Prupe.5G160600_v2.0.a1

domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0023575
Physical Location & Seq
Forward (+)
14157971 .. 14161024
3054 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G160600.1

Sequence Viewer

Length: 1137 bp
ATGGGTGGGGTTACTTCATCCATGGCTGCAAAGTTTGCCTTTTTCCCACCCAACCCACCATCCTACAAGGTAGTTAAAGACGAAGCCACTGGTCTCTTGCTCATGGACCCTCTTCCTCATCGTGAAAACGTCGACGTTTTGAAGTTTCCCACTCGCCGCGGCAATGAGATCGTGGCCGTTTATATACGCCACCCAATGGCCACCTCTACCCTGCTGTACTCTCATGGCAACGCCACTGATATCGGTCAAATGTACGAGCTTTTCATCGAATTGAGCATTCACCTCCGCGTCAATCTCATGGGGTATGATTACTCTGGCTATGGACAGTCATCAGGCAAGCCAAGTGAACATAATACTTATGCAGATATTGAAGCTGCATATAAGTGTCTTGAAGAGAGGTATGGGGCTAAGCAGGAAGACATAATCCTCTATGGTCAGTCTGTTGGAAGCGGTCCTACTGTTGATCTTGCTGCCCGTTTGCCTCGACTGAGAGCAATTGTTTTGCATAGCCCTATATTATCAGGTTTAAGAGTCATGTATCCTGTGAAACGCACGTACTGGTTTGATATCTATAAGAACATTGATAAAATCCCTCTGGTGAAATGTCCTGTGCTGGTAATACATGGAACAGCTGATGAAGTTGTCGACTGCTCACACGGCAAGCAACTTTGGGTACTGTGTCAAGAGAAATATGAACCTCTATGGCTAAAAGGCGGAAGTCACTGTAATTTGGAACTGTATCCAGAATATCTTAGACATCTCAATAAGTTCATATCAACTGTTGAAAAATCAGCTTCGCGAAGGATTAGTTCTAGAAAAAGCACAGACCATAGGAAAAGCACGGACCGCATCGAACTTCCTAGGAGGAGTGTTGATTTTTTTGAGGCTCCAAGGAAGAGTACTGATCGGAGAGAGAAATCAAGGAAGAGCACTGATAGACCCGAAAAGCTGAAATTTAGTGAATACAAGTTTAATAACGTTGACAAGGTAGATAAGTTTAGGATCTCTGCTGACCAGATAGAGAGGTCACGAAGAAGCGTGGAATACCATCACGAGAAATCTAGGAGAAGCATTGACCATCAGCTAGAAAAAGCGCGGAAGAGCGTTGACTGGCTGGACAGAATTCGAGCTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

379

Amino Acids

43.58

Weight (kDa)

9.39

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023575)

