Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0021191

Physical Location & Seq

Genomic Coordinates Forward (+)

32173559 .. 32176718

Gene Structure 3160 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1252700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1326 bp

ATGGCTGTTCCAGTTGAAGAAGCCATTGCCGCTCTATCCACCTTCTCACTCGAGGATGAGCAAGCGGAGGTTCAAGGACCGGGAATTTGGGTTTCGACTGATACCGGCGCAACGGATAGTCCCATCGAGTATAGCGATGTTTCCGCTTATCGCCTATCGTTGACAGAAGACACAAAAGCTCTCAATCAGCTGAATGCTCTTATTCAAGAGGGAAAGGAGATGGGATCGGTGCTTTATACATATCGCAGTTGTGTCAAAGCACTTCCTCAGCTTCCTGATAATATGAAACAAAGTCAAGCTGACTTGTATCTAGAAACATATCAAGTTTTGGACTTGGAAATGAGCCGTTTGCGTGAAATACAGCGATGGCAAGCATCAGCTGCCGCAAAGCTAGCTGCAGATATGCAACGGTTTTCTAGGCCTGAGCGACGCATTAATGGCCCCACAGTAACTCATCTCTGGTCCATGTTGAAGTTACTTGACACTCTGGTGCAACTTGATCATCTCAAAAATGCCAAAGCAAGCATACCGAATGATTTCTCTTGGTATAAAAGGACATTCACACAAGTAAGTGTACAGTGGCATGATACTGATACGATGAGAGAGGAGCTAGATGACTTACAGATTTTCCTTAGCACAAGGTGGGCTATCTTATTGAATTTGCATGTGGAGATGTTCCGCGTGAACAATGTAGAAGACATCCTTCAAGTTCTCATAGTCTTTGCTGTTGAGTCATTAGAGCTGGATTTTGCACTTCTGTTCCCAGAGCGGCATATTCTTCTACGTGTTTTGCCAATTCTTGTTGTTTTAGCAACATCATCGGAAAAAGATAGCGAGTCTTTGTACAAGAGAGTGAAAATCAACAGACTGATCAATATATTTAAGAATGATCCAGTAATTCCTGCATTTCCAGATCTTCATCTTTCTCCTGTTGCAATCATGAAAGAATTGTCAATATACTTCCAGAAGTTTTCCACCCAAACTCGTCTTCTTAGTCTTCCCTCACCTCATGAGCTTCCATCCCGCGAGGCACAAGAATATCCTTTTGATTTGTTTTCATTATTTATTAAAAGTAAAATTTTATTGTTGTTGAAGGCAACAGACAATCCAGATATTGATTGGTGCAAAGAAGTGAAGGGAAACATATATGATATGGTTGTTGAAGGTTTTCAGCTGTTGAGTAGATGGACTGCACGCATTTGGGAACAATGTGCATGGAAGTTTTCTCGTCCCTGCAAGGATATAGTTCCTTCTGAAACACAAGAGGCTTTAGCATCATTTTCTGACTATGAAAAGGTATTGTCTCCCATATCTGAAATGTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008150 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010638 GO:0016043 GO:0030029 GO:0030036 GO:0030154 GO:0030832 GO:0030833 GO:0030838 GO:0031209 GO:0031334 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032989 GO:0032991 GO:0033043 GO:0043254 GO:0044087 GO:0044089 GO:0044424 GO:0044444 GO:0044464 GO:0045010 GO:0048468 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051493 GO:0051495 GO:0065007 GO:0065008 GO:0071840 GO:0090066 GO:0090558 GO:0090626 GO:0097435 GO:0110053 GO:1902903 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko03013 ko04810 map03013 map04810

Pfam Domains

Protein Families

DUF1394 FragX_IP

Protein Analysis

442

Amino Acids

50.67

Weight (kDa)

5.02

Isoelectric Point (pI)

47.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FragX_IP	PF05994	370 - 433	5.7e-14	Cytoplasmic Fragile-X interacting family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0021191)

