MD15G1009700.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0021153
Physical Location & Seq
Forward (+)
525399 .. 525754
356 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1009700.v1.1.491

Sequence Viewer

Length: 264 bp
ATGTTACGATTTTTTTTTTCATGTACAGTGGAAGCATTCGGAATCAAGGAGCTGTTGCCGGCATATTTGGATCCAACCCTGCAGCCTAATGACCTCCTTACCGGTGTTGTCATAGCTGCCGGTGGTGCAGGTTATGATCCATTGACAGCAAAACTTGCGGCAGTTGCATCGCTATCTGACCAATTGAAACAGTTCAAGGAGTACATAGAAAAGCTAAAAGGAATTGTTGGAGAGGAGAAACGACTAGAATTTCTGATGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

88

Amino Acids

9.55

Weight (kDa)

4.99

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021153)

Species Orthologous Gene IDs
malus_domestica MD15G1009700.v1.1
pyrus_communis pycom01g13310
rosa_samantha Rh2AG124300 Rh2BG127500 Rh2CG128400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 119
AciI CCGC 1 cut(s) 158
AclWI GGATC 3 cut(s) 65, 78, 131
AcsI RAATTY 1 cut(s) 248
AfaI GTAC 2 cut(s) 25, 203
AgeI ACCGGT 1 cut(s) 101
AgsI TTSAA 2 cut(s) 187, 196
AluBI AGCT 3 cut(s) 52, 116, 214
AluI AGCT 3 cut(s) 52, 116, 214
AlwI GGATC 3 cut(s) 65, 78, 131
ApeKI GCWGC 2 cut(s) 82, 116
ApoI RAATTY 1 cut(s) 248
AsiGI ACCGGT 1 cut(s) 101
Asp700I GAANNNNTTC 1 cut(s) 191
BamHI GGATCC 1 cut(s) 70
BbvI GCAGC 2 cut(s) 94, 103
BcgI CGANNNNNNTGC 2 cut(s) 150, 184
BfaI CTAG 1 cut(s) 245
BfmI CTRYAG 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 119
BisI GCNGC 3 cut(s) 83, 117, 159
BlsI GCNGC 3 cut(s) 84, 118, 160
BmiI GGNNCC 1 cut(s) 72
BmsI GCATC 2 cut(s) 176, 246
BsaWI WCCGGW 1 cut(s) 101
Bse118I RCCGGY 3 cut(s) 58, 101, 119
BseRI GAGGAG 1 cut(s) 248
BseXI GCAGC 2 cut(s) 94, 103
BsgI GTGCAG 1 cut(s) 147
BshTI ACCGGT 1 cut(s) 101
BsiSI CCGG 3 cut(s) 59, 102, 120
BsmI GAATGC 1 cut(s) 35
Bsp1407I TGTACA 1 cut(s) 23
Bsp143I GATC 2 cut(s) 70, 136
BspACI CCGC 1 cut(s) 158
BspLI GGNNCC 1 cut(s) 72
BspMAI CTGCAG 1 cut(s) 84
BspMI ACCTGC 1 cut(s) 119
BspPI GGATC 3 cut(s) 65, 78, 131
BsrFI RCCGGY 3 cut(s) 58, 101, 119
BsrGI TGTACA 1 cut(s) 23
BssAI RCCGGY 3 cut(s) 58, 101, 119
BssMI GATC 2 cut(s) 70, 136
Bst4CI ACNGT 2 cut(s) 28, 192
BstAPI GCANNNNNTGC 1 cut(s) 155
BstAUI TGTACA 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 60
BstKTI GATC 2 cut(s) 73, 139
BstMBI GATC 2 cut(s) 70, 136
BstMWI GCNNNNNNNGC 3 cut(s) 125, 155, 164
BstSFI CTRYAG 1 cut(s) 80
BstV1I GCAGC 2 cut(s) 94, 103
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
BtgZI GCGATG 1 cut(s) 153
BtsIMutI CAGTG 1 cut(s) 33
BveI ACCTGC 1 cut(s) 119
Cac8I GCNNGC 1 cut(s) 60
Cfr10I RCCGGY 3 cut(s) 58, 101, 119
