 cut(s)	115, 1180, 1531, 1564, 1683
CaiI	CAGNNNCTG	1 cut(s)	1669
CfoI	GCGC	1 cut(s)	212
Cfr10I	RCCGGY	1 cut(s)	2560
Cfr13I	GGNCC	7 cut(s)	358, 586, 898, 1258, 1556, 1729, 2576
CseI	GACGC	1 cut(s)	201
Csp6I	GTAC	4 cut(s)	150, 421, 768, 1597
CspCI	CAANNNNNGTGG	2 cut(s)	102, 137
CviAII	CATG	9 cut(s)	237, 401, 629, 1625, 1921, 2161, 2326, 2434, 2489
CviQI	GTAC	4 cut(s)	150, 421, 768, 1597
DraI	TTTAAA	1 cut(s)	411
DrdI	GACNNNNNNGTC	1 cut(s)	905
DriI	GACNNNNNGTC	1 cut(s)	1152
DseDI	GACNNNNNNGTC	1 cut(s)	905
Eam1104I	CTCTTC	2 cut(s)	984, 1005
Eam1105I	GACNNNNNGTC	1 cut(s)	1152
EarI	CTCTTC	2 cut(s)	984, 1005
Ecl136II	GAGCTC	1 cut(s)	1841
Eco130I	CCWWGG	1 cut(s)	1261
Eco24I	GRGCYC	1 cut(s)	1843
Eco31I	GGTCTC	1 cut(s)	1118
Eco47I	GGWCC	6 cut(s)	358, 586, 898, 1556, 1729, 2576
Eco53kI	GAGCTC	1 cut(s)	1841
Eco57I	CTGAAG	4 cut(s)	619, 1244, 1797, 1965
Eco81I	CCTNAGG	1 cut(s)	642
EcoICRI	GAGCTC	1 cut(s)	1841
EcoO109I	RGGNCCY	3 cut(s)	586, 898, 1729
EcoRI	GAATTC	3 cut(s)	346, 554, 1408
EcoRII	CCWGG	2 cut(s)	976, 1403
EcoT14I	CCWWGG	1 cut(s)	1261
EcoT38I	GRGCYC	1 cut(s)	1843
ErhI	CCWWGG	1 cut(s)	1261
Esp3I	CGTCTC	2 cut(s)	98, 2110
FaeI	CATG	9 cut(s)	240, 404, 632, 1628, 1924, 2164, 2329, 2437, 2492
FalI	AAGNNNNNCTT	2 cut(s)	1882, 1914
FaqI	GGGAC	3 cut(s)	911, 1742, 2319
FatI	CATG	9 cut(s)	236, 400, 628, 1624, 1920, 2160, 2325, 2433, 2488
FauI	CCCGC	2 cut(s)	1533, 2092
FauNDI	CATATG	1 cut(s)	1425
FbaI	TGATCA	1 cut(s)	325
FblI	GTMKAC	3 cut(s)	177, 505, 1170
Fnu4HI	GCNGC	9 cut(s)	581, 1084, 1087, 1430, 1794, 1854, 2317, 2364, 2379
FokI	GGATG	6 cut(s)	470, 713, 1264, 1273, 1958, 2358
FriOI	GRGCYC	1 cut(s)	1843
Fsp4HI	GCNGC	9 cut(s)	581, 1084, 1087, 1430, 1794, 1854, 2317, 2364, 2379
FspBI	CTAG	8 cut(s)	101, 425, 569, 1442, 1782, 1962, 2018, 2367
GlaI	GCGC	1 cut(s)	211
GluI	GCNGC	9 cut(s)	581, 1084, 1087, 1430, 1794, 1854, 2317, 2364, 2379
GsuI	CTGGAG	4 cut(s)	138, 824, 893, 2090
HaeIII	GGCC	2 cut(s)	1259, 2449
HapII	CCGG	7 cut(s)	118, 356, 1534, 1828, 2375, 2501, 2561
HgaI	GACGC	1 cut(s)	201
HhaI	GCGC	1 cut(s)	212
Hin1I	GRCGYC	1 cut(s)	45
Hin1II	CATG	9 cut(s)	240, 404, 632, 1628, 1924, 2164, 2329, 2437, 2492
Hin6I	GCGC	1 cut(s)	210
HinP1I	GCGC	1 cut(s)	210
HincII	GTYRAC	4 cut(s)	178, 790, 1157, 1987
HindII	GTYRAC	4 cut(s)	178, 790, 1157, 1987
