Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0008598

Physical Location & Seq

Genomic Coordinates Forward (+)

21977847 .. 21982256

Gene Structure 4410 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g20490.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2586 bp

ATGTTGCGTCATAGACGACGTGAAACTAAGCATCCCTTCAATTCAAACTTCAAAGCAATCAAAACCCTACCCTCACAAATCCCTCCGGTCCTGAATGCAAAACTCAAAACGCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACACTCCAGAAGTCGCATTCTTGCTCCGATTCGAATCCGTGGAAGATCAAACGGTGGAAATGTTCCACGAAAACTGCACCGACGTCGTTCCAGATCACTACTCCAAGCCTTCCGCAACCTCCGAAGCAGTTAAAACCCTAGAGACGATAGCAAGCCAGGTGGACAGTGATGTGAGCGACAAAGAATGTACTCCAGACATTACTACCAAACCCGTCGACGGAGATTCTGATTTGATTGGTGAGAGTAGCGCGTCGAACGGAACAGAAGGTTCTCCATGTCAAAGGTTCCTTCCAGTTTTGCAGAAGATCACTGATTTGACAACCAAAATTCAGGATTTGAAGAAAGAGCATACTGTCTTGTCTGATCAAGTCAAGCTTACAACGAATTCCTTTCCAGACCCTTCAGTTCTAAACACCATTCAGCTGCTAAGTATGGAACATGAACTTTTAAAGAAGAAGTACCTAGATGAGTTGTCCGAGCGAAAGAGACTTTACAATGAAGTGATTGAGCTTAAAGGCAACATCCGAGTTTTCTGCAGATGTAGACCTTTGAATCAAACTGAAATTTCAAGTGGATCTGGTTTCGTTGTTGAATTCGAATCCTCCCTAGATAACGAGCTGCATGTCATTTGTTCTGATTCCTCGAAAAAGCAGTTCAAGTTTGACCATGTCTTCAGACCTGAGGACGACCAAGAGGCTGTTTTTTCCCAAACGAAGCCTATTGTGACTTCGGTGTTGGATGGGTATAATGTCTGCATTTTTTCCTATGGACAAACTGGAACTGGCAAGACCTTTACGATGGAGGGTACTCCTGAAAACAGAGGTGTTAACTACAGGACTCTGGAGGAGTTGTTTCGGATTTCAAAAGACAGAGGTGGCTTTATGAGATATGAGTTATGTGTTAGTATGCTGGAGGTTTATAATGAGAAGATTAGGGACCTCTTGGTCAATAACACTAATCAACCCACAAAGAAGTTGGAAATAAAGCAATGTGCAGACGGAACCTTAGATGTTCCAGGACTTGTCGAAGAGCGTGTTTATGGCTTTGAAGAGATGTGGGAACTGCTAAAGTCTGGAAGCCAAGCAAGGTCTGTTGGATCTACCAGTGCTAATGAGCAGAGCAGCCGCTCTCACTGCTTGTTGCGGGTGACAGTGAAGGGGGAGAACTTGATAAATGGGCAAAAGACAAGGAGTCAACTGTGGCTCGTAGACTTGGCTGGCAGTGAACGTGTGGGGAGGATTGATGTTGATGGTGAAAGACTGAAGGAATCTCAGTTTATAAATAAATCTCTCTCGGCCCTTGGCGATGTTATCTCATCCCTTGCATCCAAGACAGCCCACATTCCTTACAGGAATTCGAAGCTTACTCATATGCTGCAGAGTTCTCTAGGGGGAGATTGCAAAACCTTGATGTTTGTCCAAATCAGCCCAAGTGCCTCAGATCTTGGAGAGACACTGTGTTCATTGAACTTTGCCAGCCGGGTGCGGGGAGTAGAGAGTGGACCTGCTCGCAAACAAGCAGACCTCACTGAGCTGTTCAAGTACAAACAAATGGCTGAAAAGGCTAAACATGACGAGAAAGAAACAAAGAAACTACAGGATTGCATACAGTCTCTGCAGTTGAGGCTTGCTTCAAGGGAACACATCTGCAGAAATCTACAAGAAAAGAATCGAGACCTTGAGAACCAGTTGGCTGAGGAAAGGAAGACCAGACTGAAACAGGAGACTAGAGCTTTCGCAGCGGTTTCTCATCAGTCTTCAGCATCATCATTCCGAAAACAAGGAGCTCAGAAGACTGCTGCAGAGAAGAAACCGCCATTGGCTCCTCCAAGATCAAGGCTACCTTTGCGAAGAATTTCCAACTTCATGCCCCCACCATCTGCTTTACCGCCCAAAAAAACCATCTCTAGAACTCCCGTCCCATCTTCAGTGGATGGAAAAGAAAACATCTCCACAACAGCACTAGCAGGAAGAAACCAGAAAAGCCAAATTTTACCGAGAAGAATCTCCATTGCTGTCAGACCTCCACCCGCAACAACACAACAGGTTCTCCAGCCTAAGAGACGAGTCTCTATTGCTACATATCGCCCTGACCCGAACTCTCACATGACAACACCCCTCCACACCTCTGCCTCTCTATTTAACAGTGGCAGACTGTCGTTCGCAAGGGATCCACGAAAGGCACGGTATTCGAGGCTGTTCTCCCCGATGCCTGAATTGAAGTCGGAAGCAGAGACAACACCAACCACAATGAGGAGAAGCAGCAAGTTCATGGGTAGTCCCCCGACACTGGCTGGTTCAGTCAGGGCAGCGAGGCATCCGGCAGCAGTTGCACTACAACGGAAACCAGTAATCTGGAGTCCACTCAAGCTGAGAGGCATGAAAAACAGAAGGCCATCACTGTTACTGCCTCTCCGAGGTTCGAATGACATGCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin Microtub_bd

