MD12G1238100.v1.1

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0022369
Physical Location & Seq
Reverse (-)
31150430 .. 31151252
823 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1238100.v1.1.491

Sequence Viewer

Length: 771 bp
ATGTTCAACTCCTTATCTCGAATATGTGCCATCTCCCTCCCCCAAAGTAAAGCGCTGATTCCCCTAAACCCTTCTCTGTCGTCGGCCTTTTGCTTTGGCTTGAGGTTGGCGGCAACCCAGCCCTTCCCTCCTCGCCCTCTGCTCTTTTCCTCTCTCGTCCACCCACTGCTTCATCACTATCTCCCTTCTCTCCCTGTTTTTTCGCCCTTCTTTCTCTTCTCATTTTTTCCACCCTTGTTTCATACTTTCTCCCCTTCTCTCCCTTTCTTTCCTCCCGTTTTCCCCATCTATCCCTTTTCCTCTAACTCCACTACAGCTTTTCCCTCTCCAAGCCACCATGGTTGGAGATTTAGTTTATCTGTTGCTCTGAGCTTGGTCTCAGTCTTTCTTAAGTTTATCTCAGATCTGTTTTCTTTGCCTTGTTCCTCTCCAAACATCGTTCCTCACCGTGAATCTTCTATTGGTTACCCTTTTAAAGTGCTTTTTGCACTATCTGGTAGGATTATGAGAGTGCTTCCAGCACGGGTAGCTTGCCTACCCCCATTTTCCGTTTATCCATTTGGTTCGTTGGCCCTTCTCCCTGGTGGTGGTGACAGATCTGTGATGGCAGTGCGGCTGTTTTTGTGGAGGTGGCTGTTTAGGTTTTTCAATTTGGTTGCTGCTATGGGTTGTTCACTTTGGGACCTTCTCATTGTAGTTTTCGTTTATGGAGTTGGGCTTTTTCTTGGCTATTTTAGACTCTCTTTTGGAGTTGTCCTTATCTTGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5

Protein Analysis

257

Amino Acids

28.59

Weight (kDa)

10.19

Isoelectric Point (pI)

62.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022369)

