MD14G1165900.v1.1

domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0023575
Physical Location & Seq
Reverse (-)
25954581 .. 25957732
3152 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1165900.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGGGTGGGGTTACTTCATCCATGGCTGCAAAGTTTGCATTTTTTCCACCGAACCCACCATCCTACAAGGTAGTGAAAGACGAAGCCACAGGGCTGTTGCTACTGGATCCGCTTCCCCACCGTGAAAACGTCGACGTTTTGAAGTTTCCGACGCGCCGCGGCAATGAGATCGTCGCCGTCTATATTCGCCACCCAATGGCCACGTCCACGCTCCTTTACTCCCACGGCAACGCCACCGATATCGGCCAGATGTACGAGCTTTTTATCGAATTGAGCATTCACCTGCGCGTCAATCTCATGGCGTATGATTACTCTGGCTATGGGCAGTCCTCAGGCAAGGCAAGTGAACATAATACTTATGCAGATATTGAAGCTGCATATAAGTGTCTTGAAGAGAGGTATGGTGCTAAGCAGGAAGACGTAATCCTCTATGGTCAGTCGGTTGGAAGTGGCCCGACTGTTGATCTTGCTGTCCGTTTGCCTCGATTAAGGGCAATTGTGCTGCATAGTCCTATATTATCTGGTTTAAGAGTCATGTATCCTGTGAAGCGCACATACTGGTTTGATATCTATAAGAACATTGATAAAATCCCACTGGTAAAATGTCCTGTGCTGGTAATACACGGAACATCTGATGAAGTTGTGGACTGCTCACACGGCAAGCAACTTTGGGTACTTTGCCAAGAGAAATATGAACCTCTATGGCTCAAAGGCGGAAATCACTGTAATTTGGAGCTCTATCCAGAATATATTAGACATCTCAACAAGTTCATCTCAACTGTCGAAAAATCACCTTCACGTAGGATTACTTCAAGAAAAAGCACAGGCCACAGGAAAAGCACAGACCGTATTGAACATCCTAGGAGGAGTGTCGATTTTTATGAGGCTCCAAGAAAGAGTACTGATCGGAGAGAGAAATCAAGGAAAAGCACTGATAGACCTGAAAAGCTGAATATTAGTGAATACAAGTTCAATAACATCGACAAGGTAGACAAGTTTAGAATCTCTGTTGACCAGATAGAGAAGTCACGAAGAAGTGTGGAATACCGTCATGAGAAATCCAGGAGAAGCGTCGACTGTCAGCTAGAAAAACCACGGAAGAGCGTTGACTGGCTGGACAGAATTCGACCTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

379

Amino Acids

43.62

Weight (kDa)

9.39

Isoelectric Point (pI)

51.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 70 - 175 1.1e-07 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023575)

