MD13G1099000.v1.1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0022310
Physical Location & Seq
Forward (+)
6996994 .. 6997798
805 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1099000.v1.1.491

Sequence Viewer

Length: 264 bp
ATGAGGCGGTTCAAGAATTTAACATGCGGCTCAAGGAGAGAGTTTTACGAGTCCTTAACTCAATGTTGTGGAAGCTACGGTGATTTCCCCGTCCATTGTGGGGTGAAAGCTATTGTTAATGGCACTGCAGCTGGAGCACCTTGTAGCAATCCTTCTCAGTACATTAGCTGGGATGGAGGGCACTATTCTGATGCTGCAAACCAGTGGATAGCCAACGGAATTATGGACCGCGCATTCTCTGATCATCCGGTTTCAATCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

88

Amino Acids

9.46

Weight (kDa)

6.79

Isoelectric Point (pI)

32.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022310)

Species Orthologous Gene IDs
malus_domestica MD09G1022000.v1.1 MD13G1099000.v1.1 MD16G1099700.v1.1
pyrus_communis pycom111g01840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 231
AciI CCGC 3 cut(s) 7, 27, 229
AcsI RAATTY 1 cut(s) 16
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 1 cut(s) 100
AgsI TTSAA 2 cut(s) 13, 255
AluBI AGCT 4 cut(s) 75, 110, 131, 168
AluI AGCT 4 cut(s) 75, 110, 131, 168
Alw21I GWGCWC 1 cut(s) 139
ApeKI GCWGC 2 cut(s) 128, 194
ApoI RAATTY 1 cut(s) 16
AspLEI GCGC 1 cut(s) 233
AspS9I GGNCC 1 cut(s) 226
AsuHPI GGTGA 2 cut(s) 92, 115
AvaII GGWCC 1 cut(s) 226
BaeGI GKGCMC 1 cut(s) 183
BarI GAAGNNNNNNTAC 2 cut(s) 136, 168
Bbv12I GWGCWC 1 cut(s) 139
BbvI GCAGC 2 cut(s) 140, 181
BccI CCATC 1 cut(s) 167
BclI TGATCA 1 cut(s) 241
BfmI CTRYAG 1 cut(s) 126
BisI GCNGC 3 cut(s) 28, 129, 195
BlsI GCNGC 3 cut(s) 29, 130, 196
Bme18I GGWCC 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 226
BmsI GCATC 1 cut(s) 181
BpmI CTGGAG 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 16
BsaWI WCCGGW 1 cut(s) 247
Bsc4I CCNNNNNNNGG 1 cut(s) 100
Bse1I ACTGG 1 cut(s) 202
BseGI GGATG 2 cut(s) 178, 244
BseLI CCNNNNNNNGG 1 cut(s) 100
BseMII CTCAG 1 cut(s) 170
BseNI ACTGG 1 cut(s) 202
BseSI GKGCMC 1 cut(s) 183
BseXI GCAGC 2 cut(s) 140, 181
BseYI CCCAGC 1 cut(s) 168
Bsh1236I CGCG 1 cut(s) 231
BsiHKAI GWGCWC 1 cut(s) 139
BsiSI CCGG 1 cut(s) 248
BslI CCNNNNNNNGG 1 cut(s) 100
BsmI GAATGC 1 cut(s) 233
Bsp1286I GDGCHC 2 cut(s) 139, 183
Bsp143I GATC 1 cut(s) 241
BspACI CCGC 3 cut(s) 7, 27, 229
BspCNI CTCAG 1 cut(s) 169
BspFNI CGCG 1 cut(s) 231
BspMAI CTGCAG 1 cut(s) 130
BsrI ACTGG 1 cut(s) 202
BssMI GATC 1 cut(s) 241
Bst4CI ACNGT 1 cut(s) 80
BstDEI CTNAG 1 cut(s) 156
BstF5I GGATG 2 cut(s) 178, 244
BstFNI CGCG 1 cut(s) 231
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 1 cut(s) 244
BstMBI GATC 1 cut(s) 241
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNSI RCATGY 1 cut(s) 27
BstSFI CTRYAG 1 cut(s) 126
BstSLI GKGCMC 1 cut(s) 183
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 2 cut(s) 140, 181
BtsCI GGATG 2 cut(s) 178, 244
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 2 cut(s) 123, 209