Species Orthologous Gene IDs
malus_domestica MD08G1047800.v1.1 MD12G1238100.v1.1 MD16G1064600.v1.1
pyrus_communis pycom01g04510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 110, 613
AfeI AGCGCT 1 cut(s) 54
AfiI CCNNNNNNNGG 1 cut(s) 587
AflII CTTAAG 1 cut(s) 389
AgsI TTSAA 2 cut(s) 7, 649
AjnI CCWGG 1 cut(s) 580
AjuI GAANNNNNNNTTGG 2 cut(s) 444, 476
AluBI AGCT 3 cut(s) 317, 372, 530
AluI AGCT 3 cut(s) 317, 372, 530
Alw26I GTCTC 1 cut(s) 382
Aor51HI AGCGCT 1 cut(s) 54
AoxI GGCC 2 cut(s) 84, 570
ApeKI GCWGC 1 cut(s) 659
AspLEI GCGC 1 cut(s) 55
AspS9I GGNCC 2 cut(s) 571, 682
AsuHPI GGTGA 2 cut(s) 437, 602
AvaII GGWCC 1 cut(s) 682
BbvI GCAGC 1 cut(s) 646
BccI CCATC 3 cut(s) 38, 293, 598
BciT130I CCWGG 1 cut(s) 582
BcoDI GTCTC 1 cut(s) 382
BfmI CTRYAG 1 cut(s) 312
BfoI RGCGCY 1 cut(s) 56
BfrI CTTAAG 1 cut(s) 389
BglII AGATCT 2 cut(s) 403, 596
BisI GCNGC 3 cut(s) 111, 614, 660
BlsI GCNGC 3 cut(s) 112, 615, 661
Bme1390I CCNGG 1 cut(s) 582
Bme18I GGWCC 1 cut(s) 682
BmgT120I GGNCC 2 cut(s) 571, 682
BmiI GGNNCC 1 cut(s) 683
BmrFI CCNGG 1 cut(s) 582
BpuEI CTTGAG 1 cut(s) 121
BsaI GGTCTC 1 cut(s) 382
BsaJI CCNNGG 2 cut(s) 337, 580
BsaXI ACNNNNNCTCC 2 cut(s) 337, 367
Bsc4I CCNNNNNNNGG 1 cut(s) 587
BseBI CCWGG 1 cut(s) 582
BseDI CCNNGG 2 cut(s) 337, 580
BseLI CCNNNNNNNGG 1 cut(s) 587
BseMII CTCAG 3 cut(s) 359, 393, 414
BseRI GAGGAG 1 cut(s) 120
BseXI GCAGC 1 cut(s) 646
BseYI CCCAGC 1 cut(s) 117
BshFI GGCC 2 cut(s) 86, 572
BslFI GGGAC 1 cut(s) 695
BslI CCNNNNNNNGG 1 cut(s) 587
BsmAI GTCTC 1 cut(s) 382
BsmFI GGGAC 1 cut(s) 695
BsnI GGCC 2 cut(s) 86, 572
Bso31I GGTCTC 1 cut(s) 382
Bsp143I GATC 2 cut(s) 403, 596
Bsp19I CCATGG 1 cut(s) 337
BspACI CCGC 2 cut(s) 110, 613
BspANI GGCC 2 cut(s) 86, 572
BspCNI CTCAG 3 cut(s) 360, 392, 413
BspLI GGNNCC 1 cut(s) 683
BspTI CTTAAG 1 cut(s) 389
BspTNI GGTCTC 1 cut(s) 382
BssECI CCNNGG 2 cut(s) 337, 580
BssMI GATC 2 cut(s) 403, 596
BssT1I CCWWGG 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 582
Bst4CI ACNGT 1 cut(s) 449
Bst6I CTCTTC 1 cut(s) 221
BstAFI CTTAAG 1 cut(s) 389
BstC8I GCNNGC 1 cut(s) 532
BstDEI CTNAG 3 cut(s) 368, 379, 400
BstDSI CCRYGG 1 cut(s) 337
BstEII GGTNACC 1 cut(s) 464
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 2 cut(s) 406, 599
BstMAI GTCTC 1 cut(s) 382
BstMBI GATC 2 cut(s) 403, 596
BstMWI GCNNNNNNNGC 1 cut(s) 527
BstNI CCWGG 1 cut(s) 582
BstPI GGTNACC 1 cut(s) 464
BstSCI CCNGG 1 cut(s) 580
BstSFI CTRYAG 1 cut(s) 312
BstV1I GCAGC 1 cut(s) 646
BstX2I RGATCY 2 cut(s) 403, 596
BstYI RGATCY 2 cut(s) 403, 596
BsuRI GGCC 2 cut(s) 86, 572
BtgI CCRYGG 1 cut(s) 337
BtsI GCAGTG 2 cut(s) 164, 615
BtsIMutI CAGTG 2 cut(s) 164, 615
Cac8I GCNNGC 1 cut(s) 532
CfoI GCGC 1 cut(s) 55
Cfr13I GGNCC 2 cut(s) 571, 682
CspCI CAANNNNNGTGG 2 cut(s) 323, 358
CviAII CATG 1 cut(s) 338
DdeI CTNAG 3 cut(s) 368, 379, 400
DpnI GATC 2 cut(s) 405, 598
DpnII GATC 2 cut(s) 403, 596
DraI TTTAAA 1 cut(s) 475
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
Eco130I CCWWGG 1 cut(s) 337
Eco31I GGTCTC 1 cut(s) 382
Eco47I GGWCC 1 cut(s) 682