Species Orthologous Gene IDs
malus_domestica MD14G1165900.v1.1
prunus_persica Prupe.5G160600_v2.0.a1
pyrus_communis pycom14g13850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 291
Acc36I ACCTGC 1 cut(s) 291
AccB7I CCANNNNNTGG 1 cut(s) 196
AccI GTMKAC 3 cut(s) 132, 990, 1074
AccII CGCG 3 cut(s) 154, 159, 288
AciI CCGC 4 cut(s) 110, 157, 159, 714
AclWI GGATC 2 cut(s) 101, 114
AcoI YGGCCR 2 cut(s) 198, 244
AcsI RAATTY 1 cut(s) 1122
AfaI GTAC 3 cut(s) 254, 675, 901
AfiI CCNNNNNNNGG 1 cut(s) 196
AgsI TTSAA 6 cut(s) 142, 371, 392, 813, 854, 973
AjiI CACGTC 1 cut(s) 204
AjnI CCWGG 2 cut(s) 1061, 1129
AloI GAACNNNNNNTCC 2 cut(s) 44, 76
AluBI AGCT 5 cut(s) 259, 374, 736, 949, 1084
AluI AGCT 5 cut(s) 259, 374, 736, 949, 1084
Alw21I GWGCWC 1 cut(s) 738
AlwI GGATC 2 cut(s) 101, 114
AoxI GGCC 4 cut(s) 198, 244, 451, 826
ApeKI GCWGC 3 cut(s) 26, 374, 502
ApoI RAATTY 1 cut(s) 1122
AspA2I CCTAGG 1 cut(s) 860
AspLEI GCGC 3 cut(s) 156, 288, 552
AspS9I GGNCC 1 cut(s) 452
AsuHPI GGTGA 2 cut(s) 272, 783
AvrII CCTAGG 1 cut(s) 860
AxyI CCTNAGG 1 cut(s) 331
BalI TGGCCA 1 cut(s) 200
BamHI GGATCC 1 cut(s) 106
BanII GRGCYC 1 cut(s) 738
BarI GAAGNNNNNNTAC 4 cut(s) 539, 571, 793, 825
BbsI GAAGAC 1 cut(s) 423
Bbv12I GWGCWC 1 cut(s) 738
BbvI GCAGC 3 cut(s) 13, 361, 489
BccI CCATC 1 cut(s) 67
BceAI ACGGC 3 cut(s) 161, 241, 673
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BciT130I CCWGG 2 cut(s) 1063, 1131
BciVI GTATCC 1 cut(s) 549
BfaI CTAG 2 cut(s) 861, 1085
BfuAI ACCTGC 1 cut(s) 291
BfuI GTATCC 1 cut(s) 549
BisI GCNGC 5 cut(s) 27, 157, 160, 375, 503
BlnI CCTAGG 1 cut(s) 860
BlpI GCTNAGC 1 cut(s) 408
BlsI GCNGC 5 cut(s) 28, 158, 161, 376, 504
BmcAI AGTACT 1 cut(s) 901
Bme1390I CCNGG 2 cut(s) 1063, 1131
BmgBI CACGTC 1 cut(s) 204
BmgT120I GGNCC 1 cut(s) 452
BmiI GGNNCC 2 cut(s) 108, 888
BmrFI CCNGG 2 cut(s) 1063, 1131
BpiI GAAGAC 1 cut(s) 423
Bpu1102I GCTNAGC 1 cut(s) 408
BsaAI YACGTR 1 cut(s) 800
BsaJI CCNNGG 6 cut(s) 21, 157, 223, 860, 1094, 1130
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse1I ACTGG 4 cut(s) 108, 563, 600, 1115
Bse21I CCTNAGG 1 cut(s) 331
Bse3DI GCAATG 1 cut(s) 169
BseBI CCWGG 2 cut(s) 1063, 1131
BseDI CCNNGG 6 cut(s) 21, 157, 223, 860, 1094, 1130
BseGI GGATG 3 cut(s) 17, 59, 856
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 4 cut(s) 108, 563, 600, 1115
BseRI GAGGAG 1 cut(s) 880
BseXI GCAGC 3 cut(s) 13, 361, 489
Bsh1236I CGCG 3 cut(s) 154, 159, 288
