MD16G1099700.v1.1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0022310
Physical Location & Seq
Forward (+)
6936393 .. 6937011
619 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1099700.v1.1.491

Sequence Viewer

Length: 384 bp
ATGGGGAGTGAAGAGTGGAGGCAGTGGCAATTGGAGTGGTTGCCAAGTCCCATCAAGGTTTTAACTTTGGCGATTCTAATTCCAACACGGATGATTTATCAGCTACATTTTTCAACTTCCTTCTCCCTACCGCAATACATTCTTTGCAACTCTACCAACAAGGAGCAAGAGTTCATTGGATTCACAACACAGGTCCCCACGGCGGTCGGAGGAAAGCGATTGTTAACGGCACTGAAGTTGAGATGGGGTGGGGTGCACTATTCCGATGCTGCAAACAACTTGATAGCCAACCAAATTATGGGCGGCTCTTTCTCCGACTCTCCAGTCTCAATCGCTGAAGCATGTCGCAATCCTCCACGTTCTGATTCAACTTCAAGCGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

128

Amino Acids

14.07

Weight (kDa)

7.81

Isoelectric Point (pI)

56.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022310)

Species Orthologous Gene IDs
malus_domestica MD09G1022000.v1.1 MD13G1099000.v1.1 MD16G1099700.v1.1
pyrus_communis pycom111g01840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 323
AccB7I CCANNNNNTGG 1 cut(s) 298
AciI CCGC 4 cut(s) 131, 203, 303, 378
AcuI CTGAAG 2 cut(s) 254, 357
AfiI CCNNNNNNNGG 2 cut(s) 202, 298
AgsI TTSAA 3 cut(s) 114, 369, 375
AloI GAACNNNNNNTCC 2 cut(s) 155, 187
AluBI AGCT 1 cut(s) 103
AluI AGCT 1 cut(s) 103
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 1 cut(s) 331
Alw44I GTGCAC 1 cut(s) 254
ApaLI GTGCAC 1 cut(s) 254
ApeKI GCWGC 1 cut(s) 269
AspS9I GGNCC 1 cut(s) 193
AvaII GGWCC 1 cut(s) 193
BaeGI GKGCMC 1 cut(s) 258
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 1 cut(s) 256
BccI CCATC 2 cut(s) 59, 237
BceAI ACGGC 2 cut(s) 216, 243
BcoDI GTCTC 1 cut(s) 331
BisI GCNGC 2 cut(s) 270, 304
BlsI GCNGC 2 cut(s) 271, 305
Bme18I GGWCC 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 1 cut(s) 195
BmsI GCATC 1 cut(s) 256
BpmI CTGGAG 1 cut(s) 306
BsaJI CCNNGG 1 cut(s) 198
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 298
Bse1I ACTGG 1 cut(s) 323
BseDI CCNNGG 1 cut(s) 198
BseGI GGATG 1 cut(s) 96
BseLI CCNNNNNNNGG 2 cut(s) 202, 298
BseNI ACTGG 1 cut(s) 323
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 1 cut(s) 256
Bsh1285I CGRYCG 1 cut(s) 207
BsiEI CGRYCG 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 258
BslFI GGGAC 2 cut(s) 33, 179
BslI CCNNNNNNNGG 2 cut(s) 202, 298
BsmAI GTCTC 1 cut(s) 331
BsmFI GGGAC 2 cut(s) 33, 179
Bsp1286I GDGCHC 1 cut(s) 258
BspACI CCGC 4 cut(s) 131, 203, 303, 378
BspLI GGNNCC 1 cut(s) 195
BsrI ACTGG 1 cut(s) 323
BssECI CCNNGG 1 cut(s) 198
Bst6I CTCTTC 1 cut(s) 6
BstDSI CCRYGG 1 cut(s) 198
BstF5I GGATG 1 cut(s) 96
BstMAI GTCTC 1 cut(s) 331
BstMCI CGRYCG 1 cut(s) 207
BstNSI RCATGY 1 cut(s) 345
BstSLI GKGCMC 1 cut(s) 258
BstV1I GCAGC 1 cut(s) 256