Protein Analysis

862

Amino Acids

96.41

Weight (kDa)

9.5

Isoelectric Point (pI)

47.28

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008598)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27550 AT5G27550
fragaria_vesca	FvH4_6g05830 FvH4_6g05830 FvH4_6g05830 FvH4_6g05830 FvH4_6g05830
malus_domestica	MD04G1205300.v1.1 MD12G1219400.v1.1
prunus_persica	Prupe.6G326000_v2.0.a1 Prupe.6G326000_v2.0.a1
pyrus_communis	pycom04g18160 pycom12g20490
rosa_chinensis	RchiOBHm_Chr3g0454741
rosa_laevigata	RLG00000025419
rosa_multiflora	Rmu_sc0003140.1_g000019
rosa_roxburghii	Rroxscaffold_6G00424800
rosa_rugosa	Rorug03G0002100
rosa_samantha	Rh3AG062100 Rh3BG064000 Rh3CG062900 Rh3DG063700
rosa_wichuraiana	Rw3G004830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1068, 1427
AasI	GACNNNNNNGTC	1 cut(s)	1091
AatII	GACGTC	1 cut(s)	234
Acc36I	ACCTGC	1 cut(s)	1660
AccBSI	CCGCTC	1 cut(s)	1275
AccI	GTMKAC	3 cut(s)	363, 691, 1356
AccII	CGCG	1 cut(s)	398
AciI	CCGC	9 cut(s)	261, 1273, 1291, 1633, 1889, 1961, 2036, 2178, 2581
AclWI	GGATC	4 cut(s)	730, 1252, 2312, 2325
AcsI	RAATTY	7 cut(s)	474, 532, 711, 740, 1501, 2001, 2136
AcuI	CTGAAG	5 cut(s)	534, 805, 1430, 1890, 2058
AcyI	GRCGYC	1 cut(s)	231
AfaI	GTAC	4 cut(s)	337, 608, 955, 1691
AfiI	CCNNNNNNNGG	5 cut(s)	365, 1633, 2400, 2437, 2564
AflIII	ACRYGT	1 cut(s)	1375
AhdI	GACNNNNNGTC	1 cut(s)	1338
AjiI	CACGTC	1 cut(s)	20
AjnI	CCWGG	2 cut(s)	303, 1162
AjuI	GAANNNNNNNTTGG	2 cut(s)	1949, 1981
AluBI	AGCT	9 cut(s)	523, 571, 658, 766, 1510, 1681, 1880, 1934, 2518
AluI	AGCT	9 cut(s)	523, 571, 658, 766, 1510, 1681, 1880, 1934, 2518
Alw21I	GWGCWC	1 cut(s)	1936
Alw26I	GTCTC	9 cut(s)	284, 628, 1592, 1764, 1815, 1865, 2203, 2221, 2375
AlwI	GGATC	4 cut(s)	730, 1252, 2312, 2325
AlwNI	CAGNNNCTG	1 cut(s)	1762
AoxI	GGCC	2 cut(s)	1443, 2540
ApeKI	GCWGC	9 cut(s)	571, 766, 1269, 1522, 1886, 1946, 2409, 2456, 2471
ApoI	RAATTY	7 cut(s)	474, 532, 711, 740, 1501, 2001, 2136
Asp700I	GAANNNNTTC	1 cut(s)	2002