Species	Orthologous Gene IDs
malus_domestica	MD12G1252700.v1.1
rosa_chinensis	RchiOBHm_Chr3g0448911
rosa_samantha	Rh3BG015300 Rh3CG014200 Rh3DG015800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	2 cut(s)	32, 769
AccII	CGCG	2 cut(s)	681, 1026
AciI	CCGC	7 cut(s)	30, 65, 144, 384, 679, 769, 1024
AclWI	GGATC	2 cut(s)	232, 884
AcsI	RAATTY	3 cut(s)	84, 658, 1077
AdeI	CACNNNGTG	1 cut(s)	642
AfaI	GTAC	2 cut(s)	576, 845
AflIII	ACRYGT	1 cut(s)	784
AgsI	TTSAA	8 cut(s)	17, 74, 206, 472, 658, 707, 1093, 1163
AjuI	GAANNNNNNNTTGG	2 cut(s)	972, 1004
AleI	CACNNNNGTG	1 cut(s)	488
Alw26I	GTCTC	1 cut(s)	1308
AlwI	GGATC	2 cut(s)	232, 884
Ama87I	CYCGRG	1 cut(s)	50
AoxI	GGCC	2 cut(s)	419, 439
ApeKI	GCWGC	2 cut(s)	380, 395
ApoI	RAATTY	3 cut(s)	84, 658, 1077
ArsI	GACNNNNNNTTYG	2 cut(s)	69, 101
AseI	ATTAAT	1 cut(s)	435
Asp700I	GAANNNNTTC	2 cut(s)	536, 1167
AspLEI	GCGC	1 cut(s)	110
AspS9I	GGNCC	3 cut(s)	77, 440, 462
AsuC2I	CCSGG	1 cut(s)	81
AsuHPI	GGTGA	1 cut(s)	996
AsuNHI	GCTAGC	1 cut(s)	391
AvaI	CYCGRG	1 cut(s)	50
AvaII	GGWCC	2 cut(s)	77, 462
BbsI	GAAGAC	4 cut(s)	174, 702, 980, 989
BbvCI	CCTCAGC	1 cut(s)	267
BbvI	GCAGC	2 cut(s)	367, 382
BccI	CCATC	5 cut(s)	131, 214, 360, 1027, 1179
BceAI	ACGGC	1 cut(s)	330
BcgI	CGANNNNNNTGC	2 cut(s)	801, 835
BclI	TGATCA	2 cut(s)	499, 870
BcnI	CCSGG	1 cut(s)	81
BcoDI	GTCTC	1 cut(s)	1308
BfaI	CTAG	4 cut(s)	311, 392, 417, 611
BfmI	CTRYAG	1 cut(s)	396
BglII	AGATCT	1 cut(s)	913
BisI	GCNGC	5 cut(s)	30, 381, 384, 396, 770
BlsI	GCNGC	5 cut(s)	31, 382, 385, 397, 771
Bme1390I	CCNGG	1 cut(s)	81
Bme18I	GGWCC	2 cut(s)	77, 462
BmeT110I	CYCGRG	1 cut(s)	50
BmgT120I	GGNCC	3 cut(s)	77, 440, 462
BmiI	GGNNCC	1 cut(s)	442
BmrFI	CCNGG	1 cut(s)	81
BmsI	GCATC	2 cut(s)	383, 1283
BmtI	GCTAGC	1 cut(s)	395
BpiI	GAAGAC	4 cut(s)	174, 702, 980, 989
Bpu10I	CCTNAGC	3 cut(s)	267, 423, 632
BpuMI	CCSGG	1 cut(s)	81
BsaAI	YACGTR	1 cut(s)	785
BsaBI	GATNNNNATC	1 cut(s)	918
Bse118I	RCCGGY	1 cut(s)	104
Bse1I	ACTGG	2 cut(s)	11, 893
Bse3DI	GCAATG	1 cut(s)	24
Bse8I	GATNNNNATC	1 cut(s)	918
BseGI	GGATG	3 cut(s)	61, 699, 1019
BseJI	GATNNNNATC	1 cut(s)	918
BseMI	GCAATG	1 cut(s)	24
BseMII	CTCAG	2 cut(s)	281, 414
BseNI	ACTGG	2 cut(s)	11, 893
BseRI	GAGGAG	1 cut(s)	620
BseXI	GCAGC	2 cut(s)	367, 382
BsgI	GTGCAG	1 cut(s)	1176
