Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0024264

Physical Location & Seq

Genomic Coordinates Forward (+)

29219594 .. 29221963

Gene Structure 2370 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1204000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 924 bp

ATGGCCAGTCGGAGCGAGGACCGTTGCGAAATAGCCTTCTTCGACTTGGAGACGACGATGCCGACCCGAACCGACCAGGACCTCACCATCTTGGAATTCGGGTCGATTCTCGTTTGCCCCAAGAAGCTGGTGGAGCTCGACAACTACTCCACCCTGGTCCGACCCGCCGACCCCTCCGTCATCAATTCCCTGCCCGACCGCCGCAACGGCATTACCCGCGCCGCCCTCATCGACGCCCCTTTTTTTCAAGACATCGCCGACAAGGTCTACGACATTCTCCACGGACGTATATGGGCTGGTCACAACATTCTCCAGTTTGATTGTGTGCTGATTCGGCAGGCTTTCGCGCAGATTGGTCGGCCTGCACCAGAACCGAAAGATACAATCGATTCATTGGAATTCTTGAAACAGCAGTTCGGAACGAAAGCTGGTAACATGAAGATGGCCTCCCTTGCAACCTATTTCGGGCTTGGACAGCAGACACACAGGAGTTTGGATGATGTTCGAATGAATCTGGAAGTTCTGAAATGCTGTGCGGCCGTTTTATTCTTGGAGTCGAGCCTCCCGAACATATTCAAGAAGAATAGTTGGGAGTTTTTACAGCCAGATGAAGTTTCTATTCCTTCCATCCGTCCTACCCTTTTGATGTTGGATAATGGGAGTCTTAGGATAAAGTTGTTACACAAAGATGAAACTTTGCAGCTTTGCTGTAGGCATATGGAATTGCTGTTTGGAATCAATGAAGAGTATTTTGATCTAACTGGCCGATTGGATATTGTTTGTTTCGCATCACAAAATTTATGCGAAGTTCTTGATGCATGTGATGGCGTTGCACAAAAGTTGTCTATGGATTCTGGTAGCAGCTCCGAATGGCAGCCTGCTGTGATCCGAAGGGAGGGCTTTTATACAGTGAGATTACAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006139 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008408 GO:0009380 GO:0009987 GO:0016020 GO:0016787 GO:0016788 GO:0030312 GO:0032991 GO:0033554 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0071944 GO:0090304 GO:0090305 GO:1901360 GO:1902494 GO:1905347 GO:1905348 GO:1990391

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RNase_T

Protein Analysis

308

Amino Acids

34.7

Weight (kDa)

5.07

Isoelectric Point (pI)

51.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNase_T	PF00929	13 - 175	2.4e-24	Exonuclease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0024264)

Species	Orthologous Gene IDs
malus_domestica	MD14G1204000.v1.1 MD14G1204100.v1.1
prunus_persica	Prupe.5G197600_v2.0.a1