AspLEI	GCGC	1 cut(s)	398
AspS9I	GGNCC	4 cut(s)	88, 1084, 1444, 1649
AsuC2I	CCSGG	1 cut(s)	1628
AsuHPI	GGTGA	3 cut(s)	398, 1306, 1412
AsuII	TTCGAA	4 cut(s)	180, 744, 1505, 2570
AvaII	GGWCC	3 cut(s)	88, 1084, 1649
AxyI	CCTNAGG	1 cut(s)	828
BamHI	GGATCC	1 cut(s)	2317
BanII	GRGCYC	1 cut(s)	1936
BbsI	GAAGAC	4 cut(s)	811, 1859, 1896, 1946
Bbv12I	GWGCWC	1 cut(s)	1936
BbvCI	CCTCAGC	1 cut(s)	1842
BbvI	GCAGC	9 cut(s)	558, 753, 1281, 1509, 1898, 1933, 2421, 2468, 2483
BccI	CCATC	8 cut(s)	881, 940, 1391, 2032, 2057, 2075, 2077, 2551
BcgI	CGANNNNNNTGC	7 cut(s)	214, 248, 663, 697, 2319, 2353, 2560
BciT130I	CCWGG	2 cut(s)	305, 1164
BclI	TGATCA	1 cut(s)	511
BcnI	CCSGG	1 cut(s)	1628
BcoDI	GTCTC	9 cut(s)	284, 628, 1592, 1764, 1815, 1865, 2203, 2221, 2375
BfaI	CTAG	7 cut(s)	287, 611, 755, 1535, 1875, 2055, 2111
BfmI	CTRYAG	7 cut(s)	682, 979, 1523, 1742, 1763, 1795, 1947
BfuAI	ACCTGC	1 cut(s)	1660
BglII	AGATCT	1 cut(s)	1588
Bme1390I	CCNGG	3 cut(s)	305, 1164, 1628
Bme18I	GGWCC	3 cut(s)	88, 1084, 1649
BmeRI	GACNNNNNGTC	1 cut(s)	1338
BmgBI	CACGTC	1 cut(s)	20
BmgT120I	GGNCC	4 cut(s)	88, 1084, 1444, 1649
BmiI	GGNNCC	5 cut(s)	434, 1085, 1150, 1971, 2319
BmrFI	CCNGG	3 cut(s)	305, 1164, 1628
BmsI	GCATC	5 cut(s)	40, 1481, 1919, 2346, 2473
BpiI	GAAGAC	4 cut(s)	811, 1859, 1896, 1946
BpmI	CTGGAG	6 cut(s)	138, 324, 1010, 1079, 2183, 2524
Bpu10I	CCTNAGC	1 cut(s)	1842
Bpu14I	TTCGAA	4 cut(s)	180, 744, 1505, 2570
BpuEI	CTTGAG	2 cut(s)	1847, 2498
BpuMI	CCSGG	1 cut(s)	1628
BsaBI	GATNNNNATC	1 cut(s)	181
BsaHI	GRCGYC	1 cut(s)	231
BsaI	GGTCTC	1 cut(s)	1815
BsaJI	CCNNGG	3 cut(s)	186, 1447, 2563
BsaWI	WCCGGW	1 cut(s)	85
BsaXI	ACNNNNNCTCC	6 cut(s)	983, 1013, 2181, 2211, 2544, 2574
Bsc4I	CCNNNNNNNGG	5 cut(s)	365, 1633, 2400, 2437, 2564
Bse1I	ACTGG	7 cut(s)	440, 928, 934, 1251, 1834, 2442, 2495
Bse21I	CCTNAGG	1 cut(s)	828
Bse3DI	GCAATG	2 cut(s)	1142, 2157
Bse8I	GATNNNNATC	1 cut(s)	181
BseBI	CCWGG	2 cut(s)	305, 1164
BseDI	CCNNGG	3 cut(s)	186, 1447, 2563
