MD14G1220700.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0028348
Physical Location & Seq
Reverse (-)
30256322 .. 30257851
1530 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1220700.v1.1.491

Sequence Viewer

Length: 1341 bp
ATGGCCAAAACCCTAATCCCAAATATTTTTCTTGTGCTTGTAGTTTTGATCTCCCTGGGCACGAATGCCCTAAGTGACAAAGGAGTCGCAAGGTTTCCTCTATACCATAGAGAAAAGTTCATGGCATCACATTTCAAGGACCATCACTCCCTCGTCAAAGCTCGATTGGCCCGGGATAGGGCACGCTTCAAACTCCTTCATCAACGTCATCTCCAGCACTCGAATTCTGCTCCATTTCTACGCCCAAACGTCGATTTCAATGAGACCAACGTGTTCGAGGAAGGCGGGGAGTATGTGACCGTTTTGAAAGTGGGAAATCCACCACAAAAAGTGCAACTTCTTGTAGACATGGGCACACCCTTCACATGGTGGCAGTGCCAACCAGGGTGCACCCAATGCTACTCAACACCACACCATATTTTTGATACGAGCATGTCATCCACGTACACTCAGACTGATTGCTCTAATGGATCATGTGATGTACAGCAGCTCTACATACAAGGTTGTGTTCGTCGTGGTGAGCATCAGTGTCGTTTCAGCGTTCATTATGTAGATCGATCTGTCTCCGAAGGTATCATGGCCACAGATACCATATCTACCGATGAGGGTGTGCATCTCAGTAACAATTTCTCTTTCGGATGTGGAAGTCGTATTACAGGTATAGACATGTTCAATGAGGCCGCGACTGGAATCATAGGCTTCGTACCGGGTGGATATTCATTTCCGACTCAACTCCAAGCTAACAATTTTACCTTCTTTCTGCCTTATTACAATACAACCGACGGTGGAACGTTGGACTTGAATTATTACGACGAATTCCGGTTTCGACATTCGAGTACAGCAATTCTGGACATCAGAATGGACCGTTATTATTTGTTGTTCAGTGGGATAGAAATGAACGAGGTAGCAATTCCAATTCCTCCTGAATTCTGGGAATTTGATTCCGATGGTGTTGGGGGTGTGACTATTGACACAGGGACTACAATCACCAAGTTTCCCGAGAAGGCCTATGACATATTCAAGGACTACTTCATCCGCAAGGCTCAGGGTTTGCCCCGAGCGCCCGGCGAAGATCAGTTAGACACTTGTTTTTATGTGAAAGGTCTCTACGAGTGGACTGACCTCGTTCCCAAGGTTAAATTTTTGTTTAAGGTGAGGAATCCGCCGTTGAAGTTGGACGTTCGTCAGGTTGTCATGGCTGTGAGCCACGACAAGTACTGCCTTGCATTTGCCGCCGGTGGAAGAACCTCAATAATTGGAAGCTGGCAGCTTCAGGGGTCTTATATTGGATTTAAGCTTCCTAGTCATGTTCTAGGAATTCAACCTAGAAGTTCTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

447

Amino Acids

50.44

Weight (kDa)

6.73

Isoelectric Point (pI)

27.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 98 - 264 2e-31 Xylanase inhibitor N-terminal
Asp PF00026 174 - 436 1.7e-06 Eukaryotic aspartyl protease
TAXi_C PF14541 290 - 440 5.1e-24 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028348)

Species Orthologous Gene IDs
malus_domestica MD14G1220700.v1.1
prunus_persica Prupe.5G216000_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 83
