Rorug06G0302100

Disco-interacting protein 2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007076
Physical Location & Seq
Forward (+)
45339733 .. 45343281
3549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0302100.1

Sequence Viewer

Length: 615 bp
ATGGCCGGAAATATTTCAGAATCTTTGAAAGGTAAAAAGCGCGCGCGAGAATCCGACGCCGAACCCGAAACCGCCGATATCGATCATATTCTATCGTTAGAGGAAAATCTTACGTTTAGTGATACATTGGTGGCTCTTCGCATAATGCGAGAACAGTTTCCGCAAATTGACAAGCCAATTCAGCCTTTCATTTTGCGGTCACAGTTGTATAGCAGTGTAGAGGACAGGACACAAGTGGACCGGGACCTGGAGACTCTAAGAAGGGAGAAAGTGTTGCGCATTTTTAAATTAAATACGGGGCAAGATGATCATGCCATAATGTTTTTGGATGACTACCTATGCCAGGTAGACCGAGTTGTGAAAAGATTGGAAGGAGAAAAGGGAAGAGATGTTCTTGAAGTTTTTAACTGGTTCAGATCACACGTTATTGATTTCAAGCTTGAACCTAGTATTGAACACCAAGAACTTTGTACATTATTATCACTTGGAGGAAAGGTTAAAGATGAGCACATCTCACTTTTAATGAATTCTGGCCTTCTTGGATTGCTTAAGTGTTGTTTGTTGGATTTGTTTCAAACAATTTTGATGGCCTTCAGCTTTCTAACTGTCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006629 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009273 GO:0009987 GO:0042546 GO:0044085 GO:0044238 GO:0071554 GO:0071704 GO:0071766 GO:0071840 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding

Protein Analysis

204

Amino Acids

23.51

Weight (kDa)