Eco47III AGCGCT 1 cut(s) 54
Eco91I GGTNACC 1 cut(s) 464
EcoO109I RGGNCCY 1 cut(s) 682
EcoO65I GGTNACC 1 cut(s) 464
EcoRII CCWGG 1 cut(s) 580
EcoT14I CCWWGG 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 337
FaeI CATG 1 cut(s) 341
FaiI YATR 6 cut(s) 25, 243, 339, 506, 665, 708
FaqI GGGAC 1 cut(s) 695
FatI CATG 1 cut(s) 337
Fnu4HI GCNGC 3 cut(s) 111, 614, 660
Fsp4HI GCNGC 3 cut(s) 111, 614, 660
GlaI GCGC 1 cut(s) 54
GluI GCNGC 3 cut(s) 111, 614, 660
GsaI CCCAGC 1 cut(s) 121
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 2 cut(s) 86, 572
HhaI GCGC 1 cut(s) 55
Hin1II CATG 1 cut(s) 341
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HinfI GANTC 3 cut(s) 58, 452, 738
HphI GGTGA 2 cut(s) 437, 602
Hpy166II GTNNAC 2 cut(s) 160, 674
Hpy188I TCNGA 2 cut(s) 369, 403
Hpy188III TCNNGA 1 cut(s) 18
Hpy8I GTNNAC 2 cut(s) 160, 674
Hpy99I CGWCG 1 cut(s) 85
HpyAV CCTTC 7 cut(s) 81, 133, 195, 217, 264, 584, 695
HpyCH4III ACNGT 1 cut(s) 449
HpyCH4V TGCA 1 cut(s) 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 527
HpyF3I CTNAG 3 cut(s) 368, 379, 400
Hsp92II CATG 1 cut(s) 341
HspAI GCGC 1 cut(s) 53
Kzo9I GATC 2 cut(s) 403, 596
LpnPI CCDG 6 cut(s) 131, 207, 480, 531, 567, 594
Lsp1109I GCAGC 1 cut(s) 646
MaeIII GTNAC 2 cut(s) 464, 590
MalI GATC 2 cut(s) 405, 598
MboI GATC 2 cut(s) 403, 596
MboII GAAGA 2 cut(s) 208, 447
MflI RGATCY 2 cut(s) 403, 596
MluCI AATT 1 cut(s) 649
MlyI GAGTC 1 cut(s) 732
MmeI TCCRAC 1 cut(s) 323
MseI TTAA 3 cut(s) 390, 474, 769
MspCI CTTAAG 1 cut(s) 389
MspR9I CCNGG 1 cut(s) 582
MvaI CCWGG 1 cut(s) 582
MwoI GCNNNNNNNGC 1 cut(s) 527
NcoI CCATGG 1 cut(s) 337
NdeII GATC 2 cut(s) 403, 596
NlaIII CATG 1 cut(s) 341
NlaIV GGNNCC 1 cut(s) 683
NmuCI GTSAC 1 cut(s) 590
PfeI GAWTC 2 cut(s) 58, 452
PkrI GCNGC 3 cut(s) 112, 615, 661
PleI GAGTC 1 cut(s) 732
PpsI GAGTC 1 cut(s) 732
PpuMI RGGWCCY 1 cut(s) 682
Psp5II RGGWCCY 1 cut(s) 682
Psp6I CCWGG 1 cut(s) 580
PspEI GGTNACC 1 cut(s) 464
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 1 cut(s) 580
PspN4I GGNNCC 1 cut(s) 683
PspPI GGNCC 2 cut(s) 571, 682
PspPPI RGGWCCY 1 cut(s) 682
PsuI RGATCY 2 cut(s) 403, 596
SaqAI TTAA 3 cut(s) 390, 474, 769
SatI GCNGC 3 cut(s) 111, 614, 660
Sau3AI GATC 2 cut(s) 403, 596
Sau96I GGNCC 2 cut(s) 571, 682
SchI GAGTC 1 cut(s) 732
ScrFI CCNGG 1 cut(s) 582
SetI ASST 7 cut(s) 107, 319, 374, 532, 632, 644, 687
SfcI CTRYAG 1 cut(s) 312
SinI GGWCC 1 cut(s) 682
SmlI CTYRAG 2 cut(s) 100, 389
SmoI CTYRAG 2 cut(s) 100, 389
Sse9I AATT 1 cut(s) 649
SsiI CCGC 2 cut(s) 110, 613
StyD4I CCNGG 1 cut(s) 580
StyI CCWWGG 1 cut(s) 337
TaaI ACNGT 1 cut(s) 449
TaqI TCGA 1 cut(s) 19
TasI AATT 1 cut(s) 649
TauI GCSGC 2 cut(s) 113, 616
TfiI GAWTC 2 cut(s) 58, 452
Tru1I TTAA 3 cut(s) 390, 474, 769
Tru9I TTAA 3 cut(s) 390, 474, 769
TscAI CASTG 2 cut(s) 171, 615
TseFI GTSAC 1 cut(s) 590
TseI GCWGC 1 cut(s) 659
Tsp45I GTSAC 1 cut(s) 590
TspDTI ATGAA 2 cut(s) 161, 230
TspGWI ACGGA 1 cut(s) 538
TspRI CASTG 2 cut(s) 171, 615
Vha464I CTTAAG 1 cut(s) 389
VpaK11BI GGWCC 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.