Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0017255

Physical Location & Seq

Genomic Coordinates Reverse (-)

1517955 .. 1519895

Gene Structure 1941 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1026000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1941 bp

ATGAACTATGAGAATTATGACCCTTCCTATCCTGACCAGCCTGTGGTGGATCTCTACTTTCCGGTATGGGCTAGCCTCCCTGCCTTCCGGTCCAAGCCGGCCTTCGTTTGGGTTGAAGATGGCTCAGCCAATGAGGCCACTTCAACCCTAACATATGGCCAGATGAATGACTCAGTGCAATGCATTGCTTCTCAACTGCTCATCCCACTCCAAAGAGGTGACGCCGTTGTCATCCTATGCTCGCCAGGCCTCGAGCTCGTTGAGACCATTTTCGGGTGCCAACGAGCCGGCCTTTTGAGCGTGCCTGTTTCCCCGCCCGACCCTTCTTTCACTAACCAGAACTTCCACCACCTTATAAGAGCCCTCTCCCAAACAAAGCCTAAAGCTGCCCTAGCTCATTCTACTTACATCACAAGAATTCAGCATTACATATCTTTGCCATCCTCCGATAAAGATCTTGTCCAAAGATTGAAAAATCTTCGATGGATCGCCATAGACGACATCAAAACTAAAGATACAACGTTACAACATTCACCATACAATGGCTGCAGAGCAGAGGACTTGTATTTGGTTCAATACACTTCAGGTGCAACTGGGATCCCAAAGCCGGTGCTTGTCACGGCAGGATCAGCTGCACATAACGTTAGGACAGCGAGAAAGGCCTATGATCTTCACCCGAACAGCGTGATCGTGTCCTGGCTGCCGCAGTACCACGATTGCGGCCTAATGTTTCTGTTGCTAACAATTGTGTCCGGAGCCACATGTGTCTTGACATCGCCCGGCGCATTTGTTAACAGGCCACGGCTTTGGCTCGAGCTTATTTCGAACTTCAACGCCACTTGCACTCCAGTTCCGTCGTTCACATTGCCGCTCGTTGTCAAACGCGGAGGGGTTGACAAAGGAACTTCACCTATAAATCTATGGAGTATGAAAAATCTTATCATTATCAACGAGCCTATTTACCGCGACGCGGTCGAGCAATTTGTCGATGTGTTTAAGCCTTATGGATTAAACCCGTCATCAATTTCTCCATCATATGGCTTGGCAGAGAATTGCACGTTTGTTTCAACTGCGTGGCGATCAGACACGCCATTGCCATCCCACAACAAGCTCCTGCCGAGCGCAAGGCTCAACGAAAATGACGACCAGTTGGAGGACATGAACATTGTGGTAGTAAACGAAGAGACACACGAGGCAGTCGAGGATGGGATCGAAGGAGAGATTTGGGTTTCGTCTCCGAGCAATGCGTCTGGTTACTTAGCCCACCCAAATTTAACTCGTGAAGTTTATTACGGAAGACTTAAGAACAAGTTGAGTCGGCCATTTCTTCGAACTGGCGATAGGGGTGTCGTGAAAGGAGAAGATCGGTATCTTTTCGTGATGGGCCGATGCGTAGACGTGATCAGACTCCCAAACGACGACGTGGGGATGGAAATCCACCCTCATTACGTAGAGACGGTGGCGTATAACAGCAACCCGGGATTTCTGAGAGGTGGGTGTTTGGCGGCGTTTGAGGTTTCGAACACCGTTGTGGTGGTTGCGGAGACGCAGAGGATGGAGAAAAACATTGGAGTTTTGAGGAAAATATGTGACGGAGTGAGAAACGGTGTGGTTATGGAAGAGGGAGTGGAAGTTGGGACGGTGGTTCTGGTGAGAAGTGGGTGTGTGCCTAAAACGACGTCGGGTAAAATACAGAGATGGGCAGCTAAAGATAAGCTGGTTGGCGGGAAAATGGGAGTTTTGATGGAGGTGAGGTTTGGGGATTACGGTGGCTCTGTCTCAAGTTTGGGGGCTGAAGTTGGTGAGAGTGAGGGGAGAGGTGGCGAGAGAGGAGGAGGGGAAGGGTGGGGAAAAGTAGTGGTGGGTGAAGAGAGAGAAGAGATCTTTCTTTCGTTCTCATCAAATGCTTCAATTCGTCCCCCGTTGCAATCTTTTCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006629 