Rh2BG127500

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0021153
Physical Location & Seq
Reverse (-)
10902511 .. 10903200
690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG127500.1

Sequence Viewer

Length: 417 bp
ATGTCAAGATCGACTATAGTCAAGTGCAATTTCCAACCTTATGGGCGGGACTACATAGGAAAAAAGCCTACCGGACGGTTTAGTAAAGGAAAAGTCCCCTCGGATTTTATAGCTGAAGAACTTGGAATCAAGAACACATTGCCAGCTTACCTGGACCCGAAGCTACAGATTCAAGATCTACTTACTGGTGTAAGTTTTGCCTCAGGTGGTTCAGGCTATGATCCACTGACACCCCAAATAGTGAATGTGTTATCATTATCTGATCAGTTAGACCTGTTCAAAGAGTACATAAGCAAGATAAATGCAGTCGTTGGGCCGGAAAGAACAGCTACCATAGTGTCGAAAAGCATATACATAATGTGCATAGGAAGCGATGACATTGCGAATACTTATTTCTCTACACCACTGACTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

138

Amino Acids

15.1

Weight (kDa)

7.71

Isoelectric Point (pI)

21.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 20 - 133 4e-08 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021153)

Species Orthologous Gene IDs
malus_domestica MD15G1009700.v1.1
pyrus_communis pycom01g13310
rosa_samantha Rh2AG124300 Rh2BG127500 Rh2CG128400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 46
AclWI GGATC 1 cut(s) 215
AcuI CTGAAG 1 cut(s) 135
AfaI GTAC 1 cut(s) 287
AgsI TTSAA 2 cut(s) 173, 280
AjnI CCWGG 1 cut(s) 150
AluBI AGCT 4 cut(s) 113, 146, 163, 329
AluI AGCT 4 cut(s) 113, 146, 163, 329
AlwI GGATC 1 cut(s) 215
AoxI GGCC 1 cut(s) 314
AspS9I GGNCC 2 cut(s) 154, 314
AvaII GGWCC 1 cut(s) 154
AxyI CCTNAGG 1 cut(s) 202
BcgI CGANNNNNNTGC 2 cut(s) 362, 396
BciT130I CCWGG 1 cut(s) 152
BclI TGATCA 1 cut(s) 262
BfmI CTRYAG 2 cut(s) 15, 164
BglII AGATCT 1 cut(s) 175
Bme1390I CCNGG 1 cut(s) 152
Bme18I GGWCC 1 cut(s) 154
BmgT120I GGNCC 2 cut(s) 154, 314
BmiI GGNNCC 1 cut(s) 156
BmrFI CCNGG 1 cut(s) 152
BoxI GACNNNNGTC 1 cut(s) 17
BsaJI CCNNGG 1 cut(s) 99
BsaWI WCCGGW 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 190
Bse21I CCTNAGG 1 cut(s) 202
Bse3DI GCAATG 2 cut(s) 137, 378
BseBI CCWGG 1 cut(s) 152
BseDI CCNNGG 1 cut(s) 99
BseMI GCAATG 2 cut(s) 137, 378
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 1 cut(s) 190
BshFI GGCC 1 cut(s) 316
BsiSI CCGG 2 cut(s) 72, 317
BslFI GGGAC 2 cut(s) 62, 80
BsmFI GGGAC 2 cut(s) 62, 80
BsnI GGCC 1 cut(s) 316
Bsp143I GATC 4 cut(s) 8, 175, 220, 262
BspACI CCGC 1 cut(s) 46
BspANI GGCC 1 cut(s) 316
BspCNI CTCAG 1 cut(s) 215
BspLI GGNNCC 1 cut(s) 156
BspPI GGATC 1 cut(s) 215
BsrDI GCAATG 2 cut(s) 137, 378
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 4 cut(s) 8, 175, 220, 262
Bst2UI CCWGG 1 cut(s) 152
Bst4CI ACNGT 1 cut(s) 78
BstC8I GCNNGC 1 cut(s) 144
BstDEI CTNAG 1 cut(s) 202
BstKTI GATC 4 cut(s) 11, 178, 223, 265
BstMBI GATC 4 cut(s) 8, 175, 220, 262
BstMWI GCNNNNNNNGC 1 cut(s) 369