BseGI	GGATG	7 cut(s)	31, 669, 892, 1463, 1472, 2086, 2464
BseJI	GATNNNNATC	1 cut(s)	181
BseLI	CCNNNNNNNGG	5 cut(s)	365, 1633, 2400, 2437, 2564
BseMI	GCAATG	2 cut(s)	1142, 2157
BseMII	CTCAG	7 cut(s)	819, 1433, 1599, 1668, 1833, 1949, 2510
BseNI	ACTGG	7 cut(s)	440, 928, 934, 1251, 1834, 2442, 2495
BseRI	GAGGAG	3 cut(s)	1007, 1962, 2416
BseXI	GCAGC	9 cut(s)	558, 753, 1281, 1509, 1898, 1933, 2421, 2468, 2483
BsgI	GTGCAG	2 cut(s)	208, 1161
Bsh1236I	CGCG	1 cut(s)	398
BshFI	GGCC	2 cut(s)	1445, 2542
BsiHKAI	GWGCWC	1 cut(s)	1936
BsiSI	CCGG	3 cut(s)	86, 1627, 2468
BslFI	GGGAC	3 cut(s)	1097, 2051, 2412
BslI	CCNNNNNNNGG	5 cut(s)	365, 1633, 2400, 2437, 2564
BsmAI	GTCTC	9 cut(s)	284, 628, 1592, 1764, 1815, 1865, 2203, 2221, 2375
BsmBI	CGTCTC	2 cut(s)	284, 2203
BsmFI	GGGAC	3 cut(s)	1097, 2051, 2412
BsmI	GAATGC	2 cut(s)	100, 164
BsnI	GGCC	2 cut(s)	1445, 2542
Bso31I	GGTCTC	1 cut(s)	1815
Bsp119I	TTCGAA	4 cut(s)	180, 744, 1505, 2570
Bsp1286I	GDGCHC	1 cut(s)	1936
Bsp143I	GATC	9 cut(s)	193, 241, 453, 511, 722, 1244, 1588, 1979, 2317
BspACI	CCGC	9 cut(s)	261, 1273, 1291, 1633, 1889, 1961, 2036, 2178, 2581
BspANI	GGCC	2 cut(s)	1445, 2542
BspCNI	CTCAG	7 cut(s)	820, 1432, 1598, 1669, 1834, 1948, 2511
BspFNI	CGCG	1 cut(s)	398
BspLI	GGNNCC	5 cut(s)	434, 1085, 1150, 1971, 2319
BspMAI	CTGCAG	5 cut(s)	686, 1527, 1767, 1799, 1951
BspMI	ACCTGC	1 cut(s)	1660
BspPI	GGATC	4 cut(s)	730, 1252, 2312, 2325
BspQI	GCTCTTC	1 cut(s)	1170
BspT104I	TTCGAA	4 cut(s)	180, 744, 1505, 2570
BspTNI	GGTCTC	1 cut(s)	1815
BsrBI	CCGCTC	1 cut(s)	1275
BsrDI	GCAATG	2 cut(s)	1142, 2157
BsrI	ACTGG	7 cut(s)	440, 928, 934, 1251, 1834, 2442, 2495
BssECI	CCNNGG	3 cut(s)	186, 1447, 2563
BssMI	GATC	9 cut(s)	193, 241, 453, 511, 722, 1244, 1588, 1979, 2317
BssNI	GRCGYC	1 cut(s)	231
BssT1I	CCWWGG	1 cut(s)	1447
Bst2UI	CCWGG	2 cut(s)	305, 1164
Bst6I	CTCTTC	2 cut(s)	1170, 1191
BstACI	GRCGYC	1 cut(s)	231
BstAPI	GCANNNNNTGC	1 cut(s)	2477
BstBI	TTCGAA	4 cut(s)	180, 744, 1505, 2570
BstC8I	GCNNGC	5 cut(s)	301, 1366, 1624, 1657, 1776