Bsh1236I	CGCG	2 cut(s)	681, 1026
BshFI	GGCC	2 cut(s)	421, 441
BsiHKCI	CYCGRG	1 cut(s)	50
BsiSI	CCGG	2 cut(s)	80, 105
BslFI	GGGAC	2 cut(s)	105, 1215
BsmAI	GTCTC	1 cut(s)	1308
BsmFI	GGGAC	2 cut(s)	105, 1215
BsmI	GAATGC	1 cut(s)	199
BsnI	GGCC	2 cut(s)	421, 441
BsoBI	CYCGRG	1 cut(s)	50
Bsp1407I	TGTACA	2 cut(s)	574, 843
Bsp143I	GATC	5 cut(s)	224, 499, 870, 889, 913
BspACI	CCGC	7 cut(s)	30, 65, 144, 384, 679, 769, 1024
BspANI	GGCC	2 cut(s)	421, 441
BspCNI	CTCAG	2 cut(s)	280, 415
BspFNI	CGCG	2 cut(s)	681, 1026
BspHI	TCATGA	2 cut(s)	939, 1009
BspLI	GGNNCC	1 cut(s)	442
BspMAI	CTGCAG	1 cut(s)	400
BspOI	GCTAGC	1 cut(s)	395
BspPI	GGATC	2 cut(s)	232, 884
BsrBI	CCGCTC	2 cut(s)	32, 769
BsrDI	GCAATG	1 cut(s)	24
BsrFI	RCCGGY	1 cut(s)	104
BsrGI	TGTACA	2 cut(s)	574, 843
BsrI	ACTGG	2 cut(s)	11, 893
BssAI	RCCGGY	1 cut(s)	104
BssMI	GATC	5 cut(s)	224, 499, 870, 889, 913
Bst4CI	ACNGT	3 cut(s)	411, 448, 579
BstAPI	GCANNNNNTGC	1 cut(s)	380
BstAUI	TGTACA	2 cut(s)	574, 843
BstBAI	YACGTR	1 cut(s)	785
BstC8I	GCNNGC	5 cut(s)	63, 372, 393, 523, 1195
BstDEI	CTNAG	4 cut(s)	267, 423, 632, 992
BstF5I	GGATG	3 cut(s)	61, 699, 1019
BstFNI	CGCG	2 cut(s)	681, 1026
BstHHI	GCGC	1 cut(s)	110
BstKTI	GATC	5 cut(s)	227, 502, 873, 892, 916
BstMAI	GTCTC	1 cut(s)	1308
BstMBI	GATC	5 cut(s)	224, 499, 870, 889, 913
BstMWI	GCNNNNNNNGC	4 cut(s)	29, 380, 392, 438
BstNSI	RCATGY	1 cut(s)	668
BstSCI	CCNGG	1 cut(s)	79
BstSFI	CTRYAG	1 cut(s)	396
BstUI	CGCG	2 cut(s)	681, 1026
BstV1I	GCAGC	2 cut(s)	367, 382
BstV2I	GAAGAC	4 cut(s)	174, 702, 980, 989
BstX2I	RGATCY	1 cut(s)	913
BstYI	RGATCY	1 cut(s)	913
BsuRI	GGCC	2 cut(s)	421, 441
BtgZI	GCGATG	2 cut(s)	150, 379
BtsCI	GGATG	3 cut(s)	61, 699, 1019
BtsIMutI	CAGTG	1 cut(s)	584
Cac8I	GCNNGC	5 cut(s)	63, 372, 393, 523, 1195
CciI	TCATGA	2 cut(s)	939, 1009
CfoI	GCGC	1 cut(s)	110
Cfr10I	RCCGGY	1 cut(s)	104
Cfr13I	GGNCC	3 cut(s)	77, 440, 462
CseI	GACGC	1 cut(s)	438
Csp6I	GTAC	2 cut(s)	575, 844
CviAII	CATG	6 cut(s)	466, 584, 665, 940, 1010, 1215
CviQI	GTAC	2 cut(s)	575, 844
DdeI	CTNAG	4 cut(s)	267, 423, 632, 992
DpnI	GATC	5 cut(s)	226, 501, 872, 891, 915
DpnII	GATC	5 cut(s)	224, 499, 870, 889, 913
DraIII	CACNNNGTG	1 cut(s)	642
Eco147I	AGGCCT	1 cut(s)	421
Eco47I	GGWCC	2 cut(s)	77, 462
Eco88I	CYCGRG	1 cut(s)	50