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009273 GO:0009987 GO:0042546 GO:0044085 GO:0044238 GO:0071554 GO:0071704 GO:0071766 GO:0071840 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AMP-binding

Protein Analysis

647

Amino Acids

70.9

Weight (kDa)

5.88

Isoelectric Point (pI)

38.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	30 - 420	2.3e-54	AMP-binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017255)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g39700
malus_domestica	MD15G1026000.v1.1
prunus_persica	Prupe.1G378300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0301171
rosa_laevigata	RLG00000011271
rosa_multiflora	Rmu_sc0006701.1_g000005
rosa_roxburghii	Rroxscaffold_172G00445450 Rroxscaffold_7G00167090
rosa_rugosa	Rorug06G0302100
rosa_samantha	Rh6AG415400 Rh6BG422300 Rh6CG429900 Rh6DG415200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	356
AasI	GACNNNNNNGTC	1 cut(s)	227
AatII	GACGTC	1 cut(s)	1682
AccB1I	GGYRCC	1 cut(s)	276
AccB7I	CCANNNNNTGG	3 cut(s)	43, 542, 1037
AccBSI	CCGCTC	1 cut(s)	871
AccI	GTMKAC	1 cut(s)	1395
AccII	CGCG	3 cut(s)	885, 966, 971
AccIII	TCCGGA	1 cut(s)	752
AclI	AACGTT	2 cut(s)	521, 642
AclWI	GGATC	6 cut(s)	57, 494, 592, 605, 634, 1217
AcoI	YGGCCR	2 cut(s)	157, 1319
AcsI	RAATTY	2 cut(s)	417, 1270
AcuI	CTGAAG	2 cut(s)	567, 1815
AcyI	GRCGYC	2 cut(s)	222, 1679
AfaI	GTAC	1 cut(s)	710
AfiI	CCNNNNNNNGG	8 cut(s)	43, 108, 273, 542, 607, 970, 1037, 1153
AflII	CTTAAG	1 cut(s)	1301
AflIII	ACRYGT	1 cut(s)	761
AgsI	TTSAA	7 cut(s)	116, 144, 472, 575, 832, 1068, 1911
AjiI	CACGTC	2 cut(s)	1399, 1423
AjnI	CCWGG	2 cut(s)	244, 695
AjuI	GAANNNNNNNTTGG	2 cut(s)	86, 118
AleI	CACNNNNGTG	1 cut(s)	1529
AluBI	AGCT	8 cut(s)	256, 386, 395, 632, 817, 1111, 1706, 1717
AluI	AGCT	8 cut(s)	256, 386, 395, 632, 817, 1111, 1706, 1717
Alw21I	GWGCWC	1 cut(s)	258
Alw26I	GTCTC	6 cut(s)	257, 1178, 1239, 1448, 1538, 1783