BshFI GGCC 4 cut(s) 200, 246, 453, 828
BsiHKAI GWGCWC 1 cut(s) 738
BslI CCNNNNNNNGG 1 cut(s) 196
BsmI GAATGC 1 cut(s) 276
BsnI GGCC 4 cut(s) 200, 246, 453, 828
Bsp1286I GDGCHC 1 cut(s) 738
Bsp143I GATC 4 cut(s) 106, 168, 463, 904
Bsp1720I GCTNAGC 1 cut(s) 408
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 4 cut(s) 110, 157, 159, 714
BspANI GGCC 4 cut(s) 200, 246, 453, 828
BspCNI CTCAG 1 cut(s) 344
BspFNI CGCG 3 cut(s) 154, 159, 288
BspHI TCATGA 1 cut(s) 1051
BspLI GGNNCC 2 cut(s) 108, 888
BspMI ACCTGC 1 cut(s) 291
BspPI GGATC 2 cut(s) 101, 114
BspQI GCTCTTC 1 cut(s) 1094
BsrDI GCAATG 1 cut(s) 169
BsrI ACTGG 4 cut(s) 108, 563, 600, 1115
BssECI CCNNGG 6 cut(s) 21, 157, 223, 860, 1094, 1130
BssMI GATC 4 cut(s) 106, 168, 463, 904
BssT1I CCWWGG 2 cut(s) 21, 860
Bst2UI CCWGG 2 cut(s) 1063, 1131
Bst4CI ACNGT 7 cut(s) 122, 460, 725, 781, 848, 1049, 1079
Bst6I CTCTTC 2 cut(s) 387, 1094
BstAPI GCANNNNNTGC 1 cut(s) 35
BstBAI YACGTR 1 cut(s) 800
BstC8I GCNNGC 1 cut(s) 662
BstDEI CTNAG 2 cut(s) 331, 408
BstDSI CCRYGG 4 cut(s) 21, 157, 223, 1094
BstF5I GGATG 3 cut(s) 17, 59, 856
BstFNI CGCG 3 cut(s) 154, 159, 288
BstHHI GCGC 3 cut(s) 156, 288, 552
BstKTI GATC 4 cut(s) 109, 171, 466, 907
BstMBI GATC 4 cut(s) 106, 168, 463, 904
BstMWI GCNNNNNNNGC 2 cut(s) 35, 657
BstNI CCWGG 2 cut(s) 1063, 1131
BstSCI CCNGG 2 cut(s) 1061, 1129
BstUI CGCG 3 cut(s) 154, 159, 288
BstV1I GCAGC 3 cut(s) 13, 361, 489
BstV2I GAAGAC 1 cut(s) 423
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
Bsu36I CCTNAGG 1 cut(s) 331
BsuI GTATCC 1 cut(s) 549
BsuRI GGCC 4 cut(s) 200, 246, 453, 828
BtgI CCRYGG 4 cut(s) 21, 157, 223, 1094
BtrI CACGTC 1 cut(s) 204
BtsCI GGATG 3 cut(s) 17, 59, 856
BtsIMutI CAGTG 3 cut(s) 593, 721, 930
BveI ACCTGC 1 cut(s) 291
Cac8I GCNNGC 1 cut(s) 662
CciI TCATGA 1 cut(s) 1051
CfoI GCGC 3 cut(s) 156, 288, 552
Cfr13I GGNCC 1 cut(s) 452
Cfr42I CCGCGG 1 cut(s) 160
CseI GACGC 3 cut(s) 160, 277, 1060
Csp6I GTAC 3 cut(s) 253, 674, 900
CviAII CATG 4 cut(s) 22, 298, 535, 1052
CviQI GTAC 3 cut(s) 253, 674, 900
DdeI CTNAG 2 cut(s) 331, 408
DpnI GATC 4 cut(s) 108, 170, 465, 906
DpnII GATC 4 cut(s) 106, 168, 463, 904
EaeI YGGCCR 2 cut(s) 198, 244
Eam1104I CTCTTC 2 cut(s) 387, 1094
EarI CTCTTC 2 cut(s) 387, 1094
EciI GGCGGA 1 cut(s) 729
Ecl136II GAGCTC 1 cut(s) 736
Eco130I CCWWGG 2 cut(s) 21, 860
Eco24I GRGCYC 1 cut(s) 738
Eco32I GATATC 2 cut(s) 241, 568
Eco53kI GAGCTC 1 cut(s) 736
Eco81I CCTNAGG 1 cut(s) 331