AccI GTMKAC 1 cut(s) 345
AccII CGCG 1 cut(s) 683
AciI CCGC 5 cut(s) 285, 681, 1036, 1163, 1233
AclI AACGTT 1 cut(s) 791
AclWI GGATC 1 cut(s) 478
AcoI YGGCCR 2 cut(s) 3, 579
AcsI RAATTY 6 cut(s) 223, 815, 926, 935, 1139, 1317
AcuI CTGAAG 1 cut(s) 1256
AdeI CACNNNGTG 1 cut(s) 369
AfaI GTAC 5 cut(s) 446, 483, 705, 838, 1217
AfiI CCNNNNNNNGG 3 cut(s) 177, 178, 366
AflIII ACRYGT 2 cut(s) 270, 666
AgsI TTSAA 9 cut(s) 136, 190, 259, 307, 673, 802, 1021, 1171, 1322
AjnI CCWGG 2 cut(s) 54, 382
AluBI AGCT 6 cut(s) 161, 490, 740, 1263, 1270, 1297
AluI AGCT 6 cut(s) 161, 490, 740, 1263, 1270, 1297
Alw21I GWGCWC 1 cut(s) 392
Alw26I GTCTC 3 cut(s) 257, 568, 1109
Alw44I GTGCAC 1 cut(s) 388
AlwI GGATC 1 cut(s) 478
Ama87I CYCGRG 3 cut(s) 171, 998, 1056
AoxI GGCC 5 cut(s) 3, 168, 579, 678, 1005
ApaLI GTGCAC 1 cut(s) 388
ApeKI GCWGC 2 cut(s) 487, 1267
ApoI RAATTY 6 cut(s) 223, 815, 926, 935, 1139, 1317
AspLEI GCGC 1 cut(s) 1063
AspS9I GGNCC 3 cut(s) 139, 169, 862
AsuC2I CCSGG 4 cut(s) 172, 173, 708, 1065
AsuHPI GGTGA 3 cut(s) 530, 979, 1165
AvaI CYCGRG 3 cut(s) 171, 998, 1056
AvaII GGWCC 2 cut(s) 139, 862
BaeGI GKGCMC 4 cut(s) 62, 184, 356, 392
BalI TGGCCA 2 cut(s) 5, 581
BarI GAAGNNNNNNTAC 2 cut(s) 637, 669
Bbv12I GWGCWC 1 cut(s) 392
BbvI GCAGC 2 cut(s) 499, 1279
BccI CCATC 2 cut(s) 150, 941
BceAI ACGGC 1 cut(s) 1150
BcgI CGANNNNNNTGC 2 cut(s) 512, 546
BciT130I CCWGG 2 cut(s) 56, 384
BcnI CCSGG 4 cut(s) 172, 173, 708, 1065
BcoDI GTCTC 3 cut(s) 257, 568, 1109
BfaI CTAG 3 cut(s) 1302, 1313, 1326
BfoI RGCGCY 1 cut(s) 1064
BisI GCNGC 4 cut(s) 488, 681, 1233, 1268
BlsI GCNGC 4 cut(s) 489, 682, 1234, 1269
BmcAI AGTACT 1 cut(s) 1217
Bme1390I CCNGG 6 cut(s) 56, 172, 173, 384, 708, 1065
Bme18I GGWCC 2 cut(s) 139, 862
BmeT110I CYCGRG 3 cut(s) 171, 998, 1056
BmgT120I GGNCC 3 cut(s) 139, 169, 862
BmrFI CCNGG 6 cut(s) 56, 172, 173, 384, 708, 1065
BmsI GCATC 3 cut(s) 134, 532, 622
BoxI GACNNNNGTC 1 cut(s) 1182
BpmI CTGGAG 1 cut(s) 197
Bpu10I CCTNAGC 1 cut(s) 1044
BpuMI CCSGG 4 cut(s) 172, 173, 708, 1065
Bsa29I ATCGAT 1 cut(s) 556
BsaAI YACGTR 1 cut(s) 444