AlwI	GGATC	6 cut(s)	57, 494, 592, 605, 634, 1217
Ama87I	CYCGRG	3 cut(s)	251, 812, 1477
Aor13HI	TCCGGA	1 cut(s)	752
ApeKI	GCWGC	5 cut(s)	386, 546, 632, 700, 1703
ApoI	RAATTY	2 cut(s)	417, 1270
AspLEI	GCGC	2 cut(s)	785, 1124
AspS9I	GGNCC	2 cut(s)	90, 1384
AsuC2I	CCSGG	3 cut(s)	780, 1478, 1479
AsuHPI	GGTGA	8 cut(s)	230, 525, 665, 900, 1663, 1762, 1814, 1877
AsuII	TTCGAA	3 cut(s)	824, 1330, 1520
AsuNHI	GCTAGC	1 cut(s)	71
AvaI	CYCGRG	3 cut(s)	251, 812, 1477
AvaII	GGWCC	1 cut(s)	90
BalI	TGGCCA	1 cut(s)	159
BamHI	GGATCC	1 cut(s)	597
BanI	GGYRCC	1 cut(s)	276
BanII	GRGCYC	2 cut(s)	258, 364
BauI	CACGAG	2 cut(s)	1190, 1278
BbsI	GAAGAC	1 cut(s)	1303
Bbv12I	GWGCWC	1 cut(s)	258
BbvI	GCAGC	5 cut(s)	373, 533, 619, 687, 1715
BceAI	ACGGC	3 cut(s)	209, 636, 818
BciT130I	CCWGG	2 cut(s)	246, 697
BclI	TGATCA	1 cut(s)	1401
BcnI	CCSGG	3 cut(s)	780, 1478, 1479
BcoDI	GTCTC	6 cut(s)	257, 1178, 1239, 1448, 1538, 1783
BfaI	CTAG	2 cut(s)	72, 392
BfmI	CTRYAG	1 cut(s)	547
BfrI	CTTAAG	1 cut(s)	1301
BglI	GCCNNNNNGGC	1 cut(s)	134
BglII	AGATCT	2 cut(s)	454, 1881
BisI	GCNGC	9 cut(s)	387, 547, 633, 701, 704, 721, 869, 1506, 1704
BlpI	GCTNAGC	1 cut(s)	124
BlsI	GCNGC	9 cut(s)	388, 548, 634, 702, 705, 722, 870, 1507, 1705
Bme1390I	CCNGG	5 cut(s)	246, 697, 780, 1478, 1479
Bme18I	GGWCC	1 cut(s)	90
BmeT110I	CYCGRG	3 cut(s)	251, 812, 1477
BmgBI	CACGTC	2 cut(s)	1399, 1423
BmgT120I	GGNCC	2 cut(s)	90, 1384
BmiI	GGNNCC	3 cut(s)	278, 599, 757
BmrFI	CCNGG	5 cut(s)	246, 697, 780, 1478, 1479
BmrI	ACTGGG	1 cut(s)	603
BmsI	GCATC	1 cut(s)	1379
BmtI	GCTAGC	1 cut(s)	75
BmuI	ACTGGG	1 cut(s)	603
BpiI	GAAGAC	1 cut(s)	1303
BpmI	CTGGAG	1 cut(s)	831
Bpu1102I	GCTNAGC	1 cut(s)	124
Bpu14I	TTCGAA	3 cut(s)	824, 1330, 1520
BpuEI	CTTGAG	1 cut(s)	1765
BpuMI	CCSGG	3 cut(s)	780, 1478, 1479
BsaAI	YACGTR	1 cut(s)	1450
BsaBI	GATNNNNATC	3 cut(s)	453, 1368, 1433
BsaHI	GRCGYC	2 cut(s)	222, 1679
BsaI	GGTCTC	1 cut(s)	257
BsaJI	CCNNGG	2 cut(s)	800, 1477
BsaWI	WCCGGW	3 cut(s)	61, 87, 752
BsaXI	ACNNNNNCTCC	6 cut(s)	829, 859, 1617, 1647, 1739, 1769
Bsc4I	CCNNNNNNNGG	8 cut(s)	43, 108, 273, 542, 607, 970, 1037, 1153