BstDSI	CCRYGG	1 cut(s)	186
BstENI	CCTNNNNNAGG	1 cut(s)	2562
BstF5I	GGATG	7 cut(s)	31, 669, 892, 1463, 1472, 2086, 2464
BstFNI	CGCG	1 cut(s)	398
BstHHI	GCGC	1 cut(s)	398
BstKTI	GATC	9 cut(s)	196, 244, 456, 514, 725, 1247, 1591, 1982, 2320
BstMAI	GTCTC	9 cut(s)	284, 628, 1592, 1764, 1815, 1865, 2203, 2221, 2375
BstMBI	GATC	9 cut(s)	193, 241, 453, 511, 722, 1244, 1588, 1979, 2317
BstMWI	GCNNNNNNNGC	6 cut(s)	1281, 1709, 1771, 1886, 1993, 2477
BstNI	CCWGG	2 cut(s)	305, 1164
BstNSI	RCATGY	2 cut(s)	773, 2581
BstSCI	CCNGG	3 cut(s)	303, 1162, 1626
BstSFI	CTRYAG	7 cut(s)	682, 979, 1523, 1742, 1763, 1795, 1947
BstUI	CGCG	1 cut(s)	398
BstV1I	GCAGC	9 cut(s)	558, 753, 1281, 1509, 1898, 1933, 2421, 2468, 2483
BstV2I	GAAGAC	4 cut(s)	811, 1859, 1896, 1946
BstX2I	RGATCY	4 cut(s)	722, 1244, 1588, 2317
BstXI	CCANNNNNNTGG	1 cut(s)	2502
BstYI	RGATCY	4 cut(s)	722, 1244, 1588, 2317
Bsu36I	CCTNAGG	1 cut(s)	828
BsuRI	GGCC	2 cut(s)	1445, 2542
BtgI	CCRYGG	1 cut(s)	186
BtgZI	GCGATG	1 cut(s)	1467
BtrI	CACGTC	1 cut(s)	20
BtsCI	GGATG	7 cut(s)	31, 669, 892, 1463, 1472, 2086, 2464
BtsI	GCAGTG	2 cut(s)	1279, 1375
BveI	ACCTGC	1 cut(s)	1660
Cac8I	GCNNGC	5 cut(s)	301, 1366, 1624, 1657, 1776
CaiI	CAGNNNCTG	1 cut(s)	1762
CfoI	GCGC	1 cut(s)	398
Cfr13I	GGNCC	4 cut(s)	88, 1084, 1444, 1649
CseI	GACGC	1 cut(s)	387
Csp6I	GTAC	4 cut(s)	336, 607, 954, 1690
CspCI	CAANNNNNGTGG	2 cut(s)	288, 323
CviQI	GTAC	4 cut(s)	336, 607, 954, 1690
DpnI	GATC	9 cut(s)	195, 243, 455, 513, 724, 1246, 1590, 1981, 2319
DpnII	GATC	9 cut(s)	193, 241, 453, 511, 722, 1244, 1588, 1979, 2317
DraI	TTTAAA	1 cut(s)	597
DrdI	GACNNNNNNGTC	1 cut(s)	1091
DriI	GACNNNNNGTC	1 cut(s)	1338
DseDI	GACNNNNNNGTC	1 cut(s)	1091
Eam1104I	CTCTTC	2 cut(s)	1170, 1191
Eam1105I	GACNNNNNGTC	1 cut(s)	1338
EarI	CTCTTC	2 cut(s)	1170, 1191
Ecl136II	GAGCTC	1 cut(s)	1934
Eco130I	CCWWGG	1 cut(s)	1447
Eco24I	GRGCYC	1 cut(s)	1936
Eco31I	GGTCTC	1 cut(s)	1815
Eco47I	GGWCC	3 cut(s)	88, 1084, 1649
Eco53kI	GAGCTC	1 cut(s)	1934