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	176
AccI	GTMKAC	1 cut(s)	267
AccII	CGCG	2 cut(s)	219, 347
AciI	CCGC	6 cut(s)	165, 199, 202, 217, 222, 536
AclWI	GGATC	1 cut(s)	880
AcoI	YGGCCR	3 cut(s)	3, 537, 763
AcsI	RAATTY	3 cut(s)	95, 398, 796
AcyI	GRCGYC	1 cut(s)	234
AfiI	CCNNNNNNNGG	2 cut(s)	465, 895
AgsI	TTSAA	3 cut(s)	248, 406, 577
AjnI	CCWGG	2 cut(s)	75, 153
AjuI	GAANNNNNNNTTGG	2 cut(s)	714, 746
AluBI	AGCT	5 cut(s)	127, 136, 428, 703, 864
AluI	AGCT	5 cut(s)	127, 136, 428, 703, 864
Alw21I	GWGCWC	1 cut(s)	138
Alw26I	GTCTC	1 cut(s)	44
AlwI	GGATC	1 cut(s)	880
AoxI	GGCC	5 cut(s)	3, 359, 444, 537, 763
ApeKI	GCWGC	3 cut(s)	700, 861, 874
ApoI	RAATTY	3 cut(s)	95, 398, 796
Asp700I	GAANNNNTTC	1 cut(s)	572
AspLEI	GCGC	2 cut(s)	221, 349
AspS9I	GGNCC	3 cut(s)	19, 79, 157
AsuHPI	GGTGA	1 cut(s)	76
AsuII	TTCGAA	1 cut(s)	505
AvaII	GGWCC	3 cut(s)	19, 79, 157
BalI	TGGCCA	1 cut(s)	5
BanII	GRGCYC	1 cut(s)	138
Bbv12I	GWGCWC	1 cut(s)	138
BbvI	GCAGC	3 cut(s)	712, 873, 886
BccI	CCATC	4 cut(s)	95, 436, 635, 818
BceAI	ACGGC	2 cut(s)	223, 524
BcgI	CGANNNNNNTGC	2 cut(s)	338, 372
BciT130I	CCWGG	2 cut(s)	77, 155
BcoDI	GTCTC	1 cut(s)	44
BfmI	CTRYAG	1 cut(s)	709
BglI	GCCNNNNNGGC	1 cut(s)	207
BisI	GCNGC	6 cut(s)	202, 222, 537, 701, 862, 875
BlsI	GCNGC	6 cut(s)	203, 223, 538, 702, 863, 876
Bme1390I	CCNGG	2 cut(s)	77, 155
Bme18I	GGWCC	3 cut(s)	19, 79, 157
BmgT120I	GGNCC	3 cut(s)	19, 79, 157
BmrFI	CCNGG	2 cut(s)	77, 155
BmsI	GCATC	3 cut(s)	48, 797, 805
BpmI	CTGGAG	1 cut(s)	296
Bpu14I	TTCGAA	1 cut(s)	505
Bsa29I	ATCGAT	1 cut(s)	387
BsaHI	GRCGYC	1 cut(s)	234
BsaJI	CCNNGG	2 cut(s)	153, 280
BsaXI	ACNNNNNCTCC	2 cut(s)	131, 161
Bsc4I	CCNNNNNNNGG	2 cut(s)	465, 895
Bse1I	ACTGG	3 cut(s)	6, 313, 766
BseBI	CCWGG	2 cut(s)	77, 155
BseCI	ATCGAT	1 cut(s)	387
BseDI	CCNNGG	2 cut(s)	153, 280
BseGI	GGATG	2 cut(s)	502, 627
BseLI	CCNNNNNNNGG	2 cut(s)	465, 895
BseNI	ACTGG	3 cut(s)	6, 313, 766
BseX3I	CGGCCG	1 cut(s)	537
BseXI	GCAGC	3 cut(s)	712, 873, 886
BsgI	GTGCAG	1 cut(s)	348
Bsh1236I	CGCG	2 cut(s)	219, 347
Bsh1285I	CGRYCG	2 cut(s)	199, 540
BshFI	GGCC	5 cut(s)	5, 361, 446, 539, 765
BshVI	ATCGAT	1 cut(s)	387
BsiEI	CGRYCG	2 cut(s)	199, 540
