Prupe.5G216000_v2.0.a1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0028348
Physical Location & Seq
Reverse (-)
16960577 .. 16963287
2711 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G216000.1

Sequence Viewer

Length: 1278 bp
ATGGGGGGCTTGGTCACTTTGATTATTAAGTCACTCAACGACGAGAAAAAAGGAGTCGTGGCAGAAAAATTCATAGCTTCAAACTTCAAGGACCAACGCTCCCTTGTCCGGACTCGTCTGGCCCGCGACAATGCGCGCTTTCATCGTCGTCATGCATCATCTTCTGCTCAGTTTCACAAGCACTCTGGGAATGATCTTAACTATACTGCCGTGCTCGAGGAAGGCGGGGAGTACGTCACCGTTTTGAAAGTGGGAAGTCCGTCGCAGAACGTGCAGCTGCTTGTGGACATAGGCACGCCTTTGACATGGTGGCAGTGCCAGCCCAACTGCAAGTATTGCTACTCCACGCCACACCACATTTTTGATACGAGCACGTCGTCCACGTATACTGAGACGGATTGCGCCGAGGGATTCTGCGATGTAACCCAGCTTTTTATACTGGGTTGCATTCGCCGCGACGATCCCAAGTGCCATTACAGGGTTCAATACGTCGACGGGTCTGTCTCCCAAGGCGTCATGGCGAGAGATGTTGTCGCCACCTCTGAGGGTGTGACTCTCCATAACTCTTTCTCTTTCGGATGTGGACGAAGCAATCAAGGTGTGACAATGTTCAATGCGGACGCTACGGGGATCCTAGGGTTTGTACCGGGCGGATATTCGTTTCCCTCTCAACTCCAGGTTAACACATTCAGCTTCTGTCTCCCTACTTACAATACCACTAAGAGTGGAAGTCTGTACCTGAATTATTACCCGTCGGCTTTTCAGTATTCAAGTAGATCGATGTTGATTATTAGAACGGGCCACTATTATTTGGATTTCAGCGGGGTAGAAATTAACCAGGAAGCAATTCCAATTCCTCCAGAGAACTGGCAATGTGACCGGGAAGGGGTTGGCGGTGTGATTATTGACACAGGGAGCACCATAACCAGGTTCCCCAAGAAAGCCTATGACATATTCAAAGACTATTTCCTGCGTAACGCTCAGGGCTTGCCTTTATCGCAACCCGAAGAGCAGTTAGATACTTGCTTCAATGTGGCAGGTGTCGGAGAGTGGGAAGGCTTCGTCCCTAAGGTTAGATTTTTGTTCAAGTGGCCGTCTGGTCGGCCCCTGGAGTTCGAGACAGGTCAGCTTCTGATGTCGGTGGGCAATAACAAATATTGCCTTGCATTTGGCGCAGGAGGACCCACCACAATCATTGGGAGTTGGCAGCTACAAGGCTCGCTTGTTGGCTTCGATCTTCCTAATCATCTTGTCGGATTTCTTCGTGCAAGTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

426

Amino Acids

47.14

Weight (kDa)

6.55

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028348)

Species Orthologous Gene IDs
malus_domestica MD14G1220700.v1.1
prunus_persica Prupe.5G216000_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1028
AasI GACNNNNNNGTC 1 cut(s) 500
Acc36I ACCTGC 1 cut(s) 1028
AccI GTMKAC 2 cut(s) 386, 492
AccII CGCG 3 cut(s) 126, 136, 456
AccIII TCCGGA 1 cut(s) 108
AciI CCGC 7 cut(s) 124, 225, 454, 617, 651, 822, 894
AclWI GGATC 3 cut(s) 455, 625, 638
AcoI YGGCCR 1 cut(s) 1091
AcsI RAATTY 1 cut(s) 68
AcyI GRCGYC 1 cut(s) 513
AfaI GTAC 3 cut(s) 233, 645, 737
AfiI CCNNNNNNNGG 4 cut(s) 108, 478, 886, 927