Bse118I	RCCGGY	3 cut(s)	97, 287, 607
Bse1I	ACTGG	4 cut(s)	598, 848, 1147, 1339
Bse3DI	GCAATG	5 cut(s)	183, 185, 863, 1091, 1249
Bse8I	GATNNNNATC	3 cut(s)	453, 1368, 1433
BseAI	TCCGGA	1 cut(s)	752
BseBI	CCWGG	2 cut(s)	246, 697
BseDI	CCNNGG	2 cut(s)	800, 1477
BseGI	GGATG	7 cut(s)	201, 231, 440, 1097, 1210, 1434, 1560
BseJI	GATNNNNATC	3 cut(s)	453, 1368, 1433
BseLI	CCNNNNNNNGG	8 cut(s)	43, 108, 273, 542, 607, 970, 1037, 1153
BseMI	GCAATG	5 cut(s)	183, 185, 863, 1091, 1249
BseMII	CTCAG	3 cut(s)	138, 186, 1478
BseNI	ACTGG	4 cut(s)	598, 848, 1147, 1339
BseRI	GAGGAG	2 cut(s)	1845, 1848
BseXI	GCAGC	5 cut(s)	373, 533, 619, 687, 1715
BsgI	GTGCAG	1 cut(s)	618
Bsh1236I	CGCG	3 cut(s)	885, 966, 971
Bsh1285I	CGRYCG	1 cut(s)	975
BshNI	GGYRCC	1 cut(s)	276
BsiEI	CGRYCG	1 cut(s)	975
BsiHKAI	GWGCWC	1 cut(s)	258
BsiHKCI	CYCGRG	3 cut(s)	251, 812, 1477
BsiSI	CCGG	8 cut(s)	62, 88, 98, 288, 608, 753, 780, 1478
BslFI	GGGAC	2 cut(s)	1651, 1902
BslI	CCNNNNNNNGG	8 cut(s)	43, 108, 273, 542, 607, 970, 1037, 1153
BsmAI	GTCTC	6 cut(s)	257, 1178, 1239, 1448, 1538, 1783
BsmBI	CGTCTC	3 cut(s)	1239, 1448, 1538
BsmFI	GGGAC	2 cut(s)	1651, 1902
Bso31I	GGTCTC	1 cut(s)	257
BsoBI	CYCGRG	3 cut(s)	251, 812, 1477
Bsp119I	TTCGAA	3 cut(s)	824, 1330, 1520
Bsp1286I	GDGCHC	2 cut(s)	258, 364
Bsp13I	TCCGGA	1 cut(s)	752
Bsp1720I	GCTNAGC	1 cut(s)	124
BspCNI	CTCAG	3 cut(s)	137, 185, 1479
BspEI	TCCGGA	1 cut(s)	752
BspFNI	CGCG	3 cut(s)	885, 966, 971
BspLI	GGNNCC	3 cut(s)	278, 599, 757
BspMAI	CTGCAG	1 cut(s)	551
BspOI	GCTAGC	1 cut(s)	75
BspPI	GGATC	6 cut(s)	57, 494, 592, 605, 634, 1217
BspT104I	TTCGAA	3 cut(s)	824, 1330, 1520
BspT107I	GGYRCC	1 cut(s)	276
BspTI	CTTAAG	1 cut(s)	1301
BspTNI	GGTCTC	1 cut(s)	257
BsrBI	CCGCTC	1 cut(s)	871
BsrDI	GCAATG	5 cut(s)	183, 185, 863, 1091, 1249
BsrFI	RCCGGY	3 cut(s)	97, 287, 607
BsrI	ACTGG	4 cut(s)	598, 848, 1147, 1339
BssAI	RCCGGY	3 cut(s)	97, 287, 607
BssECI	CCNNGG	2 cut(s)	800, 1477
BssNI	GRCGYC	2 cut(s)	222, 1679
BssSI	CACGAG	2 cut(s)	1190, 1278
Bst2BI	CACGAG	2 cut(s)	1190, 1278
Bst2UI	CCWGG	2 cut(s)	246, 697