BsaI GGTCTC 2 cut(s) 257, 1109
BsaJI CCNNGG 5 cut(s) 54, 55, 171, 383, 1131
BsaWI WCCGGW 1 cut(s) 819
BsaXI ACNNNNNCTCC 4 cut(s) 131, 161, 195, 225
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 178, 366
Bse118I RCCGGY 1 cut(s) 1235
Bse1I ACTGG 1 cut(s) 691
BseBI CCWGG 2 cut(s) 56, 384
BseCI ATCGAT 1 cut(s) 556
BseDI CCNNGG 5 cut(s) 54, 55, 171, 383, 1131
BseGI GGATG 3 cut(s) 437, 644, 1032
BseLI CCNNNNNNNGG 3 cut(s) 177, 178, 366
BseMII CTCAG 3 cut(s) 464, 631, 1058
BseNI ACTGG 1 cut(s) 691
BseSI GKGCMC 4 cut(s) 62, 184, 356, 392
BseXI GCAGC 2 cut(s) 499, 1279
Bsh1236I CGCG 1 cut(s) 683
BshFI GGCC 5 cut(s) 5, 170, 581, 680, 1007
BshVI ATCGAT 1 cut(s) 556
BsiHKAI GWGCWC 1 cut(s) 392
BsiHKCI CYCGRG 3 cut(s) 171, 998, 1056
BsiSI CCGG 5 cut(s) 172, 707, 820, 1065, 1236
BslFI GGGAC 1 cut(s) 991
BslI CCNNNNNNNGG 3 cut(s) 177, 178, 366
BsmAI GTCTC 3 cut(s) 257, 568, 1109
BsmFI GGGAC 1 cut(s) 991
BsmI GAATGC 1 cut(s) 70
BsnI GGCC 5 cut(s) 5, 170, 581, 680, 1007
Bso31I GGTCTC 2 cut(s) 257, 1109
BsoBI CYCGRG 3 cut(s) 171, 998, 1056
Bsp1286I GDGCHC 4 cut(s) 62, 184, 356, 392
Bsp1407I TGTACA 1 cut(s) 481
Bsp143I GATC 5 cut(s) 48, 470, 553, 557, 1072
BspACI CCGC 5 cut(s) 285, 681, 1036, 1163, 1233
BspANI GGCC 5 cut(s) 5, 170, 581, 680, 1007
BspCNI CTCAG 3 cut(s) 463, 630, 1057
BspDI ATCGAT 1 cut(s) 556
BspFNI CGCG 1 cut(s) 683
BspPI GGATC 1 cut(s) 478
BspTNI GGTCTC 2 cut(s) 257, 1109
BsrFI RCCGGY 1 cut(s) 1235
BsrGI TGTACA 1 cut(s) 481
BsrI ACTGG 1 cut(s) 691
BssAI RCCGGY 1 cut(s) 1235
BssECI CCNNGG 5 cut(s) 54, 55, 171, 383, 1131
BssMI GATC 5 cut(s) 48, 470, 553, 557, 1072
BssT1I CCWWGG 1 cut(s) 1131
Bst2UI CCWGG 2 cut(s) 56, 384
Bst4CI ACNGT 3 cut(s) 301, 785, 866
BstAPI GCANNNNNTGC 1 cut(s) 396
BstAUI TGTACA 1 cut(s) 481
BstBAI YACGTR 1 cut(s) 444
BstC8I GCNNGC 2 cut(s) 184, 1265
BstDEI CTNAG 4 cut(s) 71, 450, 617, 1044
BstF5I GGATG 3 cut(s) 437, 644, 1032
BstFNI CGCG 1 cut(s) 683
BstH2I RGCGCY 1 cut(s) 1064
BstHHI GCGC 1 cut(s) 1063
BstKTI GATC 5 cut(s) 51, 473, 556, 560, 1075
BstMAI GTCTC 3 cut(s) 257, 568, 1109
BstMBI GATC 5 cut(s) 48, 470, 553, 557, 1072
