MD13G1246600.v1.1

Phosphoribulokinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0024240
Physical Location & Seq
Forward (+)
25979671 .. 25980515
845 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1246600.v1.1.491

Sequence Viewer

Length: 345 bp
ATGGCTTCAGATTGGATGAACAAACAAACGGCGGAGATCTCGAGGTTTGCGGATTCAAGAATGATAAGGCTGTTTGACTCCAACATGCTCATCAGCAACACCACGGCGGTGATATGCTTGGATGATTACCATTCCCTAGACATGACATGGAGGAAGGAGAAAGAAGTGACGATGCTAGACCCAAGAGCTACCTACTTCGATCTTATGTATAAGCAGATGAAGGCAATCAAGGAAGGGAAGGCTGTTGAGAAGCCTATTTACACAATGTTTCTGGTCGCTTGGATCCTCCTGAGCTCATCAAGTTCCCCCAAGATTCTTGTCATTGAAGGTTTACGTGCAATGTAA

Protein Analysis

115

Amino Acids

13.22

Weight (kDa)

7.82

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRK PF00485 29 - 113 2.4e-15 Phosphoribulokinase / Uridine kinase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024240)

Species Orthologous Gene IDs
malus_domestica MD11G1196800.v1.1 MD13G1246600.v1.1
pyrus_communis pycom11g16980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 32, 50, 107
AclWI GGATC 2 cut(s) 277, 290
AgsI TTSAA 2 cut(s) 57, 326
AleI CACNNNNGTG 1 cut(s) 107
AluBI AGCT 2 cut(s) 188, 294
AluI AGCT 2 cut(s) 188, 294
Alw21I GWGCWC 1 cut(s) 296
AlwI GGATC 2 cut(s) 277, 290
Ama87I CYCGRG 1 cut(s) 40
AsuHPI GGTGA 1 cut(s) 121
AvaI CYCGRG 1 cut(s) 40
BamHI GGATCC 1 cut(s) 282
BanII GRGCYC 1 cut(s) 296
BarI GAAGNNNNNNTAC 2 cut(s) 242, 274
Bbv12I GWGCWC 1 cut(s) 296
BceAI ACGGC 2 cut(s) 45, 120
BfaI CTAG 2 cut(s) 137, 176
BglII AGATCT 1 cut(s) 36
BmeT110I CYCGRG 1 cut(s) 40
BmiI GGNNCC 1 cut(s) 284
BmsI GCATC 1 cut(s) 162
Bpu10I CCTNAGC 1 cut(s) 290
BsaAI YACGTR 1 cut(s) 335
BsaJI CCNNGG 1 cut(s) 102
Bse3DI GCAATG 1 cut(s) 345
BseDI CCNNGG 1 cut(s) 102
BseGI GGATG 2 cut(s) 21, 127
BseMI GCAATG 1 cut(s) 345
BseMII CTCAG 1 cut(s) 281
BsiHKAI GWGCWC 1 cut(s) 296
BsiHKCI CYCGRG 1 cut(s) 40
BsoBI CYCGRG 1 cut(s) 40
Bsp1286I GDGCHC 1 cut(s) 296
Bsp143I GATC 3 cut(s) 36, 199, 282
BspACI CCGC 3 cut(s) 32, 50, 107
BspCNI CTCAG 1 cut(s) 282
BspLI GGNNCC 1 cut(s) 284
BspPI GGATC 2 cut(s) 277, 290
BsrDI GCAATG 1 cut(s) 345
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 3 cut(s) 36, 199, 282
BstBAI YACGTR 1 cut(s) 335
BstDEI CTNAG 1 cut(s) 290
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 2 cut(s) 21, 127
BstKTI GATC 3 cut(s) 39, 202, 285
BstMBI GATC 3 cut(s) 36, 199, 282
BstNSI RCATGY 1 cut(s) 88
BstX2I RGATCY 2 cut(s) 36, 282
BstYI RGATCY 2 cut(s) 36, 282
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 2 cut(s) 21, 127
CviAII CATG 3 cut(s) 85, 142, 147