Eco57I	CTGAAG	5 cut(s)	534, 805, 1430, 1890, 2058
Eco81I	CCTNAGG	1 cut(s)	828
EcoICRI	GAGCTC	1 cut(s)	1934
EcoNI	CCTNNNNNAGG	1 cut(s)	2562
EcoO109I	RGGNCCY	1 cut(s)	1084
EcoRI	GAATTC	3 cut(s)	532, 740, 1501
EcoRII	CCWGG	2 cut(s)	303, 1162
EcoT14I	CCWWGG	1 cut(s)	1447
EcoT38I	GRGCYC	1 cut(s)	1936
ErhI	CCWWGG	1 cut(s)	1447
Esp3I	CGTCTC	2 cut(s)	284, 2203
FalI	AAGNNNNNCTT	6 cut(s)	20, 52, 507, 539, 1975, 2007
FaqI	GGGAC	3 cut(s)	1097, 2051, 2412
FauI	CCCGC	3 cut(s)	1284, 1626, 2185
FauNDI	CATATG	1 cut(s)	1518
FbaI	TGATCA	1 cut(s)	511
FblI	GTMKAC	3 cut(s)	363, 691, 1356
FokI	GGATG	7 cut(s)	18, 656, 899, 1450, 1459, 2093, 2451
FriOI	GRGCYC	1 cut(s)	1936
FspBI	CTAG	7 cut(s)	287, 611, 755, 1535, 1875, 2055, 2111
GlaI	GCGC	1 cut(s)	397
GsuI	CTGGAG	6 cut(s)	138, 324, 1010, 1079, 2183, 2524
HaeIII	GGCC	2 cut(s)	1445, 2542
HapII	CCGG	3 cut(s)	86, 1627, 2468
HgaI	GACGC	1 cut(s)	387
HhaI	GCGC	1 cut(s)	398
Hin1I	GRCGYC	1 cut(s)	231
Hin6I	GCGC	1 cut(s)	396
HinP1I	GCGC	1 cut(s)	396
HincII	GTYRAC	3 cut(s)	364, 976, 1343
HindII	GTYRAC	3 cut(s)	364, 976, 1343
HindIII	AAGCTT	2 cut(s)	521, 1508
HpaI	GTTAAC	1 cut(s)	976
HpaII	CCGG	3 cut(s)	86, 1627, 2468
HphI	GGTGA	3 cut(s)	398, 1306, 1412
Hpy166II	GTNNAC	9 cut(s)	310, 364, 692, 976, 1343, 1357, 1373, 1649, 2510
Hpy8I	GTNNAC	9 cut(s)	310, 364, 692, 976, 1343, 1357, 1373, 1649, 2510
Hpy99I	CGWCG	6 cut(s)	21, 233, 236, 365, 368, 403
HpyAV	CCTTC	8 cut(s)	46, 267, 407, 446, 558, 1297, 1405, 2532
HpyCH4IV	ACGT	3 cut(s)	19, 231, 1375
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1281, 1709, 1771, 1886, 1993, 2477
HpySE526I	ACGT	3 cut(s)	19, 231, 1375
Hsp92I	GRCGYC	1 cut(s)	231
HspAI	GCGC	1 cut(s)	396
Ksp22I	TGATCA	1 cut(s)	511
KspAI	GTTAAC	1 cut(s)	976
Kzo9I	GATC	9 cut(s)	193, 241, 453, 511, 722, 1244, 1588, 1979, 2317
LguI	GCTCTTC	1 cut(s)	1170
LmnI	GCTCC	4 cut(s)	117, 177, 1931, 1975
Lsp1109I	GCAGC	9 cut(s)	558, 753, 1281, 1509, 1898, 1933, 2421, 2468, 2483
LweI	GCATC	5 cut(s)	40, 1481, 1919, 2346, 2473