Bst4CI	ACNGT	5 cut(s)	1459, 1528, 1607, 1642, 1769
Bst6I	CTCTTC	4 cut(s)	1176, 1614, 1863, 1872
BstACI	GRCGYC	2 cut(s)	222, 1679
BstAFI	CTTAAG	1 cut(s)	1301
BstBAI	YACGTR	1 cut(s)	1450
BstBI	TTCGAA	3 cut(s)	824, 1330, 1520
BstC8I	GCNNGC	5 cut(s)	73, 99, 242, 289, 302
BstDEI	CTNAG	4 cut(s)	124, 172, 1258, 1487
BstDSI	CCRYGG	1 cut(s)	800
BstF5I	GGATG	7 cut(s)	201, 231, 440, 1097, 1210, 1434, 1560
BstFNI	CGCG	3 cut(s)	885, 966, 971
BstHHI	GCGC	2 cut(s)	785, 1124
BstMAI	GTCTC	6 cut(s)	257, 1178, 1239, 1448, 1538, 1783
BstMCI	CGRYCG	1 cut(s)	975
BstMWI	GCNNNNNNNGC	6 cut(s)	134, 246, 297, 392, 629, 659
BstNI	CCWGG	2 cut(s)	246, 697
BstNSI	RCATGY	1 cut(s)	765
BstSCI	CCNGG	5 cut(s)	244, 695, 778, 1476, 1477
BstSFI	CTRYAG	1 cut(s)	547
BstSNI	TACGTA	1 cut(s)	1450
BstUI	CGCG	3 cut(s)	885, 966, 971
BstV1I	GCAGC	5 cut(s)	373, 533, 619, 687, 1715
BstV2I	GAAGAC	1 cut(s)	1303
BstX2I	RGATCY	4 cut(s)	49, 454, 597, 1881
BstXI	CCANNNNNNTGG	1 cut(s)	807
BstYI	RGATCY	4 cut(s)	49, 454, 597, 1881
BtgI	CCRYGG	1 cut(s)	800
BtgZI	GCGATG	1 cut(s)	759
BtrI	CACGTC	2 cut(s)	1399, 1423
BtsCI	GGATG	7 cut(s)	201, 231, 440, 1097, 1210, 1434, 1560
BtsIMutI	CAGTG	1 cut(s)	180
Cac8I	GCNNGC	5 cut(s)	73, 99, 242, 289, 302
CfoI	GCGC	2 cut(s)	785, 1124
Cfr10I	RCCGGY	3 cut(s)	97, 287, 607
Cfr13I	GGNCC	2 cut(s)	90, 1384
Cfr9I	CCCGGG	1 cut(s)	1477
CseI	GACGC	4 cut(s)	230, 977, 1236, 1555
Csp6I	GTAC	1 cut(s)	709
CviAII	CATG	2 cut(s)	762, 1159
CviQI	GTAC	1 cut(s)	709
DdeI	CTNAG	4 cut(s)	124, 172, 1258, 1487
DrdI	GACNNNNNNGTC	1 cut(s)	227
DseDI	GACNNNNNNGTC	1 cut(s)	227
EaeI	YGGCCR	2 cut(s)	157, 1319
Eam1104I	CTCTTC	4 cut(s)	1176, 1614, 1863, 1872
EarI	CTCTTC	4 cut(s)	1176, 1614, 1863, 1872
Ecl136II	GAGCTC	1 cut(s)	256
Eco105I	TACGTA	1 cut(s)	1450
Eco147I	AGGCCT	2 cut(s)	249, 662
Eco24I	GRGCYC	2 cut(s)	258, 364
Eco31I	GGTCTC	1 cut(s)	257
Eco47I	GGWCC	1 cut(s)	90
Eco53kI	GAGCTC	1 cut(s)	256
Eco57I	CTGAAG	2 cut(s)	567, 1815
Eco88I	CYCGRG	3 cut(s)	251, 812, 1477
EcoICRI	GAGCTC	1 cut(s)	256
EcoRI	GAATTC	1 cut(s)	417
EcoRII	CCWGG	2 cut(s)	244, 695
EcoT22I	ATGCAT	1 cut(s)	185
EcoT38I	GRGCYC	2 cut(s)	258, 364