EcoICRI GAGCTC 1 cut(s) 736
EcoRI GAATTC 1 cut(s) 1122
EcoRII CCWGG 2 cut(s) 1061, 1129
EcoRV GATATC 2 cut(s) 241, 568
EcoT14I CCWWGG 2 cut(s) 21, 860
EcoT38I GRGCYC 1 cut(s) 738
ErhI CCWWGG 2 cut(s) 21, 860
FaeI CATG 4 cut(s) 25, 301, 538, 1055
FatI CATG 4 cut(s) 21, 297, 534, 1051
FblI GTMKAC 3 cut(s) 132, 990, 1074
Fnu4HI GCNGC 5 cut(s) 27, 157, 160, 375, 503
FokI GGATG 3 cut(s) 4, 46, 843
FriOI GRGCYC 1 cut(s) 738
Fsp4HI GCNGC 5 cut(s) 27, 157, 160, 375, 503
FspBI CTAG 2 cut(s) 861, 1085
GlaI GCGC 3 cut(s) 155, 287, 551
GluI GCNGC 5 cut(s) 27, 157, 160, 375, 503
HaeIII GGCC 4 cut(s) 200, 246, 453, 828
HgaI GACGC 3 cut(s) 160, 277, 1060
HhaI GCGC 3 cut(s) 156, 288, 552
Hin1II CATG 4 cut(s) 25, 301, 538, 1055
Hin6I GCGC 3 cut(s) 154, 286, 550
HinP1I GCGC 3 cut(s) 154, 286, 550
HincII GTYRAC 4 cut(s) 133, 1012, 1075, 1108
HindII GTYRAC 4 cut(s) 133, 1012, 1075, 1108
HinfI GANTC 2 cut(s) 531, 1002
HphI GGTGA 2 cut(s) 272, 783
Hpy166II GTNNAC 8 cut(s) 133, 207, 347, 646, 991, 1012, 1075, 1108
Hpy188I TCNGA 3 cut(s) 150, 634, 909
Hpy188III TCNNGA 5 cut(s) 389, 743, 813, 1029, 1052
Hpy8I GTNNAC 8 cut(s) 133, 207, 347, 646, 991, 1012, 1075, 1108
Hpy99I CGWCG 5 cut(s) 134, 137, 154, 176, 1076
HpyAV CCTTC 1 cut(s) 804
HpyCH4III ACNGT 7 cut(s) 122, 460, 725, 781, 848, 1049, 1079
HpyCH4IV ACGT 5 cut(s) 129, 135, 203, 420, 799
HpyCH4V TGCA 5 cut(s) 29, 38, 362, 377, 505
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 657
HpyF3I CTNAG 2 cut(s) 331, 408
HpySE526I ACGT 5 cut(s) 129, 135, 203, 420, 799
Hsp92II CATG 4 cut(s) 25, 301, 538, 1055
HspAI GCGC 3 cut(s) 154, 286, 550
KspI CCGCGG 1 cut(s) 160
Kzo9I GATC 4 cut(s) 106, 168, 463, 904
LguI GCTCTTC 1 cut(s) 1094
LmnI GCTCC 3 cut(s) 216, 733, 892
Lsp1109I GCAGC 3 cut(s) 13, 361, 489
MaeI CTAG 2 cut(s) 861, 1085
MaeII ACGT 5 cut(s) 129, 135, 203, 420, 799
MaeIII GTNAC 2 cut(s) 10, 1026
MalI GATC 4 cut(s) 108, 170, 465, 906
MboI GATC 4 cut(s) 106, 168, 463, 904
MboII GAAGA 4 cut(s) 404, 428, 1044, 1111
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 1 cut(s) 106
MhlI GDGCHC 1 cut(s) 738
MlsI TGGCCA 1 cut(s) 200
MluCI AATT 4 cut(s) 269, 495, 727, 1122
MluNI TGGCCA 1 cut(s) 200
MlyI GAGTC 1 cut(s) 540
MmeI TCCRAC 2 cut(s) 173, 424
MnlI CCTC 7 cut(s) 340, 390, 437, 492, 708, 858, 877
Mox20I TGGCCA 1 cut(s) 200
MscI TGGCCA 1 cut(s) 200
MseI TTAA 2 cut(s) 488, 527
MslI CAYNNNNRTG 1 cut(s) 382
Msp20I TGGCCA 1 cut(s) 200