BstMWI GCNNNNNNNGC 4 cut(s) 167, 396, 1060, 1232
BstNI CCWGG 2 cut(s) 56, 384
BstNSI RCATGY 2 cut(s) 436, 670
BstPAI GACNNNNGTC 1 cut(s) 1182
BstSCI CCNGG 6 cut(s) 54, 170, 171, 382, 706, 1063
BstSLI GKGCMC 4 cut(s) 62, 184, 356, 392
BstUI CGCG 1 cut(s) 683
BstV1I GCAGC 2 cut(s) 499, 1279
Bsu15I ATCGAT 1 cut(s) 556
BsuRI GGCC 5 cut(s) 5, 170, 581, 680, 1007
BsuTUI ATCGAT 1 cut(s) 556
BtsCI GGATG 3 cut(s) 437, 644, 1032
BtsI GCAGTG 1 cut(s) 380
BtsIMutI CAGTG 3 cut(s) 380, 533, 889
Cac8I GCNNGC 2 cut(s) 184, 1265
CfoI GCGC 1 cut(s) 1063
Cfr10I RCCGGY 1 cut(s) 1235
Cfr13I GGNCC 3 cut(s) 139, 169, 862
Cfr9I CCCGGG 1 cut(s) 171
ClaI ATCGAT 1 cut(s) 556
Csp6I GTAC 5 cut(s) 445, 482, 704, 837, 1216
CviAII CATG 9 cut(s) 121, 349, 366, 433, 474, 577, 667, 1195, 1307
CviQI GTAC 5 cut(s) 445, 482, 704, 837, 1216
DdeI CTNAG 4 cut(s) 71, 450, 617, 1044
DpnI GATC 5 cut(s) 50, 472, 555, 559, 1074
DpnII GATC 5 cut(s) 48, 470, 553, 557, 1072
DraIII CACNNNGTG 1 cut(s) 369
DrdI GACNNNNNNGTC 1 cut(s) 83
DseDI GACNNNNNNGTC 1 cut(s) 83
EaeI YGGCCR 2 cut(s) 3, 579
EciI GGCGGA 1 cut(s) 1152
Eco130I CCWWGG 1 cut(s) 1131
Eco147I AGGCCT 1 cut(s) 1007
Eco31I GGTCTC 2 cut(s) 257, 1109
Eco47I GGWCC 2 cut(s) 139, 862
Eco57I CTGAAG 1 cut(s) 1256
Eco88I CYCGRG 3 cut(s) 171, 998, 1056
EcoRI GAATTC 4 cut(s) 223, 815, 926, 1317
EcoRII CCWGG 2 cut(s) 54, 382
EcoT14I CCWWGG 1 cut(s) 1131
ErhI CCWWGG 1 cut(s) 1131
FaeI CATG 9 cut(s) 124, 352, 369, 436, 477, 580, 670, 1198, 1310
FalI AAGNNNNNCTT 4 cut(s) 321, 353, 1013, 1045
FaqI GGGAC 1 cut(s) 991
FatI CATG 9 cut(s) 120, 348, 365, 432, 473, 576, 666, 1194, 1306
FauI CCCGC 1 cut(s) 278
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 4 cut(s) 488, 681, 1233, 1268
FokI GGATG 3 cut(s) 424, 651, 1019
Fsp4HI GCNGC 4 cut(s) 488, 681, 1233, 1268
FspBI CTAG 3 cut(s) 1302, 1313, 1326
GlaI GCGC 1 cut(s) 1062
GluI GCNGC 4 cut(s) 488, 681, 1233, 1268
GsuI CTGGAG 1 cut(s) 197
HaeII RGCGCY 1 cut(s) 1064
HaeIII GGCC 5 cut(s) 5, 170, 581, 680, 1007
HapII CCGG 5 cut(s) 172, 707, 820, 1065, 1236
HhaI GCGC 1 cut(s) 1063