Esp3I	CGTCTC	3 cut(s)	1239, 1448, 1538
FaeI	CATG	2 cut(s)	765, 1162
FalI	AAGNNNNNCTT	2 cut(s)	86, 118
FaqI	GGGAC	2 cut(s)	1651, 1902
FatI	CATG	2 cut(s)	761, 1158
FauI	CCCGC	2 cut(s)	321, 1718
FauNDI	CATATG	2 cut(s)	154, 1036
FbaI	TGATCA	1 cut(s)	1401
FblI	GTMKAC	1 cut(s)	1395
Fnu4HI	GCNGC	9 cut(s)	387, 547, 633, 701, 704, 721, 869, 1506, 1704
FokI	GGATG	7 cut(s)	188, 218, 427, 1084, 1217, 1441, 1567
FriOI	GRGCYC	2 cut(s)	258, 364
Fsp4HI	GCNGC	9 cut(s)	387, 547, 633, 701, 704, 721, 869, 1506, 1704
FspBI	CTAG	2 cut(s)	72, 392
GlaI	GCGC	2 cut(s)	784, 1123
GluI	GCNGC	9 cut(s)	387, 547, 633, 701, 704, 721, 869, 1506, 1704
GsuI	CTGGAG	1 cut(s)	831
HapII	CCGG	8 cut(s)	62, 88, 98, 288, 608, 753, 780, 1478
HgaI	GACGC	4 cut(s)	230, 977, 1236, 1555
HhaI	GCGC	2 cut(s)	785, 1124
Hin1I	GRCGYC	2 cut(s)	222, 1679
Hin1II	CATG	2 cut(s)	765, 1162
Hin6I	GCGC	2 cut(s)	783, 1122
HinP1I	GCGC	2 cut(s)	783, 1122
HincII	GTYRAC	2 cut(s)	793, 895
HindII	GTYRAC	2 cut(s)	793, 895
HinfI	GANTC	3 cut(s)	170, 1315, 1407
HpaI	GTTAAC	1 cut(s)	793
HpaII	CCGG	8 cut(s)	62, 88, 98, 288, 608, 753, 780, 1478
HphI	GGTGA	8 cut(s)	230, 525, 665, 900, 1663, 1762, 1814, 1877
Hpy166II	GTNNAC	5 cut(s)	793, 861, 895, 1177, 1396
Hpy188I	TCNGA	5 cut(s)	448, 1084, 1239, 1406, 1488
Hpy188III	TCNNGA	6 cut(s)	32, 753, 769, 1280, 1351, 1378
Hpy8I	GTNNAC	5 cut(s)	793, 861, 895, 1177, 1396
Hpy99I	CGWCG	6 cut(s)	859, 971, 1421, 1424, 1681, 1684
HpyAV	CCTTC	6 cut(s)	33, 94, 112, 333, 1208, 1835
HpyCH4III	ACNGT	5 cut(s)	1459, 1528, 1607, 1642, 1769
HpyCH4IV	ACGT	7 cut(s)	521, 642, 1058, 1398, 1422, 1449, 1679
HpyCH4V	TGCA	8 cut(s)	178, 183, 549, 590, 635, 843, 1056, 1927
HpyF10VI	GCNNNNNNNGC	6 cut(s)	134, 246, 297, 392, 629, 659
HpyF3I	CTNAG	4 cut(s)	124, 172, 1258, 1487
HpySE526I	ACGT	7 cut(s)	521, 642, 1058, 1398, 1422, 1449, 1679
Hsp92I	GRCGYC	2 cut(s)	222, 1679
Hsp92II	CATG	2 cut(s)	765, 1162
HspAI	GCGC	2 cut(s)	783, 1122
Kpn2I	TCCGGA	1 cut(s)	752
KroI	GCCGGC	2 cut(s)	97, 287
KroNI	GCCGGC	2 cut(s)	99, 289
Ksp22I	TGATCA	1 cut(s)	1401
KspAI	GTTAAC	1 cut(s)	793
LmnI	GCTCC	2 cut(s)	755, 1116
