MD16G1064600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0022369
Physical Location & Seq
Forward (+)
4541100 .. 4541679
580 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1064600.v1.1.491

Sequence Viewer

Length: 285 bp
ATGGTTGGAGATTTAGTTGCTCTGAGTTTGGTCTCAGTATTCCCTGAGATTATGGGAGCGCTTCCAACACGAGGAGCTTGCCTACCCCCATTTTCTGTTCATCATTTTAGTTCTTTGGCCCTTTTTCCGGGTGGTGGTGACAGATCCGCGGTGGCGATGCGGTTGTGTTGGTGGAGGTGGCTGTCTATGGTTTTTGATTTGGTTGCTGTTATAGGCTGCTCACTTTGGGCCCTTCTCATTGTAATTTTCGTTTATGGTGGGTGTGGCCGGCCCCTTTTGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

95

Amino Acids

10.19

Weight (kDa)

8.52

Isoelectric Point (pI)

50.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022369)

Species Orthologous Gene IDs
malus_domestica MD08G1047800.v1.1 MD12G1238100.v1.1 MD16G1064600.v1.1
pyrus_communis pycom01g04510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 149
AciI CCGC 3 cut(s) 147, 149, 160
AclWI GGATC 1 cut(s) 138
AcoI YGGCCR 1 cut(s) 265
AfeI AGCGCT 1 cut(s) 60
AfiI CCNNNNNNNGG 3 cut(s) 71, 127, 134
AluBI AGCT 1 cut(s) 77
AluI AGCT 1 cut(s) 77
Alw26I GTCTC 1 cut(s) 37
AlwI GGATC 1 cut(s) 138
Aor51HI AGCGCT 1 cut(s) 60
AoxI GGCC 4 cut(s) 117, 228, 265, 269
ApaI GGGCCC 1 cut(s) 232
ApeKI GCWGC 1 cut(s) 216
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 4 cut(s) 118, 228, 229, 270
AsuC2I CCSGG 1 cut(s) 129
AsuHPI GGTGA 1 cut(s) 149
BaeGI GKGCMC 1 cut(s) 232
BanII GRGCYC 1 cut(s) 232
BauI CACGAG 1 cut(s) 69
BbvI GCAGC 1 cut(s) 203
BcgI CGANNNNNNTGC 2 cut(s) 60, 94
BcnI CCSGG 1 cut(s) 129
BcoDI GTCTC 1 cut(s) 37
BfoI RGCGCY 1 cut(s) 62
BisI GCNGC 1 cut(s) 217
BlsI GCNGC 1 cut(s) 218
Bme1390I CCNGG 1 cut(s) 129
BmgT120I GGNCC 4 cut(s) 118, 228, 229, 270
BmiI GGNNCC 2 cut(s) 230, 272
BmrFI CCNGG 1 cut(s) 129
BmsI GCATC 1 cut(s) 147
BpuMI CCSGG 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 1 cut(s) 147
BsaXI ACNNNNNCTCC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 3 cut(s) 71, 127, 134
Bse118I RCCGGY 1 cut(s) 267
BseDI CCNNGG 1 cut(s) 147
BseLI CCNNNNNNNGG 3 cut(s) 71, 127, 134
BseMII CTCAG 3 cut(s) 14, 36, 48
BseRI GAGGAG 1 cut(s) 87
BseSI GKGCMC 1 cut(s) 232
BseXI GCAGC 1 cut(s) 203
Bsh1236I CGCG 1 cut(s) 149
BshFI GGCC 4 cut(s) 119, 230, 267, 271
BsiSI CCGG 2 cut(s) 128, 268
BslI CCNNNNNNNGG 3 cut(s) 71, 127, 134
BsmAI GTCTC 1 cut(s) 37
BsnI GGCC 4 cut(s) 119, 230, 267, 271
Bso31I GGTCTC 1 cut(s) 37
Bsp120I GGGCCC 1 cut(s) 228
Bsp1286I GDGCHC 1 cut(s) 232
Bsp143I GATC 1 cut(s) 143
BspACI CCGC 3 cut(s) 147, 149, 160
BspANI GGCC 4 cut(s) 119, 230, 267, 271
BspCNI CTCAG 3 cut(s) 15, 37, 47
BspFNI CGCG 1 cut(s) 149
BspLI GGNNCC 2 cut(s) 230, 272
BspPI GGATC 1 cut(s) 138
BspTNI GGTCTC 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 267
BssAI RCCGGY 1 cut(s) 267
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 1 cut(s) 143
BssSI CACGAG 1 cut(s) 69
Bst2BI CACGAG 1 cut(s) 69
BstC8I GCNNGC 2 cut(s) 79, 269
BstDEI CTNAG 3 cut(s) 23, 34, 45