BtgI CCRYGG 1 cut(s) 198
BtsCI GGATG 1 cut(s) 96
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 2 cut(s) 29, 230
Cfr13I GGNCC 1 cut(s) 193
CspCI CAANNNNNGTGG 2 cut(s) 17, 52
CviAII CATG 1 cut(s) 342
CviJI RGCY 3 cut(s) 103, 287, 306
CviKI_1 RGCY 3 cut(s) 103, 287, 306
DrdI GACNNNNNNGTC 1 cut(s) 323
DseDI GACNNNNNNGTC 1 cut(s) 323
Eam1104I CTCTTC 1 cut(s) 6
EarI CTCTTC 1 cut(s) 6
Eco47I GGWCC 1 cut(s) 193
Eco57I CTGAAG 2 cut(s) 254, 357
EcoO109I RGGNCCY 1 cut(s) 193
FaeI CATG 1 cut(s) 345
FaiI YATR 2 cut(s) 299, 343
FaqI GGGAC 2 cut(s) 33, 179
FatI CATG 1 cut(s) 341
Fnu4HI GCNGC 2 cut(s) 270, 304
FokI GGATG 1 cut(s) 103
Fsp4HI GCNGC 2 cut(s) 270, 304
GluI GCNGC 2 cut(s) 270, 304
GsuI CTGGAG 1 cut(s) 306
Hin1II CATG 1 cut(s) 345
HincII GTYRAC 1 cut(s) 225
HindII GTYRAC 1 cut(s) 225
HinfI GANTC 4 cut(s) 73, 180, 317, 365
HpaI GTTAAC 1 cut(s) 225
Hpy166II GTNNAC 2 cut(s) 225, 256
Hpy188I TCNGA 4 cut(s) 209, 265, 316, 364
Hpy8I GTNNAC 2 cut(s) 225, 256
HpyAV CCTTC 1 cut(s) 130
HpyCH4IV ACGT 1 cut(s) 358
HpyCH4V TGCA 3 cut(s) 147, 256, 272
HpySE526I ACGT 1 cut(s) 358
Hsp92II CATG 1 cut(s) 345
KspAI GTTAAC 1 cut(s) 225
LmnI GCTCC 1 cut(s) 163
LpnPI CCDG 2 cut(s) 176, 336
Lsp1109I GCAGC 1 cut(s) 256
LweI GCATC 1 cut(s) 256
MaeII ACGT 1 cut(s) 358
MboII GAAGA 1 cut(s) 23
MfeI CAATTG 1 cut(s) 29
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 3 cut(s) 29, 78, 294
MlyI GAGTC 1 cut(s) 311
MmeI TCCRAC 3 cut(s) 107, 187, 339
MnlI CCTC 3 cut(s) 12, 203, 363
MseI TTAA 2 cut(s) 62, 224
MunI CAATTG 1 cut(s) 29
NlaIII CATG 1 cut(s) 345
NlaIV GGNNCC 1 cut(s) 195
NspI RCATGY 1 cut(s) 345
PfeI GAWTC 3 cut(s) 73, 180, 365
PflMI CCANNNNNTGG 1 cut(s) 298
PkrI GCNGC 2 cut(s) 271, 305
PleI GAGTC 1 cut(s) 311
PpsI GAGTC 1 cut(s) 311
PpuMI RGGWCCY 1 cut(s) 193
Psp5II RGGWCCY 1 cut(s) 193
PspN4I GGNNCC 1 cut(s) 195
PspPI GGNCC 1 cut(s) 193
PspPPI RGGWCCY 1 cut(s) 193
SaqAI TTAA 2 cut(s) 62, 224
SatI GCNGC 2 cut(s) 270, 304
Sau96I GGNCC 1 cut(s) 193
SchI GAGTC 1 cut(s) 311
SduI GDGCHC 1 cut(s) 258
SetI ASST 4 cut(s) 60, 105, 195, 361
SfaNI GCATC 1 cut(s) 256
SinI GGWCC 1 cut(s) 193
Sse9I AATT 3 cut(s) 29, 78, 294
SsiI CCGC 4 cut(s) 131, 203, 303, 378
TaiI ACGT 1 cut(s) 361
TasI AATT 3 cut(s) 29, 78, 294
TauI GCSGC 1 cut(s) 306
TfiI GAWTC 3 cut(s) 73, 180, 365
Tru1I TTAA 2 cut(s) 62, 224
Tru9I TTAA 2 cut(s) 62, 224
TscAI CASTG 2 cut(s) 29, 237
TseI GCWGC 1 cut(s) 269
TspDTI ATGAA 1 cut(s) 163
TspGWI ACGGA 1 cut(s) 103
TspRI CASTG 2 cut(s) 29, 237
Van91I CCANNNNNTGG 1 cut(s) 298
VneI GTGCAC 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 193
XceI RCATGY 1 cut(s) 345
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.