Hin1II CATG 9 cut(s) 124, 352, 369, 436, 477, 580, 670, 1198, 1310
Hin6I GCGC 1 cut(s) 1061
HinP1I GCGC 1 cut(s) 1061
HindIII AAGCTT 1 cut(s) 1295
HinfI GANTC 5 cut(s) 84, 690, 727, 941, 1159
HpaII CCGG 5 cut(s) 172, 707, 820, 1065, 1236
HphI GGTGA 3 cut(s) 530, 979, 1165
Hpy166II GTNNAC 4 cut(s) 346, 390, 447, 1116
Hpy188I TCNGA 6 cut(s) 453, 568, 638, 726, 857, 946
Hpy188III TCNNGA 3 cut(s) 848, 923, 998
Hpy8I GTNNAC 4 cut(s) 346, 390, 447, 1116
Hpy99I CGWCG 4 cut(s) 254, 516, 785, 815
HpyAV CCTTC 6 cut(s) 206, 275, 370, 563, 763, 997
HpyCH4III ACNGT 3 cut(s) 301, 785, 866
HpyCH4IV ACGT 6 cut(s) 205, 249, 270, 443, 791, 1179
HpyCH4V TGCA 4 cut(s) 334, 390, 613, 1226
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 396, 1060, 1232
HpyF3I CTNAG 4 cut(s) 71, 450, 617, 1044
HpySE526I ACGT 6 cut(s) 205, 249, 270, 443, 791, 1179
Hsp92II CATG 9 cut(s) 124, 352, 369, 436, 477, 580, 670, 1198, 1310
HspAI GCGC 1 cut(s) 1061
Kzo9I GATC 5 cut(s) 48, 470, 553, 557, 1072
LmnI GCTCC 1 cut(s) 235
Lsp1109I GCAGC 2 cut(s) 499, 1279
LweI GCATC 3 cut(s) 134, 532, 622
MaeI CTAG 3 cut(s) 1302, 1313, 1326
MaeII ACGT 6 cut(s) 205, 249, 270, 443, 791, 1179
MaeIII GTNAC 4 cut(s) 74, 295, 620, 961
MalI GATC 5 cut(s) 50, 472, 555, 559, 1074
MboI GATC 5 cut(s) 48, 470, 553, 557, 1072
MboII GAAGA 2 cut(s) 1082, 1254
MhlI GDGCHC 4 cut(s) 62, 184, 356, 392
MlsI TGGCCA 2 cut(s) 5, 581
MluNI TGGCCA 2 cut(s) 5, 581
MlyI GAGTC 2 cut(s) 93, 721
MmeI TCCRAC 3 cut(s) 749, 774, 1155
Mox20I TGGCCA 2 cut(s) 5, 581
MscI TGGCCA 2 cut(s) 5, 581
MseI TTAA 3 cut(s) 1137, 1149, 1293
MslI CAYNNNNRTG 2 cut(s) 857, 1199
Msp20I TGGCCA 2 cut(s) 5, 581
MspI CCGG 5 cut(s) 172, 707, 820, 1065, 1236
MspR9I CCNGG 6 cut(s) 56, 172, 173, 384, 708, 1065
Mva1269I GAATGC 1 cut(s) 70
MvaI CCWGG 2 cut(s) 56, 384
MvnI CGCG 1 cut(s) 683
MwoI GCNNNNNNNGC 4 cut(s) 167, 396, 1060, 1232
NciI CCSGG 4 cut(s) 172, 173, 708, 1065
NdeII GATC 5 cut(s) 48, 470, 553, 557, 1072
NlaIII CATG 9 cut(s) 124, 352, 369, 436, 477, 580, 670, 1198, 1310
NmuCI GTSAC 3 cut(s) 74, 295, 961
NspI RCATGY 2 cut(s) 436, 670
PasI CCCWGGG 1 cut(s) 55