FaeI	CATG	6 cut(s)	469, 587, 668, 943, 1013, 1218
FalI	AAGNNNNNCTT	2 cut(s)	687, 719
FaqI	GGGAC	2 cut(s)	105, 1215
FatI	CATG	6 cut(s)	465, 583, 664, 939, 1009, 1214
FauI	CCCGC	1 cut(s)	1031
FbaI	TGATCA	2 cut(s)	499, 870
Fnu4HI	GCNGC	5 cut(s)	30, 381, 384, 396, 770
FokI	GGATG	3 cut(s)	68, 686, 1006
Fsp4HI	GCNGC	5 cut(s)	30, 381, 384, 396, 770
FspBI	CTAG	4 cut(s)	311, 392, 417, 611
GlaI	GCGC	1 cut(s)	109
GluI	GCNGC	5 cut(s)	30, 381, 384, 396, 770
HaeIII	GGCC	2 cut(s)	421, 441
HapII	CCGG	2 cut(s)	80, 105
HgaI	GACGC	1 cut(s)	438
HhaI	GCGC	1 cut(s)	110
Hin1II	CATG	6 cut(s)	469, 587, 668, 943, 1013, 1218
Hin6I	GCGC	1 cut(s)	108
HinP1I	GCGC	1 cut(s)	108
HincII	GTYRAC	1 cut(s)	162
HindII	GTYRAC	1 cut(s)	162
HinfI	GANTC	2 cut(s)	731, 836
HpaII	CCGG	2 cut(s)	80, 105
HphI	GGTGA	1 cut(s)	996
Hpy166II	GTNNAC	3 cut(s)	162, 575, 685
Hpy188I	TCNGA	4 cut(s)	823, 1255, 1285, 1315
Hpy188III	TCNNGA	8 cut(s)	206, 275, 311, 911, 940, 964, 1010, 1109
Hpy8I	GTNNAC	3 cut(s)	162, 575, 685
Hpy99I	CGWCG	1 cut(s)	432
HpyAV	CCTTC	6 cut(s)	52, 713, 1087, 1129, 1157, 1260
HpyCH4III	ACNGT	3 cut(s)	411, 448, 579
HpyCH4IV	ACGT	1 cut(s)	784
HpyF10VI	GCNNNNNNNGC	4 cut(s)	29, 380, 392, 438
HpyF3I	CTNAG	4 cut(s)	267, 423, 632, 992
HpySE526I	ACGT	1 cut(s)	784
Hsp92II	CATG	6 cut(s)	469, 587, 668, 943, 1013, 1218
HspAI	GCGC	1 cut(s)	108
Ksp22I	TGATCA	2 cut(s)	499, 870
Kzo9I	GATC	5 cut(s)	224, 499, 870, 889, 913
LmnI	GCTCC	1 cut(s)	607
Lsp1109I	GCAGC	2 cut(s)	367, 382
LweI	GCATC	2 cut(s)	383, 1283
MaeI	CTAG	4 cut(s)	311, 392, 417, 611
MaeII	ACGT	1 cut(s)	784
MaeIII	GTNAC	2 cut(s)	448, 474
MalI	GATC	5 cut(s)	226, 501, 872, 891, 915
MbiI	CCGCTC	2 cut(s)	32, 769
MboI	GATC	5 cut(s)	224, 499, 870, 889, 913
MboII	GAAGA	7 cut(s)	29, 179, 707, 770, 908, 980, 989
MflI	RGATCY	1 cut(s)	913
MluCI	AATT	6 cut(s)	84, 658, 795, 897, 947, 1077
MlyI	GAGTC	2 cut(s)	740, 845
MnlI	CCTC	9 cut(s)	46, 61, 202, 276, 598, 1012, 1017, 1021, 1258
MroXI	GAANNNNTTC	2 cut(s)	536, 1167
MseI	TTAA	3 cut(s)	435, 882, 1068
MslI	CAYNNNNRTG	1 cut(s)	488
MspA1I	CMGCKG	3 cut(s)	190, 380, 1174
MspI	CCGG	2 cut(s)	80, 105
MspR9I	CCNGG	1 cut(s)	81
Mva1269I	GAATGC	1 cut(s)	199
MvnI	CGCG	2 cut(s)	681, 1026
MwoI	GCNNNNNNNGC	4 cut(s)	29, 380, 392, 438