MspA1I CMGCKG 1 cut(s) 159
MspR9I CCNGG 2 cut(s) 1063, 1131
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 276
MvaI CCWGG 2 cut(s) 1063, 1131
MvnI CGCG 3 cut(s) 154, 159, 288
MwoI GCNNNNNNNGC 2 cut(s) 35, 657
NcoI CCATGG 1 cut(s) 21
NdeII GATC 4 cut(s) 106, 168, 463, 904
NlaIII CATG 4 cut(s) 25, 301, 538, 1055
NlaIV GGNNCC 2 cut(s) 108, 888
NmuCI GTSAC 1 cut(s) 1026
PagI TCATGA 1 cut(s) 1051
PaqCI CACCTGC 1 cut(s) 291
PciSI GCTCTTC 1 cut(s) 1094
PctI GAATGC 1 cut(s) 276
PfeI GAWTC 1 cut(s) 1002
PflMI CCANNNNNTGG 1 cut(s) 196
PfoI TCCNGGA 1 cut(s) 1061
PkrI GCNGC 5 cut(s) 28, 158, 161, 376, 504
PleI GAGTC 1 cut(s) 539
PpsI GAGTC 1 cut(s) 539
Ppu21I YACGTR 1 cut(s) 800
Psp124BI GAGCTC 1 cut(s) 738
Psp6I CCWGG 2 cut(s) 1061, 1129
PspGI CCWGG 2 cut(s) 1061, 1129
PspN4I GGNNCC 2 cut(s) 108, 888
PspPI GGNCC 1 cut(s) 452
PsuI RGATCY 1 cut(s) 106
RsaI GTAC 3 cut(s) 254, 675, 901
RsaNI GTAC 3 cut(s) 253, 674, 900
RseI CAYNNNNRTG 1 cut(s) 382
SacI GAGCTC 1 cut(s) 738
SacII CCGCGG 1 cut(s) 160
SalI GTCGAC 2 cut(s) 131, 1073
SapI GCTCTTC 1 cut(s) 1094
SaqAI TTAA 2 cut(s) 488, 527
SatI GCNGC 5 cut(s) 27, 157, 160, 375, 503
Sau3AI GATC 4 cut(s) 106, 168, 463, 904
Sau96I GGNCC 1 cut(s) 452
ScaI AGTACT 1 cut(s) 901
SchI GAGTC 1 cut(s) 540
ScrFI CCNGG 2 cut(s) 1063, 1131
SduI GDGCHC 1 cut(s) 738
Sfr303I CCGCGG 1 cut(s) 160
SgrBI CCGCGG 1 cut(s) 160
SgrDI CGTCGACG 1 cut(s) 131
SmiMI CAYNNNNRTG 1 cut(s) 382
Sse9I AATT 4 cut(s) 269, 495, 727, 1122
SsiI CCGC 4 cut(s) 110, 157, 159, 714
SspI AATATT 1 cut(s) 955
SspMI CTAG 2 cut(s) 861, 1085
SstI GAGCTC 1 cut(s) 738
StyD4I CCNGG 2 cut(s) 1061, 1129
StyI CCWWGG 2 cut(s) 21, 860
TaaI ACNGT 7 cut(s) 122, 460, 725, 781, 848, 1049, 1079
TaiI ACGT 5 cut(s) 132, 138, 206, 423, 802
TaqI TCGA 8 cut(s) 132, 267, 484, 783, 873, 981, 1074, 1126
TasI AATT 4 cut(s) 269, 495, 727, 1122
TatI WGTACW 1 cut(s) 899
TauI GCSGC 2 cut(s) 159, 162
TfiI GAWTC 1 cut(s) 1002
Tru1I TTAA 2 cut(s) 488, 527
Tru9I TTAA 2 cut(s) 488, 527
TscAI CASTG 3 cut(s) 600, 728, 937
TseFI GTSAC 1 cut(s) 1026
TseI GCWGC 3 cut(s) 26, 374, 502
Tsp45I GTSAC 1 cut(s) 1026
TspDTI ATGAA 4 cut(s) 6, 651, 708, 760
TspGWI ACGGA 3 cut(s) 464, 639, 1111
TspRI CASTG 3 cut(s) 600, 728, 937
Van91I CCANNNNNTGG 1 cut(s) 196
XapI RAATTY 1 cut(s) 1122
XmaJI CCTAGG 1 cut(s) 860
XmiI GTMKAC 3 cut(s) 132, 990, 1074
XspI CTAG 2 cut(s) 861, 1085
ZrmI AGTACT 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.