AgsI TTSAA 9 cut(s) 81, 88, 247, 485, 613, 771, 958, 1030, 1087
AjiI CACGTC 1 cut(s) 375
AjnI CCWGG 4 cut(s) 675, 837, 926, 1107
AluBI AGCT 6 cut(s) 77, 277, 430, 693, 1129, 1210
AluI AGCT 6 cut(s) 77, 277, 430, 693, 1129, 1210
Alw21I GWGCWC 3 cut(s) 216, 374, 920
Alw26I GTCTC 4 cut(s) 386, 508, 704, 1112
AlwI GGATC 3 cut(s) 455, 625, 638
AlwNI CAGNNNCTG 2 cut(s) 696, 1132
Ama87I CYCGRG 1 cut(s) 215
Aor13HI TCCGGA 1 cut(s) 108
AoxI GGCC 4 cut(s) 120, 799, 1091, 1103
ApeKI GCWGC 3 cut(s) 274, 277, 1207
ApoI RAATTY 1 cut(s) 68
Asp700I GAANNNNTTC 1 cut(s) 846
AspA2I CCTAGG 1 cut(s) 634
AspLEI GCGC 4 cut(s) 136, 138, 404, 1175
AspS9I GGNCC 5 cut(s) 91, 121, 799, 1104, 1181
AsuC2I CCSGG 2 cut(s) 648, 881
AsuHPI GGTGA 1 cut(s) 229
AvaI CYCGRG 1 cut(s) 215
AvaII GGWCC 2 cut(s) 91, 1181
AvrII CCTAGG 1 cut(s) 634
AxyI CCTNAGG 1 cut(s) 1068
BamHI GGATCC 1 cut(s) 630
Bbv12I GWGCWC 3 cut(s) 216, 374, 920
BbvI GCAGC 3 cut(s) 264, 286, 1219
BceAI ACGGC 2 cut(s) 194, 1078
BciT130I CCWGG 4 cut(s) 677, 839, 928, 1109
BcnI CCSGG 2 cut(s) 648, 881
BcoDI GTCTC 4 cut(s) 386, 508, 704, 1112
BfaI CTAG 1 cut(s) 635
BfuAI ACCTGC 1 cut(s) 1028
BisI GCNGC 4 cut(s) 275, 278, 454, 1208
BlnI CCTAGG 1 cut(s) 634
BlsI GCNGC 4 cut(s) 276, 279, 455, 1209
Bme1390I CCNGG 6 cut(s) 648, 677, 839, 881, 928, 1109
Bme18I GGWCC 2 cut(s) 91, 1181
BmeT110I CYCGRG 1 cut(s) 215
BmgBI CACGTC 1 cut(s) 375
BmgT120I GGNCC 5 cut(s) 91, 121, 799, 1104, 1181
BmiI GGNNCC 4 cut(s) 632, 932, 1106, 1183
BmrFI CCNGG 6 cut(s) 648, 677, 839, 881, 928, 1109
BmrI ACTGGG 1 cut(s) 449
BmsI GCATC 1 cut(s) 164
BmuI ACTGGG 1 cut(s) 449
BpmI CTGGAG 3 cut(s) 659, 843, 1130
Bpu10I CCTNAGC 1 cut(s) 981
BpuMI CCSGG 2 cut(s) 648, 881
Bsa29I ATCGAT 1 cut(s) 779
BsaAI YACGTR 1 cut(s) 384
BsaHI GRCGYC 1 cut(s) 513
BsaJI CCNNGG 4 cut(s) 405, 508, 634, 1107
BsaWI WCCGGW 1 cut(s) 108
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bsc4I CCNNNNNNNGG 4 cut(s) 108, 478, 886, 927
Bse1I ACTGG 2 cut(s) 444, 872
Bse21I CCTNAGG 1 cut(s) 1068
Bse3DI GCAATG 1 cut(s) 878
BseAI TCCGGA 1 cut(s) 108
BseBI CCWGG 4 cut(s) 677, 839, 928, 1109
BseCI ATCGAT 1 cut(s) 779
BseDI CCNNGG 4 cut(s) 405, 508, 634, 1107
BseGI GGATG 1 cut(s) 584
BseLI CCNNNNNNNGG 4 cut(s) 108, 478, 886, 927
BseMI GCAATG 1 cut(s) 878