CfoI GCGC 1 cut(s) 233
Cfr13I GGNCC 1 cut(s) 226
Csp6I GTAC 1 cut(s) 160
CviAII CATG 1 cut(s) 24
CviJI RGCY 6 cut(s) 30, 75, 110, 131, 168, 212
CviKI_1 RGCY 6 cut(s) 30, 75, 110, 131, 168, 212
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 1 cut(s) 156
DpnI GATC 1 cut(s) 243
DpnII GATC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 226
FaeI CATG 1 cut(s) 27
FaiI YATR 2 cut(s) 25, 224
FatI CATG 1 cut(s) 23
FbaI TGATCA 1 cut(s) 241
Fnu4HI GCNGC 3 cut(s) 28, 129, 195
FokI GGATG 2 cut(s) 185, 231
Fsp4HI GCNGC 3 cut(s) 28, 129, 195
GlaI GCGC 1 cut(s) 232
GluI GCNGC 3 cut(s) 28, 129, 195
GsaI CCCAGC 1 cut(s) 172
GsuI CTGGAG 1 cut(s) 153
HapII CCGG 1 cut(s) 248
HhaI GCGC 1 cut(s) 233
Hin1II CATG 1 cut(s) 27
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HinfI GANTC 1 cut(s) 50
HpaII CCGG 1 cut(s) 248
HphI GGTGA 2 cut(s) 92, 115
Hpy188I TCNGA 2 cut(s) 190, 241
Hpy188III TCNNGA 1 cut(s) 13
HpyAV CCTTC 1 cut(s) 162
HpyCH4III ACNGT 1 cut(s) 80
HpyCH4V TGCA 2 cut(s) 128, 197
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 1 cut(s) 156
Hsp92II CATG 1 cut(s) 27
HspAI GCGC 1 cut(s) 231
Ksp22I TGATCA 1 cut(s) 241
Kzo9I GATC 1 cut(s) 241
LmnI GCTCC 1 cut(s) 134
LpnPI CCDG 3 cut(s) 117, 154, 215
Lsp1109I GCAGC 2 cut(s) 140, 181
LweI GCATC 1 cut(s) 181
MalI GATC 1 cut(s) 243
MboI GATC 1 cut(s) 241
MhlI GDGCHC 2 cut(s) 139, 183
MluCI AATT 2 cut(s) 16, 219
MlyI GAGTC 1 cut(s) 59
MnlI CCTC 1 cut(s) 170
MseI TTAA 4 cut(s) 20, 56, 117, 262
MspA1I CMGCKG 1 cut(s) 131
MspI CCGG 1 cut(s) 248
Mva1269I GAATGC 1 cut(s) 233
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 1 cut(s) 134
NdeII GATC 1 cut(s) 241
NlaIII CATG 1 cut(s) 27
NspI RCATGY 1 cut(s) 27
PctI GAATGC 1 cut(s) 233
PkrI GCNGC 3 cut(s) 29, 130, 196
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
PspFI CCCAGC 1 cut(s) 168
PspPI GGNCC 1 cut(s) 226
PstI CTGCAG 1 cut(s) 130
PvuII CAGCTG 1 cut(s) 131
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
SaqAI TTAA 4 cut(s) 20, 56, 117, 262
SatI GCNGC 3 cut(s) 28, 129, 195
Sau3AI GATC 1 cut(s) 241
Sau96I GGNCC 1 cut(s) 226
SchI GAGTC 1 cut(s) 59
SduI GDGCHC 2 cut(s) 139, 183
SetI ASST 5 cut(s) 77, 112, 133, 142, 170
SfaNI GCATC 1 cut(s) 181
SfcI CTRYAG 1 cut(s) 126
SinI GGWCC 1 cut(s) 226
SmlI CTYRAG 1 cut(s) 31
SmoI CTYRAG 1 cut(s) 31
Sse9I AATT 2 cut(s) 16, 219
SsiI CCGC 3 cut(s) 7, 27, 229
TaaI ACNGT 1 cut(s) 80
TasI AATT 2 cut(s) 16, 219
TatI WGTACW 1 cut(s) 159
TauI GCSGC 1 cut(s) 30
Tru1I TTAA 4 cut(s) 20, 56, 117, 262
Tru9I TTAA 4 cut(s) 20, 56, 117, 262
TscAI CASTG 2 cut(s) 130, 209
TseI GCWGC 2 cut(s) 128, 194
TspGWI ACGGA 1 cut(s) 231
TspRI CASTG 2 cut(s) 130, 209
VpaK11BI GGWCC 1 cut(s) 226
XapI RAATTY 1 cut(s) 16
XceI RCATGY 1 cut(s) 27
XcmI CCANNNNNNNNNTGG 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.