Lsp1109I	GCAGC	5 cut(s)	373, 533, 619, 687, 1715
LweI	GCATC	1 cut(s)	1379
MaeI	CTAG	2 cut(s)	72, 392
MaeII	ACGT	7 cut(s)	521, 642, 1058, 1398, 1422, 1449, 1679
MaeIII	GTNAC	5 cut(s)	218, 522, 616, 1253, 1589
MbiI	CCGCTC	1 cut(s)	871
MfeI	CAATTG	1 cut(s)	744
MflI	RGATCY	4 cut(s)	49, 454, 597, 1881
MhlI	GDGCHC	2 cut(s)	258, 364
MlsI	TGGCCA	1 cut(s)	159
MluCI	AATT	8 cut(s)	13, 417, 744, 980, 1023, 1051, 1270, 1911
MluNI	TGGCCA	1 cut(s)	159
MlyI	GAGTC	3 cut(s)	164, 1324, 1401
MmeI	TCCRAC	1 cut(s)	1131
Mox20I	TGGCCA	1 cut(s)	159
Mph1103I	ATGCAT	1 cut(s)	185
MroI	TCCGGA	1 cut(s)	752
MroNI	GCCGGC	2 cut(s)	97, 287
MscI	TGGCCA	1 cut(s)	159
MseI	TTAA	5 cut(s)	792, 996, 1010, 1274, 1302
MslI	CAYNNNNRTG	1 cut(s)	1529
Msp20I	TGGCCA	1 cut(s)	159
MspA1I	CMGCKG	1 cut(s)	632
MspCI	CTTAAG	1 cut(s)	1301
MspI	CCGG	8 cut(s)	62, 88, 98, 288, 608, 753, 780, 1478
MspR9I	CCNGG	5 cut(s)	246, 697, 780, 1478, 1479
MunI	CAATTG	1 cut(s)	744
MvaI	CCWGG	2 cut(s)	246, 697
MvnI	CGCG	3 cut(s)	885, 966, 971
MwoI	GCNNNNNNNGC	6 cut(s)	134, 246, 297, 392, 629, 659
NaeI	GCCGGC	2 cut(s)	99, 289
NciI	CCSGG	3 cut(s)	780, 1478, 1479
NdeI	CATATG	2 cut(s)	154, 1036
NgoMIV	GCCGGC	2 cut(s)	97, 287
NheI	GCTAGC	1 cut(s)	71
NlaIII	CATG	2 cut(s)	765, 1162
NlaIV	GGNNCC	3 cut(s)	278, 599, 757
NmeAIII	GCCGAG	1 cut(s)	1143
NmuCI	GTSAC	3 cut(s)	218, 616, 1589
NsiI	ATGCAT	1 cut(s)	185
NspI	RCATGY	1 cut(s)	765
NspV	TTCGAA	3 cut(s)	824, 1330, 1520
OliI	CACNNNNGTG	1 cut(s)	1529
PaeR7I	CTCGAG	2 cut(s)	251, 812
PceI	AGGCCT	2 cut(s)	249, 662
PciI	ACATGT	1 cut(s)	761
PcsI	WCGNNNNNNNCGW	3 cut(s)	495, 1140, 1197
PdiI	GCCGGC	2 cut(s)	99, 289
PflFI	GACNNNGTC	1 cut(s)	971
PflMI	CCANNNNNTGG	3 cut(s)	43, 542, 1037
PkrI	GCNGC	9 cut(s)	388, 548, 634, 702, 705, 722, 870, 1507, 1705
PleI	GAGTC	3 cut(s)	164, 1323, 1401
PpsI	GAGTC	3 cut(s)	164, 1323, 1401
Ppu21I	YACGTR	1 cut(s)	1450
PscI	ACATGT	1 cut(s)	761
PsiI	TTATAA	1 cut(s)	356
Psp124BI	GAGCTC	1 cut(s)	258
Psp1406I	AACGTT	2 cut(s)	521, 642
Psp6I	CCWGG	2 cut(s)	244, 695
PspGI	CCWGG	2 cut(s)	244, 695
PspN4I	GGNNCC	3 cut(s)	278, 599, 757
PspPI	GGNCC	2 cut(s)	90, 1384