PceI AGGCCT 1 cut(s) 1007
PciI ACATGT 1 cut(s) 666
PctI GAATGC 1 cut(s) 70
PfeI GAWTC 3 cut(s) 690, 941, 1159
PkrI GCNGC 4 cut(s) 489, 682, 1234, 1269
PleI GAGTC 2 cut(s) 92, 721
PpsI GAGTC 2 cut(s) 92, 721
Ppu21I YACGTR 1 cut(s) 444
PscI ACATGT 1 cut(s) 666
PshAI GACNNNNGTC 1 cut(s) 1182
Psp1406I AACGTT 1 cut(s) 791
Psp6I CCWGG 2 cut(s) 54, 382
PspGI CCWGG 2 cut(s) 54, 382
PspPI GGNCC 3 cut(s) 139, 169, 862
RsaI GTAC 5 cut(s) 446, 483, 705, 838, 1217
RsaNI GTAC 5 cut(s) 445, 482, 704, 837, 1216
RseI CAYNNNNRTG 2 cut(s) 857, 1199
SaqAI TTAA 3 cut(s) 1137, 1149, 1293
SatI GCNGC 4 cut(s) 488, 681, 1233, 1268
Sau3AI GATC 5 cut(s) 48, 470, 553, 557, 1072
Sau96I GGNCC 3 cut(s) 139, 169, 862
ScaI AGTACT 1 cut(s) 1217
SchI GAGTC 2 cut(s) 93, 721
ScrFI CCNGG 6 cut(s) 56, 172, 173, 384, 708, 1065
SduI GDGCHC 4 cut(s) 62, 184, 356, 392
SfaNI GCATC 3 cut(s) 134, 532, 622
SgrAI CRCCGGYG 1 cut(s) 1235
SinI GGWCC 2 cut(s) 139, 862
SmaI CCCGGG 1 cut(s) 173
SmiMI CAYNNNNRTG 2 cut(s) 857, 1199
SseBI AGGCCT 1 cut(s) 1007
SsiI CCGC 5 cut(s) 285, 681, 1036, 1163, 1233
SspI AATATT 1 cut(s) 25
SspMI CTAG 3 cut(s) 1302, 1313, 1326
StuI AGGCCT 1 cut(s) 1007
StyD4I CCNGG 6 cut(s) 54, 170, 171, 382, 706, 1063
StyI CCWWGG 1 cut(s) 1131
TaaI ACNGT 3 cut(s) 301, 785, 866
TaiI ACGT 6 cut(s) 208, 252, 273, 446, 794, 1182
TaqI TCGA 7 cut(s) 163, 221, 252, 276, 556, 826, 833
TatI WGTACW 3 cut(s) 481, 836, 1215
TauI GCSGC 2 cut(s) 683, 1235
TfiI GAWTC 3 cut(s) 690, 941, 1159
Tru1I TTAA 3 cut(s) 1137, 1149, 1293
Tru9I TTAA 3 cut(s) 1137, 1149, 1293
TscAI CASTG 3 cut(s) 380, 533, 889
TseFI GTSAC 3 cut(s) 74, 295, 961
TseI GCWGC 2 cut(s) 487, 1267
Tsp45I GTSAC 3 cut(s) 74, 295, 961
TspDTI ATGAA 6 cut(s) 109, 188, 533, 708, 911, 1021
TspMI CCCGGG 1 cut(s) 171
TspRI CASTG 3 cut(s) 380, 533, 889
VneI GTGCAC 1 cut(s) 388
VpaK11BI GGWCC 2 cut(s) 139, 862
XapI RAATTY 6 cut(s) 223, 815, 926, 935, 1139, 1317
XceI RCATGY 2 cut(s) 436, 670
XmaI CCCGGG 1 cut(s) 171
XmiI GTMKAC 1 cut(s) 345
XspI CTAG 3 cut(s) 1302, 1313, 1326
ZrmI AGTACT 1 cut(s) 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.