CviJI RGCY 6 cut(s) 5, 70, 188, 242, 253, 294
CviKI_1 RGCY 6 cut(s) 5, 70, 188, 242, 253, 294
DdeI CTNAG 1 cut(s) 290
DpnI GATC 3 cut(s) 38, 201, 284
DpnII GATC 3 cut(s) 36, 199, 282
EciI GGCGGA 1 cut(s) 47
Ecl136II GAGCTC 1 cut(s) 294
Eco24I GRGCYC 1 cut(s) 296
Eco53kI GAGCTC 1 cut(s) 294
Eco88I CYCGRG 1 cut(s) 40
EcoICRI GAGCTC 1 cut(s) 294
EcoT38I GRGCYC 1 cut(s) 296
FaeI CATG 3 cut(s) 88, 145, 150
FaiI YATR 6 cut(s) 86, 115, 143, 148, 206, 210
FatI CATG 3 cut(s) 84, 141, 146
FokI GGATG 2 cut(s) 28, 134
FriOI GRGCYC 1 cut(s) 296
FspBI CTAG 2 cut(s) 137, 176
Hin1II CATG 3 cut(s) 88, 145, 150
HinfI GANTC 3 cut(s) 53, 77, 313
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 1 cut(s) 332
Hpy188I TCNGA 1 cut(s) 10
Hpy188III TCNNGA 3 cut(s) 40, 57, 289
Hpy8I GTNNAC 1 cut(s) 332
HpyAV CCTTC 5 cut(s) 148, 214, 227, 232, 320
HpyCH4IV ACGT 1 cut(s) 334
HpyCH4V TGCA 1 cut(s) 338
HpyF3I CTNAG 1 cut(s) 290
HpySE526I ACGT 1 cut(s) 334
Hsp92II CATG 3 cut(s) 88, 145, 150
Kzo9I GATC 3 cut(s) 36, 199, 282
LpnPI CCDG 2 cut(s) 257, 302
LweI GCATC 1 cut(s) 162
MaeI CTAG 2 cut(s) 137, 176
MaeII ACGT 1 cut(s) 334
MaeIII GTNAC 1 cut(s) 166
MalI GATC 3 cut(s) 38, 201, 284
MboI GATC 3 cut(s) 36, 199, 282
MflI RGATCY 2 cut(s) 36, 282
MhlI GDGCHC 1 cut(s) 296
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 1 cut(s) 105
MnlI CCTC 3 cut(s) 36, 144, 296
MslI CAYNNNNRTG 1 cut(s) 107
NdeII GATC 3 cut(s) 36, 199, 282
NlaIII CATG 3 cut(s) 88, 145, 150
NlaIV GGNNCC 1 cut(s) 284
NmuCI GTSAC 1 cut(s) 166
NspI RCATGY 1 cut(s) 88
OliI CACNNNNGTG 1 cut(s) 107
PaeR7I CTCGAG 1 cut(s) 40
PfeI GAWTC 2 cut(s) 53, 313
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
Ppu21I YACGTR 1 cut(s) 335
Psp124BI GAGCTC 1 cut(s) 296
PspN4I GGNNCC 1 cut(s) 284
PsuI RGATCY 2 cut(s) 36, 282
RseI CAYNNNNRTG 1 cut(s) 107
SacI GAGCTC 1 cut(s) 296
Sau3AI GATC 3 cut(s) 36, 199, 282
SchI GAGTC 1 cut(s) 71
SduI GDGCHC 1 cut(s) 296
SetI ASST 6 cut(s) 47, 190, 194, 296, 331, 337
SfaNI GCATC 1 cut(s) 162
Sfr274I CTCGAG 1 cut(s) 40
SlaI CTCGAG 1 cut(s) 40
SmiMI CAYNNNNRTG 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 40
SmoI CTYRAG 1 cut(s) 40
SsiI CCGC 3 cut(s) 32, 50, 107
SspMI CTAG 2 cut(s) 137, 176
SstI GAGCTC 1 cut(s) 296
TaiI ACGT 1 cut(s) 337
TaqI TCGA 2 cut(s) 41, 198
TfiI GAWTC 2 cut(s) 53, 313
TseFI GTSAC 1 cut(s) 166
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 2 cut(s) 32, 233
XceI RCATGY 1 cut(s) 88
XhoI CTCGAG 1 cut(s) 40
XspI CTAG 2 cut(s) 137, 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.