BstNI CCWGG 1 cut(s) 152
BstPAI GACNNNNGTC 1 cut(s) 17
BstSCI CCNGG 1 cut(s) 150
BstSFI CTRYAG 2 cut(s) 15, 164
BstX2I RGATCY 1 cut(s) 175
BstXI CCANNNNNNTGG 1 cut(s) 41
BstYI RGATCY 1 cut(s) 175
Bsu36I CCTNAGG 1 cut(s) 202
BsuRI GGCC 1 cut(s) 316
BtgZI GCGATG 1 cut(s) 387
BtsIMutI CAGTG 3 cut(s) 224, 404, 412
Cac8I GCNNGC 1 cut(s) 144
Cfr13I GGNCC 2 cut(s) 154, 314
Csp6I GTAC 1 cut(s) 286
CviJI RGCY 7 cut(s) 67, 113, 146, 163, 216, 316, 329
CviKI_1 RGCY 7 cut(s) 67, 113, 146, 163, 216, 316, 329
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 1 cut(s) 202
DpnI GATC 4 cut(s) 10, 177, 222, 264
DpnII GATC 4 cut(s) 8, 175, 220, 262
Eco47I GGWCC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 135
Eco81I CCTNAGG 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 150
FalI AAGNNNNNCTT 2 cut(s) 165, 197
FaqI GGGAC 2 cut(s) 62, 80
FauI CCCGC 1 cut(s) 39
FbaI TGATCA 1 cut(s) 262
HaeIII GGCC 1 cut(s) 316
HapII CCGG 2 cut(s) 72, 317
HinfI GANTC 3 cut(s) 126, 169, 409
HpaII CCGG 2 cut(s) 72, 317
Hpy188I TCNGA 2 cut(s) 103, 262
Hpy188III TCNNGA 3 cut(s) 6, 130, 173
HpyCH4III ACNGT 1 cut(s) 78
HpyCH4V TGCA 3 cut(s) 27, 305, 363
HpyF10VI GCNNNNNNNGC 1 cut(s) 369
HpyF3I CTNAG 1 cut(s) 202
Ksp22I TGATCA 1 cut(s) 262
Kzo9I GATC 4 cut(s) 8, 175, 220, 262
LpnPI CCDG 9 cut(s) 85, 137, 156, 164, 171, 189, 198, 287, 330
MalI GATC 4 cut(s) 10, 177, 222, 264
MboI GATC 4 cut(s) 8, 175, 220, 262
MboII GAAGA 1 cut(s) 128
MflI RGATCY 1 cut(s) 175
MluCI AATT 1 cut(s) 28
MlyI GAGTC 1 cut(s) 403
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 2 cut(s) 109, 211
MspI CCGG 2 cut(s) 72, 317
MspR9I CCNGG 1 cut(s) 152
MvaI CCWGG 1 cut(s) 152
MwoI GCNNNNNNNGC 1 cut(s) 369
NdeII GATC 4 cut(s) 8, 175, 220, 262
NlaIV GGNNCC 1 cut(s) 156
PfeI GAWTC 2 cut(s) 126, 169
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
PshAI GACNNNNGTC 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 150
PspGI CCWGG 1 cut(s) 150
PspN4I GGNNCC 1 cut(s) 156
PspPI GGNCC 2 cut(s) 154, 314
PsuI RGATCY 1 cut(s) 175
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
Sau3AI GATC 4 cut(s) 8, 175, 220, 262
Sau96I GGNCC 2 cut(s) 154, 314
SchI GAGTC 1 cut(s) 403
ScrFI CCNGG 1 cut(s) 152
SetI ASST 8 cut(s) 40, 115, 148, 153, 165, 208, 276, 331
SfcI CTRYAG 2 cut(s) 15, 164
SinI GGWCC 1 cut(s) 154
Sse9I AATT 1 cut(s) 28
SsiI CCGC 1 cut(s) 46
StyD4I CCNGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 78
TaqI TCGA 2 cut(s) 11, 341
TasI AATT 1 cut(s) 28
TatI WGTACW 1 cut(s) 285
TfiI GAWTC 2 cut(s) 126, 169
TscAI CASTG 2 cut(s) 231, 411
TspRI CASTG 2 cut(s) 231, 411
VpaK11BI GGWCC 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.