Species Orthologous Gene IDs
malus_domestica MD14G1165900.v1.1
prunus_persica Prupe.5G160600_v2.0.a1
pyrus_communis pycom14g13850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 196
AccI GTMKAC 2 cut(s) 132, 645
AccII CGCG 4 cut(s) 159, 288, 799, 1096
AciI CCGC 7 cut(s) 157, 159, 286, 450, 714, 847, 1096
AclI AACGTT 1 cut(s) 978
AclWI GGATC 1 cut(s) 1010
AcoI YGGCCR 2 cut(s) 174, 198
AcsI RAATTY 2 cut(s) 953, 1122
AfaI GTAC 5 cut(s) 218, 254, 557, 675, 901
AfiI CCNNNNNNNGG 1 cut(s) 196
AgsI TTSAA 4 cut(s) 142, 371, 392, 785
AluBI AGCT 7 cut(s) 259, 374, 632, 794, 949, 1084, 1130
AluI AGCT 7 cut(s) 259, 374, 632, 794, 949, 1084, 1130
Alw21I GWGCWC 1 cut(s) 932
Alw26I GTCTC 1 cut(s) 98
AlwI GGATC 1 cut(s) 1010
AoxI GGCC 2 cut(s) 174, 198
ApeKI GCWGC 3 cut(s) 26, 374, 470
ApoI RAATTY 2 cut(s) 953, 1122
AspA2I CCTAGG 1 cut(s) 860
AspLEI GCGC 1 cut(s) 1096
AspS9I GGNCC 3 cut(s) 106, 452, 844
AsuHPI GGTGA 2 cut(s) 272, 610
AvaII GGWCC 3 cut(s) 106, 452, 844
AvrII CCTAGG 1 cut(s) 860
BalI TGGCCA 1 cut(s) 200
BarI GAAGNNNNNNTAC 2 cut(s) 439, 471
BauI CACGAG 1 cut(s) 1052
BbsI GAAGAC 1 cut(s) 423
Bbv12I GWGCWC 1 cut(s) 932
BbvI GCAGC 3 cut(s) 13, 361, 457
BccI CCATC 3 cut(s) 67, 1056, 1086
BceAI ACGGC 2 cut(s) 161, 673
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BciVI GTATCC 2 cut(s) 549, 750
BcoDI GTCTC 1 cut(s) 98
BfaI CTAG 4 cut(s) 813, 861, 1062, 1085
BfuI GTATCC 2 cut(s) 549, 750
BisI GCNGC 5 cut(s) 27, 157, 160, 375, 471
BlnI CCTAGG 1 cut(s) 860
BlpI GCTNAGC 1 cut(s) 408
BlsI GCNGC 5 cut(s) 28, 158, 161, 376, 472
BmcAI AGTACT 1 cut(s) 901
Bme18I GGWCC 3 cut(s) 106, 452, 844
BmgT120I GGNCC 3 cut(s) 106, 452, 844
BmiI GGNNCC 2 cut(s) 108, 888
BmsI GCATC 1 cut(s) 858
BpiI GAAGAC 1 cut(s) 423
Bpu1102I GCTNAGC 1 cut(s) 408
BsaAI YACGTR 1 cut(s) 555
BsaI GGTCTC 1 cut(s) 98
BsaJI CCNNGG 4 cut(s) 21, 157, 860, 890
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse1I ACTGG 3 cut(s) 94, 563, 1115
Bse3DI GCAATG 1 cut(s) 169
BseDI CCNNGG 4 cut(s) 21, 157, 860, 890
BseGI GGATG 2 cut(s) 17, 59
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 1 cut(s) 479
BseNI ACTGG 3 cut(s) 94, 563, 1115
BseRI GAGGAG 1 cut(s) 880
BseXI GCAGC 3 cut(s) 13, 361, 457
BseYI CCCAGC 1 cut(s) 1130
Bsh1236I CGCG 4 cut(s) 159, 288, 799, 1096
BshFI GGCC 2 cut(s) 176, 200
BsiHKAI GWGCWC 1 cut(s) 932
BslI CCNNNNNNNGG 1 cut(s) 196
BsmAI GTCTC 1 cut(s) 98