BseMII CTCAG 4 cut(s) 182, 381, 534, 995
BseNI ACTGG 2 cut(s) 444, 872
BsePI GCGCGC 1 cut(s) 134
BseXI GCAGC 3 cut(s) 264, 286, 1219
BseYI CCCAGC 1 cut(s) 426
BsgI GTGCAG 1 cut(s) 293
Bsh1236I CGCG 3 cut(s) 126, 136, 456
BshFI GGCC 4 cut(s) 122, 801, 1093, 1105
BshVI ATCGAT 1 cut(s) 779
BsiHKAI GWGCWC 3 cut(s) 216, 374, 920
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 3 cut(s) 109, 647, 880
BslFI GGGAC 1 cut(s) 1049
BslI CCNNNNNNNGG 4 cut(s) 108, 478, 886, 927
BsmAI GTCTC 4 cut(s) 386, 508, 704, 1112
BsmBI CGTCTC 1 cut(s) 386
BsmFI GGGAC 1 cut(s) 1049
BsmI GAATGC 1 cut(s) 447
BsnI GGCC 4 cut(s) 122, 801, 1093, 1105
BsoBI CYCGRG 1 cut(s) 215
Bsp1286I GDGCHC 3 cut(s) 216, 374, 920
Bsp13I TCCGGA 1 cut(s) 108
Bsp143I GATC 5 cut(s) 193, 460, 630, 776, 1234
BspACI CCGC 7 cut(s) 124, 225, 454, 617, 651, 822, 894
BspANI GGCC 4 cut(s) 122, 801, 1093, 1105
BspCNI CTCAG 4 cut(s) 181, 382, 535, 994
BspDI ATCGAT 1 cut(s) 779
BspEI TCCGGA 1 cut(s) 108
BspFNI CGCG 3 cut(s) 126, 136, 456
BspLI GGNNCC 4 cut(s) 632, 932, 1106, 1183
BspMI ACCTGC 1 cut(s) 1028
BspPI GGATC 3 cut(s) 455, 625, 638
BspQI GCTCTTC 1 cut(s) 1002
BsrDI GCAATG 1 cut(s) 878
BsrI ACTGG 2 cut(s) 444, 872
BssECI CCNNGG 4 cut(s) 405, 508, 634, 1107
BssHII GCGCGC 1 cut(s) 134
BssMI GATC 5 cut(s) 193, 460, 630, 776, 1234
BssNAI GTATAC 1 cut(s) 387
BssNI GRCGYC 1 cut(s) 513
BssT1I CCWWGG 2 cut(s) 508, 634
Bst1107I GTATAC 1 cut(s) 387
Bst2UI CCWGG 4 cut(s) 677, 839, 928, 1109
Bst4CI ACNGT 1 cut(s) 241
Bst6I CTCTTC 1 cut(s) 1002
BstACI GRCGYC 1 cut(s) 513
BstAPI GCANNNNNTGC 2 cut(s) 271, 336
BstBAI YACGTR 1 cut(s) 384
BstC8I GCNNGC 6 cut(s) 124, 136, 296, 320, 989, 1220
BstDEI CTNAG 6 cut(s) 168, 390, 543, 720, 981, 1068
BstF5I GGATG 1 cut(s) 584
BstFNI CGCG 3 cut(s) 126, 136, 456
BstHHI GCGC 4 cut(s) 136, 138, 404, 1175
BstKTI GATC 5 cut(s) 196, 463, 633, 779, 1237
BstMAI GTCTC 4 cut(s) 386, 508, 704, 1112
BstMBI GATC 5 cut(s) 193, 460, 630, 776, 1234
BstMWI GCNNNNNNNGC 6 cut(s) 271, 319, 336, 453, 997, 1172
BstNI CCWGG 4 cut(s) 677, 839, 928, 1109
BstSCI CCNGG 6 cut(s) 646, 675, 837, 879, 926, 1107
BstUI CGCG 3 cut(s) 126, 136, 456
BstV1I GCAGC 3 cut(s) 264, 286, 1219
BstX2I RGATCY 1 cut(s) 630
BstXI CCANNNNNNTGG 1 cut(s) 867
BstYI RGATCY 1 cut(s) 630