BsiHKAI	GWGCWC	1 cut(s)	138
BslI	CCNNNNNNNGG	2 cut(s)	465, 895
BsmAI	GTCTC	1 cut(s)	44
BsmBI	CGTCTC	1 cut(s)	44
BsnI	GGCC	5 cut(s)	5, 361, 446, 539, 765
Bsp119I	TTCGAA	1 cut(s)	505
Bsp1286I	GDGCHC	1 cut(s)	138
Bsp143I	GATC	2 cut(s)	754, 885
BspACI	CCGC	6 cut(s)	165, 199, 202, 217, 222, 536
BspANI	GGCC	5 cut(s)	5, 361, 446, 539, 765
BspDI	ATCGAT	1 cut(s)	387
BspFNI	CGCG	2 cut(s)	219, 347
BspPI	GGATC	1 cut(s)	880
BspT104I	TTCGAA	1 cut(s)	505
BsrI	ACTGG	3 cut(s)	6, 313, 766
BssECI	CCNNGG	2 cut(s)	153, 280
BssMI	GATC	2 cut(s)	754, 885
BssNI	GRCGYC	1 cut(s)	234
Bst2UI	CCWGG	2 cut(s)	77, 155
Bst4CI	ACNGT	3 cut(s)	23, 910, 921
Bst6I	CTCTTC	1 cut(s)	738
BstACI	GRCGYC	1 cut(s)	234
BstBI	TTCGAA	1 cut(s)	505
BstC8I	GCNNGC	3 cut(s)	339, 363, 879
BstDEI	CTNAG	1 cut(s)	665
BstDSI	CCRYGG	1 cut(s)	280
BstF5I	GGATG	2 cut(s)	502, 627
BstFNI	CGCG	2 cut(s)	219, 347
BstHHI	GCGC	2 cut(s)	221, 349
BstKTI	GATC	2 cut(s)	757, 888
BstMAI	GTCTC	1 cut(s)	44
BstMBI	GATC	2 cut(s)	754, 885
BstMCI	CGRYCG	2 cut(s)	199, 540
BstMWI	GCNNNNNNNGC	6 cut(s)	133, 207, 216, 334, 452, 475
BstNI	CCWGG	2 cut(s)	77, 155
BstNSI	RCATGY	1 cut(s)	822
BstSCI	CCNGG	2 cut(s)	75, 153
BstSFI	CTRYAG	1 cut(s)	709
BstUI	CGCG	2 cut(s)	219, 347
BstV1I	GCAGC	3 cut(s)	712, 873, 886
BstXI	CCANNNNNNTGG	1 cut(s)	127
BstZI	CGGCCG	1 cut(s)	537
Bsu15I	ATCGAT	1 cut(s)	387
BsuRI	GGCC	5 cut(s)	5, 361, 446, 539, 765
BsuTUI	ATCGAT	1 cut(s)	387
BtgI	CCRYGG	1 cut(s)	280
BtgZI	GCGATG	1 cut(s)	238
BtsCI	GGATG	2 cut(s)	502, 627
BtsIMutI	CAGTG	1 cut(s)	915
Cac8I	GCNNGC	3 cut(s)	339, 363, 879
CfoI	GCGC	2 cut(s)	221, 349
Cfr13I	GGNCC	3 cut(s)	19, 79, 157
ClaI	ATCGAT	1 cut(s)	387
CseI	GACGC	1 cut(s)	242
CviAII	CATG	2 cut(s)	436, 819
DdeI	CTNAG	1 cut(s)	665
DpnI	GATC	2 cut(s)	756, 887
DpnII	GATC	2 cut(s)	754, 885
DrdI	GACNNNNNNGTC	1 cut(s)	176
DseDI	GACNNNNNNGTC	1 cut(s)	176
EaeI	YGGCCR	3 cut(s)	3, 537, 763
EagI	CGGCCG	1 cut(s)	537
Eam1104I	CTCTTC	1 cut(s)	738
EarI	CTCTTC	1 cut(s)	738
Ecl136II	GAGCTC	1 cut(s)	136
EclXI	CGGCCG	1 cut(s)	537
Eco24I	GRGCYC	1 cut(s)	138
Eco47I	GGWCC	3 cut(s)	19, 79, 157
Eco52I	CGGCCG	1 cut(s)	537
Eco53kI	GAGCTC	1 cut(s)	136
EcoICRI	GAGCTC	1 cut(s)	136