BsmI GAATGC 1 cut(s) 276
BsnI GGCC 2 cut(s) 176, 200
Bso31I GGTCTC 1 cut(s) 98
Bsp1286I GDGCHC 1 cut(s) 932
Bsp143I GATC 4 cut(s) 168, 463, 904, 1002
Bsp1720I GCTNAGC 1 cut(s) 408
Bsp19I CCATGG 1 cut(s) 21
Bsp68I TCGCGA 1 cut(s) 799
BspACI CCGC 7 cut(s) 157, 159, 286, 450, 714, 847, 1096
BspANI GGCC 2 cut(s) 176, 200
BspCNI CTCAG 1 cut(s) 480
BspFNI CGCG 4 cut(s) 159, 288, 799, 1096
BspLI GGNNCC 2 cut(s) 108, 888
BspPI GGATC 1 cut(s) 1010
BspQI GCTCTTC 2 cut(s) 920, 1094
BspTNI GGTCTC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 169
BsrI ACTGG 3 cut(s) 94, 563, 1115
BssECI CCNNGG 4 cut(s) 21, 157, 860, 890
BssMI GATC 4 cut(s) 168, 463, 904, 1002
BssSI CACGAG 1 cut(s) 1052
BssT1I CCWWGG 3 cut(s) 21, 860, 890
Bst2BI CACGAG 1 cut(s) 1052
Bst4CI ACNGT 6 cut(s) 327, 460, 678, 725, 738, 781
Bst6I CTCTTC 5 cut(s) 117, 387, 890, 920, 1094
BstAPI GCANNNNNTGC 1 cut(s) 35
BstBAI YACGTR 1 cut(s) 555
BstC8I GCNNGC 2 cut(s) 338, 662
BstDEI CTNAG 3 cut(s) 408, 488, 752
BstDSI CCRYGG 2 cut(s) 21, 157
BstF5I GGATG 2 cut(s) 17, 59
BstFNI CGCG 4 cut(s) 159, 288, 799, 1096
BstHHI GCGC 1 cut(s) 1096
BstKTI GATC 4 cut(s) 171, 466, 907, 1005
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 4 cut(s) 168, 463, 904, 1002
BstMWI GCNNNNNNNGC 3 cut(s) 35, 657, 846
BstUI CGCG 4 cut(s) 159, 288, 799, 1096
BstV1I GCAGC 3 cut(s) 13, 361, 457
BstV2I GAAGAC 1 cut(s) 423
BstX2I RGATCY 1 cut(s) 1002
BstYI RGATCY 1 cut(s) 1002
BsuI GTATCC 2 cut(s) 549, 750
BsuRI GGCC 2 cut(s) 176, 200
BtgI CCRYGG 2 cut(s) 21, 157
BtsCI GGATG 2 cut(s) 17, 59
BtsIMutI CAGTG 4 cut(s) 87, 234, 721, 930
BtuMI TCGCGA 1 cut(s) 799
Cac8I GCNNGC 2 cut(s) 338, 662
CfoI GCGC 1 cut(s) 1096
Cfr13I GGNCC 3 cut(s) 106, 452, 844
Cfr42I CCGCGG 1 cut(s) 160
CpoI CGGWCCG 1 cut(s) 844
CseI GACGC 1 cut(s) 277
Csp6I GTAC 5 cut(s) 217, 253, 556, 674, 900
CspI CGGWCCG 1 cut(s) 844
CviAII CATG 6 cut(s) 22, 103, 224, 298, 535, 623
CviQI GTAC 5 cut(s) 217, 253, 556, 674, 900
DdeI CTNAG 3 cut(s) 408, 488, 752
DpnI GATC 4 cut(s) 170, 465, 906, 1004
DpnII GATC 4 cut(s) 168, 463, 904, 1002
EaeI YGGCCR 2 cut(s) 174, 198
Eam1104I CTCTTC 5 cut(s) 117, 387, 890, 920, 1094
EarI CTCTTC 5 cut(s) 117, 387, 890, 920, 1094
EciI GGCGGA 1 cut(s) 729
Eco130I CCWWGG 3 cut(s) 21, 860, 890
Eco31I GGTCTC 1 cut(s) 98
Eco32I GATATC 2 cut(s) 241, 568
Eco47I GGWCC 3 cut(s) 106, 452, 844
EcoRI GAATTC 1 cut(s) 1122
