MD13G1041800.v1.1

RuBisCO catalyzes two reactions the carboxylation of D- ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0024250
Physical Location & Seq
Reverse (-)
2862219 .. 2862689
471 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1041800.v1.1.491

Sequence Viewer

Length: 168 bp
ATGTGGAAGCTTCCCCTCTTCGGATGCACTGATTCTTCCCAAGTTCTCAAGGAGCTCGAGGAGGCCAAGACCACCTACCCCGAATCCTTCATCCGGATCATCGGATTCGACAACATCCGCCAAACACAGTGCGTCAGTTTCATCGCTTACAAGCCCCCAGGCTTCTAA

Protein Analysis

56

Amino Acids

6.35

Weight (kDa)

6.09

Isoelectric Point (pI)

51.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuBisCO_small PF00101 1 - 52 7.3e-20 Ribulose bisphosphate carboxylase, small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0024250)

Species Orthologous Gene IDs
malus_domestica MD13G1041800.v1.1
pyrus_communis pycom13g03620
rosa_multiflora Rmu_co8397811.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 93
AciI CCGC 1 cut(s) 118
AclWI GGATC 1 cut(s) 104
AfiI CCNNNNNNNGG 2 cut(s) 20, 93
AjnI CCWGG 1 cut(s) 157
AluBI AGCT 2 cut(s) 10, 55
AluI AGCT 2 cut(s) 10, 55
Alw21I GWGCWC 1 cut(s) 57
AlwI GGATC 1 cut(s) 104
Ama87I CYCGRG 1 cut(s) 56
Aor13HI TCCGGA 1 cut(s) 93
AoxI GGCC 1 cut(s) 63
AvaI CYCGRG 1 cut(s) 56
BanII GRGCYC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 57
BciT130I CCWGG 1 cut(s) 159
Bme1390I CCNGG 1 cut(s) 159
BmeT110I CYCGRG 1 cut(s) 56
BmrFI CCNGG 1 cut(s) 159
BmsI GCATC 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 157
BsaWI WCCGGW 1 cut(s) 93
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 93
BseAI TCCGGA 1 cut(s) 93
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 157
BseGI GGATG 3 cut(s) 29, 90, 114
BseLI CCNNNNNNNGG 2 cut(s) 20, 93
BseRI GAGGAG 1 cut(s) 74
BshFI GGCC 1 cut(s) 65
BsiHKAI GWGCWC 1 cut(s) 57
BsiHKCI CYCGRG 1 cut(s) 56
BsiSI CCGG 1 cut(s) 94
BslI CCNNNNNNNGG 2 cut(s) 20, 93
BsnI GGCC 1 cut(s) 65
BsoBI CYCGRG 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 57
Bsp13I TCCGGA 1 cut(s) 93
Bsp143I GATC 1 cut(s) 96
BspACI CCGC 1 cut(s) 118
BspANI GGCC 1 cut(s) 65
BspEI TCCGGA 1 cut(s) 93
BspPI GGATC 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 157
BssMI GATC 1 cut(s) 96
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 1 cut(s) 129
Bst6I CTCTTC 1 cut(s) 23
BstF5I GGATG 3 cut(s) 29, 90, 114
BstKTI GATC 1 cut(s) 99
BstMBI GATC 1 cut(s) 96
BstNI CCWGG 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 157
BsuRI GGCC 1 cut(s) 65
BtgZI GCGATG 1 cut(s) 127
BtsCI GGATG 3 cut(s) 29, 90, 114
BtsIMutI CAGTG 2 cut(s) 27, 134
CseI GACGC 1 cut(s) 121
CviJI RGCY 5 cut(s) 10, 55, 65, 154, 162
CviKI_1 RGCY 5 cut(s) 10, 55, 65, 154, 162
DpnI GATC 1 cut(s) 98
DpnII GATC 1 cut(s) 96
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
EciI GGCGGA 1 cut(s) 107
Ecl136II GAGCTC 1 cut(s) 55
Eco24I GRGCYC 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 55
Eco88I CYCGRG 1 cut(s) 56
EcoICRI GAGCTC 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 157
EcoT38I GRGCYC 1 cut(s) 57
FokI GGATG 3 cut(s) 36, 77, 101
FriOI GRGCYC 1 cut(s) 57
HaeIII GGCC 1 cut(s) 65
HapII CCGG 1 cut(s) 94
HgaI GACGC 1 cut(s) 121
HindIII AAGCTT 1 cut(s) 8
HinfI GANTC 3 cut(s) 32, 83, 105
HpaII CCGG 1 cut(s) 94
Hpy188I TCNGA 2 cut(s) 23, 104
Hpy188III TCNNGA 1 cut(s) 94
HpyAV CCTTC 1 cut(s) 97
HpyCH4III ACNGT 1 cut(s) 129
HpyCH4V TGCA 1 cut(s) 27
Kpn2I TCCGGA 1 cut(s) 93
Kzo9I GATC 1 cut(s) 96
LmnI GCTCC 1 cut(s) 52
LpnPI CCDG 2 cut(s) 107, 144
LweI GCATC 1 cut(s) 14
MalI GATC 1 cut(s) 98
MboI GATC 1 cut(s) 96
MboII GAAGA 2 cut(s) 10, 27
MhlI GDGCHC 1 cut(s) 57
MnlI CCTC 3 cut(s) 26, 52, 55
MroI TCCGGA 1 cut(s) 93
MspI CCGG 1 cut(s) 94
MspR9I CCNGG 1 cut(s) 159
MvaI CCWGG 1 cut(s) 159
NdeII GATC 1 cut(s) 96
PaeR7I CTCGAG 1 cut(s) 56
PfeI GAWTC 3 cut(s) 32, 83, 105
Psp124BI GAGCTC 1 cut(s) 57
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
PspXI VCTCGAGB 1 cut(s) 56
SacI GAGCTC 1 cut(s) 57
Sau3AI GATC 1 cut(s) 96
ScrFI CCNGG 1 cut(s) 159
SduI GDGCHC 1 cut(s) 57
SetI ASST 3 cut(s) 12, 57, 77
SfaNI GCATC 1 cut(s) 14
Sfr274I CTCGAG 1 cut(s) 56
SgeI CNNG 8 cut(s) 53, 61, 68, 70, 79, 92, 106, 163
SlaI CTCGAG 1 cut(s) 56
SmlI CTYRAG 2 cut(s) 47, 56
SmoI CTYRAG 2 cut(s) 47, 56
SsiI CCGC 1 cut(s) 118
SstI GAGCTC 1 cut(s) 57
StyD4I CCNGG 1 cut(s) 157
TaaI ACNGT 1 cut(s) 129
TaqI TCGA 2 cut(s) 57, 108
TfiI GAWTC 3 cut(s) 32, 83, 105
TscAI CASTG 2 cut(s) 34, 134
TspDTI ATGAA 2 cut(s) 79, 130
TspRI CASTG 2 cut(s) 34, 134
XhoI CTCGAG 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.