5.2

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stk19 PF10494 36 - 180 1.1e-12 Inactive serine-threonine protein kinase 19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 278
AccI GTMKAC 1 cut(s) 348
AccII CGCG 3 cut(s) 42, 44, 46
AciI CCGC 3 cut(s) 72, 161, 196
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 526
AcuI CTGAAG 1 cut(s) 577
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 1 cut(s) 472
AfiI CCNNNNNNNGG 2 cut(s) 247, 343
AflII CTTAAG 1 cut(s) 548
AflIII ACRYGT 1 cut(s) 421
AgsI TTSAA 6 cut(s) 28, 398, 436, 443, 455, 575
AjnI CCWGG 2 cut(s) 246, 342
AloI GAACNNNNNNTCC 2 cut(s) 375, 407
AluBI AGCT 2 cut(s) 439, 597
AluI AGCT 2 cut(s) 439, 597
Alw21I GWGCWC 1 cut(s) 510
Alw26I GTCTC 1 cut(s) 245
AoxI GGCC 3 cut(s) 3, 532, 588
ApoI RAATTY 1 cut(s) 526
Asp700I GAANNNNTTC 2 cut(s) 13, 156
AspLEI GCGC 4 cut(s) 42, 44, 46, 279
AspS9I GGNCC 2 cut(s) 238, 244
AsuC2I CCSGG 1 cut(s) 242
AvaII GGWCC 2 cut(s) 238, 244
BaeI ACNNNNGTAYC 2 cut(s) 114, 147
Bbv12I GWGCWC 1 cut(s) 510
BccI CCATC 1 cut(s) 580
BciT130I CCWGG 2 cut(s) 248, 344
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 1 cut(s) 242
BcoDI GTCTC 1 cut(s) 245
BfaI CTAG 1 cut(s) 447
BfrI CTTAAG 1 cut(s) 548
Bme1390I CCNGG 3 cut(s) 242, 248, 344
Bme18I GGWCC 2 cut(s) 238, 244
BmgT120I GGNCC 2 cut(s) 238, 244
BmiI GGNNCC 1 cut(s) 245
BmrFI CCNGG 3 cut(s) 242, 248, 344
BplI GAGNNNNNCTC 2 cut(s) 497, 529
BpmI CTGGAG 1 cut(s) 269
BpuMI CCSGG 1 cut(s) 242
Bsa29I ATCGAT 1 cut(s) 81
BsaBI GATNNNNATC 1 cut(s) 81
BsaHI GRCGYC 1 cut(s) 57
BsaXI ACNNNNNCTCC 4 cut(s) 257, 287, 480, 510
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 343
Bse1I ACTGG 1 cut(s) 413
Bse8I GATNNNNATC 1 cut(s) 81
BseBI CCWGG 2 cut(s) 248, 344
BseCI ATCGAT 1 cut(s) 81
BseGI GGATG 1 cut(s) 334
BseJI GATNNNNATC 1 cut(s) 81
BseLI CCNNNNNNNGG 2 cut(s) 247, 343
BseNI ACTGG 1 cut(s) 413
BsePI GCGCGC 2 cut(s) 40, 42
Bsh1236I CGCG 3 cut(s) 42, 44, 46
BshFI GGCC 3 cut(s) 5, 534, 590
BshVI ATCGAT 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 510
BsiSI CCGG 2 cut(s) 6, 241
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 2 cut(s) 247, 343
BsmAI GTCTC 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 257
BsnI GGCC 3 cut(s) 5, 534, 590
Bsp1286I GDGCHC 1 cut(s) 510
Bsp1407I TGTACA 1 cut(s) 470
Bsp143I GATC 3 cut(s) 82, 307, 416
BspACI CCGC 3 cut(s) 72, 161, 196
BspANI GGCC 3 cut(s) 5, 534, 590
BspDI ATCGAT 1 cut(s) 81
BspFNI CGCG 3 cut(s) 42, 44, 46
BspLI GGNNCC 1 cut(s) 245
BspQI GCTCTTC 1 cut(s) 141
BspTI CTTAAG 1 cut(s) 548
BsrGI TGTACA 1 cut(s) 470
BsrI ACTGG 1 cut(s) 413
BssHII GCGCGC 2 cut(s) 40, 42
BssMI GATC 3 cut(s) 82, 307, 416
BssNI GRCGYC 1 cut(s) 57
Bst2UI CCWGG 2 cut(s) 248, 344
Bst4CI ACNGT 3 cut(s) 156, 204, 607
Bst6I CTCTTC 2 cut(s) 141, 379
BstACI GRCGYC 1 cut(s) 57
BstAFI CTTAAG 1 cut(s) 548
BstAUI TGTACA 1 cut(s) 470
BstC8I GCNNGC 2 cut(s) 42, 44
BstDEI CTNAG 1 cut(s) 257
BstENI CCTNNNNNAGG 1 cut(s) 341
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 3 cut(s) 42, 44, 46
BstHHI GCGC 4 cut(s) 42, 44, 46, 279
BstKTI GATC 3 cut(s) 85, 310, 419
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 3 cut(s) 82, 307, 416
BstMWI GCNNNNNNNGC 1 cut(s) 181
BstNI CCWGG 2 cut(s) 248, 344
BstSCI CCNGG 3 cut(s) 240, 246, 342
BstUI CGCG 3 cut(s) 42, 44, 46
Bsu15I ATCGAT 1 cut(s) 81
BsuRI GGCC 3 cut(s) 5, 534, 590
BsuTUI ATCGAT 1 cut(s) 81
BtsCI GGATG 1 cut(s) 334
BtsI GCAGTG 1 cut(s) 220
BtsIMutI CAGTG 1 cut(s) 220
Cac8I GCNNGC 2 cut(s) 42, 44
CfoI GCGC 4 cut(s) 42, 44, 46, 279
Cfr13I GGNCC 2 cut(s) 238, 244
ClaI ATCGAT 1 cut(s) 81
CseI GACGC 1 cut(s) 65
Csp6I GTAC 1 cut(s) 471
CviAII CATG 2 cut(s) 311, 612
CviJI RGCY 8 cut(s) 5, 134, 175, 184, 439, 534, 590, 597
CviKI_1 RGCY 8 cut(s) 5, 134, 175, 184, 439, 534, 590, 597
CviQI GTAC 1 cut(s) 471
DdeI CTNAG 1 cut(s) 257
DpnI GATC 3 cut(s) 84, 309, 418
DpnII GATC 3 cut(s) 82, 307, 416
DraI TTTAAA 1 cut(s) 286
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 141, 379
EarI CTCTTC 2 cut(s) 141, 379
Eco32I GATATC 1 cut(s) 79