EcoRV GATATC 2 cut(s) 241, 568
EcoT14I CCWWGG 3 cut(s) 21, 860, 890
ErhI CCWWGG 3 cut(s) 21, 860, 890
FaeI CATG 6 cut(s) 25, 106, 227, 301, 538, 626
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FatI CATG 6 cut(s) 21, 102, 223, 297, 534, 622
FblI GTMKAC 2 cut(s) 132, 645
Fnu4HI GCNGC 5 cut(s) 27, 157, 160, 375, 471
FokI GGATG 2 cut(s) 4, 46
Fsp4HI GCNGC 5 cut(s) 27, 157, 160, 375, 471
FspBI CTAG 4 cut(s) 813, 861, 1062, 1085
GlaI GCGC 1 cut(s) 1095
GluI GCNGC 5 cut(s) 27, 157, 160, 375, 471
GsaI CCCAGC 1 cut(s) 1134
HaeIII GGCC 2 cut(s) 176, 200
HgaI GACGC 1 cut(s) 277
HhaI GCGC 1 cut(s) 1096
Hin1II CATG 6 cut(s) 25, 106, 227, 301, 538, 626
Hin6I GCGC 1 cut(s) 1094
HinP1I GCGC 1 cut(s) 1094
HincII GTYRAC 4 cut(s) 133, 646, 982, 1108
HindII GTYRAC 4 cut(s) 133, 646, 982, 1108
HinfI GANTC 1 cut(s) 531
HphI GGTGA 2 cut(s) 272, 610
Hpy166II GTNNAC 5 cut(s) 133, 347, 646, 982, 1108
Hpy188I TCNGA 1 cut(s) 909
Hpy188III TCNNGA 8 cut(s) 122, 389, 683, 743, 798, 813, 1029, 1052
Hpy8I GTNNAC 5 cut(s) 133, 347, 646, 982, 1108
Hpy99I CGWCG 2 cut(s) 134, 137
HpyAV CCTTC 1 cut(s) 795
HpyCH4III ACNGT 6 cut(s) 327, 460, 678, 725, 738, 781
HpyCH4IV ACGT 4 cut(s) 129, 135, 554, 978
HpyCH4V TGCA 4 cut(s) 29, 362, 377, 505
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 657, 846
HpyF3I CTNAG 3 cut(s) 408, 488, 752
HpySE526I ACGT 4 cut(s) 129, 135, 554, 978
Hsp92II CATG 6 cut(s) 25, 106, 227, 301, 538, 626
HspAI GCGC 1 cut(s) 1094
KspI CCGCGG 1 cut(s) 160
Kzo9I GATC 4 cut(s) 168, 463, 904, 1002
LguI GCTCTTC 2 cut(s) 920, 1094
LmnI GCTCC 1 cut(s) 892
Lsp1109I GCAGC 3 cut(s) 13, 361, 457
LweI GCATC 1 cut(s) 858
MaeI CTAG 4 cut(s) 813, 861, 1062, 1085
MaeII ACGT 4 cut(s) 129, 135, 554, 978
MaeIII GTNAC 3 cut(s) 10, 719, 1026
MalI GATC 4 cut(s) 170, 465, 906, 1004
MboI GATC 4 cut(s) 168, 463, 904, 1002
MboII GAAGA 7 cut(s) 104, 404, 428, 907, 937, 1044, 1111
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 1 cut(s) 1002
MhlI GDGCHC 1 cut(s) 932
MlsI TGGCCA 1 cut(s) 200
MluCI AATT 5 cut(s) 269, 495, 727, 953, 1122
MluNI TGGCCA 1 cut(s) 200
MlyI GAGTC 1 cut(s) 540
MmeI TCCRAC 1 cut(s) 424
Mox20I TGGCCA 1 cut(s) 200
MscI TGGCCA 1 cut(s) 200
MseI TTAA 3 cut(s) 75, 527, 972
MslI CAYNNNNRTG 1 cut(s) 382
Msp20I TGGCCA 1 cut(s) 200
MspA1I CMGCKG 2 cut(s) 159, 632
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 276