MaeI	CTAG	7 cut(s)	287, 611, 755, 1535, 1875, 2055, 2111
MaeII	ACGT	3 cut(s)	19, 231, 1375
MaeIII	GTNAC	3 cut(s)	871, 1294, 2550
MalI	GATC	9 cut(s)	195, 243, 455, 513, 724, 1246, 1590, 1981, 2319
MbiI	CCGCTC	1 cut(s)	1275
MboI	GATC	9 cut(s)	193, 241, 453, 511, 722, 1244, 1588, 1979, 2317
MflI	RGATCY	4 cut(s)	722, 1244, 1588, 2317
MhlI	GDGCHC	1 cut(s)	1936
MluCI	AATT	9 cut(s)	40, 474, 532, 711, 740, 1501, 2001, 2136, 2363
MlyI	GAGTC	4 cut(s)	979, 1348, 2223, 2515
MmeI	TCCRAC	5 cut(s)	864, 1104, 1222, 2031, 2352
MroXI	GAANNNNTTC	1 cut(s)	2002
MseI	TTAA	5 cut(s)	279, 596, 660, 975, 2289
MspA1I	CMGCKG	2 cut(s)	571, 1889
MspI	CCGG	3 cut(s)	86, 1627, 2468
MspR9I	CCNGG	3 cut(s)	305, 1164, 1628
Mva1269I	GAATGC	2 cut(s)	100, 164
MvaI	CCWGG	2 cut(s)	305, 1164
MvnI	CGCG	1 cut(s)	398
MwoI	GCNNNNNNNGC	6 cut(s)	1281, 1709, 1771, 1886, 1993, 2477
NciI	CCSGG	1 cut(s)	1628
NdeI	CATATG	1 cut(s)	1518
NdeII	GATC	9 cut(s)	193, 241, 453, 511, 722, 1244, 1588, 1979, 2317
NlaIV	GGNNCC	5 cut(s)	434, 1085, 1150, 1971, 2319
NmeAIII	GCCGAG	1 cut(s)	1421
NmuCI	GTSAC	2 cut(s)	871, 1294
NspI	RCATGY	2 cut(s)	773, 2581
NspV	TTCGAA	4 cut(s)	180, 744, 1505, 2570
PciSI	GCTCTTC	1 cut(s)	1170
PctI	GAATGC	2 cut(s)	100, 164
PdmI	GAANNNNTTC	1 cut(s)	2002
PfeI	GAWTC	9 cut(s)	177, 182, 371, 700, 746, 785, 1415, 1816, 2151
PflFI	GACNNNGTC	1 cut(s)	815
PfoI	TCCNGGA	1 cut(s)	1162
PleI	GAGTC	4 cut(s)	979, 1347, 2222, 2514
PpsI	GAGTC	4 cut(s)	979, 1347, 2222, 2514
PpuMI	RGGWCCY	1 cut(s)	1084
PsiI	TTATAA	2 cut(s)	1068, 1427
Psp124BI	GAGCTC	1 cut(s)	1936
Psp5II	RGGWCCY	1 cut(s)	1084
Psp6I	CCWGG	2 cut(s)	303, 1162
PspGI	CCWGG	2 cut(s)	303, 1162
PspN4I	GGNNCC	5 cut(s)	434, 1085, 1150, 1971, 2319
PspPI	GGNCC	4 cut(s)	88, 1084, 1444, 1649
PspPPI	RGGWCCY	1 cut(s)	1084
PstI	CTGCAG	5 cut(s)	686, 1527, 1767, 1799, 1951
PstNI	CAGNNNCTG	1 cut(s)	1762
PsuI	RGATCY	4 cut(s)	722, 1244, 1588, 2317
PsyI	GACNNNGTC	1 cut(s)	815
PvuII	CAGCTG	1 cut(s)	571
RsaI	GTAC	4 cut(s)	337, 608, 955, 1691