BstZ17I GTATAC 1 cut(s) 387
Bsu15I ATCGAT 1 cut(s) 779
Bsu36I CCTNAGG 1 cut(s) 1068
BsuRI GGCC 4 cut(s) 122, 801, 1093, 1105
BsuTUI ATCGAT 1 cut(s) 779
BtgZI GCGATG 1 cut(s) 432
BtrI CACGTC 1 cut(s) 375
BtsCI GGATG 1 cut(s) 584
BtsI GCAGTG 1 cut(s) 320
BtsIMutI CAGTG 1 cut(s) 320
BveI ACCTGC 1 cut(s) 1028
Cac8I GCNNGC 6 cut(s) 124, 136, 296, 320, 989, 1220
CaiI CAGNNNCTG 2 cut(s) 696, 1132
CfoI GCGC 4 cut(s) 136, 138, 404, 1175
Cfr13I GGNCC 5 cut(s) 91, 121, 799, 1104, 1181
ClaI ATCGAT 1 cut(s) 779
CseI GACGC 2 cut(s) 502, 629
CsiI ACCWGGT 1 cut(s) 926
Csp6I GTAC 3 cut(s) 232, 644, 736
CspCI CAANNNNNGTGG 2 cut(s) 1177, 1212
CviAII CATG 3 cut(s) 152, 306, 517
CviQI GTAC 3 cut(s) 232, 644, 736
DdeI CTNAG 6 cut(s) 168, 390, 543, 720, 981, 1068
DpnI GATC 5 cut(s) 195, 462, 632, 778, 1236
DpnII GATC 5 cut(s) 193, 460, 630, 776, 1234
DrdI GACNNNNNNGTC 1 cut(s) 500
DseDI GACNNNNNNGTC 1 cut(s) 500
EaeI YGGCCR 1 cut(s) 1091
Eam1104I CTCTTC 1 cut(s) 1002
EarI CTCTTC 1 cut(s) 1002
EciI GGCGGA 1 cut(s) 666
Eco130I CCWWGG 2 cut(s) 508, 634
Eco47I GGWCC 2 cut(s) 91, 1181
Eco81I CCTNAGG 1 cut(s) 1068
Eco88I CYCGRG 1 cut(s) 215
EcoO109I RGGNCCY 1 cut(s) 1181
EcoRII CCWGG 4 cut(s) 675, 837, 926, 1107
EcoT14I CCWWGG 2 cut(s) 508, 634
EcoT22I ATGCAT 1 cut(s) 157
ErhI CCWWGG 2 cut(s) 508, 634
Esp3I CGTCTC 1 cut(s) 386
FaeI CATG 3 cut(s) 155, 309, 520
FalI AAGNNNNNCTT 2 cut(s) 1206, 1238
FaqI GGGAC 1 cut(s) 1049
FatI CATG 3 cut(s) 151, 305, 516
FauI CCCGC 3 cut(s) 131, 218, 815
FblI GTMKAC 2 cut(s) 386, 492
Fnu4HI GCNGC 4 cut(s) 275, 278, 454, 1208
FokI GGATG 1 cut(s) 591
Fsp4HI GCNGC 4 cut(s) 275, 278, 454, 1208
FspBI CTAG 1 cut(s) 635
GlaI GCGC 4 cut(s) 135, 137, 403, 1174
GluI GCNGC 4 cut(s) 275, 278, 454, 1208
GsaI CCCAGC 1 cut(s) 430
GsuI CTGGAG 3 cut(s) 659, 843, 1130
HaeIII GGCC 4 cut(s) 122, 801, 1093, 1105
HapII CCGG 3 cut(s) 109, 647, 880
HgaI GACGC 2 cut(s) 502, 629
HhaI GCGC 4 cut(s) 136, 138, 404, 1175
Hin1I GRCGYC 1 cut(s) 513
Hin1II CATG 3 cut(s) 155, 309, 520
Hin6I GCGC 4 cut(s) 134, 136, 402, 1173
HinP1I GCGC 4 cut(s) 134, 136, 402, 1173
HincII GTYRAC 2 cut(s) 493, 682
HindII GTYRAC 2 cut(s) 493, 682
HinfI GANTC 4 cut(s) 54, 112, 411, 553
HpaI GTTAAC 1 cut(s) 682
HpaII CCGG 3 cut(s) 109, 647, 880