EcoO109I	RGGNCCY	1 cut(s)	79
EcoRI	GAATTC	2 cut(s)	95, 398
EcoRII	CCWGG	2 cut(s)	75, 153
EcoT22I	ATGCAT	1 cut(s)	820
EcoT38I	GRGCYC	1 cut(s)	138
Esp3I	CGTCTC	1 cut(s)	44
FaeI	CATG	2 cut(s)	439, 822
FatI	CATG	2 cut(s)	435, 818
FauI	CCCGC	2 cut(s)	172, 224
FauNDI	CATATG	1 cut(s)	717
FblI	GTMKAC	1 cut(s)	267
Fnu4HI	GCNGC	6 cut(s)	202, 222, 537, 701, 862, 875
FokI	GGATG	2 cut(s)	509, 614
FriOI	GRGCYC	1 cut(s)	138
Fsp4HI	GCNGC	6 cut(s)	202, 222, 537, 701, 862, 875
GlaI	GCGC	2 cut(s)	220, 348
GluI	GCNGC	6 cut(s)	202, 222, 537, 701, 862, 875
GsuI	CTGGAG	1 cut(s)	296
HaeIII	GGCC	5 cut(s)	5, 361, 446, 539, 765
HgaI	GACGC	1 cut(s)	242
HhaI	GCGC	2 cut(s)	221, 349
Hin1I	GRCGYC	1 cut(s)	234
Hin1II	CATG	2 cut(s)	439, 822
Hin6I	GCGC	2 cut(s)	219, 347
HinP1I	GCGC	2 cut(s)	219, 347
HinfI	GANTC	8 cut(s)	106, 331, 389, 511, 554, 661, 735, 851
HphI	GGTGA	1 cut(s)	76
Hpy166II	GTNNAC	1 cut(s)	268
Hpy188I	TCNGA	6 cut(s)	12, 161, 419, 525, 868, 890
Hpy188III	TCNNGA	6 cut(s)	248, 403, 515, 565, 577, 812
Hpy8I	GTNNAC	1 cut(s)	268
Hpy99I	CGWCG	2 cut(s)	58, 236
HpyAV	CCTTC	3 cut(s)	46, 633, 885
HpyCH4III	ACNGT	3 cut(s)	23, 910, 921
HpyCH4IV	ACGT	1 cut(s)	286
HpyCH4V	TGCA	5 cut(s)	365, 455, 700, 818, 833
HpyF10VI	GCNNNNNNNGC	6 cut(s)	133, 207, 216, 334, 452, 475
HpyF3I	CTNAG	1 cut(s)	665
HpySE526I	ACGT	1 cut(s)	286
Hsp92I	GRCGYC	1 cut(s)	234
Hsp92II	CATG	2 cut(s)	439, 822
HspAI	GCGC	2 cut(s)	219, 347
Kzo9I	GATC	2 cut(s)	754, 885
LmnI	GCTCC	3 cut(s)	12, 133, 869
Lsp1109I	GCAGC	3 cut(s)	712, 873, 886
LweI	GCATC	3 cut(s)	48, 797, 805
MaeII	ACGT	1 cut(s)	286
MaeIII	GTNAC	3 cut(s)	299, 431, 678
MalI	GATC	2 cut(s)	756, 887
MboI	GATC	2 cut(s)	754, 885
MboII	GAAGA	4 cut(s)	31, 451, 592, 755
MhlI	GDGCHC	1 cut(s)	138
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	5 cut(s)	95, 184, 398, 722, 796
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	2 cut(s)	563, 670
MmeI	TCCRAC	2 cut(s)	184, 630
MnlI	CCTC	7 cut(s)	10, 92, 184, 236, 457, 572, 889
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	820
MroXI	GAANNNNTTC	1 cut(s)	572
MscI	TGGCCA	1 cut(s)	5
MslI	CAYNNNNRTG	2 cut(s)	440, 687
Msp20I	TGGCCA	1 cut(s)	5
MspR9I	CCNGG	2 cut(s)	77, 155