RsaNI	GTAC	4 cut(s)	336, 607, 954, 1690
SacI	GAGCTC	1 cut(s)	1936
SalI	GTCGAC	1 cut(s)	362
SapI	GCTCTTC	1 cut(s)	1170
SaqAI	TTAA	5 cut(s)	279, 596, 660, 975, 2289
Sau3AI	GATC	9 cut(s)	193, 241, 453, 511, 722, 1244, 1588, 1979, 2317
Sau96I	GGNCC	4 cut(s)	88, 1084, 1444, 1649
SchI	GAGTC	4 cut(s)	979, 1348, 2223, 2515
ScrFI	CCNGG	3 cut(s)	305, 1164, 1628
SduI	GDGCHC	1 cut(s)	1936
SfaNI	GCATC	5 cut(s)	40, 1481, 1919, 2346, 2473
SfcI	CTRYAG	7 cut(s)	682, 979, 1523, 1742, 1763, 1795, 1947
SfuI	TTCGAA	4 cut(s)	180, 744, 1505, 2570
SgrDI	CGTCGACG	1 cut(s)	362
SinI	GGWCC	3 cut(s)	88, 1084, 1649
SmlI	CTYRAG	2 cut(s)	1826, 2513
SmoI	CTYRAG	2 cut(s)	1826, 2513
Sse9I	AATT	9 cut(s)	40, 474, 532, 711, 740, 1501, 2001, 2136, 2363
SsiI	CCGC	9 cut(s)	261, 1273, 1291, 1633, 1889, 1961, 2036, 2178, 2581
SspMI	CTAG	7 cut(s)	287, 611, 755, 1535, 1875, 2055, 2111
SstI	GAGCTC	1 cut(s)	1936
StyD4I	CCNGG	3 cut(s)	303, 1162, 1626
StyI	CCWWGG	1 cut(s)	1447
TaiI	ACGT	3 cut(s)	22, 234, 1378
TasI	AATT	9 cut(s)	40, 474, 532, 711, 740, 1501, 2001, 2136, 2363
TatI	WGTACW	2 cut(s)	335, 1689
TauI	GCSGC	1 cut(s)	1275
TfiI	GAWTC	9 cut(s)	177, 182, 371, 700, 746, 785, 1415, 1816, 2151
Tru1I	TTAA	5 cut(s)	279, 596, 660, 975, 2289
Tru9I	TTAA	5 cut(s)	279, 596, 660, 975, 2289
TseFI	GTSAC	2 cut(s)	871, 1294
TseI	GCWGC	9 cut(s)	571, 766, 1269, 1522, 1886, 1946, 2409, 2456, 2471
Tsp45I	GTSAC	2 cut(s)	871, 1294
TspDTI	ATGAA	6 cut(s)	603, 660, 1599, 2002, 2407, 2543
TspGWI	ACGGA	5 cut(s)	175, 381, 420, 1161, 2503
Tth111I	GACNNNGTC	1 cut(s)	815
VpaK11BI	GGWCC	3 cut(s)	88, 1084, 1649
XagI	CCTNNNNNAGG	1 cut(s)	2562
XapI	RAATTY	7 cut(s)	474, 532, 711, 740, 1501, 2001, 2136
XbaI	TCTAGA	1 cut(s)	2054
XceI	RCATGY	2 cut(s)	773, 2581
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1120
XmiI	GTMKAC	3 cut(s)	363, 691, 1356
XmnI	GAANNNNTTC	1 cut(s)	2002
XspI	CTAG	7 cut(s)	287, 611, 755, 1535, 1875, 2055, 2111
ZraI	GACGTC	1 cut(s)	232

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.