NciI	CCSGG	1 cut(s)	81
NdeII	GATC	5 cut(s)	224, 499, 870, 889, 913
NheI	GCTAGC	1 cut(s)	391
NlaIII	CATG	6 cut(s)	469, 587, 668, 943, 1013, 1218
NlaIV	GGNNCC	1 cut(s)	442
NspI	RCATGY	1 cut(s)	668
OliI	CACNNNNGTG	1 cut(s)	488
PaeR7I	CTCGAG	1 cut(s)	50
PagI	TCATGA	2 cut(s)	939, 1009
PceI	AGGCCT	1 cut(s)	421
PcsI	WCGNNNNNNNCGW	1 cut(s)	132
PctI	GAATGC	1 cut(s)	199
PdmI	GAANNNNTTC	2 cut(s)	536, 1167
PkrI	GCNGC	5 cut(s)	31, 382, 385, 397, 771
PleI	GAGTC	2 cut(s)	739, 844
PpsI	GAGTC	2 cut(s)	739, 844
Ppu21I	YACGTR	1 cut(s)	785
PshBI	ATTAAT	1 cut(s)	435
PspN4I	GGNNCC	1 cut(s)	442
PspPI	GGNCC	3 cut(s)	77, 440, 462
PspXI	VCTCGAGB	1 cut(s)	50
PstI	CTGCAG	1 cut(s)	400
PsuI	RGATCY	1 cut(s)	913
PvuII	CAGCTG	3 cut(s)	190, 380, 1174
RsaI	GTAC	2 cut(s)	576, 845
RsaNI	GTAC	2 cut(s)	575, 844
RseI	CAYNNNNRTG	1 cut(s)	488
SaqAI	TTAA	3 cut(s)	435, 882, 1068
SatI	GCNGC	5 cut(s)	30, 381, 384, 396, 770
Sau3AI	GATC	5 cut(s)	224, 499, 870, 889, 913
Sau96I	GGNCC	3 cut(s)	77, 440, 462
SchI	GAGTC	2 cut(s)	740, 845
ScrFI	CCNGG	1 cut(s)	81
SfaNI	GCATC	2 cut(s)	383, 1283
SfcI	CTRYAG	1 cut(s)	396
Sfr274I	CTCGAG	1 cut(s)	50
SinI	GGWCC	2 cut(s)	77, 462
SlaI	CTCGAG	1 cut(s)	50
SmiMI	CAYNNNNRTG	1 cut(s)	488
SmlI	CTYRAG	1 cut(s)	50
SmoI	CTYRAG	1 cut(s)	50
Sse9I	AATT	6 cut(s)	84, 658, 795, 897, 947, 1077
SseBI	AGGCCT	1 cut(s)	421
SsiI	CCGC	7 cut(s)	30, 65, 144, 384, 679, 769, 1024
SspMI	CTAG	4 cut(s)	311, 392, 417, 611
StuI	AGGCCT	1 cut(s)	421
StyD4I	CCNGG	1 cut(s)	79
TaaI	ACNGT	3 cut(s)	411, 448, 579
TaiI	ACGT	1 cut(s)	787
TaqI	TCGA	3 cut(s)	51, 95, 126
TasI	AATT	6 cut(s)	84, 658, 795, 897, 947, 1077
TatI	WGTACW	2 cut(s)	574, 843
TauI	GCSGC	3 cut(s)	32, 386, 772
Tru1I	TTAA	3 cut(s)	435, 882, 1068
Tru9I	TTAA	3 cut(s)	435, 882, 1068
TscAI	CASTG	1 cut(s)	584
TseI	GCWGC	2 cut(s)	380, 395
TspDTI	ATGAA	5 cut(s)	299, 908, 956, 1047, 1305
TspGWI	ACGGA	1 cut(s)	128
TspRI	CASTG	1 cut(s)	584
VpaK11BI	GGWCC	2 cut(s)	77, 462
VspI	ATTAAT	1 cut(s)	435
XapI	RAATTY	3 cut(s)	84, 658, 1077
XbaI	TCTAGA	1 cut(s)	310
XceI	RCATGY	1 cut(s)	668
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1116
XhoI	CTCGAG	1 cut(s)	50
XmnI	GAANNNNTTC	2 cut(s)	536, 1167
XspI	CTAG	4 cut(s)	311, 392, 417, 611

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.