MvaI	CCWGG	2 cut(s)	77, 155
MvnI	CGCG	2 cut(s)	219, 347
MwoI	GCNNNNNNNGC	6 cut(s)	133, 207, 216, 334, 452, 475
NdeI	CATATG	1 cut(s)	717
NdeII	GATC	2 cut(s)	754, 885
NlaIII	CATG	2 cut(s)	439, 822
NmuCI	GTSAC	1 cut(s)	299
NsiI	ATGCAT	1 cut(s)	820
NspI	RCATGY	1 cut(s)	822
NspV	TTCGAA	1 cut(s)	505
PcsI	WCGNNNNNNNCGW	2 cut(s)	59, 563
PdmI	GAANNNNTTC	1 cut(s)	572
PfeI	GAWTC	6 cut(s)	106, 331, 389, 511, 735, 851
PflFI	GACNNNGTC	1 cut(s)	263
PkrI	GCNGC	6 cut(s)	203, 223, 538, 702, 863, 876
PleI	GAGTC	2 cut(s)	562, 669
PpsI	GAGTC	2 cut(s)	562, 669
PpuMI	RGGWCCY	1 cut(s)	79
Psp124BI	GAGCTC	1 cut(s)	138
Psp5II	RGGWCCY	1 cut(s)	79
Psp6I	CCWGG	2 cut(s)	75, 153
PspGI	CCWGG	2 cut(s)	75, 153
PspPI	GGNCC	3 cut(s)	19, 79, 157
PspPPI	RGGWCCY	1 cut(s)	79
PsyI	GACNNNGTC	1 cut(s)	263
RseI	CAYNNNNRTG	2 cut(s)	440, 687
SacI	GAGCTC	1 cut(s)	138
SatI	GCNGC	6 cut(s)	202, 222, 537, 701, 862, 875
Sau3AI	GATC	2 cut(s)	754, 885
Sau96I	GGNCC	3 cut(s)	19, 79, 157
SchI	GAGTC	2 cut(s)	563, 670
ScrFI	CCNGG	2 cut(s)	77, 155
SduI	GDGCHC	1 cut(s)	138
SetI	ASST	9 cut(s)	84, 129, 138, 267, 289, 430, 461, 705, 866
SfaNI	GCATC	3 cut(s)	48, 797, 805
SfcI	CTRYAG	1 cut(s)	709
SfuI	TTCGAA	1 cut(s)	505
SinI	GGWCC	3 cut(s)	19, 79, 157
SmiMI	CAYNNNNRTG	2 cut(s)	440, 687
Sse9I	AATT	5 cut(s)	95, 184, 398, 722, 796
SsiI	CCGC	6 cut(s)	165, 199, 202, 217, 222, 536
SstI	GAGCTC	1 cut(s)	138
StyD4I	CCNGG	2 cut(s)	75, 153
TaaI	ACNGT	3 cut(s)	23, 910, 921
TaiI	ACGT	1 cut(s)	289
TaqI	TCGA	7 cut(s)	42, 104, 138, 231, 387, 505, 557
TasI	AATT	5 cut(s)	95, 184, 398, 722, 796
TauI	GCSGC	3 cut(s)	204, 224, 539
TfiI	GAWTC	6 cut(s)	106, 331, 389, 511, 735, 851
TscAI	CASTG	1 cut(s)	915
TseFI	GTSAC	1 cut(s)	299
TseI	GCWGC	3 cut(s)	700, 861, 874
Tsp45I	GTSAC	1 cut(s)	299
TspDTI	ATGAA	6 cut(s)	381, 452, 524, 624, 705, 756
TspGWI	ACGGA	3 cut(s)	166, 297, 620
TspRI	CASTG	1 cut(s)	915
Tth111I	GACNNNGTC	1 cut(s)	263
VpaK11BI	GGWCC	3 cut(s)	19, 79, 157
XapI	RAATTY	3 cut(s)	95, 398, 796
XceI	RCATGY	1 cut(s)	822
XcmI	CCANNNNNNNNNTGG	1 cut(s)	127
XmiI	GTMKAC	1 cut(s)	267
XmnI	GAANNNNTTC	1 cut(s)	572
Zsp2I	ATGCAT	1 cut(s)	820

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.