HindIII	AAGCTT	1 cut(s)	1415
HpaI	GTTAAC	1 cut(s)	790
HpaII	CCGG	7 cut(s)	118, 356, 1534, 1828, 2375, 2501, 2561
HphI	GGTGA	3 cut(s)	212, 1226, 2550
Hpy99I	CGWCG	4 cut(s)	47, 179, 182, 217
HpyAV	CCTTC	8 cut(s)	81, 221, 260, 372, 1111, 1205, 1219, 2439
HpyCH4IV	ACGT	3 cut(s)	45, 1105, 1189
HpyF10VI	GCNNNNNNNGC	7 cut(s)	665, 1095, 1616, 1678, 1793, 1900, 2384
HpySE526I	ACGT	3 cut(s)	45, 1105, 1189
Hsp92I	GRCGYC	1 cut(s)	45
Hsp92II	CATG	9 cut(s)	240, 404, 632, 1628, 1924, 2164, 2329, 2437, 2492
HspAI	GCGC	1 cut(s)	210
Kpn2I	TCCGGA	3 cut(s)	355, 1827, 2500
Ksp22I	TGATCA	1 cut(s)	325
KspAI	GTTAAC	1 cut(s)	790
LguI	GCTCTTC	1 cut(s)	984
LmnI	GCTCC	2 cut(s)	1838, 1882
Lsp1109I	GCAGC	8 cut(s)	567, 1095, 1416, 1805, 1840, 2328, 2375, 2390
LweI	GCATC	4 cut(s)	1295, 1826, 2253, 2380
MaeI	CTAG	8 cut(s)	101, 425, 569, 1442, 1782, 1962, 2018, 2367
MaeII	ACGT	3 cut(s)	45, 1105, 1189
MaeIII	GTNAC	5 cut(s)	685, 1101, 1108, 2235, 2457
MbiI	CCGCTC	1 cut(s)	1089
MflI	RGATCY	6 cut(s)	536, 1058, 1495, 2224, 2492, 2503
MhlI	GDGCHC	1 cut(s)	1843
MlyI	GAGTC	5 cut(s)	440, 793, 1162, 2130, 2422
MmeI	TCCRAC	5 cut(s)	678, 918, 1036, 1938, 2259
MroI	TCCGGA	3 cut(s)	355, 1827, 2500
MroXI	GAANNNNTTC	1 cut(s)	1909
MseI	TTAA	6 cut(s)	93, 410, 474, 789, 2196, 2584
MspA1I	CMGCKG	1 cut(s)	1796
MspI	CCGG	7 cut(s)	118, 356, 1534, 1828, 2375, 2501, 2561
MspR9I	CCNGG	4 cut(s)	119, 978, 1405, 1535
MvaI	CCWGG	2 cut(s)	978, 1405
MvnI	CGCG	1 cut(s)	212
MwoI	GCNNNNNNNGC	7 cut(s)	665, 1095, 1616, 1678, 1793, 1900, 2384
NciI	CCSGG	2 cut(s)	119, 1535
NdeI	CATATG	1 cut(s)	1425
NlaIII	CATG	9 cut(s)	240, 404, 632, 1628, 1924, 2164, 2329, 2437, 2492
NmeAIII	GCCGAG	1 cut(s)	1235
NmuCI	GTSAC	3 cut(s)	685, 1108, 2235
NspV	TTCGAA	2 cut(s)	558, 1412
PciSI	GCTCTTC	1 cut(s)	984
PdmI	GAANNNNTTC	1 cut(s)	1909
PfeI	GAWTC	7 cut(s)	185, 514, 560, 599, 1229, 1723, 2058
PflFI	GACNNNGTC	1 cut(s)	629
PfoI	TCCNGGA	1 cut(s)	976
PkrI	GCNGC	9 cut(s)	582, 1085, 1088, 1431, 1795, 1855, 2318, 2365, 2380
PleI	GAGTC	5 cut(s)	439, 793, 1161, 2129, 2421
PpsI	GAGTC	5 cut(s)	439, 793, 1161, 2129, 2421
Ppu21I	YACGTR	1 cut(s)	1106
PpuMI	RGGWCCY	3 cut(s)	586, 898, 1729
PsiI	TTATAA	2 cut(s)	882, 1241
Psp124BI	GAGCTC	1 cut(s)	1843
Psp5II	RGGWCCY	3 cut(s)	586, 898, 1729