HphI GGTGA 1 cut(s) 229
Hpy166II GTNNAC 6 cut(s) 286, 381, 387, 493, 584, 682
Hpy188I TCNGA 5 cut(s) 544, 578, 1046, 1134, 1256
Hpy188III TCNNGA 3 cut(s) 109, 860, 1117
Hpy8I GTNNAC 6 cut(s) 286, 381, 387, 493, 584, 682
Hpy99I CGWCG 8 cut(s) 44, 150, 265, 379, 461, 494, 497, 757
HpyAV CCTTC 3 cut(s) 215, 878, 1049
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4IV ACGT 5 cut(s) 234, 270, 374, 383, 489
HpyCH4V TGCA 6 cut(s) 155, 274, 330, 447, 1166, 1268
HpyF10VI GCNNNNNNNGC 6 cut(s) 271, 319, 336, 453, 997, 1172
HpyF3I CTNAG 6 cut(s) 168, 390, 543, 720, 981, 1068
HpySE526I ACGT 5 cut(s) 234, 270, 374, 383, 489
Hsp92I GRCGYC 1 cut(s) 513
Hsp92II CATG 3 cut(s) 155, 309, 520
HspAI GCGC 4 cut(s) 134, 136, 402, 1173
Kpn2I TCCGGA 1 cut(s) 108
KspAI GTTAAC 1 cut(s) 682
Kzo9I GATC 5 cut(s) 193, 460, 630, 776, 1234
LguI GCTCTTC 1 cut(s) 1002
LmnI GCTCC 2 cut(s) 104, 915
Lsp1109I GCAGC 3 cut(s) 264, 286, 1219
LweI GCATC 1 cut(s) 164
MabI ACCWGGT 1 cut(s) 926
MaeI CTAG 1 cut(s) 635
MaeII ACGT 5 cut(s) 234, 270, 374, 383, 489
MaeIII GTNAC 8 cut(s) 13, 30, 235, 421, 550, 601, 875, 974
MalI GATC 5 cut(s) 195, 462, 632, 778, 1236
MboI GATC 5 cut(s) 193, 460, 630, 776, 1234
MboII GAAGA 4 cut(s) 153, 1019, 1229, 1253
MflI RGATCY 1 cut(s) 630
MhlI GDGCHC 3 cut(s) 216, 374, 920
MluCI AATT 5 cut(s) 68, 742, 831, 846, 852
MlyI GAGTC 3 cut(s) 63, 106, 547
MmeI TCCRAC 2 cut(s) 1024, 1234
MnlI CCTC 7 cut(s) 211, 400, 538, 550, 676, 867, 1172
Mph1103I ATGCAT 1 cut(s) 157
MroI TCCGGA 1 cut(s) 108
MroXI GAANNNNTTC 1 cut(s) 846
MseI TTAA 5 cut(s) 27, 198, 681, 834, 1276
MspA1I CMGCKG 2 cut(s) 277, 822
MspI CCGG 3 cut(s) 109, 647, 880
MspR9I CCNGG 6 cut(s) 648, 677, 839, 881, 928, 1109
Mva1269I GAATGC 1 cut(s) 447
MvaI CCWGG 4 cut(s) 677, 839, 928, 1109
MvnI CGCG 3 cut(s) 126, 136, 456
MwoI GCNNNNNNNGC 6 cut(s) 271, 319, 336, 453, 997, 1172
NciI CCSGG 2 cut(s) 648, 881
NdeII GATC 5 cut(s) 193, 460, 630, 776, 1234
NlaIII CATG 3 cut(s) 155, 309, 520
NlaIV GGNNCC 4 cut(s) 632, 932, 1106, 1183
NmeAIII GCCGAG 1 cut(s) 430
NmuCI GTSAC 6 cut(s) 13, 30, 235, 550, 601, 875
NsiI ATGCAT 1 cut(s) 157
PaeR7I CTCGAG 1 cut(s) 215
PaqCI CACCTGC 1 cut(s) 1028
PauI GCGCGC 1 cut(s) 134
PciSI GCTCTTC 1 cut(s) 1002
PcsI WCGNNNNNNNCGW 2 cut(s) 374, 380