BstDSI CCRYGG 1 cut(s) 147
BstFNI CGCG 1 cut(s) 149
BstH2I RGCGCY 1 cut(s) 62
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 1 cut(s) 146
BstMAI GTCTC 1 cut(s) 37
BstMBI GATC 1 cut(s) 143
BstSCI CCNGG 1 cut(s) 127
BstSLI GKGCMC 1 cut(s) 232
BstUI CGCG 1 cut(s) 149
BstV1I GCAGC 1 cut(s) 203
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BsuRI GGCC 4 cut(s) 119, 230, 267, 271
BtgI CCRYGG 1 cut(s) 147
BtgZI GCGATG 1 cut(s) 170
Cac8I GCNNGC 2 cut(s) 79, 269
CfoI GCGC 1 cut(s) 61
Cfr10I RCCGGY 1 cut(s) 267
Cfr13I GGNCC 4 cut(s) 118, 228, 229, 270
Cfr42I CCGCGG 1 cut(s) 150
CviJI RGCY 7 cut(s) 77, 119, 181, 216, 230, 267, 271
CviKI_1 RGCY 7 cut(s) 77, 119, 181, 216, 230, 267, 271
DdeI CTNAG 3 cut(s) 23, 34, 45
DpnI GATC 1 cut(s) 145
DpnII GATC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 232
Eco31I GGTCTC 1 cut(s) 37
Eco47III AGCGCT 1 cut(s) 60
EcoO109I RGGNCCY 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 232
FaiI YATR 4 cut(s) 53, 188, 212, 255
Fnu4HI GCNGC 1 cut(s) 217
FriOI GRGCYC 1 cut(s) 232
FseI GGCCGGCC 1 cut(s) 271
Fsp4HI GCNGC 1 cut(s) 217
GlaI GCGC 1 cut(s) 60
GluI GCNGC 1 cut(s) 217
HaeII RGCGCY 1 cut(s) 62
HaeIII GGCC 4 cut(s) 119, 230, 267, 271
HapII CCGG 2 cut(s) 128, 268
HhaI GCGC 1 cut(s) 61
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HpaII CCGG 2 cut(s) 128, 268
HphI GGTGA 1 cut(s) 149
Hpy188I TCNGA 1 cut(s) 24
HpyAV CCTTC 1 cut(s) 242
HpyF3I CTNAG 3 cut(s) 23, 34, 45
HspAI GCGC 1 cut(s) 59
KroI GCCGGC 1 cut(s) 267
KroNI GCCGGC 1 cut(s) 269
KspI CCGCGG 1 cut(s) 150
Kzo9I GATC 1 cut(s) 143
LmnI GCTCC 2 cut(s) 56, 74
LpnPI CCDG 3 cut(s) 57, 141, 281
Lsp1109I GCAGC 1 cut(s) 203
LweI GCATC 1 cut(s) 147
MaeIII GTNAC 1 cut(s) 137
MalI GATC 1 cut(s) 145
MboI GATC 1 cut(s) 143
MflI RGATCY 1 cut(s) 143
MhlI GDGCHC 1 cut(s) 232
MluCI AATT 1 cut(s) 243
MmeI TCCRAC 1 cut(s) 89
MnlI CCTC 2 cut(s) 65, 168
MroNI GCCGGC 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 2 cut(s) 128, 268
MspR9I CCNGG 1 cut(s) 129
MvnI CGCG 1 cut(s) 149
NaeI GCCGGC 1 cut(s) 269
NciI CCSGG 1 cut(s) 129
NdeII GATC 1 cut(s) 143
NgoMIV GCCGGC 1 cut(s) 267
NlaIV GGNNCC 2 cut(s) 230, 272
NmuCI GTSAC 1 cut(s) 137
PdiI GCCGGC 1 cut(s) 269
PkrI GCNGC 1 cut(s) 218
PspN4I GGNNCC 2 cut(s) 230, 272
PspOMI GGGCCC 1 cut(s) 228
PspPI GGNCC 4 cut(s) 118, 228, 229, 270
PsuI RGATCY 1 cut(s) 143
RigI GGCCGGCC 1 cut(s) 271
SacII CCGCGG 1 cut(s) 150
SatI GCNGC 1 cut(s) 217
Sau3AI GATC 1 cut(s) 143
Sau96I GGNCC 4 cut(s) 118, 228, 229, 270
ScrFI CCNGG 1 cut(s) 129
SduI GDGCHC 1 cut(s) 232
SetI ASST 2 cut(s) 79, 179
SfaNI GCATC 1 cut(s) 147
Sfr303I CCGCGG 1 cut(s) 150
SgeI CNNG 8 cut(s) 56, 81, 83, 90, 140, 141, 160, 280
SgrBI CCGCGG 1 cut(s) 150
Sse9I AATT 1 cut(s) 243
SsiI CCGC 3 cut(s) 147, 149, 160
StyD4I CCNGG 1 cut(s) 127
TasI AATT 1 cut(s) 243
TseFI GTSAC 1 cut(s) 137
TseI GCWGC 1 cut(s) 216
Tsp45I GTSAC 1 cut(s) 137
TspDTI ATGAA 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.