Eco47I GGWCC 2 cut(s) 238, 244
Eco57I CTGAAG 1 cut(s) 577
EcoNI CCTNNNNNAGG 1 cut(s) 341
EcoO109I RGGNCCY 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 526
EcoRII CCWGG 2 cut(s) 246, 342
EcoRV GATATC 1 cut(s) 79
FaeI CATG 2 cut(s) 314, 615
FaiI YATR 7 cut(s) 87, 143, 210, 312, 317, 340, 613
FaqI GGGAC 1 cut(s) 257
FatI CATG 2 cut(s) 310, 611
FbaI TGATCA 1 cut(s) 307
FblI GTMKAC 1 cut(s) 348
FokI GGATG 1 cut(s) 341
FspBI CTAG 1 cut(s) 447
FspI TGCGCA 1 cut(s) 278
GlaI GCGC 4 cut(s) 41, 43, 45, 278
GsuI CTGGAG 1 cut(s) 269
HaeIII GGCC 3 cut(s) 5, 534, 590
HapII CCGG 2 cut(s) 6, 241
HgaI GACGC 1 cut(s) 65
HhaI GCGC 4 cut(s) 42, 44, 46, 279
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 2 cut(s) 314, 615
Hin6I GCGC 4 cut(s) 40, 42, 44, 277
HinP1I GCGC 4 cut(s) 40, 42, 44, 277
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 3 cut(s) 20, 50, 253
HpaII CCGG 2 cut(s) 6, 241
Hpy166II GTNNAC 2 cut(s) 238, 349
Hpy188I TCNGA 3 cut(s) 19, 55, 416
Hpy188III TCNNGA 1 cut(s) 395
Hpy8I GTNNAC 2 cut(s) 238, 349
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 4 cut(s) 255, 365, 545, 601
HpyCH4III ACNGT 3 cut(s) 156, 204, 607
HpyCH4IV ACGT 2 cut(s) 113, 423
HpyF10VI GCNNNNNNNGC 1 cut(s) 181
HpyF3I CTNAG 1 cut(s) 257
HpySE526I ACGT 2 cut(s) 113, 423
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 2 cut(s) 314, 615
HspAI GCGC 4 cut(s) 40, 42, 44, 277
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 3 cut(s) 82, 307, 416
LguI GCTCTTC 1 cut(s) 141
LpnPI CCDG 9 cut(s) 19, 211, 233, 254, 260, 329, 356, 394, 516
MaeI CTAG 1 cut(s) 447
MaeII ACGT 2 cut(s) 113, 423
MaeIII GTNAC 2 cut(s) 198, 607
MalI GATC 3 cut(s) 84, 309, 418
MauBI CGCGCGCG 1 cut(s) 42
MboI GATC 3 cut(s) 82, 307, 416
MboII GAAGA 2 cut(s) 128, 396
MhlI GDGCHC 1 cut(s) 510
MluCI AATT 5 cut(s) 165, 177, 287, 526, 579
MlyI GAGTC 1 cut(s) 247
MmeI TCCRAC 2 cut(s) 78, 543
MnlI CCTC 3 cut(s) 94, 214, 482
MroXI GAANNNNTTC 2 cut(s) 13, 156
MseI TTAA 6 cut(s) 285, 290, 405, 498, 521, 549
MspCI CTTAAG 1 cut(s) 548
MspI CCGG 2 cut(s) 6, 241
MspR9I CCNGG 3 cut(s) 242, 248, 344
MvaI CCWGG 2 cut(s) 248, 344
MvnI CGCG 3 cut(s) 42, 44, 46
MwoI GCNNNNNNNGC 1 cut(s) 181
NciI CCSGG 1 cut(s) 242
NdeII GATC 3 cut(s) 82, 307, 416
NlaIII CATG 2 cut(s) 314, 615
NlaIV GGNNCC 1 cut(s) 245
NmuCI GTSAC 2 cut(s) 198, 607
NsbI TGCGCA 1 cut(s) 278
PauI GCGCGC 2 cut(s) 40, 42
PciSI GCTCTTC 1 cut(s) 141
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 145
PdmI GAANNNNTTC 2 cut(s) 13, 156
PfeI GAWTC 2 cut(s) 20, 50
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
PpuMI RGGWCCY 1 cut(s) 244
Psp5II RGGWCCY 1 cut(s) 244
Psp6I CCWGG 2 cut(s) 246, 342
PspGI CCWGG 2 cut(s) 246, 342
PspN4I GGNNCC 1 cut(s) 245
PspPI GGNCC 2 cut(s) 238, 244
PspPPI RGGWCCY 1 cut(s) 244
PteI GCGCGC 2 cut(s) 40, 42
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
SapI GCTCTTC 1 cut(s) 141
SaqAI TTAA 6 cut(s) 285, 290, 405, 498, 521, 549
Sau3AI GATC 3 cut(s) 82, 307, 416
Sau96I GGNCC 2 cut(s) 238, 244
SchI GAGTC 1 cut(s) 247
ScrFI CCNGG 3 cut(s) 242, 248, 344
SduI GDGCHC 1 cut(s) 510
SinI GGWCC 2 cut(s) 238, 244
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
Sse9I AATT 5 cut(s) 165, 177, 287, 526, 579
SsiI CCGC 3 cut(s) 72, 161, 196
SspI AATATT 1 cut(s) 13
SspMI CTAG 1 cut(s) 447
StyD4I CCNGG 3 cut(s) 240, 246, 342
TaaI ACNGT 3 cut(s) 156, 204, 607
TaiI ACGT 2 cut(s) 116, 426
TaqI TCGA 1 cut(s) 81
TaqII GACCGA 1 cut(s) 366
TasI AATT 5 cut(s) 165, 177, 287, 526, 579
TatI WGTACW 1 cut(s) 470
TfiI GAWTC 2 cut(s) 20, 50
Tru1I TTAA 6 cut(s) 285, 290, 405, 498, 521, 549
Tru9I TTAA 6 cut(s) 285, 290, 405, 498, 521, 549
TscAI CASTG 1 cut(s) 220
TseFI GTSAC 2 cut(s) 198, 607
Tsp45I GTSAC 2 cut(s) 198, 607
TspDTI ATGAA 2 cut(s) 178, 539
TspRI CASTG 1 cut(s) 220
Vha464I CTTAAG 1 cut(s) 548
VpaK11BI GGWCC 2 cut(s) 238, 244
XagI CCTNNNNNAGG 1 cut(s) 341
XapI RAATTY 1 cut(s) 526
XcmI CCANNNNNNNNNTGG 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 348
XmnI GAANNNNTTC 2 cut(s) 13, 156
XspI CTAG 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.