PctI GAATGC 1 cut(s) 447
PdmI GAANNNNTTC 1 cut(s) 846
PfeI GAWTC 1 cut(s) 411
PkrI GCNGC 4 cut(s) 276, 279, 455, 1209
PleI GAGTC 3 cut(s) 62, 106, 547
PpsI GAGTC 3 cut(s) 62, 106, 547
Ppu21I YACGTR 1 cut(s) 384
PpuMI RGGWCCY 1 cut(s) 1181
Psp5II RGGWCCY 1 cut(s) 1181
Psp6I CCWGG 4 cut(s) 675, 837, 926, 1107
PspFI CCCAGC 1 cut(s) 426
PspGI CCWGG 4 cut(s) 675, 837, 926, 1107
PspN4I GGNNCC 4 cut(s) 632, 932, 1106, 1183
PspPI GGNCC 5 cut(s) 91, 121, 799, 1104, 1181
PspPPI RGGWCCY 1 cut(s) 1181
PspXI VCTCGAGB 1 cut(s) 215
PstNI CAGNNNCTG 2 cut(s) 696, 1132
PsuI RGATCY 1 cut(s) 630
PteI GCGCGC 1 cut(s) 134
PvuII CAGCTG 1 cut(s) 277
RsaI GTAC 3 cut(s) 233, 645, 737
RsaNI GTAC 3 cut(s) 232, 644, 736
SalI GTCGAC 1 cut(s) 491
SapI GCTCTTC 1 cut(s) 1002
SaqAI TTAA 5 cut(s) 27, 198, 681, 834, 1276
SatI GCNGC 4 cut(s) 275, 278, 454, 1208
Sau3AI GATC 5 cut(s) 193, 460, 630, 776, 1234
Sau96I GGNCC 5 cut(s) 91, 121, 799, 1104, 1181
SchI GAGTC 3 cut(s) 63, 106, 547
ScrFI CCNGG 6 cut(s) 648, 677, 839, 881, 928, 1109
SduI GDGCHC 3 cut(s) 216, 374, 920
SexAI ACCWGGT 1 cut(s) 926
SfaNI GCATC 1 cut(s) 164
Sfr274I CTCGAG 1 cut(s) 215
SgrDI CGTCGACG 1 cut(s) 491
SinI GGWCC 2 cut(s) 91, 1181
SlaI CTCGAG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 5 cut(s) 68, 742, 831, 846, 852
SsiI CCGC 7 cut(s) 124, 225, 454, 617, 651, 822, 894
SspI AATATT 1 cut(s) 1157
SspMI CTAG 1 cut(s) 635
StyD4I CCNGG 6 cut(s) 646, 675, 837, 879, 926, 1107
StyI CCWWGG 2 cut(s) 508, 634
TaaI ACNGT 1 cut(s) 241
TaiI ACGT 5 cut(s) 237, 273, 377, 386, 492
TaqI TCGA 5 cut(s) 216, 492, 779, 1116, 1233
TasI AATT 5 cut(s) 68, 742, 831, 846, 852
TauI GCSGC 1 cut(s) 456
TfiI GAWTC 1 cut(s) 411
Tru1I TTAA 5 cut(s) 27, 198, 681, 834, 1276
Tru9I TTAA 5 cut(s) 27, 198, 681, 834, 1276
TscAI CASTG 1 cut(s) 320
TseFI GTSAC 6 cut(s) 13, 30, 235, 550, 601, 875
TseI GCWGC 3 cut(s) 274, 277, 1207
Tsp45I GTSAC 6 cut(s) 13, 30, 235, 550, 601, 875
TspDTI ATGAA 2 cut(s) 61, 131
TspGWI ACGGA 2 cut(s) 249, 410
TspRI CASTG 1 cut(s) 320
VpaK11BI GGWCC 2 cut(s) 91, 1181
XapI RAATTY 1 cut(s) 68
XhoI CTCGAG 1 cut(s) 215
XmaJI CCTAGG 1 cut(s) 634
XmiI GTMKAC 2 cut(s) 386, 492
XmnI GAANNNNTTC 1 cut(s) 846
XspI CTAG 1 cut(s) 635
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.