Psp6I	CCWGG	2 cut(s)	976, 1403
PspGI	CCWGG	2 cut(s)	976, 1403
PspPI	GGNCC	7 cut(s)	358, 586, 898, 1258, 1556, 1729, 2576
PspPPI	RGGWCCY	3 cut(s)	586, 898, 1729
PstI	CTGCAG	5 cut(s)	500, 585, 1434, 1674, 1858
PstNI	CAGNNNCTG	1 cut(s)	1669
PsuI	RGATCY	6 cut(s)	536, 1058, 1495, 2224, 2492, 2503
PsyI	GACNNNGTC	1 cut(s)	629
RsaI	GTAC	4 cut(s)	151, 422, 769, 1598
RsaNI	GTAC	4 cut(s)	150, 421, 768, 1597
SacI	GAGCTC	1 cut(s)	1843
SalI	GTCGAC	1 cut(s)	176
SapI	GCTCTTC	1 cut(s)	984
SaqAI	TTAA	6 cut(s)	93, 410, 474, 789, 2196, 2584
SatI	GCNGC	9 cut(s)	581, 1084, 1087, 1430, 1794, 1854, 2317, 2364, 2379
Sau96I	GGNCC	7 cut(s)	358, 586, 898, 1258, 1556, 1729, 2576
SchI	GAGTC	5 cut(s)	440, 793, 1162, 2130, 2422
ScrFI	CCNGG	4 cut(s)	119, 978, 1405, 1535
SduI	GDGCHC	1 cut(s)	1843
SfaNI	GCATC	4 cut(s)	1295, 1826, 2253, 2380
SfcI	CTRYAG	7 cut(s)	496, 581, 793, 1430, 1649, 1670, 1854
SfuI	TTCGAA	2 cut(s)	558, 1412
SgrDI	CGTCGACG	1 cut(s)	176
SinI	GGWCC	6 cut(s)	358, 586, 898, 1556, 1729, 2576
SmlI	CTYRAG	2 cut(s)	1733, 2420
SmoI	CTYRAG	2 cut(s)	1733, 2420
SsiI	CCGC	8 cut(s)	75, 534, 1087, 1540, 1796, 1868, 1943, 2085
SspMI	CTAG	8 cut(s)	101, 425, 569, 1442, 1782, 1962, 2018, 2367
SstI	GAGCTC	1 cut(s)	1843
StyD4I	CCNGG	4 cut(s)	117, 976, 1403, 1533
StyI	CCWWGG	1 cut(s)	1261
TaiI	ACGT	3 cut(s)	48, 1108, 1192
TaqI	TCGA	7 cut(s)	138, 177, 215, 558, 909, 1412, 2246
TatI	WGTACW	2 cut(s)	149, 1596
TauI	GCSGC	1 cut(s)	1089
TfiI	GAWTC	7 cut(s)	185, 514, 560, 599, 1229, 1723, 2058
Tru1I	TTAA	6 cut(s)	93, 410, 474, 789, 2196, 2584
Tru9I	TTAA	6 cut(s)	93, 410, 474, 789, 2196, 2584
TseFI	GTSAC	3 cut(s)	685, 1108, 2235
TseI	GCWGC	8 cut(s)	580, 1083, 1429, 1793, 1853, 2316, 2363, 2378
Tsp45I	GTSAC	3 cut(s)	685, 1108, 2235
TspDTI	ATGAA	6 cut(s)	417, 474, 1506, 1909, 2314, 2450
TspGWI	ACGGA	6 cut(s)	195, 234, 535, 975, 2004, 2410
Tth111I	GACNNNGTC	1 cut(s)	629
VpaK11BI	GGWCC	6 cut(s)	358, 586, 898, 1556, 1729, 2576
XapI	RAATTY	8 cut(s)	288, 346, 525, 554, 1408, 1908, 2043, 2549
XbaI	TCTAGA	1 cut(s)	1961
XcmI	CCANNNNNNNNNTGG	1 cut(s)	934
XmiI	GTMKAC	3 cut(s)	177, 505, 1170
XmnI	GAANNNNTTC	1 cut(s)	1909
XspI	CTAG	8 cut(s)	101, 425, 569, 1442, 1782, 1962, 2018, 2367
ZraI	GACGTC	1 cut(s)	46

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.