PspXI	VCTCGAGB	2 cut(s)	251, 812
PstI	CTGCAG	1 cut(s)	551
PsuI	RGATCY	4 cut(s)	49, 454, 597, 1881
PsyI	GACNNNGTC	1 cut(s)	971
PvuII	CAGCTG	1 cut(s)	632
RsaI	GTAC	1 cut(s)	710
RsaNI	GTAC	1 cut(s)	709
RseI	CAYNNNNRTG	1 cut(s)	1529
SacI	GAGCTC	1 cut(s)	258
SaqAI	TTAA	5 cut(s)	792, 996, 1010, 1274, 1302
SatI	GCNGC	9 cut(s)	387, 547, 633, 701, 704, 721, 869, 1506, 1704
Sau96I	GGNCC	2 cut(s)	90, 1384
SchI	GAGTC	3 cut(s)	164, 1324, 1401
ScrFI	CCNGG	5 cut(s)	246, 697, 780, 1478, 1479
SduI	GDGCHC	2 cut(s)	258, 364
SfaNI	GCATC	1 cut(s)	1379
SfcI	CTRYAG	1 cut(s)	547
Sfr274I	CTCGAG	2 cut(s)	251, 812
SfuI	TTCGAA	3 cut(s)	824, 1330, 1520
SinI	GGWCC	1 cut(s)	90
SlaI	CTCGAG	2 cut(s)	251, 812
SmaI	CCCGGG	1 cut(s)	1479
SmiMI	CAYNNNNRTG	1 cut(s)	1529
SmlI	CTYRAG	4 cut(s)	251, 812, 1301, 1780
SmoI	CTYRAG	4 cut(s)	251, 812, 1301, 1780
SnaBI	TACGTA	1 cut(s)	1450
Sse9I	AATT	8 cut(s)	13, 417, 744, 980, 1023, 1051, 1270, 1911
SseBI	AGGCCT	2 cut(s)	249, 662
SspMI	CTAG	2 cut(s)	72, 392
SstI	GAGCTC	1 cut(s)	258
StuI	AGGCCT	2 cut(s)	249, 662
StyD4I	CCNGG	5 cut(s)	244, 695, 778, 1476, 1477
TaaI	ACNGT	5 cut(s)	1459, 1528, 1607, 1642, 1769
TaiI	ACGT	7 cut(s)	524, 645, 1061, 1401, 1425, 1452, 1682
TasI	AATT	8 cut(s)	13, 417, 744, 980, 1023, 1051, 1270, 1911
TauI	GCSGC	4 cut(s)	706, 723, 871, 1508
Tru1I	TTAA	5 cut(s)	792, 996, 1010, 1274, 1302
Tru9I	TTAA	5 cut(s)	792, 996, 1010, 1274, 1302
TscAI	CASTG	1 cut(s)	180
TseFI	GTSAC	3 cut(s)	218, 616, 1589
TseI	GCWGC	5 cut(s)	386, 546, 632, 700, 1703
Tsp45I	GTSAC	3 cut(s)	218, 616, 1589
TspDTI	ATGAA	4 cut(s)	17, 179, 944, 1175
TspGWI	ACGGA	3 cut(s)	843, 1308, 1608
TspMI	CCCGGG	1 cut(s)	1477
TspRI	CASTG	1 cut(s)	180
Tth111I	GACNNNGTC	1 cut(s)	971
Van91I	CCANNNNNTGG	3 cut(s)	43, 542, 1037
Vha464I	CTTAAG	1 cut(s)	1301
VpaK11BI	GGWCC	1 cut(s)	90
XapI	RAATTY	2 cut(s)	417, 1270
XceI	RCATGY	1 cut(s)	765
XhoI	CTCGAG	2 cut(s)	251, 812
XmaI	CCCGGG	1 cut(s)	1477
XmiI	GTMKAC	1 cut(s)	1395
XspI	CTAG	2 cut(s)	72, 392
ZraI	GACGTC	1 cut(s)	1680
Zsp2I	ATGCAT	1 cut(s)	185

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.