MvnI CGCG 4 cut(s) 159, 288, 799, 1096
MwoI GCNNNNNNNGC 3 cut(s) 35, 657, 846
NcoI CCATGG 1 cut(s) 21
NdeII GATC 4 cut(s) 168, 463, 904, 1002
NlaIII CATG 6 cut(s) 25, 106, 227, 301, 538, 626
NlaIV GGNNCC 2 cut(s) 108, 888
NmuCI GTSAC 2 cut(s) 719, 1026
NruI TCGCGA 1 cut(s) 799
PciSI GCTCTTC 2 cut(s) 920, 1094
PctI GAATGC 1 cut(s) 276
PflMI CCANNNNNTGG 1 cut(s) 196
PkrI GCNGC 5 cut(s) 28, 158, 161, 376, 472
PleI GAGTC 1 cut(s) 539
PpsI GAGTC 1 cut(s) 539
Ppu21I YACGTR 1 cut(s) 555
Psp1406I AACGTT 1 cut(s) 978
PspFI CCCAGC 1 cut(s) 1130
PspN4I GGNNCC 2 cut(s) 108, 888
PspPI GGNCC 3 cut(s) 106, 452, 844
PsuI RGATCY 1 cut(s) 1002
PvuII CAGCTG 1 cut(s) 632
RruI TCGCGA 1 cut(s) 799
RsaI GTAC 5 cut(s) 218, 254, 557, 675, 901
RsaNI GTAC 5 cut(s) 217, 253, 556, 674, 900
RseI CAYNNNNRTG 1 cut(s) 382
Rsr2I CGGWCCG 1 cut(s) 844
RsrII CGGWCCG 1 cut(s) 844
SacII CCGCGG 1 cut(s) 160
SalI GTCGAC 2 cut(s) 131, 644
SapI GCTCTTC 2 cut(s) 920, 1094
SaqAI TTAA 3 cut(s) 75, 527, 972
SatI GCNGC 5 cut(s) 27, 157, 160, 375, 471
Sau3AI GATC 4 cut(s) 168, 463, 904, 1002
Sau96I GGNCC 3 cut(s) 106, 452, 844
ScaI AGTACT 1 cut(s) 901
SchI GAGTC 1 cut(s) 540
SduI GDGCHC 1 cut(s) 932
SfaNI GCATC 1 cut(s) 858
Sfr303I CCGCGG 1 cut(s) 160
SgrBI CCGCGG 1 cut(s) 160
SgrDI CGTCGACG 1 cut(s) 131
SinI GGWCC 3 cut(s) 106, 452, 844
SmiMI CAYNNNNRTG 1 cut(s) 382
Sse9I AATT 5 cut(s) 269, 495, 727, 953, 1122
SsiI CCGC 7 cut(s) 157, 159, 286, 450, 714, 847, 1096
SspMI CTAG 4 cut(s) 813, 861, 1062, 1085
StyI CCWWGG 3 cut(s) 21, 860, 890
TaaI ACNGT 6 cut(s) 327, 460, 678, 725, 738, 781
TaiI ACGT 4 cut(s) 132, 138, 557, 981
TaqI TCGA 6 cut(s) 132, 267, 484, 645, 852, 1126
TaqII GACCGA 1 cut(s) 233
TasI AATT 5 cut(s) 269, 495, 727, 953, 1122
TatI WGTACW 2 cut(s) 216, 899
TauI GCSGC 2 cut(s) 159, 162
Tru1I TTAA 3 cut(s) 75, 527, 972
Tru9I TTAA 3 cut(s) 75, 527, 972
TscAI CASTG 4 cut(s) 94, 241, 728, 937
TseFI GTSAC 2 cut(s) 719, 1026
TseI GCWGC 3 cut(s) 26, 374, 470
Tsp45I GTSAC 2 cut(s) 719, 1026
TspDTI ATGAA 5 cut(s) 6, 253, 651, 708, 760
TspGWI ACGGA 1 cut(s) 857
TspRI CASTG 4 cut(s) 94, 241, 728, 937
Van91I CCANNNNNTGG 1 cut(s) 196
VpaK11BI GGWCC 3 cut(s) 106, 452, 844
XapI RAATTY 2 cut(s) 953, 1122
XbaI TCTAGA 1 cut(s) 812
XmaJI CCTAGG 1 cut(s) 860
XmiI GTMKAC 2 cut(s) 132, 645
XspI CTAG 4 cut(s) 813, 861, 1062, 1085
ZrmI AGTACT 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.