Csp6I GTAC 2 cut(s) 24, 202
CspAI ACCGGT 1 cut(s) 101
CviAII CATG 1 cut(s) 21
CviJI RGCY 4 cut(s) 52, 85, 116, 214
CviKI_1 RGCY 4 cut(s) 52, 85, 116, 214
CviQI GTAC 2 cut(s) 24, 202
DpnI GATC 2 cut(s) 72, 138
DpnII GATC 2 cut(s) 70, 136
FaeI CATG 1 cut(s) 24
FaiI YATR 5 cut(s) 22, 64, 113, 135, 206
FatI CATG 1 cut(s) 20
Fnu4HI GCNGC 3 cut(s) 83, 117, 159
Fsp4HI GCNGC 3 cut(s) 83, 117, 159
FspBI CTAG 1 cut(s) 245
GluI GCNGC 3 cut(s) 83, 117, 159
HapII CCGG 3 cut(s) 59, 102, 120
Hin1II CATG 1 cut(s) 24
HinfI GANTC 1 cut(s) 42
HpaII CCGG 3 cut(s) 59, 102, 120
Hpy188I TCNGA 3 cut(s) 41, 178, 255
HpyCH4III ACNGT 2 cut(s) 28, 192
HpyCH4V TGCA 3 cut(s) 82, 128, 167
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 155, 164
Hsp92II CATG 1 cut(s) 24
KroI GCCGGC 1 cut(s) 58
KroNI GCCGGC 1 cut(s) 60
Kzo9I GATC 2 cut(s) 70, 136
LmnI GCTCC 1 cut(s) 49
LpnPI CCDG 5 cut(s) 72, 92, 114, 115, 133
Lsp1109I GCAGC 2 cut(s) 94, 103
LweI GCATC 2 cut(s) 176, 246
MaeI CTAG 1 cut(s) 245
MaeIII GTNAC 1 cut(s) 3
MalI GATC 2 cut(s) 72, 138
MboI GATC 2 cut(s) 70, 136
MfeI CAATTG 1 cut(s) 182
MflI RGATCY 1 cut(s) 70
MluCI AATT 3 cut(s) 182, 222, 248
MmeI TCCRAC 2 cut(s) 98, 208
MnlI CCTC 2 cut(s) 104, 226
MroNI GCCGGC 1 cut(s) 58
MroXI GAANNNNTTC 1 cut(s) 191
MseI TTAA 1 cut(s) 262
MspI CCGG 3 cut(s) 59, 102, 120
MunI CAATTG 1 cut(s) 182
Mva1269I GAATGC 1 cut(s) 35
MwoI GCNNNNNNNGC 3 cut(s) 125, 155, 164
NaeI GCCGGC 1 cut(s) 60
NdeII GATC 2 cut(s) 70, 136
NgoMIV GCCGGC 1 cut(s) 58
NlaIII CATG 1 cut(s) 24
NlaIV GGNNCC 1 cut(s) 72
PctI GAATGC 1 cut(s) 35
PdiI GCCGGC 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 191
PfeI GAWTC 1 cut(s) 42
PinAI ACCGGT 1 cut(s) 101
PkrI GCNGC 3 cut(s) 84, 118, 160
PspN4I GGNNCC 1 cut(s) 72
PstI CTGCAG 1 cut(s) 84
PsuI RGATCY 1 cut(s) 70
RsaI GTAC 2 cut(s) 25, 203
RsaNI GTAC 2 cut(s) 24, 202
SaqAI TTAA 1 cut(s) 262
SatI GCNGC 3 cut(s) 83, 117, 159
Sau3AI GATC 2 cut(s) 70, 136
SetI ASST 5 cut(s) 54, 96, 118, 133, 216
SfaNI GCATC 2 cut(s) 176, 246
SfcI CTRYAG 1 cut(s) 80
Sse9I AATT 3 cut(s) 182, 222, 248
SsiI CCGC 1 cut(s) 158
SspMI CTAG 1 cut(s) 245
TaaI ACNGT 2 cut(s) 28, 192
TasI AATT 3 cut(s) 182, 222, 248
TatI WGTACW 2 cut(s) 23, 201
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 1 cut(s) 42
Tru1I TTAA 1 cut(s) 262
Tru9I TTAA 1 cut(s) 262
TscAI CASTG 1 cut(s) 33
TseI GCWGC 2 cut(s) 82, 116
TspDTI ATGAA 1 cut(s) 9
TspRI CASTG 1 cut(s) 33
XapI RAATTY 1 cut(s) 248
XmnI GAANNNNTTC 1 cut(s) 191
XspI CTAG 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.